Browsing by Author "Katalay S."
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Item Embryotoxic effects of water and sediment from nif brook (Western, Turkey) on sea urchin paracentrotus lividus(2009) Arslan O.C.; Parlak H.; Katalay S.; Boyacioglu M.; Karaslan M.A.In this investigation embryo toxicity of water and sediment samples collected from Nif Brook of Izmir, Turkey has been studied. Five sampling sites from the brook were selected. Sea urchin embryos were utilized for evaluating the toxicity of water and sediment. The effects on developing embryos were evaluated by scoring developmental defects. The water and sediment samples were tested with 0.001, 0.01, 0.1 dilutions in natural sea water. All water and sediment samples of the brook were assessed to be toxic according to the sea urchin embryotoxicity results. The embryotoxicity of water samples concentration-dependent and significant growth reduction at the early life stages and an increase in larval malformations as skeleton deformities at the pluteus stage were observed (approximately 59% in 0.001 ml/ml from station 5). The most polluted sediment samples displayed a dramatic embryotoxicity, up to approximately 98% developmental arrest in embryos reared in 0.1 g/ml of sediment from station 5. © by PSP Volume 18-No 5a. 2009.Item Detecting micronuclei frequency in some aquatic organisms for monitoring pollution of Izmir Bay (Western Turkey)(2010) Çakal Arslan Ö.; Parlak H.; Katalay S.; Boyacioglu M.; Karaaslan M.A.; Guner H.Micronuclei tests is a system of mutagenicity testing used for determining the pollution and chemicals causing changes in DNA fragments such as micronuclei in the cytoplasm of interphase cells. Damage caused on the DNA by genotoxic pollutants is the first consequence occurring in the aquatic organisms. Thus, it was attempted to determine whether pollution affected the erythrocytes and gills of fish Gobius niger and haemolymph and gills of mussels Mytilus galloprovincialis living in Izmir Bay at the level of DNA by the means of micronuclei (MN) test. Organisms used in the MN test were collected from seven locations (Alsancak, Alaybey Shipyard, Karsiyaka, Bostanli, Göztepe, Konak and Pasaport) which are known as the most polluted part of inner Bay of Izmir (Western Coast of Turkey). According to the results of the present study, frequency of MN was found at high level in Alaybey Shipyard and Pasaport where wastes from existing dockyard contributed to high level of pollution. In conclusion, this study indicates that the micronuclei test gives sensitive results in monitoring the pollution, especially the pollution of harbor, and thus it might be used as standard method in regular monitoring of pollution of coastal ecosystem. © 2009 Springer Science+Business Media B.V.Item Study of mutagenicity of metabolized pollutants in some tissues of black gobby (Gobius niger) and black mussels (Mytilus galloprovincialis) from izmir bay (Western Turkey)(2011) Boyacioglu M.; Parlak H.; Arslan O.C.; Katalay S.; Karaslan M.A.In the present study, fish and mussel samples from Izmir bay of Agean sea were studied for their mutagenic potential in TA98 and TAIOO strains of Salmonella typhimurium using Ames test (plate incorporation assay) without metabolic activation. Black gabby (Gobius niger) samples were collected from Bostanli and Pas aport locations and mussel (Mytilus galloprovincialis) samples were collected from Alsancak, Alsancak harbour, Alaybey Shipyard, Karsiyaka, Bostanli, Goztepe, Konak and Pasaport locations on Izmir bay. Extracts were obtained from hepatic and muscular tissues of black goby and whole soft parts of the mussels. Obtained extracts were studied for mutagenicity using the Ames test with S. typhimurium TA98 and TAIOO strains. According to results of the present study, mutagenicity was observed in liver extracts of the fish from Pasaport location whereas no mutagenicity was observed in liver and muscle extracts of fish from Bostanli location. Among the extracts of mussel samples collected from 8 locations only those from Alsancak Harbour were observed to possess weakly and directly mutagenic