Browsing by Author "Hafize DİLEK TEPE"

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    Determination of Phenolic Compounds, Organic Volatile Molecules and Anti-Cancer Properties in Inula Viscosa L., Viscum Album L. and Raphanus Sativus L.
    (2021) Hafize DİLEK TEPE; Aslı UĞURLU BAYARSLAN; İdris YAZGAN
    The plants elecampane (Inula Viscosa L.), mistel (Viscum album L.) and black radish seed (Raphanus Sativus L.) have been used in the treatment of common diseases worldwide as part of traditional medicine for many years. Especially in Turkey, elecampane plant is commonly used as remedy of cancer. In this study, phytochemical components of these three plants were analyzed using liquid chromatography-mass spectrometer/mass spectrometer and gas chromatography-mass spectrometer techniques. Antioxidant activity of the characterized extracts were evaluated using DPPH assay, followed by biological properties were studied using MDA-MB-231 breast cancer line. Differences in the chemical compositions of the extracts resulted in alteration in antioxidant potentials, where elecampane gave the highest antioxidant activity while black radish seed extracts did not provide any meaningful results within the test period. Cytotoxicity studies showed that chemical composition is of the most prominent factor that defined the IC50 value of each extract, where pro-oxidant and antioxidant affects were observed in relation to presence of flavonoids. Mistel extract was further tested for wound healing and apoptosis tests, and the extract was obtained as a trigger for both apoptosis and wound-healing. The findings can be a basis for refinement as fractionation of the mistel and elecampane extracts so as to obtain the best mixture that can serve as strong anticancer agent mixture.
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    Comparison of Microwave, Ultrasonic Bath and Homogenizer Extraction Methods on the Bioactive Molecules Content of Green Tea (Camellia Sinensis) Plant.
    (2023) Hafize DİLEK TEPE; Fatma DOYUK
    In this study, the effects of three different extraction methods on the antioxidant capacity, phenolic component, volatile organic molecule and amino acid contents of green tea (Camellia sinensis) leaves were compared. Microwave digestion assisted extraction (MDAE), ultrasonic bath assisted extraction (UBAE) and homogenizer assisted extraction (HAE) methods were used as extraction methods. When the MDAE, UBAE and HAE extraction methods were compared according to the ferric reducing antioxidant power (FRAP) antioxidant activity results, 51.95, 50.9 and 13.8 TE/g dry weight (DW) results were obtained in green tea plant, respectively. DPPH activity in green tea was found to be 94.65, 69.7 and 36.35 TE/g dry weight in microwave extraction, ultrasonic bath and homogenizer extractions, respectively. Analysis in green tea leaves as a result of gas chromatography-mass spectrometry (GC-MS), caffeine, phytol, palmitic acid, beta.-monoglyceride, 13-docosenamide, (z)- and phytol were identified in all three extractions. Fourteen amino acid types were determined in green tea leaves by liquid chromatography-diode array detection (HPLC-DAD). Especially aspargine, glutamine, alanine, serine and L-theanine were determined in very high amounts. When the analysis data were compared, it was determined that the best results were in MDAE>UBAE>HAE, respectively