Browsing by Author "Kum, S"

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    The Distribution and Heterogeneity of Mast Cells in the Cecum of Quail (Coturnix Coturnix Japonica)
    Yildiz, M; Aydemir, I; Kum, S; Eren, Ü
    The aim of the present study is to investigate the location and heterogeneity of mast cells in quail cecum. Cecum samples were fixed in basic lead acetate (BLA), Carnoy's, isotonic formaldehyde acetic acid (IFAA) and 10% neutral buffered formalin (NBF) solutions. The sections were stained with an alcian blue-critical electrolyte concentration (AB-CEC) (pH 5.8, 0.3 M MgCl2) and safranin O (SO) (pH 1.0) used in a combined method and with toluidine blue (TB) (pH 0.5). Mast-cell population was shown in highest ratios in all layers of Carnoy fixed cecum parts within TB-stained sections. Metachromatic mast cell density was determined the most in tunica mucosa layers of the middle and distal cecum. It was seen that AB-CEC (+)/SO (-) mast-cell density was greater in tunica mucosa layers of the proximal and middle cecum in IFAA and BLA fixed tissues campare to Carnoy fixation. At the end of the study; it can be said that Carnoy solution fixed the connective tissue mast cells, as well as IFAA and BLA solutions fixed the mucosal mast cells better.
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    Histological investigations on thymus of male rats prenatally exposed to bisphenol A
    Aydemir, I; Kum, S; Tuglu, MI
    Bisphenol A is called as a endocrine-distrupting chemical because of the its steroid-like activity and it used in the construction of plastic containing materials. It is indicated that bisphenol A can pass the human serum, urine, follicular fluid, placenta and umblical cord as a result of the use of substances containing this agent. In this study, we aimed to investigate the effects of bisphenol A on the development of the thymus, a primary lymphoid organ which plays an important role in the specific immunity. The adult pregnant female rats were administered orally with bisphenol A (for 21 days) and postnatal thymus samples were obtained on day 21, 45 and 90 and were performed for histochemical and immunohistochemical staining for CD3, CD4, CD8 and CD79a and TUNEL assay for the apoptotic cells. Evaluation of all groups, CD3, CD4, CD8 and CD79a stainings were decreased in the experimental groups compared with control group. The apoptotic cells were determined in the all groups on day 90 as a result of the thymus involution. It is noted that there was not any histological and morphological damages in the rats prenatally exposed the bisphenol A. The effect of the bisphenol A is unknown in the future, but there is no problem in the adult rats. (C) 2018 Elsevier Ltd. All rights reserved.
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    Investigation of the effects of bisphenol-A exposure on lymphoid system in prenatal stage
    Aydemir, I; Özbey, C; Özkan, O; Tuglu, MI; Kum, S
    Bisphenol-A (BPA) used in the production of plastic materials is a temperature-soluble agent. It also has a steroid hormone-like activity; therefore, it poses a danger to human health. In our study, we aimed to investigate the effects of BPA on lymph node and spleen in male rats exposed to this agent during prenatal stage. The pregnant female rats were divided into four groups: control, sham, low dose (300 mu g/kg BPA), and high dose (900 mu g/kg BPA). BPA was dissolved in 1 mL of corn oil and administered to the pregnant rats every day during pregnancy. On the 21st and 45th day after the birth, male rats' lymph node and spleen samples were taken and histopathological examination was performed. Samples were stained with hematoxylin and eosin to determine the general histological appearance, and with CD3 and CD20 immunohistochemically. The results of staining were evaluated by H-score, and statistical analysis was performed. In the samples, BPA applications were not found to cause significant tissue damage. But there was a significant decrease in the immunoreactivities of CD3 and CD20 after BPA applications in both 21st and 45th day samples. After high dose BPA administration, decreased CD3 immunoreactivity was statistically significant. It is thought that BPA does not cause histologically significant tissue damage, but it may impair organ function at cellular level. The investigation of molecules involved in organ function will be useful in revealing the mechanisms that will cause dysfunction.