Browsing by Author "Kendirci, R"

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    Cell cycle controlling of cancer stem cells in primary and metastatic colon carcinoma
    Kurt, FO; Kendirci, R; Türkoglu, C; Vatansever, S
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    In Vitro Cultivation, Characterization and Osteogenic Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth on 3D Printed Polylactic Acid Scaffolds
    Islam, A; Mammadov, E; Kendirci, R; Aytac, E; Cetiner, S; Vatansever, HS
    Background: Tissue engineering mainly focuses on creating appropriate conditions for the regeneration of tissues. Scaffolds, signal molecules, and stem cells interact with each other and compose the essential components of this field. Objectives: This study aimed at investigating the osteogenic induction ability of PLA Poly Lactic Acid (PLA) scaffolds and comparing the osteogenic differentiation behavior of Stem Cells from Human Exfoliated Deciduous Teeth (hSHEDs) in standard culture medium and on PLA scaffolds. Methods: The current clinical experimental study was conducted between April 2016 and October 2016 at the Near East University cell culture laboratory located in North Cyprus. The pulp tissues of deciduous teeth (non-decayed and in the absence of abscess, fistula or periapical lesion) were sampled from 10 healthy children aged between 6 and 11 years. The isolated hSHEDs were divided to 4 groups. The control group/Group1 consisted of cells, which were cultivated in standard culture medium, and Group2 cells were differentiated into an osteogenic lineage using osteogenic differentiation medium. Group 3 represented the non-differentiated group, which was transferred onto three dimensional (3D) printed PLA scaffolds and Group 4 cells were differentiated to the osteogenic lineage and transferred onto 3D printed PLA scaffolds. All groups were analyzed immunohistochemically and by immune-labeling, and were evaluated semi-quantitatively using the HSCORE. Results: Cultivation of hSHEDS on PLA scaffolds was assessed for 14 and 21 days; osteogenic differentiation was detected both histochemically and immunohistochemically. Generally, Osteocalcin (OCN) immunoreactivities were higher than Osteonectin (ON) immunoreactions in all groups. Despite higher OCN immunoreactivities, the intensities of OCN between 14 days and 21 days in group 4 (497.3 +/- 0.57% and 486.7 +/- 5.77%, respectively) were similar (P > 0.05). While the intensity of ON was 280.0 +/- 10% in group 4, in group 2 the intensity of ON was 206.7 +/- 5.77%, and on the 14th day the results were statistically significant (P < 0.0001). Conclusions: Poly lactic acid is a suitable scaffold material for osteogenic induction of the hSHEDs. The expression patterns of both markers showed that a 14-day cultivation period is adequate for hSHEDs with/without PLA scaffolds to differentiate into osteoblasts.
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    Synthesis and cytotoxic activities of organometallic Ru(II) diamine complexes
    Kavukcu, SB; Sahin, O; Vatansever, HS; Kurt, FO; Korkmaz, M; Kendirci, R; Pelit, L; Türkmen, H
    A series of mono and bimetallic ruthenium(II) arene complexes bearing diamine (Ru1-6) were prepared and fully characterized by H-1, C-13, F-19, and P-31 NMR spectroscopy and elemental analysis. The crystal structure of the bimetallic complex (Ru-5) was determined by X-ray crystallography. Monometallic analogues (Ru1-3) were synthesized to investigate the contributions of ruthenium and the other organic groups (aren, ethylenediamine, butyl) to the activity. The electrochemical behaviors of mono and bimetallic complexes were obtained from the relationship between cyclic voltammetry (CV) and the biological activities of the compounds. The cytotoxic activities of the complexes (Ru1-6) were tested against wide-scale cancer cell lines, namely HeLa, MDA-MB-231, DU-145, LNCaP, Hep-G2, Saos-2, PC-3, and MCF-7, and normal cell lines 3T3-L1 and Vero. Diamine Ru(II) arene complexes have unique biological characteristics and they are promising models for new anticancer drug development. MTT analysis reveals that each synthesized Ru complex showed cytotoxic activity towards the different cancer cells. In particular, three Ru complexes (Ru-3, Ru-5 and Ru-6) showed less toxic effects on the cancer cells than the others. These novel Ru complexes affected both cancer and normal cell lines. As they had a toxic effect on the cells, the dosage applied should be tested before being used for in vivo applications. Cytotoxicity tests have shown that the bimetallic complex Ru-6 was effective on all cancer cells. The effect of bimetallic enhancement on cancer cell lines, the systematic variation of the intermetallic distance and the ligand donor properties of the mono and bimetallic complexes were explored based on the cytotoxic activity. The interaction with FS-DNA and the stability/aquation of the complexes (Ru-3 and Ru-6) were investigated with H-1 NMR spectroscopy. The binding modes between the complexes (Ru-3 and Ru-6) and DNA were investigated via UV-Vis spectroscopy.
