Browsing by Author "Lacin, S"

Now showing 1 - 12 of 12
  • No Thumbnail Available
    Item
    Changed Bcl:Bax ratio in endometrium of patients with unexplained infertility
    Vatansever, HS; Lacin, S; Ozbilgin, MK
    Apoptosis has been shown to be an important regulator of endometrial function during the menstrual cycle and implantation. Recently, some possible implantation defects were identified in patients with unexplained infertility. In this study, we investigated the role of spontaneous apoptosis, which is regulated by death regulatory genes, such as Bc1-2, Bax, p53, and isoenzymes of nitric oxide synthases; eNOS and iNOS during the implantation window in women with unexplained infertility. Endometrial samples were evaluated from fertile (n = 15) and unexplained-infertile women (n = 15) during post-ovulatory 7th or 8th day of their menstrual, cycles. Apoptotic cells were detected using the dUTP nick-end labelling assay and Bcl-2, Bax, p53, iNOS and eNOS were assessed immunohistochemically. Reduced apoptotic cells, weak immunoreactivity of p53 and strong immunoreactivity of Bcl-2 were observed in the unexplained-infertile group compared with the fertile group (p < 0.001). Bax intensity was similar in both groups. White weak iNOS immunoreactivity was detected in both groups, moderately increased eNOS immunoreactivity was observed in infertile cases. Spontaneous apoptosis is reduced in the endometrium of unexplained-infertile women, and is associated with the changed Bcl-2:Bax ratio. This finding may be a contributing factor to defective implantation causing infertility in this group of patients. (c) 2005 Elsevier GmbH. All rights reserved.
  • No Thumbnail Available
    Item
    Immunolocalization of αV, α3 and β1 integrins in the human placenta with pre-eclampsia
    Vatansever, HS; Inan, VS; Lacin, S; Koyuncu, F
    Signs of pre-eclampsia are considered to be caused by maternal endothelial dysfunction due to circulating factors of placental origin. Integrins are a large family of cell surface proteins that serve as receptors involved in cell-cell and cell-matrix interactions during placentation. Therefore, low expression of integrins or the lack of it may be encountered during pre-eclampsia. In the present study, we investigated the immunolocalisation of integrins alphaV, alpha3 and beta1 in placentas of normal and preeclamptic women. Thirty-two placentas from pre-eclamptic (n = 14) and normotensive (n = 18) women were used. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissue specimens, using anti-alphaV, anti-alpha3 and anti-beta1 antibodies and the indirect immunoperoxidase technique. A semi-quantitative grading system (HSCORE) was used to compare immunohistochemical staining intensities. Distribution patterns of alphaV, alpha3 and beta1 integrins were detected in cytotrophoblasts and Hofbauer cells in normal and pre-eclamptic placentas. Immunostaining of alphaV and beta1 integrins was slightly decreased in pre-eclamptic samples but alpha3 integrin immunostaining was similar in pre-eclamptic and normal placentas. Decreased immunostaining of integrins in the cytotrophoblasts may considered to be a structural basis for decreased placental perfusion in pre-eclampsia.
