Browsing by Author "Kasap E."
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Item Angiotensin-converting enzyme genotype and acute pancreatitis in Turkey(2009) Kasap E.; Akyildiz M.; Tekin F.; Akarca U.The renin-angiotensin system (RAS) has been implicated in the pathogenesis of acute and chronic pancreatitis. Angiotensin-converting enzyme (ACE) is the key enzyme which activates RAS. The ACE intron 16 insertion/ deletion (I/D) polymorphism is associated with ACE activity and is considered to be a risk factor for several inflammatory processes. We investigated this polymorphism in 68 patients with acute pancreatitis (AP) and 157 healthy Turkish control subjects. Patients were evaluated with ultrasonography, abdominal tomography and laboratory markers and grouped by status for diabetes mellitus (DM), hypertension (HT), and both these diseases and by etiology. Genotyping of the I/D polymorphism was performed by polymerase chain reaction (PCR). The DD genotype was more prevalent in healthy controls, however, genotype II was significantly more frequent in AP patients (p <0.05). In severe AP patients, the genotype II frequency was significantly higher than in controls (p <0.05). Acute pancreatitis patients with both DM and HT had lower frequencies of genotype DD and of the D allele, and higher frequencies of genotype II and of the I allele than patients with either DM or HT (p <0.05).Item Cholangitis of pancreatitis? Does the angiotensin-converting enzyme genotype favor either?(2009) Kasap E.; Akyildiz M.; Akarca U.Acute cholangitis and pancreatitis are serious complications of gallstones, with considerable morbidity and mortality. Angiotensin-converting enzyme (ACE) is an exopeptidase that is important in regulating blood pressure, metabolizing bradykinin and in maintaining an inflammatory response. To determine whether the ACE genotype determines occurrence of cholangitis or pancreatitis we examined ACE I/D genotypes in 31 patients who had cholangitis, 44 patients with biliary pancreatitis and 157 healthy individuals. The patients had been hospitalized at the Department and Intensive Care Faculty of Medicine, Ege University, Izmir, Turkey. The patients were recalled 4 years later and their prognosis was evaluated. The ACE II genotype was found at a higher frequency in the cholangitis and biliary pancreatitis patients when compared with the healthy subjects (p <0.05). There was no significant difference between cholangitis and biliary pancreatitis cases regarding the genotype and allele distribution (p >0.05). Recurrence of infection occurred more frequently in the patients with the DD genotype, although it was not significant according to the first assessment (p >0.05). The ACE gene polymorphism did not seem to favor development of either cholangitis or pancreatitis.Item The frequency of sexual-physical abuse history in irritabl bowel syndrome patients and diagnostic relation with major depressive disorder history; [İrritabl barsak sendromu hastalarinda cinsel-fiziksel istismar sikliǧi ve majör depresif bozukluk öyküsü tani ilişkisi](2010) Eker M.Ç.; Kasap E.; Elbi H.; Bor S.Objective: IBS is a functional disorder which is one of the most common causes of gastroentrology doctor visits. Besides multiple factors suggested for the etiology of IBS, the co-morbidity of psychiatric illnesses, of which MDD is most the frequent, is also noted. Being exposed to sexual or physical abuse is also considered as a risk factor for the development of IBS. Methods: In this study the differences between IBS patients and non-IBS control cases are evaluated regarding the history of major depressive disorder and sexual or physical abuse. Fiftyfour IBS and thirty control cases (mean age for IBS patients 41±11; for control group 34±12 years; p<0.05) were recruited for the study. Results: IBS patients had a history of MDD more frequently than the control cases (69% for the IBS group, n=37; 30% for the control group, n=9; p<0.001]. However, two groups did not differ regarding the history of sexual abuse (20% for the IBS group, n=11; (13% for the control group, n=4; p>0.05) or physical abuse (19% for the IBS group, n=10; 13% for the control group, n=4; p>0.05). Discussion: