Browsing by Author "Koyuncu, F"
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Item Increased vascular surface density in ovarian endometriosisInan, S; Kuscu, NK; Vatansever, S; Ozbilgin, K; Koyuncu, F; Sayhan, SOur goal in this study was to investigate the presence of atigiogenesis-related factors in endometriomas by evaluating their vascular surface densities. Thirty ovarian samples were included in the study. Of these, ten were histologically confirmed endometriomas, ten were ovarian specimens in the follicular phase and ten were ovarian specimens in the luteal phase, serving as controls. Histological specimens were immunostained for von Willebrand factor (vWF: factor VIII-related antigen) and CD34. The area with the highest microvessel density in endometriosis and in the normal ovary was evaluated by using an intercept grid. All microvessels in a specific field (X 100 magnification) were counted and vascular surface density was measured, as 164.01 +/- 21.26 vs. 125.15 +/- 11.28 and 117.44 +/- 9.27 by using vWF, and as 172.97 +/- 25.64 vs. 138.65 +/- 32.21 and 120.34 +/- 18.40 by using CD34 in endometriotic, follicular and luteal ovarian samples, respectively (p < 0.001). The mean vascular surface density was significantly higher in endometriosis than in the ovarian samples of the follicular phase or the luteal phase. No significant difference was seen between normal ovarian samples. Endometriosis was associated with angiogenic properties. Having demonstrated elevated angiogenic factors in endometriotic samples, we concluded that activation of angiogenesis might be a key factor in the pathogenesis of endometriosis.Item Can endometrial thickness measurements predict the results of histological dating?Yildirim, A; Hassa, H; Yaliin, O; Koyuncu, F; Atay, Y; Inan, MItem Immunolocalization of αV, α3 and β1 integrins in the human placenta with pre-eclampsiaVatansever, HS; Inan, VS; Lacin, S; Koyuncu, FSigns of pre-eclampsia are considered to be caused by maternal endothelial dysfunction due to circulating factors of placental origin. Integrins are a large family of cell surface proteins that serve as receptors involved in cell-cell and cell-matrix interactions during placentation. Therefore, low expression of integrins or the lack of it may be encountered during pre-eclampsia. In the present study, we investigated the immunolocalisation of integrins alphaV, alpha3 and beta1 in placentas of normal and preeclamptic women. Thirty-two placentas from pre-eclamptic (n = 14) and normotensive (n = 18) women were used. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissue specimens, using anti-alphaV, anti-alpha3 and anti-beta1 antibodies and the indirect immunoperoxidase technique. A semi-quantitative grading system (HSCORE) was used to compare immunohistochemical staining intensities. Distribution patterns of alphaV, alpha3 and beta1 integrins were detected in cytotrophoblasts and Hofbauer cells in normal and pre-eclamptic placentas. Immunostaining of alphaV and beta1 integrins was slightly decreased in pre-eclamptic samples but alpha3 integrin immunostaining was similar in pre-eclamptic and normal placentas. Decreased immunostaining of integrins in the cytotrophoblasts may considered to be a structural basis for decreased placental perfusion in pre-eclampsia.Item Immunohistochemical staining of IGF-I, IGF-binding proteins-1 and-3, and transforming growth factor beta-3 in the umbilical cords of preeclamptic patientsInan, S; Vatansever, S; Kuscu, NK; Laçin, S; Ozbilgin, K; Koyuncu, FBackground. To detect the immunoreactivity of insulin-like growth factor-I, insulin-like growth factor-binding proteins-1 and -3 and transforming growth factor beta-3 in the umbilical cords of normal and preeclamptic patients. Methods. Umbilical cords were obtained from 15 normal and 15 preeclamptic patients. Immunoreactivities were determined using either indirect immunofluorescence or immunoperoxidase techniques on formalin-fixed, paraffin-embedded sections. Staining intensity was graded by a semiquantitative scoring method. The results were compared by Mann-Whitney U -test. Results. The umbilical cords were thinner and the vessels were hypoplastic in the preeclamptic group. Moderate staining intensity for insulin-like growth factor-I, insulin-like growth factor binding protein-1 and -3 and transforming growth factor-beta 3 was observed in normal patients. The preeclamptic group had mild and strong intensities for insulin-like growth factor-I and insulin-like growth factor binding protein-1, respectively, and intensity for insulin-like growth factor binding protein-3 did not change, but diffuse and increased intensity was observed for transforming growth factor-beta 3. Conclusion. Changes in the intensity of insulin-like growth factor-I and its major binding protein and the transformation of growth factor-beta 3 may play a role in the pathogenesis of preeclampsia by altering the structure and