Browsing by Author "Mandiracioglu A."

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    Investigation of the prevalence of amoebiasis in Izmir province and determination of Entamoeba spp. using PCR and enzyme immunoassay
    (2008) Kurt O.; Demirel M.; Ostan I.; Sevil N.R.; Mandiracioglu A.; Tanyuksel M.; Ak M.; Dagci H.
    Amoebiasis is a common and life-threatening disease. The discrimination of the pathogenic Entamoeba histolytica from the non-pathogenic Entamoeba dispar could be done by advanced methods such as enzyme immunoassay (EIA) and PCR. The aim of this study was to investigate the prevalence of amoebiasis in Izmir province, and differentiate the Entamoeba species by PCR and EIA. Stool samples of 2,047 individuals were examined by direct microscopy, formalin ethyl acetate concentration, trichrome staining and culture, and those found to be positive for E. histolytica/dispar by any of these methods were further analyzed by PCR and EIA for species identification. Fifty-nine of 2,047 (2.9%) stool samples were found to be positive for E. histolytica/dispar with microscopy and/or culture. Among these positive samples, E. histolytica was detected in 14 (23.7%) and 5 (8.5%) samples with PCR and antigen-specific ELISA (EIA), respectively. E. dispar was diagnosed in 31 (52.5%) and 52 (88.1%) of 59 samples with species-specific PCR and EIA, respectively. Risk factors related to infection with Entamoeba spp. and other intestinal parasites included living in shanty houses (p<0.01), a history of recent immigration to Izmir (p<0.01), having no social security (p<0.05) and living with a crowded family (p<0.01). The results demonstrated the significance of amoebiasis as a public health problem among people with low socio-economic status in Izmir province.
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    The prevalence of intestinal parasites in the province of Izmir, Turkey
    (2008) Dagci H.; Kurt Ö.; Demirel M.; Östan I.; Azizi N.R.; Mandiracioglu A.; Yurdagül C.; Tanyüksel M.; Eroglu E.; Ak M.
    Stool samples of a total of 2,047 people in Izmir province were examined by wet mount, formalin ethyl acetate concentration, and trichrome staining methods with an aim to reveal the prevalence of intestinal parasites in Izmir together with related personal and environmental risk factors. Geographical mapping showing the density and variation of the species of intestinal parasites in Izmir was done after all maps were scaled, and the coordinates were determined with GeoMedia5.0®. The prevalence of the intestinal parasites was found to be 25.6 % in Izmir, with a variation between the districts. Blastocystis hominis was the leading parasite, and the prevalence of parasites was higher in children compared to adults; however, the difference was statistically insignificant. There was also no significant difference between the parasite prevalence and sex, marital status, education, income, frequent eating outside, and habitual raw meat eating. Significant differences were found between the parasite prevalence and crowded families, early immigrants, individuals with no social security, and people living in close contact with their livestock. The parasites were found to be less common among individuals who had been drinking bottled water and living in a house with a sewage system. The results demonstrated a correlation between the intestinal parasites and environmental conditions in our study group. We further plan to expand the study group to cover all regions of Turkey. © 2008 Springer-Verlag.
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    Prevalence and associated factors of cryptosporidium spp and cyclospora cayetanensis in Izmir Province, Turkey; [İzmir’de cryptosporidium spp ve cyclospora cayetanensis’in görülme sıklığının ve ilişkili faktörlerin araştırılması]
    (Veteriner Fakultesi Dergisi, 2012) Dirim Erdogan D.; Kurt O.; Mandiracioglu A.; Uner A.; Mucide A.K.; Dagci H.
    Cyclospora cayetanensis and Cryptosporidium spp are the two emerging coccidian parasites causing human infections by inhabiting the intestinal tract and can either remain asymptomatically, or can cause endemic and epidemic diarrhea in both children and adults. In the present study, the prevalence of C. cayetanensis and Cryptosporidium spp were assessed in an epidemiological study in Izmir province, along with risk factors related to infection. A total of 873 individuals from both the city centre and counties were included in the study, and stool samples were examined with formalin ethyl acetate and Kinyoun acid-fast stained preparations. The prevalence of C. cayetanensis and Cryptosporidium spp were found to be 3.0% and 0.8%, respectively. Lower socio-economic conditions, lack of health insurance, consumption of tap water, eating in common places were all found to be associated with higher positivity for C. cayetanensis and Cryptosporidium spp. Abdominal pain was the prominent complaint of infected individuals. Despite relatively low prevalence rates, the presence of C. cayetanensis and Cryptosporidium spp in Izmir province requires further assessments of intestinal parasites. Public health measures that may prevent the local risk factors of infection should be improved and implemented. © 2012, Veteriner Fakultesi Dergisi. All rights reserved.