Browsing by Author "Karaaslan, MA"

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    Detecting micronuclei frequency in some aquatic organisms for monitoring pollution of Izmir Bay (Western Turkey)
    (SPRINGER) Arslan, ÖC; Parlak, H; Katalay, S; Boyacioglu, M; Karaaslan, MA; Guner, H
    Micronuclei tests is a system of mutagenicity testing used for determining the pollution and chemicals causing changes in DNA fragments such as micronuclei in the cytoplasm of interphase cells. Damage caused on the DNA by genotoxic pollutants is the first consequence occurring in the aquatic organisms. Thus, it was attempted to determine whether pollution affected the erythrocytes and gills of fish Gobius niger and haemolymph and gills of mussels Mytilus galloprovincialis living in Izmir Bay at the level of DNA by the means of micronuclei (MN) test. Organisms used in the MN test were collected from seven locations (Alsancak, Alaybey Shipyard, Karsiyaka, Bostanli, Goztepe, Konak and Pasaport) which are known as the most polluted part of inner Bay of Izmir (Western Coast of Turkey). According to the results of the present study, frequency of MN was found at high level in Alaybey Shipyard and Pasaport where wastes from existing dockyard contributed to high level of pollution. In conclusion, this study indicates that the micronuclei test gives sensitive results in monitoring the pollution, especially the pollution of harbor, and thus it might be used as standard method in regular monitoring of pollution of coastal ecosystem.
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    Assessment of micronuclei induction in peripheral blood and gill cells of some fish species from Aliaga Bay Turkey
    (PERGAMON-ELSEVIER SCIENCE LTD) Arslan, ÖÇ; Boyacioglu, M; Parlak, H; Katalay, S; Karaaslan, MA
    The aim of this paper was to assess the biological damages in fish caused by various mutagenic agents present in polluted waters of Aliaga Bay. For this purpose, micronuclei (MN) test was performed using peripheral erythrocytes and gill cells of different fish specimens caught from both polluted and relatively clean sites from Aliaga Bay (Turkey). Micronuclei tests is a system of mutagenicity testing used for determining changes in DNA fragments such as micronuclei in the cytoplasm of interphase cells caused by the pollution and chemicals in the environment. Thus, it was attempted to determine whether pollution affected the erythrocytes and gills of fish living in Aliaga Bay at the level of DNA by the means of micronuclei (MN) test. According to the results of present study, frequency of MN was found at high level in polluted site. In conclusion, this study indicates that the micronuclei test gives sensitive results in monitoring the pollution, especially the pollution of harbor, and thus it might be used as standard method in regularly monitoring pollution of coastal ecosystem. (C) 2015 Elsevier Ltd. All rights reserved.
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    Phytotoxicity of Water and Sediment from Nif Brook (Izmir, Turkey) on Green Algae Desmodesmus (=Scenedesmus) Subspicatus
    (FOUNDATION ENVIRONMENTAL PROTECTION & RESEARCH-FEPR) Katalay, S; Boyacioglu, M; Arslan, OC; Parlak, H; Karaaslan, MA
    The water of Nif Brook (Izmir, Turkey) is polluted by industrial, domestic, and agricultural sources. As the water of the brook is used for domestic and industrial water supply as well as for irrigation, it is of great importance to know the toxicity potential for the natural populations. For this purpose the standard test. protocol for the short term phytotoxicity test method OECD 201 Algal Growth Inhibition has been evaluated with cultures of green algae Desmodesmus (=Scenedesmus) subspicatus as the representative of the first trophic level. The test has been used to assess the toxicity of both water and sediment samples from the brook. Five sampling sites from the brook were selected considering the type of pollution sources. The water samples were tested with 10, 20, 40, 60, 80 and 100 % dilutions in a test medium. Extracts of sediment samples were assayed in three different concentrations (20, 40 and 100 mg/mL). The effects on the growth of D. subspicatus were evaluated by scoring cell numbers under the light microscope with a Neubauer haemocytometer counting chamber. According to the results, the water samples stimulated the algal growth (except station 1); although all sediment samples inhibited the growth of populations, in several grades.