Browsing by Author "Karaboz, I"

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    ISOLATION AND SCREENING FOR ANTI-MICROBIAL ACTIVITIES OF CULTURABLE MESOPHILIC Streptomyces STRAINS FROM NORTH CYPRUS SOILS
    Oskay, M; Tamer, AU; Karaboz, I
    A total of 249 different Streptomyces strains were isolated from different sites of the North Cyprus habitats for their antimicrobial potential. Out of these, 66 isolates exhibited inhibitory activity against at least one of the tested microorganisms. Approximately, 51% isolates produced antibacterial substances against Gram-positive bacteria, 6% against Gram-negative bacteria, and 23% against both Gram-negative and Gram-positive bacteria, whereas 18% of isolates showed antifungal activity. According to the spectrum of broadness, the two most active isolates were selected, and designed as KGG13 and KVK11. A great variety of morphological, physiological and biochemical features of selected strains were determined for their taxonomic position, and obtained data strongly suggested that these strains belong, to the genus Streptomyces, confirmed by their antimicrobial activity in batch culture. In order to standardize the antibiotic production, some cultural conditions, such as the effect of different temperatures, nature of carbon sources, pH value, and time incubation in h, were determined. The highest antimicrobial activities were obtained when glucose and glycerol at 1% (w/v) was used as sole carbon source, at pHs 7.3 and 7.5 for the strains KGG13 and KVK11, respectively.
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    Production of laccase from Trametes trogii TEM H2: a newly isolated white-rot fungus by air sampling
    Kocyigit, A; Pazarbasi, MB; Yasa, I; Ozdemir, G; Karaboz, I
    This work represents the first report of isolation of potential laccase producers by air sampling using media supplemented with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) and guaiacol for laccase production and secretion indicators. Nine fungal isolates showed positive reactions with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) and guaiacol. The isolate named TEM H2 exhibited the largest and intensive oxidation zones with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) (85 mm) and guaiacol (66 mm) and therefore it was selected for detailed investigations. The strain was identified as Trametes trogii TEM H2 due to the morphological characteristics and the comparison of internal transcribed spacer ribosomal DNA gene sequences. The laccase production was screened in different liquid cultures. The best laccase production medium was determined as soluble starch yeast extract medium in which laccase production was reached to a maximum level (989.6 U l1) on the 8th day of cultivation. Effects of different initial pH values on laccase production were tested. Optimum pH value for laccase production in soluble starch yeast extract medium was determined as pH 3.0 with 15425.0 U l1laccase production at 12th day of cultivation. In addition, effects of eight inducers (veratryl alcohol, ferulic acid, 1-Hydroxybenzotriazole, syringic acid, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate), 1 mmol l1 CuSO4, 3% ethanol, guaiacol) were examined. Only cultures with 2,5-xylidine exhibited 1.9 fold increase in laccase activity reaching to 28890.0 U l1. ((c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
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    DECOLORIZATION OF VARIOUS LEATHER DYES AND LEATHER INDUSTRY EFFLUENT BY Trametes trogii TEM H2
    Pazarbasi, MB; Kocyigit, A; Ozdemir, G; Yasa, I; Karaboz, I
    Decolorization of Acid Blue 7 which is used widely in leather industry was investigated as a model for a decolorization system using soluble starch yeast extract medium under agitated and static conditions with Trametes trogii TEM H2. The effects of different physico-chemical parameters were tested and optimal decolorization rates occurred at pH 5.0 and at 27 degrees C. Decolorization of Acid Blue 7 under agitated and static conditions was determined to be 99.9% and 63.5%, respectively. Decolorization was associated with laccase activity which reached 1110.3 U/L in agitated cultures in the presence of Acid Blue 7 on the 6th day of cultivation. T. trogii TEM H2 was further evaluated for the decolorization of 8 other leather dyes, such as Acid Black 210, Acid Green 20, Acid Yellow 36, Acid Black 24, Acid Black 234, Acid Violet 17, Acid Blue 134, Acid Brown 349, and a mixture of Acid Blue 7 with these 8 leather dyes and leather industry effluents. The decolorization rates after 24 h for the dye mixture and the effluent (10%) were 88% and 48%, respectively. The strain was considered as a good candidate for biodegradation and bioremediation of leather dye-polluted effluents due to its laccase production and decolorizing ability.
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    Phenotypic and molecular characterization of luminous bacteria isolated from Izmir Gulf in Turkey
    Omeroglu, EE; Karaboz, I; Sukatar, A; Uzel, A; Sayan, M; Sanlidag, T
    Luminous bacteria were isolated from invertebrates and coastal seawater samples in the summer season 2004, from Izmir Gulf in Turkey. The seawater samples were concentrated by using 0.22-mu pore-sized nitrocellulose membrane filters. The concentrated seawater samples and intestinal contents of Holothuria tubulosa were inoculated on Seawater Complete (SWC) and BOSS media. The agar plates were incubated at 25 degrees C for 48 h. After incubation, bioluminescent bacteria were isolated in a dark room, then purified and stored on a suitable medium. Bioluminescent isolates were identified with a polyphasic approach by using morphological, cultural, biochemical and molecular characteristics, in addition to 16SrDNA sequencing. Finally, the bioluminescent marine bacteria isolated from intestinal contents of H. tubulosa and seawater samples were identified as Vibrio harveyi TEM S1 and TEM O5 strains, respectively.