IBS and MDD are frequently co-morbid and this co-morbidity suggests a possible common point for the etiopathogenesis for both disorders. Nevertheless, the contribution of sexual or physical abuse to the co-morbidity of these disorders seems to be inconclusive. It can be suggested that sexual and physical abuse history do not have fundemental importance for the development of IBS in our patients.Item GSTP1 gene methylation profiles in Helicobacter pylori (+) and (-) antral intestinal metaplasia and distal gastric tumour patients in Turkish population(2012) Asik-Sen G.; Kasap E.; Orenay-Boyacioglu S.; Korkmaz M.; Kahraman E.; Unsal B.; Yüksel-Saritas E.; Yuceyar H.Background/Aims: Gastric cancer (GC) is the second most common malignancy worldwide, with a high mortality rate. The incidence of GC has declined in the western countries during the last decades. The glutathione S-transferases comprise a group of enzymes that are critical in the detoxification of carcinogens. In this study we aimed at the relationship GSTP-1 methylation in patients with intestinal metaplasia with and without Helicobacter pylori infection, gastric cancer and controls. Methodology: The methylation status of GSTP1 gene was analyzed by methylation specific PCR after bisulfate modification in H. pylori (+) (n=25) and (-) (n=25) intestinal metaplasia (IM) patients, GC (n=25) and control subjects (n=15) between September 2009 to November 2011. Results: During the study period 90 patients who underwent endoscopic examination were included in the study. When we considered the GSTP1 gene methylation profile in all of the groups; 26 (28%) patients had methylated GSTP1 gene, 31 (34%) patients had unmethylated GSTP1 gene and 33 (36%) patients had heterogeneously methylated GSTP1 gene. Conclusions: GSTP1 gene methylation profile is not appropriate for early diagnosis of cases with gastric cancer. © H.G.E. Update Medical Publishing S.A.Item Aurora kinase A (AURKA) and never in mitosis gene A-related kinase 6 (NEK6) genes are upregulated in erosive esophagitis and esophageal adenocarcinoma(2012) Kasap E.; Boyacioglu S.Ö.; Korkmaz M.; Yuksel E.S.; Ünsal B.; Kahraman E.; Özütemiz Ö.; Yuceyar H.Gastroesophageal reflux disease is a risk factor for esophageal adenocarcinoma yet studies that have investigated the relationship between erosive esophagitis and esophageal adenocarcinoma have usually focused on symptom-related evidence or polymorphisms. There are no epigenetic gene expression studies on this topic. In this study, we aimed to evaluate the relationship between erosive esophagitis and esophageal adenocarcinoma to identify whether there is a genetic predisposition for esophageal adenocarcinoma. The Human Epigenetic Chromatin Modification Enzyme RT 2 Profiler™ PCR array (PAHS-085A) was used to detect the expression of 84 key genes encoding enzymes. This was carried out prospectively for samples from 60 patients (20 patients as a control group, 20 patients with erosive esophagitis and 20 patients with esophageal adenocarcinoma). AURKA, AURKB, NEK6 were expressed at significantly higher levels in esophageal adenocarcinoma compared to the control group. MBD2 was expressed at significantly lower levels in the esophageal adenocarcinoma group compared to the control group. AURKA, AURKC, HDAC9 and NEK6 were expressed at significantly higher levels in erosive esophagitis compared to the control group. There was no difference in upregulated gene expression between the erosive esophagitis and esophageal adenocarcinoma. MBD2 was significantly downregulated in esophageal adenocarcinoma compared to erosive esophagitis. NEK6 and AURKA were significantly upregulated in esophageal adenocarcinoma and erosive esophagitis compared to the control group. This is a novel study on the genetic predisposition for erosive esophagitis and esophageal adenocarcinoma. AURKA and NEK6 are two promising genetic markers for erosive esophagitis and esophageal adenocarcinoma.Item Does Helicobacter pylori treatment improve the symptoms of globus hystericus?