Browsing by Author "Mehmet Korkmaz"

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    Kronik bor etkisinde kalan ve kalmayan kadınlarda HPV sıklığı ile genotiplerinin karşılaştırılması
    (2014) Aslı Göker TAMAY; Sinem AKÇALI; Hatice Yıldırım; SIDDIK KESKİN; tevfik guvenal; Hülya Demirci; Osman Yavuz ATAMAN; Mehmet Korkmaz
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    Evaluation of Apoptotic and Autophagic Effects of Thioridazine in Monolayer and Spheroid Cell Cultures
    (2023) Rehime; Burak ÇELİK; Mehmet Korkmaz; Ezgi ERSÖZ
    Glioblastoma (GBM) is the most frequently seen brain tumor in adults. It is clear that new therapeutic agents are needed in this area. Spheroid cell cultures have advantages on imitating in vivo environment. Therefore, we investigated apoptotitac and autophagic effects of Thioridazine (THZ) which is a potential anti-cancer agent on GBM cell lines, T98G and U-87 MG, comparatively in monolayer and spheroid cell cultures. The cytotoxic effect of THZ on cells was evaluated by MTT method. Besides, apoptotic and autophagic ef fects of THZ were determined by Annexin V and LC-3 antibody based methods. Validation performed also by assessment of apoptosis and autophagy with specific inhibitors. The analysis has revealed that IC 50 values at 24 h were 12,67 μM (T98G) and 12.80 μM (U-87 MG) in monolayer cell culture, 29.30 μM (T98G) and 28.68 μM (U -87 MG) in spheroid cell culture. While apoptotic cell rate was determined at 24 h approximate 15% in both cell lines and cell cultures, autophagy induction rate was increased by 6.5- (T98G) and 5.6- (U-87 MG) fold in monolayer cell culture and by 3.3- (T98G) and 4.5- (U-87 MG) fold in spheroid cell culture. In our study THZ stimulates mostly autophagy rather than apoptosis. In addition, it can be suggested that in vitro cytotoxicity studies should perform with not only monolayer cell cultures but also with spheroid cell cultures for obtaining in vivo-like data. When all the evaluations are considered, THZ might be thought as a potential chemotherapeutic agent in the treatment of GBM.
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    The Role of Endoplasmic Reticulum Stress in Gastroesophageal Reflux Disease Symptoms and Treatment
    (2023) Erdal BALCAN; Elmas Kasap; TAHİR BURAN; AYSUN TORAMAN; Pinar Solmaz Hasdemir; Mehmet Korkmaz; Çağdaş AKTAN
    Background: Gastroesophageal reflux disease is a common condition worldwide. There is no curative treatment for gastroesophageal reflux disease. Endoplasmic reticulum stress leads to the activation of the unfolded protein response and has an important role in inflammation. The aim is to determine the role of endoplasmic reticulum stress in the follow-up of individuals with gastroesophageal reflux disease and the temporal changes of endoplasmic reticulum stress markers with treatment. Methods: Twenty-four subjects in total were recruited prospectively, of whom 15 had nonerosive reflux disease. Two biopsies from 2 cm above the esophagogastric junction, 2 biopsies from gastric antrum mucosa, and 2 biopsies from gastric corpus mucosa were taken. Simultaneously, 2 tubes of venous blood samples were drawn from each individual (1 tube for studying the genetic markers and 1 tube for analyzing the CYP2C19 polymorphism). Results: The mean age was 42.3 ± 17.6 for women and 34.66 ± 11.2 for men. Pantoprazole, esomeprazole, rabeprazole, and lansoprazole preparations were used for treatment. There was no significant difference between tissue and blood samples for panel genes ATF-6, XBP-1, DDIT-3, DNAJC-10, and EIF-2-AK before treatment. There was a significant decrease in the level of ATF-6, XBP-1, DNAJC-9, EIF- 2-AK, and NF-2L-2 genes in blood after treatment. In the comparison of proton pump inhibitors, significant decreases in the expression of the ATF-6, XBP-1, and DNAJC-9 mRNAs were detected in blood from individuals after treatment. Conclusion: Endoplasmic reticulum stress can be for evaluating the clinical improvement and the effectiveness of treatment in gastro- esophageal reflux disease.