effects on S. typhimurium TA98 (frame shift mutations). © Medwell Journals, 2011.Item Histopathological alterations in gobius niger (black goby) due to pollution of the izmir bay(2012) Katalay S.; Minareci E.; Tuǧlu I.; Segner H.The present study aims to investigate the possible impact of aquatic pollution of Izmir Bay on resident fish. Gobius niger were sampled from two stations of the Izmir Bay, and gills and liver were examined histopathologically. The presence of pathological lesions in gills and liver of the fish as possible result of pollutant exposure was evaluated by semiquantitative analyses. The only histopathological changes found were hyperplasia, hypertrophy (12.5%, 18.8%), epithelial degeneration (18.8%, 25%) in gill and fatty livers (12.5%, 25%) of the fishes from both study sites, respectively. The moderate manifestation of pathological lesions in organs of Gobius niger from the presumably Bostanh site may be either the result of substantial protective and detoxification capabilities of this fish species, or from recent measures to reduce water pollution in Izmir Bay such as the installment of wastewater treatment plants.Item Phytotoxicity of water and sediment from Nif Brook (Izmir, Turkey) on green algae desmodesmus (=Scenedesmus) subspicatus; [Nif Çayı (İzmir, Türkiye) su ve sedimenti'nin yeşil alg desmodesmus (=Scenedesmus) subspicatus üzerine fitotoksisitesi](Cevkor Vakfi, 2012) Katalay S.; Boyacioglu M.; Cakal Arslan O.; Parlak H.; Ali Karaaslan M.The water of Nif Brook (Izmir, Turkey) is polluted by industrial, domestic, and agricultural sources. As the water of the brook is used for domestic and industrial water supply as well as for irrigation, it is of great importance to know the toxicity potential for the natural populations. For this purpose the standard test protocol for the short term phytotoxicity test method OECD 201 Algal Growth Inhibition has been evaluated with cultures of green algae Desmodesmus (=Scenedesmus) subspicatus as the representative of the first trophic level. The test has been used to assess the toxicity of both water and sediment samples from the brook. Five sampling sites from the brook were selected considering the type of pollution sources. The water samples were tested with 10, 20, 40, 60, 80 and 100 % dilutions in a test medium. Extracts of sediment samples were assayed in three different concentrations (20, 40 and 100 mg/mL). The effects on the growth of D. subspicatus were evaluated by scoring cell numbers under the light microscope with a Neubauer haemocytometer counting chamber. According to the results, the water samples stimulated the algal growth (except station 1); although all sediment samples inhibited the growth of populations, in several grades.Item Mutagenicity of sediment samples from Nif brook (Western Turkey)(Medwell Journals, 2012) Boyacioglu M.; Parlak H.; Arslan O.C.; Katalay S.; Karaslan M.A.The present study aimed to assess mutagenicity of the sediment samples from Nif brook using solvent extraction method and to compare its results with those of a previous study on mutagenicity using Soxhlet-extraction method. Sediment samples were collected from 5 sites located in Nif brook in April, 2007. Sediment samples from 5 sites on Nif brook were studied for their mutagenic potential on TA 98 and 100 strains of Salmonella typhimurium using Ames test (Plate incorporation assay) without metabolic activation. Extracts of the sediment samples were assayed in three different concentrations (In 25, 50, 100 ?g). Sediment sample from Nif 5 sides was observed to be weak-mutagenic and those from Nif 1 and 4 sides were observed to have toxic effects. Toxic effects of the sediment samples on bacteria were observed on the basis of significantly reduced numbers of revertants compared to the solvent controls. According to Ames criteria, sediment samples from the Nif 5 were considered as mutagenic on TA 98 strain. According to the present study, mutagenicity was detected in Nif brook by solvent extraction method. © Medwell Journals, 2012.Item Changes in the glutathione-s transferase activity of the mussel Mytilus