Browsing by Author "Hanoğlu D.Y."

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    The effect of Colchicum pusillum in human colon cancer cells via Wnt/β-catenin pathway
    (Elsevier B.V., 2019) Becer E.; Hanoğlu D.Y.; Kabadayı H.; Hanoğlu A.; Vatansever S.; Yavuz D.; Meriçli F.; Meriçli A.H.
    Objective: Colchicum pusillum belongs to the family Colchicaceae that particularly rich in tropolonic alkaloids. The aim of this study was to investigate the cytotoxicity and in vitro anticancer activity of Colchicum pusillum ethanolic extract on Colo-320 primer and Colo-741 metastatic colon adenocarcinoma cell lines. Materials and methods: Colchicum pusillum was collected and extracted with ethanol. Different concentrations of Colchicum pusillum extract were incubated for 24 h and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assays. Anticancer and antiproliferative activities of Colchicum pusillum were investigated by immunocytochemistry using antibodies directed against to β-catenin, Ki-67, LGR-5 Ki-67, DKK1, Frizzled-4, Wnt4, Wnt7a and caspase3 in Colo-741 cells. Results: All concentrations of Colchicum pusillum extract had toxic effect in Colo-320 cells. Because of this, we used Colchicum pusillum extract at 20 μg/ml for evaluate anticancer activities only in Colo-741 cells. As a result of immunohistochemical staining, β-catenin, LGR-5 and caspase-3 immunoreactivities were significantly increased while Wnt7a immunostaining intensity was decreased in Colo-741 cells. Conclusion We conclude that Colchicum pusillum extract increased β-catenin and LGR-5 via Wnt/β-catenin pathway in colon cancer cells. Interestingly, it decreased other signaling molecule, Wnt7a which is assumed to play protective role during carcinogenesis. Also, it increased significantly caspase-3 immunoreactivity showing that apoptotic pathways were triggered. © 2018
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    Composition and antibacterial, anti-inflammatory, antioxidant, and anticancer activities of Rosmarinus officinalis L. essential oil
    (Elsevier B.V., 2023) Becer E.; Altundağ E.M.; Güran M.; Seda Vatansever H.; Ustürk S.; Hanoğlu D.Y.; Hüsnü Can Başer K.
    Rosemary is a culinary herb that is widely used in traditional medicine for its nutritional value and pharmacological properties. The aim of this study was to investigate the composition, in vitro anticancer, antioxidant, and antibacterial activities of rosemary essential oil. Rosemary essential oil components were simultaneously analysed by GC/MS and GC/FID techniques. Different concentrations of rosemary essential oil were incubated for 24 and 48 h with HepG2 and EV304 cells. Cell viability was measured by MTT assay. Anticancer activities of rosemary essential oil were investigated by immunocytochemistry using antibodies directed against Ki-67, β-catenin, c-myc, Oct-3/4, and IL-8. Anti-inflammatory and antioxidant activity were evaluated with protein denaturation and DPPH assays, respectively. The anti-bacterial effect of rosemary essential oil was analysed by Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae with the broth microdilution and agar zone diffusion methods. Camphor (15.1%), verbenone (14.3%), α-pinene (13.6%), 1,8-cineole (11.8%), and borneol (7.9%) were determined as the major compounds of rosemary essential oil. The activities of the oil were found to be 508.7 µg/ml and 525.7 µg/ml against HepG2 and ECV304 cells, respectively. Ki-67, β-catenin, c-myc, Oct-3/4, and IL-8 immunoreactivities were significantly reduced in rosemary essential oil-treated HepG2 cells. Ki-67 and β-catenin immunoreactivities were significantly decreased only in rosemary essential oil-treated ECV304 cells. Also, the essential oil showed antioxidant and free radical scavenging activities. Rosemary essential oil showed effective antibacterial activity on E. coli and K. pneumoniae. Thus, rosemary essential oil could be a potential candidate as a therapeutic agent in cancer treatment. © 2023 SAAB