Browsing by Author "Kolatan, E"

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    Correlation Between Intestinal Colony Numbers and Inflammation Markers (TNF-Alpha, IL-6, High-Sensitive CRP) Following the Use of Sevelamer in Rats with Kidney Failure
    Kürsat, S; Çolak, H; Yilmaz, O; Kolatan, E; Ulman, C; Kurutepe, S; Özyurt, B
    Objective: Our aim was to investigate anti-inflammatory effect of sevelamer in experimentally induced renal failure (RF) and whether this effect was related to a decrease in Escherichia coli colony counts in feces. Material and Methods: Eighteen female Wistar albino rats weighted 200-250 g were divided into three groups. RF was induced by 5/6 nephrectomy. Group 1 was the control group. Group 2 rats were those with induced RF receiving a high phosphate diet. Group 3 contained rats with induced RF receiving a high phosphate diet + sevelamer. E.coli count in feces, plasma creatinine, high-sensitive C-reactive protein (hsCRP), interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) concentrations were determined at the beginning and at the end of 1st, 3rd and 6th weeks in all rats. Results: In Group 2 and 3, creatinine concentrations were found to be increased (p=0.03, p=0.02) in the 1st week. In Group 2, there was an increase in number of E. coli colony count in the 3rd week (p=0.05). In group 3, E. coli colony count and levels of inflammatory markers (IL-6, CRP, TNF-alfa) significantly were increased in comparison to the basal :+alues (p=0.042, p=0.021, p=0.042, respectively) in the 1st week. In group 3, in the sixth week, E. coli colony count (p=0.02) and levels of inflammatory markers (IL-6, hsCRP, TNFalpha) (p<0.05) were found to be significantly decreased in comparison to the 1st week levels. Conclusion: Anti-inflammatory effects of sevelamer might be explained by its effects on the bacterial colonization in colon.
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    Effect of Erythropoietin on Acoustically Traumatized Rat Cochlea An Immunohistochemical Study
    Gürgen, O; Gürgen, SG; Kirkim, G; Kolatan, E; Gürkan, S; Güvenç, Y; Eskiizmir, G
    OBJECTIVE: To investigate the audiological and histopathological effects of erythropoietin on acoustic over-stimulation in rats. STUDY DESIGN: Twenty-two male Wistar albino rats were divided into 3 groups: sham group (n = 7), erythropoietin injection group (n = 8), and saline injection group (n = 7). Both erythropoietin and saline injection groups were exposed to white noise (100 decibel [dB] sound pressure level (SPLD for 3 hours. Auditory brainstem responses were measured before, immediately after, and on the 7th day of noise exposure. All animals were sacrificed on the 7th day and temporal bones were collected. The serial sections of the cochleae were stained by caspase-3 and caspase-9 immunostaining and by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method in order to detect apoptotic cells. RESULTS: In the saline group statistically significant differences were detected between the baseline and immediate postacoustic overstimulation thresholds of click and 6 kHz stimuli. However, when the baseline and immediate postacoustic overstimulation thresholds of click and 6 kHz stimuli were compared in the erythropoietin injection group, no statistically significant difference was determined. Histo pathologic evaluations demonstrated that erythropoietin decreased the amount of apoptotic cells in the cochlea. CONCLUSION: Erythropoietin is likely to prevent the acute threshold changes and decrease the amount of apoptosis in cochlea after acoustic overstimulation in rats.