  • No Thumbnail Available
    Item
    Assessment of fetal cerebral arterial and venous blood flow before and after vaginal delivery or Cesarean section
    Baytur, YB; Tarhan, S; Uyar, Y; Ozcakir, HT; Lacin, S; Coban, B; Inceboz, U; Caglar, H
    Objectives To compare perinatal intracranial arterial and venous blood velocity changes between healthy term neonates delivered vaginally or by Cesarean section and to correlate these changes with cord blood gases and pH values at birth. Methods The study involved 43 healthy term neonates who were delivered vaginally (n = 20) or by Cesarean section (n = 23). All fetuses/neonates were examined by Doppler ultrasound to obtain middle cerebral artery (MCA) and cerebral transverse sinus (Tsin) Doppler waveforms on three occasions (before delivery, and 1 h and 24 h after birth). Pulsatility index (PI) and peak systolic velocity (PSV) for MCA and Tsin were measured and compared between neonates who were delivered vaginally or by Cesarean section. Umbilical cord blood samples were analyzed for umbilical artery and vein pH, pO(2) and pCO(2) and values were correlated with MCA and Tsin Doppler indices. Results MCA-PI increased and MCA-PSV decreased at 1 h after birth, and Doppler measurements returned to predelivery values at 24 h after birth. Tsin Doppler measurements remained unchanged at 1 h and 24 h when compared to predelivery values in both the Cesarean and vaginal delivery groups. There was a negative correlation between Tsin-PI before birth and umbilical venous pH. There was a positive correlation between Tsin-PSV at 1 h after birth and umbilical vein PCO2. Conclusions Cerebral arterial blood velocity decreases immediately after birth and increases within 24 h, probably as part of neonatal adaptation. Cerebral venous blood velocity remains constant during the perinatal period and is likely to be regulated in a different and more complex manner than that of arterial blood velocity. Mode of delivery does not affect cerebral blood velocity. Copyright (C) 2004 ISUOG. Published by John Wiley Sons, Ltd.
  • No Thumbnail Available
    Item
    Clomiphene citrate does not affect the secretion of α3, αV and β1 integrin molecules during the implantation window in patients with unexplained infertility
    Lacin, S; Vatansever, S; Kuscu, NK; Koyuncu, F; Ozbilgin, K; Ceylan, E
    BACKGROUND: The expression of integrin molecules on the endometrium suggests that certain integrins may participate in the cascade of molecular events leading to successful implantation. A prospective, controlled study was carried out to investigate the effect of clomiphene citrate (CC) on secretions of beta (1), alpha (3) and alpha (V) integrin molecules in the endometrium of patients with unexplained infertility during the implantation window. METHODS: A total of 40 endometrial samples was evaluated in both spontaneous (n = 13) and ensuing clomiphene-treated cycles (100 mg on days 5-9) and also from fertile women serving as controls (n = 14) during postovulatory 7th or 8th day of menstrual cycle. A semiquantitative grading system (H-score) was used to compare the inmumohistochemical staining intensities. Endometrial thickness and serum oestradiol and progesterone concentrations were also measured on the day of sampling. RESULTS: Staining of alpha (v) but not beta (1) and alpha (3) integrins was significantly less intense in infertile cases than fertile control cases (1.42 +/- 0.12 versus 2.21 +/- 0.13 respectively, P = 0.012) and this was not restored to normal concentrations with treatment. CONCLUSIONS: Our study indicated that cc treatment significantly decreased the endometrial thickness and increased oestradiol and progesterone concentrations. However, secretion of alpha (v,) beta (1) and alpha (3) integrin molecules, which might play a role in implantation, was not affected.
  • No Thumbnail Available
    Item
    Pattern reversal visual evoked potentials in different reproductive stages of females
    Yilmaz, H; Mavioglu, H; Erkin, EF; Oksel, F; Lacin, S
    Estrogens increase after puberty and remain at high levels in the reproductive period and decrease with menopause. These changes occuring in the level of estrogens affect dopamine metabolism indirectly by causing changes in cathecholamine sensitivity of the brain. To assess the role of estrogens on the formation of pattern reversal evoked potentials (PRVEPs), three groups of patients were considered: prepubertal group, reproductive age group and postmenopausal group. PRVEPs of all subjects were recorded. Mean latency was shortest in the reproductive age. While no statistical difference was present between the mean latencies of prepubertal and the reproductive period groups, the mean latencies of both of these groups were statistically significantly shorter than that of the postmenopausal group(p<0.005).
  • No Thumbnail Available
    Item
    Sexual behaviour during pregnancy
    Oruc, S; Esen, A; Lacin, S; Adigüzel, H; Uyar, Y; Koyuncu, F
    The effects of pregnancy on sexuality were studied in 158 pregnant women. They were surveyed by questionnaire about sociodemographic variables and sexual behaviour. Dyspareunia was common in our study group during pregnancy. Pregnancy had a negative effect on orgasmic quality. Dyspareunia and orgasmic quality influenced coital frequency. Coital frequency declined as the month of the pregnancy increased. Pregnancy is a potent influence on sexuality irrespective of an individual's conditioning.