galloprovincialis during exposure to bisphenol-A(Parlar Scientific Publications, 2014) Arslan O.C.; Parlak H.; Boyacioglu M.; Karaslan M.A.; Katalay S.Bisphenol A (BPA), is one of the most important industrial chemicals synthesized for diverse applications. The environmental concentrations of BPA are at high risk level due to use widely in many fields of industry according to the latest studies. In our study mussels (Mytilus galloprovincialis) were exposed to 50 μg/L of BPA and the changes of hepatic biomarker glutathione-s transferase (GST) activity were investigated. The results showed that the activity of GST was increased in BPA group (20.44 %) compared with control. Based on this experiment, we recommended that GST might be used as a biomarker of environmental pollution. © by PSP.Item Assessment of micronuclei induction in peripheral blood and gill cells of some fish species from Aliağa Bay Turkey(Elsevier Ltd, 2015) Çakal Arslan Ö.; Boyacioğlu M.; Parlak H.; Katalay S.; Karaaslan M.A.The aim of this paper was to assess the biological damages in fish caused by various mutagenic agents present in polluted waters of Aliağa Bay. For this purpose, micronuclei (MN) test was performed using peripheral erythrocytes and gill cells of different fish specimens caught from both polluted and relatively clean sites from Aliağa Bay (Turkey). Micronuclei tests is a system of mutagenicity testing used for determining changes in DNA fragments such as micronuclei in the cytoplasm of interphase cells caused by the pollution and chemicals in the environment. Thus, it was attempted to determine whether pollution affected the erythrocytes and gills of fish living in Aliağa Bay at the level of DNA by the means of micronuclei (MN) test. According to the results of present study, frequency of MN was found at high level in polluted site. In conclusion, this study indicates that the micronuclei test gives sensitive results in monitoring the pollution, especially the pollution of harbor, and thus it might be used as standard method in regularly monitoring pollution of coastal ecosystem. © 2015 Elsevier Ltd.Item Comparative study of heavy metals and selenium accumulation in the digestive gland and gills of Mytilus galloprovincialis (Lamarck, 1819) caught in Izmir Bay (Turkey); [İzmir Körfezi’nden (Türkiye) yakalanan Mytilus galloprovincialis (Lamarck, 1819)’un sindirim bezi ve solungaçlarındaki ağır metal ve selenyum içeriklerinin karşılaştırması](Turkish Biochemistry Society, 2015) Yabanlı M.; Katalay S.; Yozukmaz A.; İnanan B.E.Objective: The aim of this study was to evaluate heavy metals (Cr, Fe, Ni, Cu, Zn, As, Cd, Hg, Pb) and selenium levels in digestive gland and gill tissues of wild Mediterranean mussel Mytilus galloprovincialis (Lamarck, 1819) collected from four locations in Izmir Bay. Methods: A total of 180 samples of the wild Mediterranean mussel M. galloprovincialis with shell length of 50-60 mm were collected from four locations. Inductively coupled plasmamass spectroscopy was used to determine heavy metals and selenium concentrations in mussel samples after microwave digestion process. Results: The highest values (mg kg-1 dry weight) obtained for digestive gland and gills were 0.17 and 0.15 for Cr, 28.62 and 29.49 for Fe, 0.25 and 0.29 for Ni, 2.53 and 1.78 for Cu, 18.52 and 22.03 for Zn, 1.26 and 1.08 for As, 0.04 and 0.04 for Cd, 0.02 and 0.02 for Hg, 0.19 and 0.16 for Pb, 0.40 and 0.48 for Se, respectively. Statistically significant differences among digestive gland and gills (p<0.05) were found for Cr, Ni, Zn, As, Se and Cd. In general, the levels of the studied elements were higher for the Karşıyaka than for other three stations. Conclusion: The low levels of toxic metals (Cd, Hg, Pb, Cr, Ni) found in tissues of M. galloprovincialis, comparing with the other studies including taken place in the Inner of Izmir Bay. M. galloprovincialis can be used as a sensitive biomonitor for the availabilities of studied elements in the Inner Bay of Izmir, Turkey. © TurkJBiochem.com.Item Histopathological and apoptotic changes on marine mussels Mytilus galloprovincialis (Lamark, 1819) following