Browsing by Author "Kanik E.T."

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    E-cadherin as an epithelial barrier protein in exhaled breath condensate
    (Institute of Physics Publishing, 2014) Yuksel H.; Turkeli A.; Taneli F.; Horasan G.D.; Kanik E.T.; Kizilkaya M.; Gozukara C.; Yilmaz O.
    Airway epithelium plays an important role as a physical barrier and a modulator of allergic response. Junctions between cells provide epithelial integrity and barrier function. The aim of this study was to investigate the influence of atopy on airway epithelial integrity in asthma and to measure E-cadherin levels in exhaled breath condensate as an indicator epithelial damage. A total of 74 patients with asthma (35 atopic and 39 non-atopic) and 39 healthy children were enrolled in this case-control study. Sociodemographic characteristics and asthma severity parameters in the last three-month period were recorded and pulmonary function tests were performed. Blood samples were obtained to measure serum immunoglobulin E (IgE) levels and peripheral blood eosinophil count, and exhaled breath condensate (EBC) was obtained to measure E-cadherin. EBC E-cadherin levels were significantly lower in the asthmatics when compared to non-atopic controls (0.109 (0.076) versus 0.191 (0.184) ng mL-1 respectively, p = 0.01). Atopic and non-atopic asthmatic groups had lower EBC E-cadherin levels compared to the control group. (0.112 (0.060) ng ml-1, 0.106 (0.089) ng ml-1 and 0.191 (0.184) ng ml-1, p = 0.02 and p < 0.01 respectively). However, EBC E-cadherin levels were not different between atopic and non-atopic asthmatics. The results of our study support the role of E-cadherin in the pathogenesis of asthma. However, the absence of difference in E-cadherin levels between atopic and non-atopic asthmatics suggests that allergic sensitization is not the primary factor for development of epithelial barrier dysfunction in asthma. © 2014 IOP Publishing Ltd.
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    IL-5, IL-8 and MMP -9 levels in exhaled breath condensate of atopic and nonatopic asthmatic children
    (W.B. Saunders Ltd, 2015) Turkeli A.; Yilmaz O.; Taneli F.; Horasan G.D.; Kanik E.T.; Kizilkaya M.; Gozukara C.; Yuksel H.
    Rationale Asthma is a heterogeneous disease, and a great majority of pediatric patients with asthma demonstrate atopic characteristics and develop a Th2 type cytokine response. Nonatopic asthma, on the other hand, is seen more rarely. Methods In this study, levels of IL-5, IL-8 and MMP-9 were measured in exhaled breath condensate (EBC) of the subjects to demonstrate the extent of tissue damage as well as eosinophilic and neutrophilic inflammation in children with atopic and nonatopic asthma. A total of 37 children with atopic asthma and 37 children with nonatopic asthma were enrolled in the study. Patients who exhibited protease positive aeroallergen (House dust mite, mould mix, olea, grass mix) sensitivity in allergen skin prick test were included in the atopic asthma group. To evaluate the EBC, the fluid content of the breath was collected by having the patients exhale into an EBC device, after which the IL-5, IL-8 and MMP-9 levels were assayed using the ELISA method. Results The atopic asthmatics exhibited significantly higher IL-5 levels in their EBC samples than the nonatopic asthmatics (0.271 [0.198-0.489] pg/ml and 0.198 [0.125-0.344] pg/ml, respectively, p = 0.04), while no significant differences were observed in the levels of IL-8 and MMP-9 in the EBC samples of the atopic and nonatopic asthmatics. Conclusions IL-5 levels, as a marker of eosinophilic inflammation, were demonstrated to be higher in the children with atopic asthma when compared to those with nonatopic asthma in EBC. The fact that no significant difference was apparent in the IL-8 levels between the groups suggests that it is the severity of the disease rather than the atopic state that plays an important role in IL-8 levels. Since no difference was recorded between the groups in terms of MMP-9 levels, lung damage in asthma sufferers seems to develop independent of atopia. © 2015 Elsevier Ltd.
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    The effect of standardized training in nebulizer use on disease control in asthmatic or wheezing children; [Astimli ve hişiltili çocuklarda nebülizer kullanimi konusunda verilen standart eǧitimin hastalik kontrolüne etkisi]
    (Cocuk Sagligi ve Hastaliklan Dergisi, 2015) Kanik E.T.; Yilmaz Ö.; Türkeli A.; Yüksel H.
    Inhalation therapy is administered to asthmatic patients in order to control symptoms and attacks by relieving airway obstruction. The effectiveness of this treatment depends on the correct use of inhalation devices as much as on pharmacological agents. Many asthmatic children receive no standard training in device use during intensive clinic observation, or else they are unable to apply the verbal instructions they receive. The purpose of this study was to evaluate the effect of standardized visual practical training on asthma control. Sixty children aged 1-15 who were under observation for infant wheezing or asthma and using nebulizers were included in the study. At the beginning of the study, patient's and parents' ages, occupation, education level, age at diagnosis, symptoms over the previous three months and disease severity were recorded. The child or mother's nebulizer use skills were assessed on a scale before and after training. In addition, asthma control levels were determined at the start of the study and one month after training using a symptom score and asthma control questionnaire (ACQ). Seven patients (11.7%) were being monitored for asthma and 53 (88.3%) for infant wheezing. The nebulizer use score was 5.4 before training and rose to 7.8 afterward (p<0.001). An increase was observed in the number of correct admininstrations in all six steps assessing device use skills after training. The asthma symptom score was 7.54 before training and 0.78 one month after training (p<0.001). A significant decrease was observed in the ACQ results after instruction in nebulizer device usage (2.1 and 0.1, respectively; p<0.001). Our study revealed that there were errors of device usage in children using nebulizers and diagnosed with asthma or infant wheezing, but that these could be overcome through standardized practical training. In addition, a significant improvement was achieved in respiratory symptom scores and asthma control with training in device usage.
