Browsing by Author "Orhan, H"

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    Metal-chelated cryogels for amyloglucosidase adsorption: application for continuous starch hydrolysis
    Evli, S; Orhan, H; Aktas, PS; Uygun, M; Uygun, DA
    In the present work, a new metal-chelating platform was designed by using IDA as a chelating agent and Cu(II) as an affinity component for amyloglucosidase adsorption. Poly(AAm-GMA) cryogels were used as structural elements, while GMA monomer served reactive epoxy groups for IDA immobilization. Synthesized cryogels were characterized by FTIR, SEM and EDX studies. Pore diameter of the whole polymeric structure was 3-10 Effects of medium pH, temperature, ionic strength along with amyloglucosidase concentration were also investigated for more effective amyloglucosidase adsorption and maximum adsorbed amount of amyloglucosidase was cryogel by the optimum conditions. Reusability profile of the poly(AAm-GMA)-IDA-cryogels was also studied and it was found that the synthesized cryogels could be used repeatedly for many times without any significant decrease on their adsorption capacity. Also continuous hydrolysis of starch by using immobilized form of amyloglucosidase in a column system was studied.
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    Immunomodulatory Effects of MDA-MB-231-derived Exosome Mimetic Nanovesicles on CD4+ T Cell Line
    Oztatlici, M; Ozdemir, AT; Oztatlici, H; Kucukhuyuk, S; Ozdemir, RBO; Orhan, H; Ozbilgin, K
    Objectives: The aim of this study is to investigate the immunomodulatory effects of MDA-MB-231 cells or MDA-MB231-derived exosome-mimetic nanovesicles (NVs) on CD4+ Jurkat cells. Methods: NVs were produced by the breakdown of MDA-MB-231 cells and the characterization of generated NVs were performed by using direct-ELISA and Flow cytometry methods. We co -cultured CD4+ Jurkat cells with MDA-MB-231 cells or MDA-MB-231-derived NVs for 48 h. Subsequently, expressions of pro -inflammatory and anti-inflammatory cytokines, and related transcription factors of CD4+ Jurkat cells were evaluated by qPCR method. Results: Clustering, which is the indicator of activation, was not seen in the CD4+ Jurkat cells co -cultured with MDAMB-231 cells. However, CD4+ Jurkat cell clusters were observed in the co -culture experiments with all NV concentrations. In addition, it was determined that the expressions of pro -inflammatory cytokines significantly increased while the expressions of anti-inflammatory cytokines was dramatically decreased in the NV -treated groups. On the contrary, opposite results were obtained in CD4+ Jurkat cells co -cultured with MDA-MB-231 cells. Moreover, TNF-alpha and Gata3 expressions were decreased in all groups. Conclusion: These preliminary findings from in -vitro experiments suggested that NVs could be a potential tool in cancer immunotherapy, but our data need to be supported by more comprehensive studies.