exposure to environmental pollutants(Elsevier Inc., 2016) Yavaşoğlu A.; Özkan D.; Güner A.; Katalay S.; Oltulu F.; Yavaşoğlu N.Marine bivalve mussels, especially Mytilus species are an earlywarning system used for determining of damage caused by the various aquatic pollutions. In the present study, Mytilus galloprovincialis L. (black mussel) have been utilised as a biomonitoring organism to reveal environmental pollution in the Aliaga, Foca and Urla where located along the Izmir Coast of Turkey. Mussels were collected at these areas and gill and hepatopancreas (digestive gland) tissues were excised. mRNA expressions of initiator (caspase-2 and − 8) and executioner (caspase − 3/7–1, − 3/7–2, − 3/7–3 and − 3/7–4) caspases of mussels tissues in areas exposed to pollution agent have been observed. TUNEL immunoreactivity in paralel to histopathological changes in both Aliaga and Foca areas were compared with Urla. This study is the first report to reveal the pollution with apoptotic expression on mussels in the coast of Turkey. © 2016 Elsevier LtdItem Multi-Biomarker Responses After Exposure to Pollution in the Mediterranean Mussels (Mytilus galloprovincialis L.) in the Aegean Coast of Turkey(Springer New York LLC, 2017) Ozkan D.; Dagdeviren M.; Katalay S.; Guner A.; Yavaşoğlu N.Ü.K.In this study, sublethal effects on the Mediterranean mussels (Mytilus galloprovincialis L.) collected from the Aegean coast of Turkey were determined. Enzymes such as glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), and acetylcholinesterase (AChE), metallothionein (MT) mRNA expressions, thiobarbituric acid reactive substances (TBARS) contents, determination of 14 heavy metals and micronucleus frequency were selected as multibiomarkers. Results show that heavy metals and an increase in the level of MT gene expression have been determined in tissues of mussels collected from all stations. The GST, SOD and CAT enzymes were increased in mussels of Aliaga and Old Foca, compared to the mussels of Urla, while it was showed inhibition at AChE levels. Extensive LP is determined on mussels of Aliaga. It was determined that mussels in Aliaga region have exposed more oxidative stress than Old Foca and Urla. These biomarkers were carried out for the first time in these stations to assess environmental quality. © 2016, Springer Science+Business Media New York.Item Characterization of hemocytes from different body fluids of the eastern oyster Crassostrea virginica(Academic Press, 2017) Lau Y.-T.; Sussman L.; Pales Espinosa E.; Katalay S.; Allam B.Bivalve hemocytes are involved in a variety of physiological and immunological functions. Circulating hemocytes in the hemolymph represent the main component of the internal self-defense system while hemocytes present in the extrapallial space (between the mantle and the shell) are actively involved in biomineralization and shell formation. This study focused on the characterization of hemocytes from different body fluids of the eastern oyster Crassostrea virginica. Hemocytes present in the hemolymph were compared to those contained in the extrapallial fluid. Hemocytes associated with the mucus layer covering pallial organs (mantle, gills, body wall) were also investigated because of their potential role as sentinel cells. Hemocytes were characterized using flow cytometry in conjunction with fluorescent epitope markers (clusters of differentiation, lectins) as well as functional assays (i.e. phagocytosis and reactive oxygen species -ROS). Compared with the hemolymph, there was a significantly greater percentage of granulocytes and agranulocytes among extrapallial and pallial hemocytes, respectively. Accounting for the different percentages of hemocyte sub-populations, significant differences in surface carbohydrate and clusters of differentiation signatures were also revealed between the different fluids. Most informative epitope markers included concanavalin A, peanut agglutinin, soybean agglutinin, CD11b and CD14. Functional assays revealed significant differences in phagocytic activity and ROS production between hemocytes from the extrapallial fluid and