Browsing by Author "Gumustepe E."

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    Biocompatibility of vertically aligned multi-walled carbon nanotube scaffolds for human breast cancer cell line MDA-MB-231
    (SpringerOpen, 2017) Akinoglu E.M.; Ozbilgin K.; Sonmez P.K.; Ozkut M.M.; Giersig M.; Inan S.; Gumustepe E.; Kurtman C.
    The aim of the current study was to determine whether the MWCNT-based scaffold has a suitable structure for cell growth and provides a biocompatible environment for human MDA-MB-231 cell lines. MWCNT-based nanostructured scaffolds were produced by plasma-enhanced chemical vapor deposition (PECVD) technique. MDA-MB-231 cells were seeded on MWCNTs-textured silicon scaffolds and on pristine silicon surfaces. After 1 week of culturing, the scaffolds were prepared for SEM analysis and immunocytochemical staining was performed for the two groups (MWCNT scaffold and pristine silicon surface), using MMP-2, MMP-9, PI3K, AKT and NF-κB primary antibodies. SEM analyses showed that the MDA-MB-231 cells better adhered to the MWCNT-based nanostructured scaffold than the pristine silicon surface. Immunohistochemical activity of the MDA-MB-231 cells on both materials has similar staining with anti-AKT MMP-2, MMP-9 and NF-κB primary antibodies. Therefore, the results of the present study suggest that the MWCNT-based scaffolds enhanced cell adhesion to the scaffold and exhibited more biomimetic properties and physiological adaptation with the potential to be used for in vitro metastasis studies of BrCa cell lines. © 2017, The Author(s).
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    PTX3 levels in murine pulmonary parenchymal tissues are correlated with radiation-induced injurie
    (Novin Medical Radiation Institute, 2020) Sarper B.; Ozbilgin M.K.; Gumustepe E.; Gencur S.; Karaman G.Z.; Kilicaslan P.; Kurtman C.
    Background: Pentraxins (PTX) play key roles in innate immunity and inflammatory responses. An increase in PTX3 levels may be a marker of early radiation injury in the lung. Thus, we aimed to determine the effect of radiation on PTX3 expression in a lung injury mouse model. Materials and Methods: Twenty-four 6–8-week-old mice were divided into 4 groups, one control (group 1) and three experimental groups (groups 2–4) irradiated with 6 MV photons and 5 Gy in a single fraction. Groups 2, 3, and 4 were sedated and euthanized 24, 72, and 168 h after radiation, respectively. The right lung middle lobe was then removed for histochemical examination and immunostaining for PTX3 expression, which was evaluated semi-quantitatively using H-SCORE analysis. Kolmogorov-Smirnov and Kruskal Wallis one-way analysis of variance were used for statistical analysis. Results: Immunohistochemistry of lung tissue samples showed different PTX3 expression levels across the four groups. Group 1 showed weak staining (232.50 ± 9.501), while group 2 (301.50 ± 7.472) and group 3 (283.50 ± 7.090) showed strong immunoreactivity. Group 4 showed moderate PTX3 immunoreactivity (271.50 ± 10.013). Moreover, H-score values between control and early radiation groups were statistically significant (group 1 vs. group 2, p < 0.001; group 1 vs. group 3, p = 0.002). Conclusion: PTX3 levels may be an early marker for long-term radiation effects. Our study provides insights into the pathological processes of pulmonary inflammation and acute radiation injury, and may provide novel therapeutic strategies for controlling pulmonary inflammation without eliciting radiation injury. © 2020 Novin Medical Radiation Institute. All rights reserved.
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    Effects of adrenomeduline and ramp2 on the lung of mice exposed to total body radiation
    (Novin Medical Radiation Institute, 2020) Ozbilgin M.K.; Karaman G.Z.; Gencur S.; Gumustepe E.; Kurtman C.
    Background: Adrenomedullin (AM) and its receptor, receptor actvity-modifying protein (RAMP) 2 have pleiotropic regulatory functons in normal tssue and cancer tssue. AM is produced and secreted both numerous stromal cells and tumor cells. This study aims to investgate a possible role of AM and RAMP2 in the radiaton exposure in the normal lung tssue. M aterial s and Methods: Four groups with 6 male adult Swiss Albino mice per group were investgated. The mice were subjected to a 500 cGy single-dose radiaton exposure in the total body radiaton device and lung tssues were collected. 1, 2, and 7 days after radiaton exposure, with 1 reference group which was not exposed to radiaton. Results: The general histology and the immunohistochemistry of the tssue samples prepared with ant-AM, ant-RAMP2, and monoclonal antbodies were investgated, yielding a statstcally significant increase for AM on day 3 and for RAMP2 on day 1 after radiaton exposure. Conclusion: The observed increase of AM and RAMP2 concentratons in the normal tssue matrix after radiaton exposure may play a role in the side effects of radiotherapy. © 2020 Novin Medical Radiation Institute. All rights reserved.