  • No Thumbnail Available
    Item
    Immunolocalization of transforming growth factor-β3 in pregnant human myometrium -: Possible associations with labor
    Kuscu, NK; Lacin, S; Vatansever, S; Yildirim, Y; Var, A; Uyanik, BS; Koyuncu, F
    Background Transforming growth factor-beta3 is a cytokine which is involved in cell growth regulation and differentiation, stimulation of extracellular matrix and modulation of immune responses. The goal of this study was to detect the presence of this cytokine in the myometrium of preterm and term, nonlaboring and laboring patients, and to measure serum levels of interleukin-1beta (IL-1beta), IL-6 and IL-8 before cesarean section. Methods. In this prospective study, we obtained samples of myometrium from the lower uterine segment during elective and emergency cesarean sections (term non-laboring, n=8; term laboring, n=7; preterm non-laboring, n=3, and preterm laboring, n=19) and stained for transforming growth factor-beta3. Blood was also sampled from the same patients to determine IL-1beta, IL-6 and IL-8 levels. Results. Different intensities of staining were detected in preterm laboring, term nonlaboring and term laboring groups, but there was no staining in preterm nonlaboring group. We also found a statistically significant difference in IL-6 levels between laboring and nonlaboring groups (p=0.028). Conclusion. Different intensities of TGF-beta3 which appeared in different stages of myometrium made us consider that TGF-beta3 might prepare myometrium to labor, and IL-6 was more important than the other interleukins in initiation of labor.
  • No Thumbnail Available
    Item
    Non-invasive evaluation of the adaptations of cardiac function in the neonatal period
    Coskun, S; Yüksel, H; Bilgi, Y; Lacin, S; Tansug, N; Onag, A
    Postnatal adaptations of cardiac hemodynamics in infants born vaginally or by caesarean section may be different. These cardiac functions were evaluated by Doppler echocardiography to assess adaptation differences. Cardiac output, heart rate, stroke volume, mean arterial pressure, total systemic vascular resistance, ejection fraction, and ductus arteriosus diameter were determined and compared at 1, 24 and 72 h of life in 22 infants born vaginally (group 1) and 23 born by caesarean section (group 2). One hour after delivery, heart rate, mean blood pressure, and total systemic resistance were found to be higher in group 1 infants (P < 0.01, P < 0.05, P < 0.05 respectively). Stroke-volume measurements were significantly higher in group 2 (P < 0.05). The ejection fraction and cardiac output values were similar in both groups. At 24 and 72 h, no significant differences were observed in measurements of infants born vaginally or by caesarean section. We did not find a parameter negatively affecting healthy newborns in either mode of delivery. However, under pathological conditions affecting the cardiovascular system at 1 h of life, including perinatal infections and hypoxemia, a lower stroke volume, higher heart rate, higher mean blood pressure, and higher peripheral resistance may cause additional work load to the cardiovascular system in infants born vaginally.