(2012) Kasap E.; Ayhan S.; Yüceyar H.Background/aims: Globus hystericus is a feeling of tension in the throat, irrelevant of swallowing, persisting for at least 12 weeks. Since the cause of globus hystericus is not fully described, the treatment is controversial. We aimed in this study to determine the symptoms of gastroesophageal reflux disease, upper gastrointestinal endoscopic findings, prevalence of Helicobacter pylori, and post-treatment symptoms (symptoms of gastroesophageal reflux and/or Helicobacter pylori) in patients with a diagnosis of globus hystericus. Materials and Methods: One hundred twenty three patients were recruited from the archives of the Department of Gastroenterology and Endoscopy at Celal Bayar University Medical School between January 2009 and August 2010. Results: Helicobacter pylori was positive in 75 (60%) of 123 patients with globus hystericus. Helicobacter pylori (+) patients had significantly more heartburn, regurgitation, and inlet patch in upper esophagus than Helicobacter pylori (-) patients. Significantly more Helicobacter pylori (-) patients had normal endoscopy findings when compared to Helicobacter pylori (+) patients. While 27 (50%) of Helicobacter pylori-eradicated patients had regressing globus symptoms, 12 (17.3%) of them did not have any regression in globus symptoms. Improvement in symptoms showed a positive correlation with Helicobacter pylori eradication (p=0.001). Conclusions: Helicobacter pylori rate among cases with globus sensation was similar to values in the general population. Helicobacter pylori eradication was found to decrease globus symptoms.Item Does Helicobacter pylori eradication improve the symptoms of globus hystericus?(2013) Kasap E.; Ayhan S.; Yüceyar H.Background/aims: Globus hystericus is a feeling of a tension in the throat, irrelevant of swallowing, persisting for at least 12 weeks. Since the cause of globus hystericus is not fully described, a controversy exists about the treatment options. We aimed in this study to determine the symptoms of gastroesophageal reflux disease, the upper gastrointestinal endoscopic findings, prevalence of Helicobacter pylori, and the post-treatment symptoms (symptoms of gastroesophageal reflux disease and/or Helicobacter pylori) in patients with a diagnosis of globus hystericus. Materials and Methods: In this study, we retrostectively evaluated the medical records of 123 patients who, after the initial diagnosis of globus sensation (globus hystericus) underwent endoscopic examination in the Department of Gastroenterology and Endoscopy at Celal Bayar University Medical School, and thereafter, were given Helicobacter pylori eradication therapy and were followed in the Gastroenterology outpatient clinic, between January 2009 and August 2010. Results: Helicobacter pylori was positive in seventy-five (60%) of the 123 patients. Heartburn, regurgitation, and inlet patch in the upper esophagus were significantly more frequent in Helicobacter pylori (+) patients than in Helicobacter pylori (-) patients with globus hystericus. Normal endoscopy findings were significantly more common in Helicobacter pylori (-) patients than in Helicobacter pylori (+) patients with globus hystericus. Of patients with eradicated Helicobacter pylori, 27 (50%) reported that globus symptoms had disappeared, while 12 (17.3%) of them had not observed any regression of the symptoms. Improvement of symptoms had a positive correlation with Helicobacter pylori eradication (p=0,001). Conclusions: Helicobacter pylori prevalence among cases with globus sensation is similar to that in the general population. However, Helicobacter pylori eradication was found to decrease the symptoms of globus.Item The efficacy of complete blood count parameters in the diagnosis of tubal ectopic pregnancy(Studio K Krzysztof Molenda, 2014) Eskicioğlu F.; Özdemir A.T.; Turan G.A.; Gür E.B.; Kasap E.; Genç M.Objective: Ectopic pregnancy (EP) is the major cause of maternal morbidity and is responsible for maternal mortality in the first trimester. In order to reduce undesirable results, it is necessary to find rapid and accurate, non-surgical diagnostic tests for EP. The goal of the study was to investigate the differences in complete blood count parameters between tubal EPs and healthy pregnancies in be used in the diagnosis of ectopic pregnancy. Study design: White blood cell (WBC), neutrophil, monocyte, lymphocyte, platelet (PLT) counts, mean PLT volume (MPV) and PLT distribution width (PDW) levels in the complete blood count samples have been obtained from subjects with diagnosed tubal EP (n=78; study group) and women with healthy intrauterine gestations (n=79; control group). Statistical comparisons between groups were performed using the t test. Results: PDW levels were found to be significantly higher in the control group than EP (p<0.001). However, no differences between the study and control groups with regard to PLT and MPV levels were observed. WBC levels were found to be significantly higher in the EP group as compared to controls (p<0.001). When leukocyte differentials were compared, monocyte counts in the EP group were significantly higher than in controls (p=0.005). No statistically significant differences in neutrophil and lymphocyte values were observed in either group. Conclusion: PDW as an indicator of PLT activation is lower in tubal EP than intrauterine pregnancy so, possibly, endometrial invasion in the intrauterine pregnancy needs more PLT activation. Monocyte counts are higher in tubal EP, indicating that monocyte activation in the pathophysiology of EP could be effective in the formation of tubal motility and microenvironment regulation. © Polskie Towarzystwo Ginekologiczne.Item Schizophrenia and gastroesophageal reflux symptoms(Medknow Publications, 2015) Kasap E.; Ayer A.; Bozoʇlan H.; Ozen C.; Eslek I.; Yüceyar H.Background: Psychological factors and psychiatric disorders play a role in a variety of gastrointestinal illnesses, including esophageal diseases. Aims: The aim of the present study was to evaluate the frequency of gastroesophageal reflux disease symptoms in patients with schizophrenia in Turkey. Patients and Methods: Ninety-eight patients with schizophrenia and one hundred control individuals were enrolled in the study, which was undertaken at the Manisa State Hospital for Mental Health and Neurological Disorders and Celal Bayar University Gastroenterology Department. Case and control subjects alike underwent 30-45 min oral interviews conducted by a designated study coordinator (E.K.). The coordinator gathered information about demographic characteristics, social habits, and a large variety of symptoms suggestive of reflux disease or other gastrointestinal conditions. Results: In terms of reflux symptoms, cough was the only significant association in schizophrenic patients than controls. Heartburn and regurgitation were more frequent in schizophrenic patients who smoked than in controls who were smokers. However, the prevalence of reflux symptoms in cigarette smokers versus nonsmoker patients with schizophrenia was similar. Heartburn and/or regurgitation occurred more frequently in patients with schizophrenic than controls with alcohol use. Conclusions: Psychiatric disorders might indirectly affect esophageal physiology through increased consumption of alcohol and nicotine.Item Never in mitosis gene A-related kinase 6 and aurora kinase A: New gene biomarkers in the conversion from ulcerative colitis to colorectal cancer(Spandidos Publications, 2015) Gerçeker E.; Boyacioglu S.O.; Kasap E.; Baykan A.; Yuceyar H.; Yildirim H.; Ayhan S.; Ellidokuz E.; Korkmaz M.Ulcerative colitis (UC) is an important risk factor for colorectal cancer (CRC). Histone modifications are one of the epigenetic mechanisms that may have key roles in the carcinogenesis of CRC. At present, there are no studies comparing histone modification patterns of UC and CRC in the literature. Therefore the aim of the present study was to investigate whether genes, particularly those involved in histone modification, have value in patient monitoring with regards to CRC development in UC. Key gene expressions of the histone modification enzyme were assessed and compared in CRC, UC and control groups using the RT-PCR array technique. Patients were divided into subgroups based on the extent and duration of the disease and inflammatory burden, which are considered risk factors for CRC development in UC patients. In UC and CRC groups, a significantly higher overexpression of the NEK6 and AURKA genes compared to the control group was identified. In