Browsing by Author "Nalbantoglu O.U."

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    Artificial intelligence-based personalized diet: A pilot clinical study for irritable bowel syndrome
    (Taylor and Francis Ltd., 2022) Karakan T.; Gundogdu A.; Alagözlü H.; Ekmen N.; Ozgul S.; Tunali V.; Hora M.; Beyazgul D.; Nalbantoglu O.U.
    We enrolled consecutive IBS-M patients (n = 25) according to Rome IV criteria. Fecal samples were obtained from all patients twice (pre-and post-intervention) and high-throughput 16S rRNA sequencing was performed. Six weeks of personalized nutrition diet (n = 14) for group 1 and a standard IBS diet (n = 11) for group 2 were followed. AI-based diet was designed based on optimizing a personalized nutritional strategy by an algorithm regarding individual gut microbiome features. The IBS-SSS evaluation for pre- and post-intervention exhibited significant improvement (p < .02 and p < .001 for the standard IBS diet and personalized nutrition groups, respectively). While the IBS-SSS evaluation changed to moderate from severe in 78% (11 out of 14) of the personalized nutrition group, no such change was observed in the standard IBS diet group. A statistically significant increase in the Faecalibacterium genus was observed in the personalized nutrition group (p = .04). Bacteroides and putatively probiotic genus Propionibacterium were increased in the personalized nutrition group. The change (delta) values in IBS-SSS scores (before-after) in personalized nutrition and standard IBS diet groups are significantly higher in the personalized nutrition group. AI-based personalized microbiome modulation through diet significantly improves IBS-related symptoms in patients with IBS-M. Further large-scale, randomized placebo-controlled trials with long-term follow-up (durability) are needed. © 2022 ENBIOSIS Biotechnologies Limited. Published with license by Taylor & Francis Group, LLC.
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    Culture-independent assessment of probiotic supplement consistency in commercially available probiotic supplements
    (Elsevier Ltd, 2023) Gundogdu A.; Nalbantoglu O.U.; Sarikaya I.; Karis G.; Erdogan M.N.; Hora M.; Tunali V.
    The probiotic products market has experienced significant growth in recent years, driven by increasing consumer demand for health benefits. This demand is largely fueled by the widespread promotion of probiotic products, and is compounded by relatively lax regulations regarding these products compared to those for drugs. In this study, we sought to evaluate the consistency of product labels and contents for probiotic supplements in the Turkish and USA markets. A total of 44 commercially available probiotic products were included in the analysis, comprising 40 products from Turkey and 4 from the USA. Shotgun metagenome sequencing was performed to determine the microbiological content and functional characteristics of the products. Additionally, we evaluated the presence of antibiotic-resistance genes and virulence genes in each product. Our findings showed that 38.6% of the products contained microorganisms not indicated on their labels, while 15.9% of products did not contain one or more of the probiotic microorganisms specified on their labels. Moreover, over half of the probiotic products (68.2%) were found to contain at least one resistance gene, and 40.9% of the products carried multiple virulence genes. Streptococcus thermophilus being the primary contributor to the detected virulence genes. This study is the first to examine the label and content discrepancies of probiotic products in the Turkish market and one of the largest to analyze resistance gene profiles of probiotic supplements in the literature. These findings may suggest a potential public health risk and the need for whole genome sequencing analysis of probiotic supplements. © 2023 Elsevier Ltd