hemolymph; however, less robust differences were observed between hemolymph cells and hemocytes associated with the pallial mucus. Findings from this study suggest that there are markedly different hemocyte populations in the three body fluids. The role of peripheral cells, particularly those associated with the pallial mucus, requires further investigations. © 2017 Elsevier LtdItem An investigation on the seasonal variations of the biomarkers of oxidative stress response and their correlations to Polonium-210 in mussel (Mytilus galloprovincialis) and common sole (Solea solea) from İzmir Bay, Turkey(Elsevier Ltd, 2018) Aslan E.; Uğur Görgün A.; Katalay S.; Filizok I.; Becerik S.; Aydemir T.It is well known that the marine organisms are used as biological indicators for environmental pollution studies. Among these studies, the research on oxidative stress has been increasing in recent years. In this study, mussels (Mytilus galloprovincialis) and fish (Solea solea) samples were collected seasonally from İnciraltı İzmir, Turkey. This station was in an area where fishing is carried out for human consumption. The relationship between 210Po and oxidative stress markers (lipid peroxidation (LPO), H2O2 and proline) was investigated in the mussel tissue (digestive gland, gills) and fish tissue (liver, gills) samples. The present study indicated that H2O2 accumulated with increasing 210Po concentration in mussel samples. Statistically significant correlation were found between H2O2 and 210Po and LPO and proline in mussel samples. This correlation between LPO and proline can be attributed to common environmental parameters (other than 210Po) affecting expression of both LPO and proline levels. There was not a significant correlation between 210Po and LPO levels. Similarly, a significant correlation was not found between 210Po and proline. © 2018 Elsevier LtdItem How pollution effects the immune systems of invertebrate organisms (Mytilus galloprovincialis Lamark, 1819)(Elsevier Ltd, 2021) Ayhan M.M.; Katalay S.; Günal A.Ç.Hemocytes are the main immunologic parameters for invertebrate organisms as a response to diseases and pollutions. This study was aimed to reveal the relations between pollution and the total and differential hemocytes numbers. The gulf of İzmir was selected as location for sampling due to its contaminated by different anthropogenic pollutants mainly industrial effects. The effects of the pollutants were investigated on the mussels that have been collected from eight stations in the Izmir Bay (1- Inciralti, 2- Göztepe 3-Konak-4- Pasaport 5-Alsancak, 6-Karşıyaka, 7-Bostanlı), which are known as the most polluted part of inner Bay of Izmir (Western Coast of Turkey) and 8-Foça, (an anthropogenically industrialized and shipping -impacted area). The pollution effects in the bay were determined on hemolymphs by biomarkers such as total hemocyte counts (THCs) and differential hemocyte counts (DHCs). Microscopic examinations have shown the statistical differences at THCs and DHCs. The highest total hemocyte number was determined in the station 7 (Bostanlı) and the lowest in the station 4 (Pasaport) (P < 0.05). The number of total hemocyte counts were not differed significantly in the Stations among 2 (Göztepe), 5 (Alsancak) and 6 (Karşıyaka), and between Stations 8 (Foça) and 1 (Inciraltı) (P > 0.05). In addition, there have been significant differences in subpopulation according to differential hemocyte numbers. The results indicate that the station 8 (reference site-Foça) has the highest agranulocyte number while station 7 (Bostanlı) and the station 1 (İnciraltı) have the lowest agranulocyte numbers (P < 0.05). As for the basophilic hemocyte numbers, this situation was reversed highest number in station 1 (Inciraltı) and 7 (Bostanlı) and lowest in the station 8 (Foça) (p < 0.05). The station 4 (Pasaport) has the highest eosinophilic hemocyte number while station 8 (Foça) has the lowest. We concluded that differential hemocytes counts are more sensitive as biomarker with the highest numbers of agranulocytes and lowest numbers of eosinophilic and basophilic hemocytes in