responsiveness of the umbilical cordItem Effect of insulin on rat ovarian leptin expression by immunohistochemical stainingKuscu, NK; Ozbilgin, K; Inan, S; Giray, G; Ceylan, E; Koyuncu, FAim: Leptin and insulin may interact in regulating ovarian steroid synthesis. The objective of this study was to investigate immunohistochemical staining of leptin in normal rat ovarian tissues and in rats treated with insulin and insulin plus human chorinoic gonadotropin (hCG). Methods: Paraffin blocks of rat ovarian tissues from a previous study, in which 18 adult, female Wistar rats with an average weight of 250 g were divided into three groups to receive either saline solution, human insulin (2 U/day) or human insulin (2 U/day) plus hCG (4 U/day) for 4 weeks, were used in this study to compare the effects on leptin staining. The results were analysed using a semiquantitative scoring system, such as mild, moderate and strong. Results: No staining was observed in granulosa cells and theca interna cells of normal ovarian tissues. Theca externa cells had mild staining intensity (+), corpus luteum had moderate (+ +) and stroma had mild (+) staining intensity. Histological structure was impaired in the insulin group, luteinized cells had mild staining, there was no difference in other cell groups. Only theca externa cells of the developing follicles were stained in insulin plus hCG group, luteinized cells again had mild staining. Conclusions: Besides damaging the rat ovarian structure, insulin reduced staining intensity of leptin in luteinized cells. Insulin may stimulate ovarian steroid synthesis not only through its own receptors, but also by acting on the leptin expression of these cells.Item Rupture of rudimentary horn pregnancy at the 15th week of gestationKuscu, NK; Laçin, S; Kartal, Ö; Koyuncu, FA patient with acute abdomen at 15th week of gestation underwent an emergency laparatomy. A ruptured rudimentary horn pregnancy was diagnosed. and the rudimentary part including the fetus and the accessories was resected. The mother survived without any complications. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.Item Clomiphene citrate does not affect the secretion of α3, αV and β1 integrin molecules during the implantation window in patients with unexplained infertilityLacin, S; Vatansever, S; Kuscu, NK; Koyuncu, F; Ozbilgin, K; Ceylan, EBACKGROUND: The expression of integrin molecules on the endometrium suggests that certain integrins may participate in the cascade of molecular events leading to successful implantation. A prospective, controlled study was carried out to investigate the effect of clomiphene citrate (CC) on secretions of beta (1), alpha (3) and alpha (V) integrin molecules in the endometrium of patients with unexplained infertility during the implantation window. METHODS: A total of 40 endometrial samples was evaluated in both spontaneous (n = 13) and ensuing clomiphene-treated cycles (100 mg on days 5-9) and also from fertile women serving as controls (n = 14) during postovulatory 7th or 8th day of menstrual cycle. A semiquantitative grading system (H-score) was used to compare the inmumohistochemical staining intensities. Endometrial thickness and serum oestradiol and progesterone concentrations were also measured on the day of sampling. RESULTS: Staining of alpha (v) but not beta (1) and alpha (3) integrins was significantly less intense in infertile cases than fertile control cases (1.42 +/- 0.12 versus 2.21 +/- 0.13 respectively, P = 0.012) and this was not restored to normal concentrations with treatment. CONCLUSIONS: Our study indicated that cc treatment significantly decreased the endometrial thickness and increased oestradiol and progesterone concentrations. However, secretion of alpha (v,) beta (1) and alpha (3) integrin molecules, which might play a role in implantation, was not affected.Item Myxoid leiomyosarcoma of the uterusOruç, S; Koyuncu, F; Gürbüz, NItem The effects of hormone replacement therapy on serum leptin, IGF-1 and IGFBP-3 levels in postmenopausal womenUyanik, BS; Var, A; Koyuncu, F; Ari, Z; Onur, E; Yildirim, Y; Bülbül, YItem Hyperemesis gravidarum: current concepts and managementKuscu, NK; Koyuncu, FHyperemesis gravidarum is a common problem for an obstetrician. Though nausea and vomiting are quite common in pregnancy, hyperemesis is found in only 1-20 patients per 1000. In this practical review, a general outline of the syndrome, its relation to the gastrointestinal system and thyroid, mild and rare severe complications, and conventional treatment versus newer options are discussed.Item InsulinKuscu, NK; Koyuncu, F; Özbilgin, K; Inan, S; Tuglu, I; Karaer, ÖThe goal of this study was to investigate histological changes of the rat ovary treated with either insulin or insulin plus human chorionic gonadotropin (hCG). The study was conducted in Celal Bayar University, School of Medicine, Animal Research Laboratory. Eighteen adult female Wistar rats