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    Vascular endothelial growth factor antagonism restores epithelial barrier dysfunction via affecting zonula occludens proteins
    (Spandidos Publications, 2015) Yuksel H.; Yilmaz O.; Karaman M.; Firinci F.; Turkeli A.; Kanik E.T.; Inan S.
    Epithelial barrier dysfunction is important in the pathogenesis of asthma and allergic responses, and is therefore a therapeutic target. The aim of the present study was to investigate the effects of dexamethasone, a classic therapeutic agent, an anti-tumor necrosis factor agent (etanercept), which is used to treat difficult cases of asthma, and an anti-vascular endothelial growth factor (VEGF) agent (bevacizumab), which is an angiogenesis inhibitor, on zonula occludens (ZO) proteins in an experimental asthma model. The experimental model of asthma was developed using intraperitoneal (IP) and inhaled administration of ovalbumin in 38 BALB/c mice, which were divided into four groups. The control group (n=6) did not receive any treatment, while the four remaining groups (n=8 per group) received an IP injection of saline, etanercept, bevacizumab or dexamethasone, respectively. Occludin, claudin and junctional adhesion molecule (JAM) were immunohistochemically stained in the left middle lobe samples using an indirect avidin-peroxidase method, after which the staining was semiquantified with H-scores. Statistically significant differences were observed in the occludin, claudin and JAM H-scores among the four groups (P<0.001). In the untreated asthma, etanercept, bevacizumab and dexamethasone groups, the median H-scores for occludin were 93, 177, 280 and 198, respectively, while the H-scores for claudin were 82, 193.5, 274 and 202.5, respectively, and the median H-scores for JAM were 130, 210, 288 and 210, respectively. Pairwise comparisons revealed that all three ZO protein H-scores were significantly lower in the saline group when compared with each treatment group. However, the H-scores of the ZO proteins were not significantly different between the etanercept and dexamethasone groups. Furthermore, the bevacizumab group exhibited higher H-scores for all the proteins compared with the dexamethasone group. Therefore, antagonism of VEGF with bevacizumab restores the epithelial barrier to a greater extent when compared with dexamethasone treatment. This result may be promising for the development of novel therapeutic agents. © 2015, Spandidos Publications. All rights reserved.
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    Low Mononuclear Cell IL-18 and IL-27 Response in Children: Susceptibility to Tuberculosis Infection after Contact
    (Georg Thieme Verlag, 2019) Karabacak H.A.; Yilmaz O.; Tuglu I.; Taneli F.; Surucuoglu S.; Kanik E.T.; Ozkutuk N.; Gozukara C.; Ozkut M.M.; Turkeli A.; Yuksel H.
    Background Identification of the immune response against tuberculosis is vital to develop new diagnostic and therapeutic modalities. The objective of this study was to determine IL (interleukin)-18 and IL-27 responses of peripheral blood mononuclear cells to early secreted antigen (ESAT-6) and culture filtrate protein-10 (CFP-10) stimulation in children with a (+) or (-) tuberculin skin test (TST) with in-house tuberculosis contact. Methods We enrolled 40 children aged 1 to 5 years who had an in-house contact with a tuberculous adult. Blood samples were obtained from all children for QuantiFERON tuberculosis (TB) gold in tube (QFT-GIT), and peripheral blood mononuclear blood cell culture tests. The subjects were grouped as TST (-) QFT-GIT (-), TST (+) QFT-GIT (-), and TST (+) QFT-GIT (+). Supernatant of peripheral blood mononuclear cell culture was separated with and without stimulation of ESAT-6 and CFP-10, and IL-18 and IL-27 levels were measured with enzyme linked immunoassay (ELISA) test. Results The study group included 22 boys and 18 girls with mean age 4.25 ± 0.9 years. IL-18 and IL-27 levels were statistically significant in ESAT-6/CFP-10-stimulated supernatants of peripheral blood mononuclear cell (PBMC) samples among the three groups (p = 0.000, p = 0.007, respectively). IL-18 levels between the TST (-) QFT-GIT (-) and TST (+) QFT-GIT (+) groups were significantly different (p = 0.026). Both IL-18 and IL-27 levels were significantly different between ESAT-6/CFP-10 stimulated PBMC supernatants of TST (-) QFT-GIT (-) and TST (+) QFT-GIT (-) groups (p = 0.000, p = 0.003, respectively). Conclusion Low IL-18 and IL-27 responses of peripheral blood mononuclear cells in children with Bacillus Calmette-Guérin (BCG) vaccine may play a role in Mycobacterium tuberculosis infection after in-house contact. © 2019 by Georg Thieme Verlag KG, Stuttgart. New York.