Browsing by Author "Kabaroglu, C"

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    Oxidative stress impairs endothelial nitric oxide levels in Behcets' disease
    Onur, E; Kabaroglu, C; Inanir, I; Var, A; Guvenc, Y; Gunay, O; Gunduz, K
    Background: Behcet's disease (BD) is an inflammatory vasculitis. Endogenous nitric oxide (NO), produced by endothelial cells, has pleiotropic effects such as vasodilatator, antiplatelet, antiproliferative. Reactive oxygen species (ROS) are produced at sites of endothelial inflammation. ROS target polyunsaturated lipids, which results in malondialdehyde (MDA) production. Objective: The aim was to investigate the oxidative stress in BD patients by measuring MDA and total antioxidant status (TAS) levels and to establish a possible relationship with respect to NO levels regarding disease activity. Materials and methods: 55 BD patients (30 active/25 inactive) and 20 healthy volunteers were included in the study. Blood samples were drawn following an overnight fasting. TAS and MDA levels were determined spectrophotometrically. Serum nitrite (NO2-) and nitrate (NO3-) levels were measured to estimate NO production. Data were expressed as mean +/- SD. Results: TAS levels were significantly lower in BD patients than the controls (1.19 +/- 0.34 vs. 3.29 +/- 0.89 mmol/L). In the active BD group, MDA levels (0.36 +/- 0.19 nmol/mL) were significantly higher than both the inactive BD group (0.25 +/- 0.18 nmol/mL) and controls (0.18 +/- 0.41 nmol/mL). NO levels were significantly lower in the active group compared to the inactive group (18.0 +/- 2.80 vs. 19.40 +/- 2.70 mu mol/L). MDA levels correlated negatively with NO levels in the active group. Conclusion: Decreased NO levels mediated by increased oxidative stress significantly contribute to endothelial dysfunction observed in BD.
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    Inflammatory marker levels in obese adolescents with glucose intolerance: Increased chitotriosidase activity
    Kabaroglu, C; Onur, E; Barutçuoglu, B; Özhan, B; Erdinç, S; Var, A; Bayindir, O; Ersoy, B
    Objectives: Existance of low grade persistent inflammation in obese children may increase the risk of metabolic and cardiovascular events. The aim was to determine whether glucose intolerance has an influence on inflammatory markers in obese adolescents. Designs and methods: 45 obese adolescents (mean BMI: 30.34 +/- 5.42 kg/m(2)) were grouped as normal or impaired glucose tolerance. IL-6 and CRP levels were analyzed by commercially available kits. Chitotriosidase activity was measured by a fluorescence method and neopterin levels were determined by ELISA. Data were expressed as mean +/- SD. Results: IL-6 and CRP levels were similar in the two groups. Serum neopterin levels were not different between the groups. The chitotriosidase activity was significantly higher in the IGT group than NGT (124.33 +/- 51.97 mu mol/L/h vs 84.50 +/- 53.99 mu mol/L/h, p = 0.04). Conclusion: Serum chitotriosidase activity is increased in obese adolescents with impaired glucose tolerance. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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    Elevated glucose level at 30 minutes during an oral glucose tolerance test in obese adolescents: a new disorder of glucose tolerance
    Kabaroglu, C; Ersoy, B; Onur, E; Özhan, B; Erdin, S; Var, A; Bayindir, O; Dinç, G
    We observed glucose levels >140 mg/dL measured at 30 minutes (min) during an oral glucose tolerance test (OGTT) in some obese patients. We aimed to investigate the significance of this finding by comparing lipid profiles, insulin resistance indices, and systemic inflammatory mediators between obese adolescents with normal glucose tolerance (NGT), impaired glucose tolerance (IGT), and elevated glucose levels at 30 min. The study involved 80 obese (body mass index >95th percentile for age and sex) adolescents (48 female, 32 male) between 11 and 16 years of age. Depending on OGTT results, patients were divided into NGT and IGT groups. The third group was recruited from the NOT group as having glucose levels > 140 mg/dL at 30 minutes. Lipid profiles, [interleukin-6 (IL-6)], neopterin, and lipoprotein associated phospholipase A(2) (Lp-PLA(2))] were assessed. Neopterin and Lp-PLA(2) levels were significantly higher in obese adolescents with elevated glucose levels at 30 min. compared with those in both NGT and IGT groups (p=0.013, and 0.004, respectively). In these adolescents, IL-6 levels were significantly higher only than the NOT group (p=0.01). In logistic regression analysis, IL-6, neopterin and Lp-PLA(2) levels were detected to be related to high blood glucose levels at 30 min (OR 1.11, p = 0.01; OR 9.03, p=0.013; OR 1.01, p=0.004 respectively). Obese adolescents with elevated glucose levels at 30 min. demonstrated higher inflammatory mediators levels, which were atherosclerotic indicators, than obese adolescents with NGT and IGT. These results suggest that glucose levels >140 mg/dL measured at 30 min during an OGTT may be a new disorder of glucose tolerance in obesity.
