Browsing by Author "Meriçli F."

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    The effect of Colchicum pusillum in human colon cancer cells via Wnt/β-catenin pathway
    (Elsevier B.V., 2019) Becer E.; Hanoğlu D.Y.; Kabadayı H.; Hanoğlu A.; Vatansever S.; Yavuz D.; Meriçli F.; Meriçli A.H.
    Objective: Colchicum pusillum belongs to the family Colchicaceae that particularly rich in tropolonic alkaloids. The aim of this study was to investigate the cytotoxicity and in vitro anticancer activity of Colchicum pusillum ethanolic extract on Colo-320 primer and Colo-741 metastatic colon adenocarcinoma cell lines. Materials and methods: Colchicum pusillum was collected and extracted with ethanol. Different concentrations of Colchicum pusillum extract were incubated for 24 h and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assays. Anticancer and antiproliferative activities of Colchicum pusillum were investigated by immunocytochemistry using antibodies directed against to β-catenin, Ki-67, LGR-5 Ki-67, DKK1, Frizzled-4, Wnt4, Wnt7a and caspase3 in Colo-741 cells. Results: All concentrations of Colchicum pusillum extract had toxic effect in Colo-320 cells. Because of this, we used Colchicum pusillum extract at 20 μg/ml for evaluate anticancer activities only in Colo-741 cells. As a result of immunohistochemical staining, β-catenin, LGR-5 and caspase-3 immunoreactivities were significantly increased while Wnt7a immunostaining intensity was decreased in Colo-741 cells. Conclusion We conclude that Colchicum pusillum extract increased β-catenin and LGR-5 via Wnt/β-catenin pathway in colon cancer cells. Interestingly, it decreased other signaling molecule, Wnt7a which is assumed to play protective role during carcinogenesis. Also, it increased significantly caspase-3 immunoreactivity showing that apoptotic pathways were triggered. © 2018
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    Cellular behavior of Colchicum troodi treated primary and metastatic colon adenocarcinoma cell lines
    (Mattioli 1885, 2021) Becer E.; Yavuz D.Ö.; Vatansever H.S.; Kabadayı H.; Hanoğlu A.; Meriçli F.; Meriçli A.H.
    Colchicum troodi belongs to Colchicaceae family that particularly rich in flavonoids, phenolic acids, tannin, fatty acids and alkaloids such as colchicine. The aim of this study was to investigate the anti-proliferative and protective effects of Colchicum troodi ethanolic extract toward relevant molecular signalling pathways on Colo-320 primer and Colo-741 metastatic colon adenocarcinoma cell lines. Colchicum troodi was collected and extracted. Different concentrations of Colchicum troodi extract were incubated for 24 h and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by MTT assays. Anticancer and antiprolifetarive activities of Colchicum troodi were investigated by immunocytochemistry using antibodies directed against to β-catenin, LGR5, jagged 1, IHH, CD133 and Ki-67 in Colo-320 and Colo-741 cells. In the MTT assay, 10 µg/ml and 5 µg/ml Colchicum troodi extract were found to be active against Colo-741 and Colo-320 cells, respectively. Colchicum troodi extract significantly increased caspase β-catenin immunoreactivities while IHH immunostaining intensity was decreased in Colo-741 cells. We conclude that Colchicum troodi extract increased β-catenin via Wnt/β-catenin pathway in Colo-741 cells. Interestingly, it decreased IHH immunoreactivities which is an antagonist of constitutive activity of Wnt/ β-catenin signaling and is assumed to play protective act during carcinogenesis. © Mattioli 1885
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    Fatty acid composition of Opuntia ficus-indica seed oil control angiogenic activity in colon carcinoma cell lines
    (Mattioli 1885, 2021) Becer E.; Kabadayi H.; Meriçli A.H.; Kivançli B.; Vatansever H.S.; Meriçli F.
    Opuntia ficus-indica belongs to the family Cactaceae that particularly rich in unsaturated fatty acids. The aim of this study was to investigate the composition and in vitro anti-angiogenic activity of spiny and thornless Opuntia ficus-indica seed (CPS) oils. Spiny and thornless CPS oils were obtained by supercritical CO2 extraction and analyzed by GC-MS. Cell growth and cytotoxicity were measured with MTT assay with different concentrations of spiny and thornless CPS oils in Colo-320 and Colo-741 cell lines. Anti-angiogenic activity of CPS oils were investigated by immunocytochemistry using antibodies directed against to Flt- 1, Flk-1, eNOS, iNOS, IL-6, PGE2, TNF-α and VEGF. Oleic acid (17.6%; 15.6%), linoleic acid (55.9%; 60.1%), palmitic acid (12.4%; 12.3%), elaidic acid (4%; 4.1%) were determined as the major compounds of spiny and thornless CPS oils, respectively. In the MTT assay, 1:16 dilution spiny CPS oil was found to be active against Colo-320 and Colo-741 cells for 48h incubation. Also, 1:8 and 1:16 dilutions of thornless CPS oil were more effective at inhibiting Colo-320 and Colo-741 for 48 h, respectively.We conclude that spiny CPS oil decreased signaling molecules which play in PGE2 mediated and VEGF-dependent angiogenesis. Interestingly, thornless CPS oil increased angiogenesis thought signaling molecules in colon cancer cells. © 2021 Mattioli 1885. All rights reserved.