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    Effects of adipose and bone marrow-derived mesenchymal stem cells on vaginal atrophy in a rat menopause model
    Kasap, B; Kasap, S; Vatansever, S; Kendirci, R; Yilmaz, O; Çalisir, M; Edgünlü, T; Akin, MN
    Background & objectives: Vaginal atrophy is characterized by thinning of vaginal epithelial layers and decreased local blood flow. We aimed to evaluate the regenerative effects of Adipose derived mesenchymal stem cells (ADMSC) and Bone marrow derived mesenchymal stem cells (BMDSC) on vaginal atrophy in rat menopause model. Materials and methods: Rats were randomly divided into 4 (four) groups: sham, control, ADMSC, BMDSC. Vaginal epithelial thickness, structure of the lamina propria, blood vessels in the lamina propria, collagen deposition, and muscle structure were evaluated. Anti ER alpha, VEGF, VEGFR 1, Bax and bcl-2 antibodies were analyzed. Beta actin gene was used as endogenous control. Genetical differences among the groups were compared by using Kruskal Wallis and Mann Whitney U test. p < 0.05 was regarded as statistically significant. Results: Epithelial thickness of ADMSC group was higher than control group, but less than sham group Epithelial thickness of BMDSC group was less than sham group. Lamina propria and muscle tissue of ADMSC and BMDSC groups were found to be similar to sham group. VEGFR-1, VEGF, Bax and ER-a staining levels were higher in ADMSC and BMDSC groups than control group. ADMSC group stained stronger with VEGFR-1 and VEGF than BMDSC group. Bcl-2 staining level was increased in ADMSC applied group. No statistically significant difference was detected in Bax and Bcl-2 genes and Bax-/Bcl-2 ratio. Conclusions: Although genetic expression might have ended and could not be significantly demonstrated, histological and immunohistochemical results favor ADMSC application in vaginal atrophy rather than BMDSC.
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    Proapoptotic and Anticancer Potentials of Thymus capitatus Essential Oil on Colon Cancer Stem Cells
    Yavuz, DÖ; Becer, E; Kendirci, R; Kurt, FÖ; Vatansever, HS
    BACKGROUND/AIMS The aim of this study was to investigate the proapoptotic activity of Thymus capitatus essential oil in either colon cancer stem (CDI33+ Colo-320) or nonstem (CDI33- Colo-320) cells. MATERIAL and METHODS T. capitatus essential oil was obtained by water distillation and analyzed by GC-MS. Cancer stem cells (CDI33+ Colo-320) were obtained from the Colo-320 cells by the MiniMACS system. Proapoptotic activity of T capitatus essential oil was investigated by immunocytochemistry using antibodies directed against caspase-3 and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. RESULTS Caspase-3 immunoreactivity was significantly increased in 0.5% dilution T. capitatus essential oil-treated Colo-320 cells for 48 hours Moreover, the number of TUNEL positive cells was significantly higher in Colo-320 cells when compared with CDI33+ and CDI33- Colo320 cells. CONCLUSION We conclude that T capitatus essential oil increases caspase-3 molecules, which play a crucial role in apoptosis. Interestingly, T capita- tus essential oil is found to be more effective in Colo-320 cells than CDI33+ Colo-320 and CDI33- Colo-320 cells in terms of apoptosis.