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    Treatment and prophylaxis with sucralfate ameliorates hypoxia/reoxygenation-induced intestinal injury in pup rats
    Sencan, AB; Sencan, A; Aktas, S; Habif, S; Kabaroglu, C; Parildar, Z; Karaca, I
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    Metoclopramide use to induce lactation can alter DRD2 and BDNF in the prefrontal cortex of offspring
    Gürgen, SG; Yazici, GN; Gözükara, C; Kabaroglu, C; Onur, E
    Metoclopramide, used as an anti-emetic drug in clinical practice, has recently also begun being used to establish hyperprolactinemic effects in breastfeeding. The purpose of this study was to investigate the potential side-effects of metoclopramide applied in the lactation period in the central nervous system of offspring rats. Eighteen female albino Wistar rats that had just given birth were divided into three groups together with their pups, healthy controls, low-dose metoclopramide (10 mg/kg, twice per day i.p.) and a high-dose metoclopramide group (45 mg/kg, twice per day i.p.). Brain tissues from six pups from each mother were harvested at the end of the 21st day. Immunohistochemical and ELISA techniques were performed using dopamine D2 receptor (DRD2), brain derived neurotrophic factor (BDNF) and neural growth factor (NGF), markers of extrapyramidal reaction in the brain, as signal molecules. Based on biochemical levels and immunohistochemical results, DRD2 expression decreased only in the external pyramidal layer neurons in the high-dose offspring group. Strong BDNF reaction was determined in pyramidal neurons in all layers in the control offspring group, and decreased reaction was observed in the high- and low-dose groups. No significant difference was observed in NGF expression between the three groups. Since high-dose metoclopramide caused a decrease in DRD2 expression in the external pyramidal layer in the prefrontal cortex, and since both high and low doses reduced BDNF expression, care needs to be taken with the use of metoclopramide in the lactation period due to the possibility of extrapyramidal reactions in offsprings.
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    The beneficial effects of physical exercise on antioxidant status in asthmatic children
    Onur, E; Kabaroglu, C; Günay, Ö; Var, A; Yilmaz, Ö; Dündar, P; Tikiz, C; Güvenç, Y; Yüksel, H
    Background: The pathogenesis of asthma involves both airway inflammation and an oxidant/antioxidant imbalance. It is demonstrated in asthmatic adults that exercise programmes improve lung function, a mechanism yet to be elucidated. The purpose of this study was to investigate the possible beneficial effects of physical exercise on antioxidant status in asthmatic children which may lead to ameliorated lung function. Methods: The study enrolled thirteen control and thirty asthmatic children. The asthmatic group was subdivided into two: the first group receiving only pharmacological treatment (n = 15) and the second receiving pharmacological treatment with exercise programme (n = 15) for 8 weeks. Blood samples were drawn from the subjects before and after treatment periods. As oxidant stress markers blood levels of malondialdehyde (MDA) and total nitric oxide (NO), and as antioxidant status, glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) enzyme activities were assessed. Results: Before any treatment was initiated, MDA and NO levels in the asthmatic group were significantly higher than the controls (3.40 +/- 0.96 nmol/ml vs 2.46 +/- 0.58 nmol/nnl, and 12.53 +/- 2.10 vs 9.40 +/- 1.39 micromol/L, respectively). Both SOD (p=0.0001) and GSH-Px (p=0.023) activities were significantly lower in the asthmatic group. Pharmacological treatment and exercise programme together significantly improved lung performance and decreased the levels of oxidant stress markers, in concordance with a significantly increase in antioxidant enzyme activity measures when compared to the pharmacological treatment. Conclusion: Structured exercise programme in asthmatic children resulted in better lung function, which may be attributed to its effect on antioxidant status. (C) 2010 SEICAP. Published by Elsevier Espana, S.L. All rights reserved.