Browsing by Author "Hekimgil M."

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    Histopathological and immunohistochemical clues in the differential diagnosis of benign and malignant focal lymphocytic aggregates in paraffin sections of the bone marrow biopsies
    (1998) Isisag A.; Hekimgil M.; Soydan S.; Ayhan S.; Turkdogan P.
    One of the problems in evaluating bone marrow trephine biopsies is to determine whether a focal lymphocytic aggregate is benign or not. To assess the value of anti-bcl-2 monoclonal antibody on the differential diagnosis, bone marrow biopsies with focal lymphocytic aggregates were evaluated by conventional histopathological criteria and an immunohistochemical study using anti-bcl-2 monoclonal antibody was performed. Of the 27 bone marrow trephine biopsies from patients with lymphoproliferative disorders malignant aggregates were found in 14. The remaining 13 contained benign aggregates according to the conventional criteria and in none of these cases malignancy was confirmed. Conventional criteria were tested by comparing the histopatological characteristics of benign and malignant aggregates. The trephine biopsies of all 27 cases were immunostained with anti-bcl-2 antibody. A moderate to intense nuclear staining in the mononuclear calls of the aggregates was observed in 12 of 14 malignant cases. None of the cases diagnosed as benign stained with the anti-bcl-2 monoclonal antibody. It is concluded that positive immunostaining with this antibody strongly suggests that a focal lymphocytic aggregate is malignant.
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    Systemic mastocytosis presenting with a prominent B lymphocyte proliferation in the bone marrow and extensive fibrosis of the spleen
    (IOS Press, 2007) Neşe N.; Çaǧirgan S.; Ertan Y.; Sönmez A.; Soydan S.; Hekimgil M.
    Systemic mastocytosis is a disease characterized by multifocal mast cell proliferation in the bone marrow or other extracutaneous organs. Because of loosely scattered and hypo-/agranular mast cells, the diagnosis is sometimes very difficult. In the bone marrow, mast cell infiltration may be associated with prominent lymphoid infiltration leading to a misdiagnosis of a low grade non-Hodgkin lymphoma. A 49-year-old woman presented with right arm and leg pain, psychiatric symptoms, and diarrhea for four years. Physical examination and laboratory investigation revealed hepatosplenomegaly, anemia, mild thrombocytosis, mild leucocytosis and lymphocytosis. In the bone marrow biopsy, there was a prominent B lymphocyte proliferation reminiscent of a low grade non-Hodgkin lymphoma/leukemia and there were some spindle cells aggregates in paratrabecular location. The consecutive bone marrow biopsies were similar to the first. The subsequent splenectomy specimen exhibited striking fibrosis. In the lymph node sections, there was marginal zone hyperplasia. Multifocal accumulations of mast cells were strongly positive with mast cell tryptase and CD117 on immunohistochemical staining, though no metachromasia was identified in Giemsa and Toluidine Blue stained aspirates and tissue sections, probably due to hypo-/agranulation of mast cells. The case was presented to emphasize the importance of the antibody to mast cell tryptase in the diagnosis of mastocytosis and to discuss problems of differential diagnosis of systemic mastocytosis. © 2007 - IOS Press and the authors. All rights reserved.
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    Histopathological and immunohistochemical features of 32 cases of splenic B-cell lymphoma and leukemia
    (Turkiye Klinikleri, 2009) Kaçar Döger F.; Hekimgil M.; Ertan Y.; Sarsik B.; Soydan S.; Neşe N.
    Objective: Leukemias and non-Hodgkin lymphomas commonly involve the spleen or originate primarily in the spleen and then spread to other sites. Material and Methods: In this retrospective study, we examined the histopathological and immunohistochemical characteristics of 32 cases of primary or secondary splenic B-cell lymphoma and leukemia, in which the diagnosis was established according to the World Health Organization (WHO) classification. The immunohistochemical panel included ALK-1, BCL-2, BCL-6, CD3, CD5, CD10, CD20, CD21, CD23, CD30, CD43, cyclin D1, Ki-67, and TRAP. Results: There was no other nodal or extranodal disease involvement in the majority of patients diagnosed with lymphoma at the time of presentation, while cases of leukemia had undergone splenectomy for palliative purposes. The diagnoses were as follows: 11 cases of hairy cell leukemia (HCL, 34.4%), 8 cases of splenic marginal zone lymphoma (SMZL, 25%), 8 cases of diffuse large B-cell lymphoma (DLBCL, 25%) including 1 T-cell-rich B-cell lymphoma (TCRBCL), 4 cases of mantle cell lymphoma (MCL, 12.5%), and 1 prolymphocytic leukemia (PLL, 3.1%). Conclusion: Overall assessment of spleen, liver, bone marrow, and lymph node examinations and a detailed correlation of the histopathological and immunohistochemical features with the clinical findings are very helpful and usually lead to the final diagnosis in most cases of primary or secondary splenic B-cell lymphoma and leukemia. Copyright © 2009 by Türkiye Klinikleri.
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    Sclerosing extramedullary hematopoietic tumor mimicking intra-abdominal sarcoma
    (Seoul National University, 2015) Karaarslan S.; Nese N.; Oncel G.; Ozsan N.; Akalin T.; Kaplan H.; Buyukkececi F.; Hekimgil M.
    [No abstract available]