Browsing by Author "Karadadas, N"

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    Evaluation Of VEGF, EGF-R AND TGF-α In Placenta Of Pregnancies With Preeclampsia By Immunohistochemistry
    Çirpanli, T; Akercan, F; Terek, MC; Özcakir, HT; Giray, G; Sagol, S; Karadadas, N
    Objective: The aim of the study is to determine VEGF, EGFR and TGF-alpha with the immunohistochemical staining in placenta biopsies of patients with preeclampsia. Design: Prospective case control study. Materials and Methods: Biopsies from the placenta were obtained from ten patients with preeclapmsia and ten patients of control group during the cesarean section. Placenta biopsies were examined for VEGF, EGF-R and TGF-alpha distribution with avidin-biotinperoxidase immunohistochemistry. The staining intensities were graded semi-quantitatively by the two histologists who were blinded to the groups and the H-score was calculated by light microscopic examination. Results: The VEGF, EGFR and TGF-alpha expression was significantly higher in placenta biopsies of preeclamptic patient compared to controls (271.2 +/- 22.65 versus 201.9 +/- 12.33, p=0.000; 186.3 +/- 4.98 versus 150.3 +/- 5.7, p=0.000; 185.1 +/- 7.48 versus 169.2 +/- 6.19, p=0.000; respectively). Conclusion: Immunostaining of VEGF, EGF-R and TGF-alpha is significantly higher in placenta biopsies in patients with preeclampsia.
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    The immunohistochemical evaluation of VEGF in placenta biopsies of pregnancies complicated by preeclampsia
    Akercan, F; Cirpan, T; Terek, MC; Ozcakir, HT; Giray, G; Sagol, S; Karadadas, N
    Objective The study was designed to determine the protein levels of vascular endothelial growth factor (VEGF) in the placenta biopsies of patients with preeclampsia and compare with normal controls. Design Prospective cohort study. Methods The placental biopsies were obtained from ten patients with preeclampsia and ten patients of control group at the time of delivery. Avidin-biotin-peroxidase immunohistochemistry was then performed to identify levels of VEGF protein within the tissue and a semi-quantitative method was devised to score the amount of staining present in the sample. Two histopathologists who were blinded to the groups were asked to score each sample for the intensity of staining and the number of cells stained in a randomly selected per high-power fields of each sample. The resulting H-score was computed as a product of intensity and percent of cells stained. Results The VEGF expression was significantly higher in placenta biopsies of preeclamptic patients compared to that of controls (271.2 +/- 22.65 vs. 201.9 +/- 12.33, P = 0.000). Conclusion Immunostaining of VEGF is significantly higher in placenta biopsies of patients with preeclampsia.