Browsing by Author "Kurt, FÖ"

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    A Cytogenetic Study on the Some Western Anatolian Triturus Species (Salamandridae, Urodela)
    Kurt, FÖ; Koca, S
    The karyotypes of two species in the name of Triturus vulgaris and Triturus karelinii, belonging Salamandridae family of Urodela order were analyzed. Chromosome numbers of both the Triturus species were identified as 2n=24 and chromosomes forming karyotypes clustred in three group, long, medium and short by their lengths. I, VIII and XIIth chromosome pairs in Triturus vulgaris showed metacentric morphology whereas the same chromosomes showed submetasentric morphology in Triturus karelinii. VI, VII, IX, X and XIth chromosome pairs in Triturus vulgaris were found to be submetasentric compared to metasentric morphology of same chromosome pairs in Triturus karelinii. The other chromosome pairs II, III, IV and V-th were found to have metasentric morphology in both species. According to our measurements, chromosomes of Triturus karelinii were found to be long and to have more regular line and intrakaryotypic variations between chromosomes were determined as low.
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    The effects of tramadol on cancer stem cells and metabolic changes in colon carcinoma cells lines
    Özgürbüz, U; Gencür, S; Kurt, FÖ; Özkalkanli, M; Vatansever, HS
    Opioids are widely used in the treatment of cancer related pain. They mainly exert their effects on opioid receptors. The most common opioid in the treatment of pain is morphine. Previous studies show that they may have effects on cancer cell behavior. These may include apoptosis, angiogenesis, invasion, inflammation and immune reactions. Tramadol, also an opioid is widely used in the treatment of cancer pain and is not well studied in cancer behavior. We aimed to investigate the effects of tramadol on cancer stem cells and metabolic changes in colon carcinoma cells. We used Co1o320 (ATCC, CCL-220), Colo741 (ECACC, 93052621) and HCT116 (ATCC, CCL-247) colon cancer cell lines. CD133 was considered colon cancer stem cell marker and used to sort CD133+ and CD133- cells by magnetic cell sorting. Dm (mitochondria-targeted therapeutics) technique was used to detect tramadol's cytotoxic effect on cells in the study groups. Cells were treated with 1 mg/kg, 1.5 mg/kg and 2 mg/kg tramadol for 24 h at 37 degrees C and 5% CO2.Caspase-3, Ki-67, Bcl-2 and VGEF distributions were performed using indirect immunoperoxidase staining for immunohistochemical analysis. The study showed that tramadol has triggering effect on apoptosis in Colo320 colon cancer stem cells.
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    Random/aligned electrospun PCL/PCL-collagen nanofibrous membranes: comparison of neural differentiation of rat AdMSCs and BMSCs
    Çapkin, M; Çakmak, S; Kurt, FÖ; Gümüsderelioglu, M; Sen, BH; Türk, BT; Deliloglu-Gürhan, SI
    In this study, the aligned (A) and randomly oriented (R) polycaprolactone (PCL-A and PCL-R) and PCL/collagen (PCL/Col-A and PCL/Col-R) nanofibers were electrospun onto smooth PCL membranes (PCLMs) prepared by solvent casting. In order to investigate the effects of chemical composition and nanotopography of fibrous surfaces on proliferation and on neural differentiation of mesenchymal stem cells (MSCs), adipose and bone marrow-derived rat MSCs (AdMSCs and BMSCs) were cultivated in suitable media i.e. inducing medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF), and cell maintenance medium (CMM). BMSCs adhered and proliferated on all nanofibrous membranes more efficiently than AdMSCs. PCL/Col-A was found as the most convenient surface supporting proliferation in both cell types. Immunofluorescence staining indicated that BMSCs and AdMSCs are prone for differentiation to oligodendrocytes more than they differentiate to other neuronal cell types. PCL-A nanofibrous membranes supported differentiation of MSCs to O4(+) (an oligodendrocytes surface antigen) cells in both culture media. The intensity of immunoreactivity of O4(+) cells differentiated from BMSCs on PCL-A was highest when compared with the other groups (p < 0.001). Some BIII-T signed neural cells were investigated on PCL-A nanofibrous membranes, but the intensity of immunoreactivity was lower than that of O4(+) cells. In conclusion, this study can be evaluated to establish the cell therapy strategies in neurodegenerative disorders, which are relevant to oligodendrocyte abstinence using BMSCs or AdMSCs on aligned nanofibrous membranes.
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    Proapoptotic and Anticancer Potentials of Thymus capitatus Essential Oil on Colon Cancer Stem Cells
    Yavuz, DÖ; Becer, E; Kendirci, R; Kurt, FÖ; Vatansever, HS
    BACKGROUND/AIMS The aim of this study was to investigate the proapoptotic activity of Thymus capitatus essential oil in either colon cancer stem (CDI33+ Colo-320) or nonstem (CDI33- Colo-320) cells. MATERIAL and METHODS T. capitatus essential oil was obtained by water distillation and analyzed by GC-MS. Cancer stem cells (CDI33+ Colo-320) were obtained from the Colo-320 cells by the MiniMACS system. Proapoptotic activity of T capitatus essential oil was investigated by immunocytochemistry using antibodies directed against caspase-3 and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. RESULTS Caspase-3 immunoreactivity was significantly increased in 0.5% dilution T. capitatus essential oil-treated Colo-320 cells for 48 hours Moreover, the number of TUNEL positive cells was significantly higher in Colo-320 cells when compared with CDI33+ and CDI33- Colo320 cells. CONCLUSION We conclude that T capitatus essential oil increases caspase-3 molecules, which play a crucial role in apoptosis. Interestingly, T capita- tus essential oil is found to be more effective in Colo-320 cells than CDI33+ Colo-320 and CDI33- Colo-320 cells in terms of apoptosis.