addition, there was a significantly higher overexpression of HDAC1 and PAK1 genes in the UC group, and of HDAC1, HDAC7, PAK1 and AURKB genes in the CRC group. NEK6, AURKA, HDAC1 and PAK1 were significantly overexpressed in patients with a longer UC duration. Overexpression of AURKA and NEK6 genes was significantly more pronounced in UC patients with more extensive colon involvement. HDAC1, HDAC7, PAK1, NEK6, AURKA and AURKB are important diagnostic and prognostic markers involved in the carcinogenesis of CRC. HDAC1, PAK1, NEK6 and AURKA may be considered as diagnostic markers to be used in CRC screening for UC patients.Item Alteration in methylation pattern of retinoblastoma 1 gene promotor region in intestinal metaplasia with or without helicobacter pylori and gastric cancer patients(Wroclaw University of Medicine, 2016) Boyacioglu S.O.; Kasap E.; Yuceyar H.; Korkmaz M.Background. Helicobacter pylori, intestinal metaplasia (IM), and gene methylation play important roles in gastric carcinogenesis. However, the association among H. pylori infection, IM, gastric cancer (GC), and gene methylation is not fully understood. Cell cycle control involving retinoblastoma 1 (RB1) gene is one of the main regulatory pathways reported to be altered in gastric carcinogenesis. Objectives. The purpose of this research is to assess the methylation status of RB1 gene in GC and IM with or without H. pylori infection, and to discuss the possible role of H. pylori-induced RB1 gene methylation in the mechanism of gastric carcinogenesis. Material and Methods. The methylation profile of RB1 gene was analyzed by sodium bisulfite modification and methylation-specific PCR in GC (n = 24), IM patients with H. pylori positive (n = 20) and negative (n = 20), and control subjects (n = 20). Results. According to methylation levels in RB1 gene; the high correlation values were detected between H. pylori positive-IM group and GC group, and between H. pylori positive-IM and H. pylori negative-IM groups (p < 0.05). No correlations between H. pylori negative-IM and GC groups and between GC and control groups were detected in methylation status of RB1 gene. Conclusions. High methylation levels in RB1 gene in H. pylori positive individuals may suggest an elevated risk of gastric cancer occurrence. © Copyright by Wroclaw Medical University.Item The potential role of the NEK6, AURKA, AURKB, and PAK1 genes in adenomatous colorectal polyps and colorectal adenocarcinoma(Springer Science and Business Media B.V., 2016) Kasap E.; Gerceker E.; Boyacıoglu S.Ö.; Yuceyar H.; Yıldırm H.; Ayhan S.; Korkmaz M.Colorectal adenomatous polyp (CRAP) is a major risk factor for the development of sporadic colorectal cancer (CRC). Histone modifications are one of the epigenetic mechanisms that may have key roles in the carcinogenesis of CRC. The objective of the present study is to investigate the alternations in the defined histone modification gene expression profiles in patients with CRAP and CRC. Histone modification enzyme key gene expressions of the CRC, CRAP, and control groups were evaluated and compared using the reverse transcription PCR (RT-PCR) array method. Gene expression analysis was performed in the CRAP group after dividing the patients into subgroups according to the polyp diameter, pathological results, and morphological parameters which are risk factors for developing CRC in patients with CRAP. PAK1, NEK6, AURKA, AURKB, HDAC1, and HDAC7 were significantly more overexpressed in CRC subjects compared to the controls (p < 0.05). PAK1, NEK6, AURKA, AURKB, and HDAC1 were significantly more overexpressed in the CRAP group compared to the controls (p < 0.005). There were no significant differences between the CRAP and CRC groups with regards to PAK1, NEK6, AURKA, or AURKB gene overexpression. PAK1, NEK6, AURKA, and AURKB were significantly in correlation with the polyp diameter as they were more overexpressed in polyps with larger diameters. In conclusion, overexpressions of NEK6, AURKA, AURKB, and PAK1 genes can be used as predictive markers to decide the colonoscopic surveillance intervals after the polypectomy procedure especially in polyps with larger diameters. © 2015, International Society