were divided into three groups to receive saline, or insulin, or insulin plus hCG for 4 weeks. At the end of treatment the rats were sacrificed and the ovaries were evaluated with hematoxylin and eosin. There was no abnormal change in. rats treated with saline. A thickened capsule, stromal hypertrophy and stromal cell hyperplasia, and no developing follicles, were observed in the insulin-only group. A thin capsule, developing follicles and corpora lutea, and normal theca cells and stroma were observed in the insulin-plus-hCG group. We conclude that insulin may lead to histological changes similar to stromal hyperthecosis and polycystic ovary syndrome, and may be one of the factors causing follicular arrest.Item Sexual behaviour during pregnancyOruc, S; Esen, A; Lacin, S; Adigüzel, H; Uyar, Y; Koyuncu, FThe effects of pregnancy on sexuality were studied in 158 pregnant women. They were surveyed by questionnaire about sociodemographic variables and sexual behaviour. Dyspareunia was common in our study group during pregnancy. Pregnancy had a negative effect on orgasmic quality. Dyspareunia and orgasmic quality influenced coital frequency. Coital frequency declined as the month of the pregnancy increased. Pregnancy is a potent influence on sexuality irrespective of an individual's conditioning.Item Immunolocalization of transforming growth factor-β3 in pregnant human myometrium -: Possible associations with laborKuscu, NK; Lacin, S; Vatansever, S; Yildirim, Y; Var, A; Uyanik, BS; Koyuncu, FBackground Transforming growth factor-beta3 is a cytokine which is involved in cell growth regulation and differentiation, stimulation of extracellular matrix and modulation of immune responses. The goal of this study was to detect the presence of this cytokine in the myometrium of preterm and term, nonlaboring and laboring patients, and to measure serum levels of interleukin-1beta (IL-1beta), IL-6 and IL-8 before cesarean section. Methods. In this prospective study, we obtained samples of myometrium from the lower uterine segment during elective and emergency cesarean sections (term non-laboring, n=8; term laboring, n=7; preterm non-laboring, n=3, and preterm laboring, n=19) and stained for transforming growth factor-beta3. Blood was also sampled from the same patients to determine IL-1beta, IL-6 and IL-8 levels. Results. Different intensities of staining were detected in preterm laboring, term nonlaboring and term laboring groups, but there was no staining in preterm nonlaboring group. We also found a statistically significant difference in IL-6 levels between laboring and nonlaboring groups (p=0.028). Conclusion. Different intensities of TGF-beta3 which appeared in different stages of myometrium made us consider that TGF-beta3 might prepare myometrium to labor, and IL-6 was more important than the other interleukins in initiation of labor.Item Collagenase treatment of sore nipplesKuscu, NK; Koyuncu, F; Laçin, SItem Assessment of the effectiveness of postmenopausal tibolone therapy on neural functions by measuring visual evoked potentialsLaçin, S; Oruç, S; Karaca, S; Kusçu, K; Koyuncu, F; Uyar, Y; Çaglar, HObjective: To assess the effect of hormone replacement therapy on neural transmission in postmenopausal women using tibolone by a non-invasive, objective way. Study design: In a randomised, 3 cycle, placebo-controlled study, neurovisual transmission in optic pathways were evaluated by measuring visual evoked potentials (VEP). After neuroophtalmologic examination, eligible subjects were randomised into two groups. Treatment group (n = 38) were given tibolone 2.5 mg daily continuously for 3 months and control group (n = 20) were treated with placebo. A baseline VEP measurement before the treatment and then at the end of first, second and third month were obtained by the EMG-evoked system in the Department of Neurology, University of Celal Bayar, Manisa, Turkey. Results: Data from 31 women from treatment group and 16 from control group were available for evaluation. The mean P(100) latency values, which indicate the transition time period between the optic stimuli and electrical change recorded on the occipital area by the skin electrodes, have showed a significant decrease for the study group (from 100.39 +/- 0.58 to 97.90 +/- 0.65 ms, P < 0.01) at the end of the first month of treatment according to the baseline values. This difference between two groups has remained constant during the study period. Conclusion: The change in latency measurements of VEP, reflecting the functional status in optic pathways from retina to occipital cortex were significantly different in the treatment group than in that of control. We concluded that a facilitating effect of tibolone was observed on neurovisual transmission. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.Item Mid-luteal phase salivary progesterone concentrations in ovulation induction cyclesHassa, H; Yildirim, A; Atay, Y; Koyuncu, F; Inan, M