Browsing by Author "Karaca, F"

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    The role of hypoxia related angiogenesis in uterine smooth muscle tumors
    Uluer, ET; Inan, S; Ozbilgin, K; Karaca, F; Dicle, N; Sanci, M
    Mechanisms of hypoxia-related angiogenesis are important for uterine smooth muscle tumors. Factors that are related to angiogenesis during hypoxia include vascular endothelial growth factor (VEGF), hypoxia inducible factor 1 alpha (HIF1 alpha), T-cell intracellular antigen1 (TIA1), eukaryotic translation initiation factor 2 alpha (eIF2 alpha) and thrombospondin 1 (TSP1). We investigated immunoreactivities of VEGF, HIF1 alpha, TIA1, eIF2 alpha and TSP1 using an indirect immunoperoxidase method for formalin fixed, paraffin embedded tumors that had been diagnosed as leiomyoma (LMY), cellular leiomyoma (CLM) or leiomyosarcoma (LMS). TSP1 immunoreactivity was scored as moderate, mild or minimal, while VEGF, eIF2 alpha and TIA1 immunoreactivities were scored as mild, moderate and strong in LMY, CLM and LMS samples, respectively. HIF1 alpha immunoreactivity was scored as mild to minimal in LMY, CLM and LMS samples, but showed no statistically significant differences among samples. Although angiogenic factors showed strong immunohistochemical staining intensity in LMS, anti-angiogenic factors showed minimal immunohistochemical intensity. There was no difference in HIF-1 alpha immunoreactivity compared to LMY, CLM and LMS samples. We suggest that HIF1 alpha protein synthesis could be suppressed by eIF2 alpha and TIA1. Furthermore, VEGF could be activated by pathways such as COX2, Ras, NF-kappa B or c-myc instead of HIF1 alpha. Angiogenesis could trigger and accelerate tumor development; therefore, anti-angiogenic therapy could be useful for treatment of tumors.
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    The higher heparin-binding epidermal growth factor (HB-EGF) in missed abortion
    Ozbilgin, K; Karaca, F; Turan, A; Köse, C; Vatansever, S; Ozcakir, T
    Objective: Heparin-binding epidermal growth factor (HB-EGF) has pleiotropic biological functions in the female reproductive tract. HB-EGF has a function in the menstruation cycle, implantation, decidualization, placenta development, and also inhibition of apoptosis. This study aims to investigate a possible role of HB-EGF in missed abortion. Materials and methods: Decidual and placental tissue samples were obtained from women with unwanted pregnancy as the control group and from women with missed abortions as the patient group. Immunohistochemistry was utilized to compare HB-EGF expression of fibroblast and decidual cells in uterine decidual stroma and fibroblasts and mesenchymal cells in placental villous stroma; the TUNEL technique was used to detect apoptotic cells within the decidual and placental tissues of the two groups. Results: It was demonstrated that HB-EGF expression in both uterine decidual stroma and placenta stroma was increased in the missed abortion group (142.70 +/- 12.80; 116.10 +/- 14.16, respectively), compared with the normal pregnancy group (101.60 +/- 14.18; 81.60 +/- 10.74, respectively). It was also shown that there was no difference in TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) positive cells between the uterine decidual stroma (11.4 +/- 3%; 13.6 +/- 3%, respectively), placental villous stroma (13.7 +/- 3%; 15.9 +/- 3%, respectively), and cytotrophoblast-syncytiotrophoblast cells (7.3 +/- 2; 9.8 +/- 3, respectively) of the two groups. Conclusion: This data supports the hypothesis that increased HB-EGF expression in a missed abortion may prevent the discharge of the dead fetus. Copyright (C) 2014, Taiwan Association of Obstetrics & Gynecology. Published by Elsevier Taiwan LLC. All rights reserved.
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    Increased expression of VEGF, iNOS, IL-1β, and IL-17 in a rabbit model of gastric content-induced middle ear inflammation
    Basoglu, MS; Eren, E; Aslan, H; Kolatan, HE; Özbay, C; Inan, S; Karaca, F; Öztürkcan, S; Katilmis, H
    Objective: To investigate the histopathological changes and the expression of vascular endothelial growth factor (VEGF), inducible NO-synthase (iNOS), endothelial NO-synthase (eNOS), interleukin (IL)-1 beta, and IL-17 in the rabbit middle ear mucosa after direct gastric content exposure. Methods: Exploratory controlled study in which histological and immunochemical features were studied after gastric content-induced inflammation was established in rabbits. Sixteen healthy rabbits were divided into two equal groups. Gastric contents of an animal were injected into the middle ear of the same animal for 20 days. Saline was injected into the middle ear of the animals in the control group. The rabbits were humanely killed on day 27. Inflammation was assayed by light microscopy. Immunochemical staining was performed for VEGF, iNOS, eNOS, IL-1 beta, and IL-17 expression. Experimental and control animals were examined using the same protocol. Results: The expression levels of VEGF, INOS, IL-1 beta, and IL-17 differed significantly between the experimental and control groups (p = 0.018, p = 0.010, p = 0.002, and p = 0.002, respectively). The expression level of eNOS was not significantly different between the two groups (p = 0.132). Conclusion: This study demonstrates that gastroesophagial reflux induced middle ear inflammation is associated with increased expression of VEGF, IL-1 beta, IL-17, and iNOS. (C) 2011 Elsevier Ireland Ltd. All rights reserved.