of Oncology and BioMarkers (ISOBM).Item Prevalence of gastroesophageal reflux disease in a country with a high occurrence of Helicobacter pylori(Baishideng Publishing Group Co, 2017) Bor S.; Kitapcioglu G.; Kasap E.AIM: To evaluate the prevalence of gastroesophageal reflux disease (GERD) with additional symptoms, relationship with Helicobacter pylori (H. pylori) of this country-wide study. METHODS: Data from 3214 adults were obtained with validated questionnaire. Eight hundred and forty-one subjects were randomized to be tested for H. pylori via the urea breath test. "Frequent symptoms" were defined heartburn and/or regurgitation occurring at least weekly. RESULTS: The prevalence of GERD was 22.8%, frequent and occasional heartburn were 9.3%-12.7%, regurgitation were 16.6%-18.7%, respectively. Body mass index (BMI) ≤ 18.5 showed a prevalence of 15%, BMI > 30 was 28.5%. The GERD prevalence was higher in women (26.2%) than men (18.9%) (P < 0001). Overall prevalence of H. pylori was 75.7%. The prevalence was 77.1% in subjects without symptoms vs 71.4% in subjects with GERD (χ2 = 2.6, P = 0.27). Underprivileged with the lowest income people exhibit a higher risk. CONCLUSION: GERD is common in Turkey which reflects both Western and Eastern lifestyles with high rate of H. pylori. The presence of H. pylori had no effect on either the prevalence or the symptom profile of GERD. Subjects showing classical symptoms occasionally exhibit more additional symptoms compared with those without classical symptoms. © 2017 Baishideng Publishing Group Inc. All rights reserved.Item Determination of drug resistance mutations of NS3 inhibitors in chronic hepatitis c patients infected with genotype; [Genotip 1 ile enfekte kronik hepatit C hastalarinda NS3 inhibitörü ilaçlarin direnç mutasyonlarinin belirlenmesi](Ankara Microbiology Society, 2017) Şanlidaǧ T.; Sayan M.; Akçali S.; Kasap E.; Buran T.; Arikan A.Direct-Acting antiviral agents (DAA) such as NS3 protease inhibitors is the first class of drugs used for chronic hepatitis C (CHC) treatment. NS3 inhibitors (PI) with low genetic barrier have been approved to be used in the CHC genotype 1 infections, and in the treatment of compensated liver disease including cirrhosis together with pegile interferon and ribavirin. Consequently, the development of drug resistance during DAA treatment of CHC is a major problem. NS3 resistant variants can be detected before treatment as they can occumaturally. The aim of this study was to investigate new and old generation NS3 inhibitors resistance mutations before DAA treatment in hepatitis C virus (HCV) that were isolated from CHC. The present study was conducted in 2015 and included 97 naive DAA patients infected with HCV genotype 1, who were diagnosed in Manisa and Kocaeli cities of Turkey. Magnetic particle based HCV RNA extraction and than RNA detection and quantification were performed using commercial real-Time PCR assay QIASypmhony + Rotorgene Q/ArtusHCV QS-RGQ and COBAS Ampliprep/COBAS TaqMan HCV Tests. HCV NS3 viral protease genome region was amplified with PCR and mutation analysis was performed by Sanger dideoxy sequencing technique of NS3 protease codons (codon 32-185). HCV NS3 protease inhibitors; asunaprevir, boceprevir, faldaprevir, grazoprevir, pariteprevir, simeprevir and telap- revir were analysed for resistant mutations by Geno2pheno-HCV resistance tool. HCV was genotyped in all patients and 88 patients (n= 88/97, 91%) had genotype 1. Eight (n= 8/97, 8.2%) and 80 (n= 80/97, 82.4%) HCC patients were subgenotyped as 1 a and 1 b, respectively. Many aminoacid substitutions and resistance mutations were determined in 39/88 (44%) patients in the study group. Q80L, SI 22C/N, SI 38W were defined as potential substitutions (6/88 patients; 7%); R109K, R117C, S122G, 1132V, 1170V, N174S were described as potential resistance (34/88 patients; 39%); V36L, T54S, V55A, Q80H were characterized as resistance (7/88 patients; 8%) and Q80K, A156S were defined as high resistance (3/88 patients; 3%) mutations. Based on resistance and high resistance mutations, clinically significant mutations were defined in 10/88 (11%) of the patients. Our study shows that it is essential to analyse HCV NS3 