Browsing by Author "Kocyigit A."

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    Decolorization of various leather dyes and leather industry effluent by Trametes trogii TEM H2
    (2012) Pazarbasi M.B.; Kocyigit A.; Ozdemir G.; Yasa I.; Karaboz I.
    Decolorization of Acid Blue 7 which is used widely in leather industry was investigated as a model for a decolorization system using soluble starch yeast extract medium under agitated and static conditions with Trametes trogii TEM H2. The effects of different physico-chemical parameters were tested and optimal decolorization rates occurred at pH 5.0 and at 27°C. Decolorization of Acid Blue 7 under agitated and static conditions was determined to be 99.9% and 63.5%, respectively. Decolorization was associated with lacease activity which reached 1110.3 U/L in agitated cultures in the presence of Acid Blue 7 on the 6th day of cultivation. T. trogii TEM H2 was further evaluated for the decolorization of 8 other leather dyes, such as Acid Black 210, Acid Green 20, Acid Yellow 36, Acid Black 24, Acid Black 234, Acid Violet 17, Acid Blue 134, Acid Brown 349, and a mixture of Acid Blue 7 with these 8 leather dyes and leather industry effluents. The decolorization rates after 24 h for the dye mixture and the effluent (10%) were 88% and 48%, respectively. The strain was considered as a good candidate for biodegradation and bioremediation of leather dye-polluted effluents due to its lacease production and decolorizing ability. © by PSP.
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    Production of laccase from Trametes trogii TEM H2: A newly isolated white-rot fungus by air sampling
    (2012) Kocyigit A.; Pazarbasi M.B.; Yasa I.; Ozdemir G.; Karaboz I.
    This work represents the first report of isolation of potential laccase producers by air sampling using media supplemented with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) and guaiacol for laccase production and secretion indicators. Nine fungal isolates showed positive reactions with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) and guaiacol. The isolate named TEM H2 exhibited the largest and intensive oxidation zones with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) (85 mm) and guaiacol (66 mm) and therefore it was selected for detailed investigations. The strain was identified as Trametes trogii TEM H2 due to the morphological characteristics and the comparison of internal transcribed spacer ribosomal DNA gene sequences. The laccase production was screened in different liquid cultures. The best laccase production medium was determined as soluble starch yeast extract medium in which laccase production was reached to a maximum level (989.6 U l-1) on the 8th day of cultivation. Effects of different initial pH values on laccase production were tested. Optimum pH value for laccase production in soluble starch yeast extract medium was determined as pH 3.0 with 15425.0 U l-1laccase production at 12th day of cultivation. In addition, effects of eight inducers (veratryl alcohol, ferulic acid, 1-Hydroxybenzotriazole, syringic acid, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate), 1 mmol l-1 CuSO4, 3% ethanol, guaiacol) were examined. Only cultures with 2,5-xylidine exhibited 1.9 fold increase in laccase activity reaching to 28890.0 U l-1. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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    Evaluation of xenon, light-emitting diode (LED) and halogen light toxicity on cultured retinal pigment epithelial cells
    (Taylor and Francis Ltd, 2019) Sezer T.; Altinisik M.; Guler E.M.; Kocyigit A.; Ozdemir H.; Koytak A.
    Objective: To compare the possible toxic effects of three light sources used in vitreoretinal endoillumination systems; halogen, xenon, and light-emitting diode (LED) on retinal pigment epithelium (RPE) cell cultures, after two different exposure times. Material and methods: ARPE-19 human RPE cell cultures were exposed to halogen, xenon, and LED light sources at a distance of 1.5 cm for 30 and 60 min with equal lumen output levels. Cells in the control group were not exposed. RPE cell cultures were compared in terms of cell viability, DNA damage, apoptosis rate, and IL-1ß, IL-6, and TNF- α levels. Results: The halogen light group showed significantly more DNA damage, higher TNF-α, IL-1β, and IL-6 levels, and lower viable cell count at 30 min compared to the control group. The rates of early and late apoptosis were also significantly higher at 60 min. There were no statistically significant differences in any of the parameters between the xenon and LED light sources and the control group at 30 or 60 min. Conclusion: New generation lights, xenon, and LED, seem to be safe in terms of RPE cells. Halogen light may cause toxic effects on RPE cells when used for a long time with maximal power output. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.