Browsing by Author "Kahyaoglu, F"

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    Investigating the Effect of Rosuvastatin, Paracetamol and Co- administration of Rosuvastatin and Paracetamol on Ocular Tissue
    Ünsal, AIA; Kahyaoglu, F; Özoran, Y; Gökçimen, A; Demirci, B
    Objective: Statins and Paracetamol are two drugs that have a high prescription rate all over the world. Possible side effects can easily be augmented because they use the same cytochrome oxidase enzymes in liver. This study aimed to investigate the effect single or combined administration of these drugs on ocular tissues. Materials and Methods: Twenty-eight 12- to 15-month-old rats were divided in four groups: Control, Rosuvastatin (10 mg/kg/day for 7 times a week), Paracetamol (50 mg/kg/day for 5 times a week) and Rosuvastatin (10 mg/kg/day for 7 times a week) + Paracetamol (50 mg/kg/day for 5 times a week) for 8 weeks. At the end of study, intraocular pressure (IOP) was measured and ocular tissues were obtained for histopathological evaluation under anaesthesia with Ketamine and Xylasine (50 mg/kg and 5 mg/kg, respectively). Results: Rosuvastatin showed an IOP dropping effect and paracetamol did not prevent it. Histopathological evaluation mainly revealed retinal nerve fibre layer degeneration. Additionally, different pathological alterations such as corneal oedema and polypoid proliferation were observed in all the treated groups, although they were rare. Conclusion: The IOP dropping effect of rosuvastatin shows that it is safe in glaucoma patients, but this beneficial effect was not observed with Paracetamol. Retinal nerve fibre layer degeneration with both drugs might be one of the reasons for visual disturbances in real life conditions.
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    The effect of Paracetamol exposure on hepatic and renal tissues during statin usage
    Aksun, S; Gökcimen, A; Kahyaoglu, F; Demirci, B
    Objective: Statins and Paracetamol have widespread use in clinic and both drugs possess similar side effects; therefore, we investigated if drug-interaction occurs when the combination of these two drugs is used during therapy. Materials and methods: A total of 32 (12-15 months old) grown-up male rats were divided into four groups: Control group, RSV group (10 mg/kg Rosuvastatin/daily), APAP group (50 mg/kg Paracetamol/5 days/weekly), RSV + APAP (10 mg/kg Rosuvastatin/daily + 50 mg/kg Paracetamol/5 days/weekly). At the end of 8 weeks of chronic treatment, the blood and tissue samples were taken under the Ketamine and Xylasine anesthesia (50 mg/kg and 5 mg/kg, respectively). Results: In the liver, sinusoidal dilatations, pyknotic nuclei and hemorrhagic foci are more frequently seen in the group receiving combination therapy; although serum liver functions among groups were not significantly different. Kidney histopathologic alterations in APAP and RSV + APAP groups were found more distinct than in RSV alone group. Inducible nitric oxide synthase activity was highly increased with combination therapy in liver and kidney tissues. Conclusion: RSV-Paracetamol interaction may occur as an important drug interaction histopathologically even before it is manifested biochemically in the clinic.
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    Comparison of Three Different Rotavirus Antigen Tests for Rotavirus Detection in Fecal Samples: A Retrospective Analysis
    Kirdar, S; Erol, N; Kahyaoglu, F; Yazici, V; Örün, H; Altindis, M
    Objective: Direct antigen tests are the most commonly used methods in most laboratories to detect rotavirus rapidly in stool samples. This study aimed to evaluate the performance of three commercially available test methods for detecting rotaviruses in fecal specimens and compare the results with those of the reverse transcription-polymerase chain reaction (RT-PCR), which is considered a gold standard test.Materials and Methods: The presence of rotavirus antigens in stool samples was investigated by an enzyme-linked immunosorbent assay (ELISA), an immunochromatographic test (ICT), and a latex agglutination test (LAT), which were commercially available. The results of these tests were compared with those of a multiplex RT-PCR as a reference test. Sensitivity, specificity, and positive and negative predictive values were calculated, and agreement with RT-PCR was evaluated by Cohen's kappa test.Results: A total of 85 patients (51.8% male and 48.2% female, aged 0-32 years) were included in this study. The sensitivities of the ICT, LAT, and ELISA tests were 78.6%, 78.6%, and 96.4%, respectively; the specificities of the tests were 69.0%, 72.4%, and 69.0%, respectively. According to kappa tests, moderate agreement was found between RT-PCR and ICT (Kappa=0.464, p<0.001); moderate agreement was found between RT-PCR and LAT (Kappa=0.493, p<0.001); substantial agreement was found between RT-PCR and ELISA (Kappa=0.694, p<0.001). The ELISA test showed the highest sensitivity and a high level of agreement with RT-PCR.Conclusion: ICT and LAT are quick and practical tests for rotavirus detection. However, in this study, it was seen that they were not superior to the ELISA test in terms of accuracy of diagnosis.