protease inhibitors drug resistance before DAA treatment of CHC patients. On the other hand, our results pointed out that analysis of NS5A and NS5B genome region mutations may also be required in the near future.Item Treatment of inflammatory bowel disease by leukocytapheresis(Elsevier Ltd, 2017) Gerçeker E.; Yüceyar H.; Kasap E.; Demirci U.; Ekti B.C.; Aydoğdu İ.; Miskioğlu M.Studies about leukocytapheresis have emerged with the need of search for alternatives to conventional treatment in inflammatory bowel diseases (IBD). Leukocytapheresis is a novel non-pharmacologic approach for active ulcerative colitis (UC) and Crohn's disease (CD), in which leukocytes are mechanically removed from the circulatory system. Patients with active IBD treated with leukocytapheresis using a Cellsorba E column between 2012 and 2015, were enrolled in Turkey. In our experience, the results of leukocytapheresis therapy in 6 patients with CD and 20 patients with active UC were overviewed. Leukocytapheresis (10 sessions for remission induction therapy, 6 sessions for maintenance therapy) was applied to the patients with their concomitant medications. Intensive leukocytapheresis (≥4 leukocytapheresis sessions within the first 2 weeks) was used in 30% patients with active severe UC. The overall clinical remission rate in patients with UC was 80%, and the mucosal healing rate was 65%. Patients were followed for an average of 24 months. It was observed that clinical remission has continued in 65% of patients with UC. Mild relapse was observed in 3 patients with UC during follow up period. In 5 patients with CD significant clinical remission was achieved except only one patient. Surgical needs were disappeared in 3 patients with obstructive type Crohn's disease. Adverse events were seen in only 4.3% of 416 sessions. Any concomitant medications did not increase the incidence of adverse events. Our results indicate that leukocytapheresis is efficacious in improving remission rates with excellent tolerability and safety in patients with IBD. © 2017 Elsevier LtdItem Potential role of chromatin remodeling factor genes in atrophic gastritis/gastric cancer risk(AVES İbrahim KARA, 2018) Bilgiç F.; Gerçeker E.; Boyacioglu S.Ö.; Kasap E.; Demirci U.; Yildirim H.; Baykan A.R.; Yüceyar H.Background/Aims: Atrophic gastritis (AG), intestinal metaplasia (IM), and Helicobacter pylori (HP) are the risk factors for the development of gastric cancer (GC). Chromatin remodeling is one of the epigenetic mechanisms involved in the carcinogenesis of GC. The purpose of this study was to investigate the expression profiles of defined chromatin remodeling genes in gastric mucosal samples and their values as gastric carcinogenesis biomarkers. Materials and Methods: In total, 95 patients were included in the study. Patients were divided into 3 groups as: GC group (n=34), AG group (n=36), and control group (n=25). AG group was further divided into subgroups based on the presence of HP and IM in gastric mucosa. Chromatin remodeling gene expressions were analyzed using real-time PCR (RT-PCR) array in all groups. Data were evaluated using the RT-qPCR primer assay data analysis software. Results: EED, CBX3, and MTA1 were more overexpressed, whereas ARID1A, ING5, and CBX7 were more underexpressed in the AG and GC groups compared with the controls. No significant differences were observed between the AG and GC groups concerning the expression of these 6 genes, although the fold change levels of these genes in the GC group were well above than in the AG group. EED, CBX3, and MTA1 were significantly more overexpressed in HP- and IM-positive AG subgroup compared with the HP- or IM-negative AG subgroup. Conclusion: In conclusion, our results provide an evidence of epigenetic alterations in AG. Expressions of EED, CBX3, MTA1, ARID1A, ING5, and CBX7 may be considered as promising markers to be used in GC screening for patients with AG. © Copyright 2018.Item Expression profiles of histone modification genes in gastric cancer progression(Springer Netherlands, 2018) Orenay-Boyacioglu S.; Kasap E.; Gerceker E.; Yuceyar H.; Demirci U.; Bilgic F.; Korkmaz M.Gastric cancer (GC) development can be attributed to several risk