Browsing by Author "Kurt F.O."

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    Potential clinical use of differentiated cells from embryonic or mesencyhmal stem cells in orthopaedic problems
    (Bentham Science Publishers, 2016) Kurt F.O.; Vatansever H.S.
    Stem cells are classified by their tissue source. Embryonic stem cells that are derived from the inner cell mass of blastocyst stage embryos are highly proliferative in their undifferentiated state. A multipotent type of mesenchymal stem cells is isolated from various types of tissues such as bone marrow, fat tissue etc. The dynamics of embryonic and adult stem cell cycles are profoundly dissimilar from the culture of stem cells. After improving the culture conditions and differentiation potentials, differentiated stem cells are the first cells to be preferred in modern regenerative medicine and tissue engineering. This review article focuses on the cell-based therapy of orthopedic problems. We explore the challenges associated with bone repair and regeneration using embryonic or mesenchymal stem cells that are in undifferentiated or/and differentiated condition. This paper also discusses optimizing the best cell type, differentiation condition and using them on bone tissue engineering for future investigations. © 2016 Bentham Science Publishers.
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    Effects of caffeic acid phenethyl ester on matrix molecules and angiogenetic and anti-angiogenetic factors in gastric cancer cells cultured on different substrates
    (Taylor and Francis Ltd, 2016) Kosova F.; Kurt F.O.; Olmez E.; Tuʇlu I.; Ar Z.
    Migration, invasion, metastasis and angiogenesis associated with cancer depend on the surrounding microenvironment. Angiogenesis, the growth of new capillaries, is a regulator of cancer growth and a useful target for cancer therapy. We examined matrix protein interactions in a gastric cancer cell culture that was treated with different doses of caffeic acid (3,4-dihydroxycinnamic acid) phenethyl ester (CAPE). We also investigated the relations among the levels of vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), endostatin (ES) and trombospondin-1 (TSP-1). Cytotoxity of CAPE was measured using the 3-(4,5-dmethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. We examined the behavior of cells on laminin and collagen I coated surfaces in response to the angiogenic effect of these matrix molecules. We examined the protein alterations of these matrix molecules immunohistochemically and measured the levels of VEGF, MMP-9, ES and TSP-1 using the ELISA test. We showed that application of CAPE to the gastric cancer cell line on tissue culture plastic, laminin and collagen I significantly decreased the VEGF and MMP-9 protein levels. We found that TSP-1 levels were increased significantly in the gastric cancer cells after application of CAPE. The protein levels of gastric cancer cells also were increased significantly when tissue was cultured on laminin and collagen I. Application of CAPE to cells on laminin or collagen I coated surfaces significantly increased all of the proteins except ES. ES levels were increased on the collagen I covered surfaces, but the laminin surface decreased the levels of ES significantly. We demonstrated the beneficial effect of CAPE on a gastric cancer cell line including inhibition of proliferation and induction of some proteins that might be related to decreased angiogenesis. © 2015 The Biological Stain Commission.
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    Role of Bone Marrow-Derived Stem Cells in Wound Healing
    (Wiley Blackwell, 2018) Kurt F.O.; Vatansever H.S.; Uluer E.T.
    Wound healing is a complex process and some conditions such as diabetes is difficult to treat. In these conditions external cell support is required where mesenchymal stem cells can be functional and useful. One of the main sources for these cells is bone marrow. These cells can be separated from hematopoietic precursors by adhesive properties where they can proliferate, differentiate, and expand for clinical use. Their effect on wound healing depends on their interaction with other cells, in which their production of cytokine and growth factors play an important role during the healing process, especially for immune modulation. In this review, the effect of stem cells on wound healing with cell interaction, regulation of the matrix, and adhesion with secretion of cells will be discussed in order to understand the mechanisms used by these cells to achieve a better quality of life for the patient suffering from a non-healing wound. © 2018 John Wiley & Sons, Inc. All rights reserved.
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    Wound Healing and Microenvironment
    (Wiley Blackwell, 2018) Uluer E.T.; Vatansever H.S.; Kurt F.O.
