Browsing by Author "Koçbaş F."
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Item Metal bioaccumulation by barley in mesaoria plain alongside the nicosia-famagusta highway, Northern Cyprus(2009) Gücel S.; Koçbaş F.; Öztürk M.Mesaoria plain is mainly used for agricultural purposes and contains 50 % of the barley fields in Northern Cyprus. The construction of a highway from Nicosia to Famagusta that passes through the plain has resulted in an increasing number of vehicles from 1997 to 2006, the majority of which run on leaded gasoline. Barley is cultivated on a large scale along the Nicosia-Famagusta highway, and was tested as a possible biomonitor for heavy metals. During the vegetative period, samples of barley plants and soil were collected at 4 distances (0, 50, 100 and 500 m) from the highway, at 4 sites, from January to April 2006. The highest concentrations of Ph++ (2.82 μg g -1), Cd++" (1.52 μg g-1), Cr ++(2.86 μg g.1), Zn++(51.8 μg g -1) and Cu++(19.8 μg g-1) were recorded in soil collected beneath the highway, and the lowest in samples collected at 500 m from the highway, for Pb++ (0.54 μg g-1) in the above-ground parts of barley, Cr++ (0.85 μg g-1) in soil, and Zn++ (7.2 μg g-1) in barley plant roots. A positive relationship was found between soil and plant heavy metal concentrations. According to the EU Directive 86/278/EEC on the protection of the environment and, in particular, of the soil, concentrations of Pb44" and Zn++ are below the threshold, Cd++ are between the limits and Cr++ are above threshold.Item The seasonal distribution of heavy metals in Mussel sample from Yalova in the Marmara Sea, 2008-2009(2011) Türk Çulha S.; Koçbaş F.; Gündocdu A.; Baki B.; Çulha M.; Topçuoclu S.Concentration of the some heavy metals (Co, Cu, Fe, Ni, Pb and Zn) Mediterranean mussel Mytilus galloprovincialis from Yalova (Marmara Sea) were investigated seasonally. The mean concentrations of Co, Cu, Fe, Ni, Pb and Zn in the soft tissues were 4.08 ± 0.67, 5.54 ± 0.59, 156.72 ± 20.18, 3.71 ± 1.14, 2.92 ± 0.51 and 106.23 ± 5.66 mg kg -1 dry weights, respectively. There were no significant differences between the metal concentrations among the seasons (P> 0.05). The mean Pb and Zn concentrations were higher than the acceptable limit proposed by International Turkish standards. © 2011 Springer Science+Business Media B.V.Item Daphnia magna as a test species for toxicity evaluation of municipal wastewater treatment plant effluents on freshwater cladoceran in turkey(Central Fisheries Research Inst, 2015) Koçbaş F.; Oral R.Aquatic toxicity of municipal wastewater was evaluated in an acute toxicity test using water flea, Daphnia magna as an freshwater aquatic experimental animal model. Toxicity test were performed on samples of both untreated (raw) and treated wastewaters were collected Manisa municipal effluents. Undiluted untread and treated effluents were very toxic to D. magnaand cause to death of all exposed daphnids. Dilution of wastewaters was observed to decrease percentage of influence of biological toxicity based on dilutional rate. Acute toxic effect of untreated wastewater on D. magnawas more than that of treated wastewater. In addition, the longer the period of exposure to D. magna, the more significantly toxic effect increased. © Published by Central Fisheries Research Institute (CFRI) Trabzon.Item Comparative toxicities of selected rare earth elements: Sea urchin embryogenesis and fertilization damage with redox and cytogenetic effects(Academic Press Inc., 2016) Pagano G.; Guida M.; Siciliano A.; Oral R.; Koçbaş F.; Palumbo A.; Castellano I.; Migliaccio O.; Thomas P.J.; Trifuoggi M.Background: Broad-ranging adverse effects are known for rare earth elements (REE), yet only a few studies tested the toxicity of several REE, prompting studies focusing on multi-parameter REE toxicity. Methods: Trichloride salts of Y, La, Ce, Nd, Sm, Eu and Gd were tested in Paracentrotus lividus sea urchin embryos and sperm for: (1) developmental defects in either REE-exposed larvae or in the offspring of REE-exposed sperm; (2) fertilization success; (3) mitotic anomalies in REE-exposed embryos and in the offspring of REE-exposed sperm, and (4) reactive oxygen species (ROS) formation, and malondialdehyde (MDA) and nitric oxide (NO) levels. Results: REEs affected P. lividus larvae with concentration-related increase in developmental defects, 10-6 to 10-4 M, ranking as: Gd(III)>Y(III)>La(III)>Nd(III)≅Eu(III)>Ce(III)≅Sm(III). Nominal concentrations of REE salts were confirmed by inductively coupled plasma mass spectrometry (ICP-MS). Significant increases in MDA levels, ROS formation, and NO levels were found in REE-exposed embryos. Sperm exposure to REEs (10-5 to 10-4 M) resulted in concentration-related decrease in fertilization success along with increase in offspring damage. Decreased mitotic activity and increased aberration rates were detected in REE-exposed embryos and in the offspring of REE-exposed sperm. Conclusion: REE-associated toxicity affecting embryogenesis, fertilization, cytogenetic and redox endpoints showed different activities of tested REEs. Damage to early life stages, along with redox and cytogenetic anomalies should be the focus of future REE toxicity studies. © 2016 Elsevier Inc.Item Comparative multi-species analysis of potassium cyanide toxicity(Elsevier Ltd, 2022) Tez S.; Oral R.; Koçbaş F.; Koru E.; Türkçü N.; Pagano G.; Trifuoggi M.Potassium cyanide (KCN), a highly water soluble and bioaccumulative cyanide salt, is examined to determine the toxic effects by using two green algae (Dunaliella viridis, Nannochloropsis oculata) and genetically different two sea urchin (Paracentrotus lividus, Arbacia lixula) species. To determine the toxic effects on the early developmental stages of sea urchin embryos, 72-hour embryotoxicity studies were conducted. Potassium cyanide toxicity at cellular level was also investigated and 6-hour embryos of both sea urchin species were used to determine genotoxic effects of KCN. Since plutei naturally feed on microalgae, two species of plankton were used to reveal phytotoxic effects of KCN. KCN was found to be embryo- geno- and phytotoxic. EC50's for P. lividus and A. lixula were found 7.96 and 6.52 μM. IC50's for N. oculata for 48 h and 72 h were found 23.66 and 80.45 μM. IC50's for D. viridis for 48 h and 72 h were found 14.31 and 23.36 μM. © 2022 Elsevier Ltd