Browsing by Author "Korkmaz, E"

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    Endoplasmic Reticulum Stress-Induced Apoptotic Effects of Novel 1-Pyrroline (3,4-Dihydro-2H-pyrrole) Derivatives on Breast Cancer Cells
    Atmaca, H; Ilhan, S; Korkmaz, E; Zora, M
    Heterocyclic compounds have emerged as promising and appealing scaffolds for developing effective antitumor agents. Here, the effects of synthesized 24 different 1-pyrroline derivatives (PDs) containing substituted aryl sulfide moiety were investigated on human breast cancer cell lines. The viability of cells was assessed via MTT assay. Reactive oxygen species (ROS) generation was analyzed via fluorescent dye CM-H2DCFDA. Apoptotic cells were determined via flow cytometry. Endoplasmic reticulum (ER) stress-associated protein levels were analyzed via western blot analysis. Four of the PDs (PD-12, -14, -16 and -17) had great cytotoxic selectivity against breast cancer cells. Apoptotic cell death was induced by PDs via the generation of ROS. PDs significantly increased the GRP78, p-PEAK, p-eIF2 alpha, and CHOP protein levels indicating ER stress in breast cancer cells. These results imply that newly synthesized PDs may be potential anticancer agents as they selectively inhibit breast cancer cells.
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    N-Propargylic β-enaminones in breast cancer cells: Cytotoxicity, apoptosis, and cell cycle analyses
    Ilhan, S; Atmaca, H; Yilmaz, ES; Korkmaz, E; Zora, M
    Breast cancer is one of the most common cancers worldwide and the discovery of new cytotoxic agents is needed. Enaminones are regarded to be a significant structural motif that is found in a variety of pharmacologically active compounds however the number of studies investigating the anticancer activities of N-propargylic beta-enaminones (NPEs) is limited. Herein we investigated the potential cytotoxic and apoptotic effects of 23 different NPEs (1-23) on human breast cancer cells. Cytotoxicity was evaluated via MTT assay. Apoptotic cell death and cell cycle distributions were investigated by flow cytometry. CM-H2DCFDA dye was used to evaluate cellular ROS levels. Expression levels of Bcl-2, Bax, p21, and Cyclin D1 were measured by quantitative real-time PCR. ADME properties were calculated using the ADMET 2.0 tool. NPEs 4, 9, 16, and 21 showed selective cytotoxic activity against breast cancer cells with SI values > 2. NPEs induced apoptosis and caused significant changes in Bcl-2 and Bax mRNA levels. The cell cycle was arrested at the G0/G1 phase and levels of p21 and Cyclin D1 were upregulated in both breast cancer cells. ROS levels were significantly increased by NPEs, suggesting that the cytotoxic and apoptotic effects of NPEs were mediated by ROS. ADME analysis revealed that NPEs showed favorable distributions in both breast cancer cell lines, meaning good lipophilicity values, low unfractionated values, and high bioavailability. Therefore, these potential anticancer compounds should be further validated by in vivo studies for their appropriate function in human health with a safety profile, and a comprehensive drug interaction study should be performed.
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    Lacunary statistical convergence on L-fuzzy normed space
    Yapali, R; Korkmaz, E; Çinar, M; Çoskun, H
    The idea of lacunary statistical convergence sequences, which is a development of statistical convergence, is examined and expanded in this study on L-fuzzy normed spaces, which is a generalization of fuzzy spaces. On L-fuzzy normed spaces, the definitions of lacunary statistical Cauchy and completeness, as well as associated theorems, are provided. The link between lacunary statistical Cauchyness and lacunary statistical boundedness with regard to L-fuzzy norm is also shown.
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    Patterns of care for lung cancer in radiation oncology departments of Turkey
    Demiral, A; Alicikus, ZA; Ugur, V; Karadogan, I; Yoney, A; Andrieu, MN; Yalman, D; Pak, Y; Aksu, G; Ozyigit, G; Ozkan, L; Kilciksiz, S; Koca, S; Caloglu, M; Yavuz, A; Caglar, H; Beyzad-eoglu, M; Igdem, S; Serin, M; Kaplan, B; Koc, M; Korkmaz, E; Celik, OK; Kinay, M
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    PATTERNS OF CARE FOR LUNG CANCER IN RADIATION ONCOLOGY DEPARTMENTS OF TURKEY
    Demiral, AN; Alicikus, ZA; Ugur, VI; Karadogan, I; Yöney, A; Andrieu, MN; Yalman, D; Pak, Y; Aksu, G; Özyigit, G; Özkan, L; Kilçiksiz, S; Koca, S; Çaloglu, M; Yavuz, AA; Çaglar, HB; Beyzadeoglu, M; Igdem, S; Serin, M; Kaplan, B; Koç, M; Korkmaz, E; Karakoyun-Çelik, Ö; Dinçer, S; Kinay, M
    Purpose: To determine the patterns of care for lung cancer in Turkish radiation oncology centers. Methods and Materials: Questionnaire forms from 21 of 24 (87.5%) centers that responded were evaluated. Results: The most frequent histology was non-small cell lung cancer (NSCLC) (81%). The most common postoperative radiotherapy (RT) indications were close/(+) surgical margins (95%) and presence of pN2 disease (91%). The most common indications for postoperative chemotherapy (CHT) were >= IB disease (19%) and the presence of pN2 disease (19%). In Stage IIIA potentially resectable NSCLC, the most frequent treatment approach was neoadjuvant concomitant chemoradiotherapy (CHRT) (57%). In Stage IIIA unresectable and Stage IIIB disease, the most frequent approach was definitive concomitant CHRT (91%). In limited SCLC, the most common treatment approach was concomitant CHRT with cisplatin+etoposide for cycles 1-3, completion of CHT to cycles 4-6, and finally prophylactic cranial irradiation in patients with complete response (71%). Six cycles of cisplatin + etoposide CHT and palliative thoracic RT, when required, was the most commonly used treatment (81%) in extensive SCLC. Sixty-two percent of centers did not have endobronchial brachytherapy (EBB) facilities. Conclusion: There is great variation in diagnostic testing, treatment strategies, indications for postoperative RT and CHT, RT features, and EBB availability for LC cases. To establish standards, national guidelines should be prepared using a multidisciplinary approach. (c) 2008 Elsevier Inc.