factors including atrophic gastritis (AG), intestinal metaplasia (IM), and the presence of Helicobacter pylori (HP). Also, histone modification is an epigenetic mechanism that plays a pivotal role in GC carcinogenesis. In this preliminary study, we aimed to describe the expression profiles of histone modification in the AG, IM, and GC patient groups. A total of 80 patients with AG (n = 27), IM (n = 25), and GC (n = 28) with an additional 20 control subjects were included in the study. Expression profiles of three histone phosphorylation genes (PAK1, NEK6, and AURKA) and five histone deacetylation genes (HDACs 1, 2, 3, 5, and 7) were examined based on the results of Real Time qPCR method. It was observed that AURKA and HDAC2 genes were significantly overexpressed in all groups compared to the control (P < 0.05). In GC patients, overexpression of HDAC2 gene was detected in the absence of metastasis, and overexpression of AURKA, HDAC2, and NEK6 genes was detected in the presence of metastasis. When cancer involvements were compared, significant overexpression of the HDAC2 gene was noted in overall and corpus involvements (P < 0.05). In addition, overexpression of AURKA, NEK6, HDAC1, and HDAC2 genes and underexpression of HDAC5 gene were detected in the antrum involvement (P < 0.05). In conclusion, decreased expression of HDAC5 in GC is reported for the first time in this study, while supporting the existing literature in AURKA, NEK6, HDAC1, and HDAC2 up regulations during GC development. © 2018, Springer Nature B.V.Item Association of interleukin 12B RS3212227 polymorphism with gastric cancer, intestinal metaplasia, and helicobacter pylori infection(Serbian Genetics Society, 2020) Orenay-Boyacioglu S.; Kasap E.; Yuceyar H.; Korkmaz M.Interleukin 12 (IL-12) has a key function in promoting Th1 immune response in the gastrointestinal mucosa. Although cytokine gene polymorphisms are associated with increased risk of gastric cancer (GC), studies on different geographic regions and ethnic groups are not able to draw a consistent result. The current case-control study aims to find out an association between a functional IL-12B rs3212227 polymorphism and the susceptibility and clinical features of the study groups, which are GC, Helicobacter pylori-infected and H. pylori-uninfected intestinal metaplasia (IM). In this study, IL-12B rs3212227 polymorphism was genotyped in 35 GC cases, 25 H. pylori-infected IM patients, 25 H. pylori-uninfected IM patients, and 25 control subjects. PCR-RFLP analysis was performed to find out and compare the polymorphism profiles of case biopsies. There was statistical significance in genotype distributions and allelic frequencies in GC patients with proximal arrest in stomach (p=0.042). The rs3212227 genotypes and allelic frequencies were not correlated with any of the study groups (p>0.05). Other clinical features examined in the GC patients were also not correlated with the rs3212227 genotypes and allelic frequencies (p>0.05). Current findings suggest that IL-12B rs3212227 polymorphism may play a role in GC development. © Serbian Genetics Society.Item Turkish gastroenterology association, pancreas study group, chronic pancreatitis committee consensus report(AVES, 2021) Soytürk M.; Bengi G.; Oǧuz D.; Kalkan I.H.; Yalniz M.; Tahtaci M.; Demir K.; Kasap E.; Oruç N.; Ünal N.G.; Sezgin O.; Özdoǧan O.; Altintaş E.; Yaraş S.; Parlak E.; Şeref Köksal A.; Saruç M.; Ünal H.; Ünsal B.; Günay S.; Duman D.; Yurçi A.; Kacar S.; Filik L.[No abstract available]Item The importance of apoptosis in cancer development and treatment(Istanbul Tip Fakultesi, 2021) Gökbayrak Atay İ.N.; Gezer A.H.; Kasap E.Cancer is one of the most important causes of death in our era. Multifactorial causes are involved in the formation of cancer. The reduction of apoptosis is one of these reasons. Failure to activate apoptosis pathways can lead to resistance to the current treatment approaches of cancers. A better understanding of the molecular events that regulate apoptosis in cancers and cancer therapy form the basis of a more rational approach to the development of molecular targeted therapies in the fight against cancer. © 2021, Turkish Society for Radiation Oncology.