reference site. © 2021Item Cellular Biomarkers in Mytilus galloprovincialis L. (Mediterranean Mussels) from Izmir Bay (Turkey)(Springer, 2022) Katalay S.; Ayhan M.M.; Guler C.; Aldag C.; Kilic T.; Yavasoglu N.U.K.In this study, cellular biomarkers and heavy metal concentration in the Mytilus galloprovincialis L. (Mediterranean mussels) collected from eight sites of Izmir Bay (Turkey) were determined to reveal water pollution for the first time in these stations. Results show that heavy metals (As, Cu, Hg, Zn, Cd, Sn, Pb) have been specified in mussels’ tissues collected from all stations. According to GST, SOD, CAT activities, and TBARS contents, mussels in the outer bay have exposed more oxidative stress than the ones in the inner bay. Digestive gland tissues of them were showed more inhibition at AChE levels than gills. Also, abnormal nucleus rates and micronucleus frequencies (MN) were found to be higher in the inner bay than in the outer bay. This study showed that heavy metal pollution in different levels is an environmental issue on the Izmir Bay. Especially the coastal regions of the bay have been extremely affected by anthropogenic effects due to growing population. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.Item Oxidative stress-induced apoptotic changes after acute exposure to antifouling agent zinc pyrithione (ZnPT) in Mytilus galloprovincialis Lamark (Mediterranean mussels) tissues(Taylor and Francis Ltd., 2022) Katalay S.; Guner A.; Dagdeviren M.; Yigitturk G.; Yavasoglu A.; Gunal A.C.; Karabay Yavasoglu N.U.; Oltulu F.Zinc pyrithione (ZnPT) is one of the components used in antifouling paints and can be an alternative to classical toxic chemicals such as organotin. However, there is still remarkable concern about the environmental safeness of ZnPT due to rapid transchelation and degradation into several metabolites that have their own toxicity. The effect after acute exposure of ZnPT is investigated on Mediterranean mussels exposed to 20 and 40 μg/L concentrations for 48 and 96 h and antioxidant responses [superoxide dismutase (SOD), and reduced glutathione (GSH)], genotoxicity [micronuclei (MN) frequency], apoptotic and histological changes were determined. Severe histological changes in hepatopancreas and gill tissues of mussels were observed in ZnPT exposed groups due to dose-dependent increase. ZnPT also caused a dose-dependent increase of TUNEL-positive cell count in the mussel tissues, especially in the hepatopancreas. Increasing in SOD activities and decreasing in GSH levels in both ZnPT concentrations compared to the control were observed. MN and binuclei numbers in all exposure groups were significantly increased. The results of the present study demonstrate that acute exposure to ZnPT could cause an adverse effect on mussel tissues at especially higher concentrations. © 2022 Informa UK Limited, trading as Taylor & Francis Group.Item Combined evaluation of multi-biomarkers and metal bioaccumulations in two different fish species (Sparus aurata and Chelon labrosus) from İzmir Bay, Türkiye (Aegean Sea): Spatial, temporal and tissue-specific approaches(Elsevier Ltd, 2023) Bilgin M.; Uluturhan E.; Darilmaz E.; Katalay S.Biomarkers were investigated to assess the effects of metal accumulation in Sparus aurata and Chelon labrosus in the İzmir Bay. Fish were collected from the Inner and Outer Bays in November 2020 and May 2021. According to the metal and biomarker measurements found in the organs, it was observed that the levels were higher in the liver and gill tissues than in the muscle tissues. Significant differences between tissues were found for all metals and biomarkers. In addition, biochemical biomarkers were found to be significant predictors of metal bioaccumulation. Histological changes were observed in liver and gills in each species and location. Although the metal levels determined according to the health risk assessment were below the threshold limits, the As levels for the lifetime cancer risk were within the limits to be considered. In conclusion, this study will provide robust results for its related study area. © 2023 Elsevier Ltd