Browsing by Author "Gurgul, S"

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    The preventive effect of N-acetylcysteine on radiation-induced dermatitis in a rat model
    Demirel, C; Kilciksiz, S; Evirgen-Ayhan, S; Gurgul, S; Erdal, N
    Purpose: We investigated the potential radioprotective effects of N-acetylcysteine (NAG) comparing its effects with that of amifostine (WR-2721), as a representative of clinically used radioprotecor, in ameliorating skin injury from irradiation in rats (single dose, 18 Gy to the left hind legs of the rats). Methods: The rats (n=28) were divided randomly and equally into 4 groups: Control (C), Radiation (R), R+WR-2721 (received irradiation and 200 mg/kg of WR-2721) and R+NAC (received irradiation and 1000 mg/kg of NAG). Acute skin reactions were assessed every 3 days by a radiation oncologist and a biophysicist. Light microscopic findings were assessed by an expert pathologist. Results: Clinically and histopathologically, irradiation increased dermatitis when compared with the control group (p<0.05). The severity of radiodermatitis of the rats in the R+NAC and R+WR-2721 groups was significantly lower than in the R group (p<0.05). The protective effects of NAC and WR-2721 on irradiation increased dermatitis were clinically and histopathologically similar (p>0.05). Conclusion: The study gives clues about the beneficial effects of NAC against radiation-induced dermatitis.
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    N-acetylcysteine ameliorates nitrosative stress on radiation-inducible damage in rat liver
    Kilciksiz, S; Demirel, C; Ayhan, SE; Erdal, N; Gurgul, S; Tamer, L; Ayaz, L
    Purpose: The present study was designed to investigate the potential radioprotective effects of N-acetylcysteine (NAC) on radiation-induced nitrosative stress caused by gamma irradiation (single dose, 6 Gy) in rat liver Methods: The rats (n=40) were divided randomly and equally into 4 groups: Control (C), Radiation (R), R+NAC (received irradiation and 1,000 mg/kg of NAC) and R+WR-2721 (received irradiation and 200 mg/kg of WR-2721). Liver tissue of each animal was harvested and utilized for 3-nitrotyrosine (3-NT) detection using high-performance liquid chromatography-ultraviolet (HPLC-UV) system. Results: In the R rats, 3-NT levels significantly increased when compared to those of the C rats (p<0.05). There were no significant differences in the 3-NT levels among R+NAC and R+WR-2721 rats. Histologically examined liver tissue samples showed no obvious differences. Conclusion: The present study suggests that irradiation has a negative effect on the cellular proteins by enhancing 3-NT formation. The prophylactic use of NAC seems to reduce the nitrosative damage during radiotherapy.