Browsing by Author "Gurcu B."

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    Histopathological and ultrastructural effects of Losartan on embryonic rat kidney
    (Elsevier GmbH, 2005) Akil I.; Inan S.; Gurcu B.; Nazikoglu A.; Ozbilgin K.; Muftuoglu S.
    The aim of our study was to investigate the histopathological, immunohistochemical and ultrastructural effects of Losartan (a selective angiotensin II type-1 receptor blocker) on renal development in rats. Twelve pregnant rats were divided into control and experimental groups. In the experimental group, Losartan (10 mg/kg/day) was given via nasogastric tube, between the sixth day of implantation and time of sacrifice on embryonic days 18 and 20. All formalin-fixed, paraffin wax-embedded renal tissue sections were stained with hematoxylin and eosin or labelled for binding of primary antibodies against transforming growth factor-β (TGF-β 1,-2,-3) using an avidin-biotin-peroxidase method. For electron microscopic examination, samples were fixed with glutaraldehyde and osmium tetroxide and embedded in araldite. Glomerular basement membrane (GBM) thickness was measured and compared using an unpaired t-test. Angiotensin II type-1 receptor antagonism by Losartan inhibited renal growth and delayed nephron maturation. Increased immunoreactivity of TGF-β's was observed in developing nephron precursors and interstitial cells in the experimental group. Electron microscopical examination showed that thickening of the GBM was normal in the control group but an irregular thickening was seen in the experimental group (p<0.001). It was also seen that epithelial cells of developing tubules underwent apoptosis in the experimental group. Thus, renal development in rats seems to depend on an intact renin-angiotensin system. © 2005 Elsevier GmbH. All rights reserved.
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    Investigation of histopathological and cytogenetic effects of heavy metals pollution on Cyprinus carpio (Linneaus, 1758) in the Göhnarmara Lake, Turkey
    (2010) Gurcu B.; Yildiz S.; Koca Y.B.; Koca S.
    Concentration of heavy metals were measured in the surface water, sediments and three different organs of Cyprinus carpio from the Gölmarmara lake (Manisa, Turkey). Four sampling sites located at different parts of the lake were pre-defined. The accumulation of copper, zinc, cadmium, cobalt, lead, chromium, iron, mangane in gills, liver and muscle of C. carpio were determined. Also, histopathological changes in gill, liver and muscle tissue were examined at light microscopical level. The order of accumulation of heavy metals was found to be Zn>Pb>Fe>Co>Mn>Cr>Ni>Cu>Cd in water, Zn>Cr>Pb>Fe>Co>Mn>Ni>Cu>Cd in sediment, Zn>Cr>Pb>Ni>Cu>Fe>Co>Cd in muscle and Zn>Cr>Pb>Cd>Ni>Cu>Fe>Co in liver and Zn>Cr>Ni>Pb>Cu>Cd>Co>Fe in gills. As a result of histopathological examinations, a significant decrease in mean length of primary and secondary lamellae was observed. Cellular proliferation caused secondary lamellae fusion, ballooning degenerations of secondary lamellae as well as distribution of necrotic and clavate secondary lamellae. In the liver, altered staining, swollen and ruptured parenchymal cells reduce of glycogen in hepatocytes and vacuolar structure filled with cellular debris were seen. In muscle tissue, focal necrosis, cellular dissolution and a decline or loss of striatation in muscle fibres were found. The frequency of micronucleus formation did not show significant differences in fish samples caught from the Golmarmara lake. © Medwell Journals, 2010.
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    Histopathological and genotoxic effects of pollution on Anguilla anguilla in the Gediz River (Turkey)
    (2010) Yildiz S.; Gurcu B.; Basimoglu Koca Y.; Koca S.
    The aim of the present study was to determine heavy metal accumulation in water, sediment and some tissues of Anguilla anguilla along with determining histopathological and genotoxic effects of accumulation on these tissues by using light microscopy. Water, sediment and fish tissue samples taken from different sites of 3 different study areas were studied and the order of accumulation of metals was Fe>Pb>Mn>Co>Zn>Ni> Cr>Cu>Cd in water, Fe>Co>Mn>Pb>Zn>Ni>Cr>Cu>Cd in sediment and Cd>Mn>Cu>Cr>Ni>Fe>Zn>Co>Pb, Cd>Pb>Ni>Cr>Mn>Cu>Fe>Co>Zn and Cr>Ni>Zn>Cd>Pb>Mn>Cu>Fe>Co in liver, muscle and gill of fish, respectively. In histopathological studies, a decrease in the length of primary and secondary lamellae of gills, fusion in secondary lamellae, cellular proliferation, clavate lamellae formation and necrosis were observed. In liver tissue, dilation of sinusoid, increase in the number of erythrocytes, ruptured hepatocytes, decrease in glycogen accumulation and vacuolization were observed. In muscle tissue, necrosis, cellular dissolution and loss of striatation in muscle fibers were found. It was observed that pollution of water had no genotoxic effect onAnguilla anguilla. © Medwell Journals, 2010.
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    Effects of lacosamide “a novel antiepileptic drug” in the early stages of chicken embryo development
    (Springer Verlag, 2016) Mete M.; Gurcu B.; Collu F.; Unsal U.U.; Duransoy Y.K.; Tuglu M.I.; Selcuki M.