    The wound healing process includes activation of signaling pathways that work together to restore a tissue microenvironment consisting of cells and extracellular matrix (ECM) with enzymes, cytokines, and growth factors. Wound healing consists of a dynamic process including various overlapping stages that include hemostasis and inflammation, cell proliferation, and maturation together with remodeling. In this chapter, we focus on wound healing types and the importance of the microenvironment. We then discuss the mechanism of wound healing and the interactions between molecules, cells, and extracellular matrix in the microenvironment during wound healing. © 2018 John Wiley & Sons, Inc. All rights reserved.
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    Synthesis and cytotoxic activities of organometallic Ru(II) diamine complexes
    (Academic Press Inc., 2020) Kavukcu S.B.; Şahin O.; Seda Vatansever H.; Kurt F.O.; Korkmaz M.; Kendirci R.; Pelit L.; Türkmen H.
    A series of mono and bimetallic ruthenium(II) arene complexes bearing diamine (Ru1-6) were prepared and fully characterized by 1H, 13C, 19F, and 31P NMR spectroscopy and elemental analysis. The crystal structure of the bimetallic complex (Ru5) was determined by X-ray crystallography. Monometallic analogues (Ru1-3) were synthesized to investigate the contributions of ruthenium and the other organic groups (aren, ethylenediamine, butyl) to the activity. The electrochemical behaviors of mono and bimetallic complexes were obtained from the relationship between cyclic voltammetry (CV) and the biological activities of the compounds. The cytotoxic activities of the complexes (Ru1-6) were tested against wide-scale cancer cell lines, namely HeLa, MDA-MB-231, DU-145, LNCaP, Hep-G2, Saos-2, PC-3, and MCF-7, and normal cell lines 3T3-L1 and Vero. Diamine Ru(II) arene complexes have unique biological characteristics and they are promising models for new anticancer drug development. MTT analysis reveals that each synthesized Ru complex showed cytotoxic activity towards the different cancer cells. In particular, three Ru complexes (Ru3, Ru5 and Ru6) showed less toxic effects on the cancer cells than the others. These novel Ru complexes affected both cancer and normal cell lines. As they had a toxic effect on the cells, the dosage applied should be tested before being used for in vivo applications. Cytotoxicity tests have shown that the bimetallic complex Ru6 was effective on all cancer cells. The effect of bimetallic enhancement on cancer cell lines, the systematic variation of the intermetallic distance and the ligand donor properties of the mono and bimetallic complexes were explored based on the cytotoxic activity. The interaction with FS-DNA and the stability/aquation of the complexes (Ru3 and Ru6) were investigated with 1H NMR spectroscopy. The binding modes between the complexes (Ru3 and Ru6) and DNA were investigated via UV–Vis spectroscopy. © 2020 Elsevier Inc.
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    Effect of geldanamycin on the expression of the matrix molecules and angiogenetic factors in a gastric cancer cell line
    (Taylor and Francis Ltd., 2021) Gürpınar T.; Kosova F.; Kurt F.O.; Cambaz S.U.; Yücel A.T.; Umur N.; Tuğlu M.I.
    Angiogenesis is the formation of new blood vessels. Angiogenesis affects cancer growth and is a useful target for cancer therapeutics. The effects of geldanamycin on angiogenesis in cases of gastric cancer are poorly understood. We investigated the effects of different doses of 17-allylamino-17-demethoxygeldanamycin (17-AGG), a semi-synthetic derivative of geldanamycin, on the interactions between cellular matrix proteins and angiogenesis factors in a gastric cancer cell line. We examined cancer cells on laminin and collagen I coated surfaces to determine their response to the angiogenic effect of these matrix molecules. We also evaluated the expression levels of VEGF, MMP-9, ES and TSP-1 using ELISA. We found that application of 17-AAG to the gastric cancer cell line on culture dish plastic decreased VEGF, TSP-1, ES and MMP-9 expression, whereas of all of these proteins were increased by laminin and collagen coating. 17-AAG currently is in clinical trial phase 2 and may be a promising drug for treatment of gastric cancer. © 2020 The Biological Stain Commission.