  • No Thumbnail Available
    Item
    A comparative study of the effect of raloxifene and gosereline on uterine leiomyoma volume changes and estrogen receptor, progesterone receptor, bcl-2 and p53 expression immunohistochemically in premenopausal women
    Baytur, YB; Ozbilgin, K; Cilaker, S; Lacin, S; Kurtul, O; Oruc, S; Koyuncu, FM
    Objective: To compare the mechanism of action of raloxifene and gosereline induced shrinkage of leiomyomas via estrogen receptor, progesterone receptor, bcl-2 and p53 expression immunohistochemically. Study design: Thirty-two premenopausal women affected by uterine leiomyomas were randomized into two equal groups. Group A was treated with gosereline (3.6 mg subcutaneous injection monthly) and group B was treated with raloxifene (60 mg daily per os) for 3 months before undergoing surgery. At entry and at the end of the treatment the leiomyoma volume was measured ultrasonografically and the volume change was calculated. Immunohistochemical detection of estrogen receptor (ER), progesterone receptor (PR), bcl-2 and p53 were performed on leiomyoma tissue samples from group A, group B and the matched-control group. H-scores for ER, PR, bcl-2 and p53 were calculated. The mean volume changes of leiomyomas and immunohistochemical H-score differences of ER, PR, bcl-2 and p53 were compared between groups. Results: The leiomyoma volume decreased significantly after treatment in gosereline group from baseline of 65 cm(3) to 35 cm(3), and in raloxifene group from 68 cm(3) to 50 cm(3), p < 0.05. The difference between the before and after treatment leiomyoma volumes between the two treatments was not statistically significant. H-score of ER expression was significantly lower in gosereline group compared to control group (54.4 versus 113.2, p = 0.001), whereas H-score of PR expression was significantly lower with both gosereline and raloxifene groups compared to control group (64.8 for gosereline versus 94.6 for control, 73.6 for raloxifene versus 94.6 for control, p = 0.001). The bcl-2 expression was higher in both gosereline and raloxifene groups compared to control group (173.7 for gosereline versus 94.7 for control, 179.7 for raloxifene versus 94.7 for control, p = 0.001). The p53 expression was only lower with gosereline than the control group (169.4 versus 205.6, p = 0.001), whereas there was no significant change between the raloxifene group and the control group (201.9 versus 205.6) (p > 0.05). Conclusion: Raloxifene was as effective as gosereline in reducing leiomyoma volumes. Decreased PR expression may be a mechanism for tumor growth reduction in raloxifene treatment. In both treatment modalities, the mechanism of shrinkage of leiomyomas could not be increased apoptosis mediated by bcl-2 and p53 expression and should be investigated by further studies. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
  • No Thumbnail Available
    Item
    Immunohistochemical detection of transforming growth factor-α, epidermal growth factor, and vascular endothelial growth factor expression in hyperstimulated rat ovary
    Ozcakir, HT; Giray, SG; Ozbilgin, MK; Uyar, Y; Lacin, S; Caglar, H
    Objective. The aim of the present study is to figure out the immunohistochemical expression of transforming growth factor-alpha (TGF-alpha), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF) in hyperstimulated rat ovaries. Methods. Twenty Wistar-Albino adult female rats (250-300 g) were taken into the study. The animals were randomly divided into two groups, each containing 10 rats: (i) stimulation group and (ii) control group. In the stimulation group, a stimulation regimen was administered to induce follicular maturity and ovarian hyperstimulation syndrome (OHSS) at the end using a 30-IU follicle-stimulating hormone that was administered subcutaneously for 4 consecutive days, followed by a 30-IU human chorionic gonadotropin on day 5 to induce ovulation. The rats, in the control group, received 0.2 ml of 0.9% NaCl for 5 consecutive days to mimic the conditions of the study animals. At the end of the treatment period, all rats underwent ovariectomy and the sections of ovaries were stained for the TGF-alpha, EGF, and VEGF. Results. The expression of TGF-alpha, EGF, and VEGF in the endothelium, the stroma, the granulosa cells, and the corpus luteum was found to be significantly higher in the stimulated group, compared to that in the control group ( p < 0.05). Conclusion. TGF-alpha, EGF, and VEGF are found to have increased in the hyperstimulated ovaries and this finding seems to be involved in the OHSS pathogenesis.
  • No Thumbnail Available
    Item
    Weil's syndrome in pregnancy
    Baytur, YB; Lacin, S; Koyuncu, FM; Cabuk, M; Ceylan, C; Kandiloglu, AR
  • No Thumbnail Available
    Item
    Real-world efficacy and safety data of immune checkpoint inhibitors in Turkish patients with metastatic melanoma: A Turkish oncology group study
    Ozgun, MA; Dogan, I; Eryilmaz, MK; Erdogan, AP; Ayhan, M; Hafizoglu, E; Tolunay, PK; Cavdar, E; Cevik, GT; Demir, H; Dulgar, O; Yilmaz, B; Cakir, E; Gokyer, A; Unal, OU; Perkin, P; Sakalar, T; Gulmez, A; Tasci, ES; Lacin, S