    Introduction: Antiepileptic drugs (AEDs) are teratogens and confer a risk of congenital malformation. The estimated prevalence of major congenital malformations such as cardiac defects, facial clefts, hypospadias, and neural tube defects in epileptic women is 4–10 %, which represents a two- to fourfold increase in pregnant women compared to the general population. However, there are no clear data for newer drugs. Lacosamide (LCM), a novel AED, is the first of the third-generation AEDs to be approved as adjunctive therapy for the treatment of partial-onset seizures. There are no data on the pharmacokinetics of LCM during pregnancy, and only some published data have reported on its effects during pregnancy. Methods: In this study, three different doses of LCM (0.12, 0.5, and 1.60 mg in 0.18 mL) were applied under the embryonic disks of specific pathogen-free Leghorn chicken embryos after a 30-h incubation. Incubation was continued for 80 h, at which time all embryos were evaluated macroscopically and microscopically. Results: There was growth retardation in all of the LCM-treated groups. Major malformations increased in a dose-dependent manner and were mostly observed in the supratherapeutic group. Conclusion: Based on our data, LCM may cause growth retardation or major congenital malformations. Nevertheless, more extensive investigations of its reliability are needed. © 2016, Springer-Verlag Berlin Heidelberg.
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    Neuroprotective effects of oleocanthal, a compound in virgin olive oil, in a rat model of traumatic brain injury
    (Turkish Neurosurgical Society, 2018) Mete M.; Aydemir I.; Unsal U.U.; Collu F.; Vatandas G.; Gurcu B.; Duransoy Y.K.; Taneli F.; Tuglu M.I.; Selcuki M.
    AIM: To evaluate the neuroprotective effects of deocanthal OC in a rat model of traumatic brain injury (TBI). MATERIAL and METHODS: Twenty-six adult male, Wistar albino rats were used. The rats were divided into 4 groups. Group 1 was the sham group (n = 5). Group 2 was the trauma group (n = 5) where rats were treated with 10 mg/kg saline intraperitoneally (IP) twice a day. Groups 3 and 4, rats were treated with 10 (group 3, n = 8) or 30 (group 4, n = 8) mg/kg OC IP twice a day. For each group, brain samples were collected 72 hours after injury. Brain samples and blood were evaluated with histopathological and biochemical methods. RESULTS: Histopathological evaluation revealed a significant difference between Group 2 and Group 4. Biochemical findings demonstrated that the oxidative stress index was highest in Group 2 and lowest in Group 4. CONCLUSION: OC has a protective effect on neural cells after TBI. This effect is achieved by reducing oxidative stress and apoptosis. © 2018 Turkish Neurosurgical Society.
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    Genotoxic Effect, Oxidative Stress and Cell Death due to Metronidazole Application in Gills and Liver Tissues of Rainbow Trout (Oncorhynchus mykiss)
    (University of Tehran, 2022) Gurcu B.; Koca S.; Koca Y.B.; Collu F.; Ibrahim Tuglu M.
    In this study, the purpose was to investigate the histopathological, genotoxic effect, oxidative stress and cell death due to Metronidazole (MTZ), which is a 5-nitroimidazole compound, used widely for the treatment of anaerobic organism infections in fish and humans on gill and liver tissues of Oncorhynchus mykiss. Trout fishes were exposed to 5, 10, and 20 mg/L of MTZ in the aquariums for 2, 4 and 8 days. Staining technics namely H&E, NOS immunohistochemistry, and TUNEL were performed to determine histopathological changes, oxidative damage and apoptosis. Additionally, smear preparations were also prepared from gill blood for genotoxic evaluations. The organ damage started in the 2nd day with 5 mg/L MTZ application and effects increased per duration and dose-dependent manner. It was observed that the gills had the primary and secondary lamellae lengths, with formation of clavate lamellae, fusion in secondary lamellae, separation of epithelium and aneurysm. Regional necrosis, vacuolization of hepatocytes, pycnotic nucleus, enlarged sinusoids were also determined in the liver. NOS immunoreactivity increased with the inducible immunoreactivity (iNOS) that was more prominent when compared to the endothelial immunoreactivity (eNOS). Apoptotic immunoreactivity was higher in the 10 mg 8th day experimental group at liver and gills, and was lower 20 mg 8th day experimental group. When the gills and liver compared with each other, in all doses, immunoreactivity was lower in gills, compared with liver. Genotoxic examinations showed that both number of micro nucleated erythrocytes and nuclei abnormalities were higher in MTZ-treated groups. © 2022 University of Tehran. All rights reserved.
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    Cytotoxic Effects of Hypericum Perforatum on Glioblastoma Cells by Inducing Oxidative Stress, Autophagy and Apoptosis
    (Turkish Neurosurgical Society, 2025) Mete M.; Unsal U.U.; Collu F.; Aydemir I.; Kocamaz E.; Barutcuoglu M.; Gurcu B.; Karakayali M.; Tuglu M.I.
    AIM: To identify the autophagy mechanism T98 glioma cells. MATERIAL and METHODS: Three groups were created with T98 human glioblastoma cells; Group 1: T98 glioma cells without treatment (Control group). Group 2: T98 glioma cells treated with 3 µl/ml JWO. Group 3: T98 glioma cells treated with 6 µl/ml JWO. The cell proliferation, oxidative stress, types of cell death were studied at IC50 dose of JWO. RESULTS: The proliferation of glioma cells was inhibited in 5.296 µl/ml dose. JWO induced apoptosis in T98 glioma cells in comparison with the control and there was statistically significant difference (p<0.001). Apoptosis was analyzed via TUNEL method and results were checked by flow cytometry. We also investigated the effects of JWO on autophagy in T98 glioma cells by immunostaining LC3-II and MDC fluorescent stainings. The differences between JWO treated and control group were notably significant (p<0.001). The immunofluorescence staining resultsof LC3-II was confirmed by Western blotting analysis. CONCLUSION: JWO seems to be an effective treatment agent for glioblastoma. Not only does it induce apoptosis via oxidative stress but also affects the autophagy. The use of JWO in combination with other treatment options may increase the efficacy of treatment. © (2025), (Turkish Neurosurgical Society). All rights reserved.