Browsing by Author "Oz Oyar E."

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    Protective role of adrenomedullin in heterotopic ovarian transplant
    (S.O.G. CANADA Inc., 2017) Erdemoglu E.; Gürgen S.G.; Kürşat Bozkurt K.; Oz Oyar E.
    Purpose of investigation: To study adrenomedullin (ADM) in preventing ischemia and morphological changes in heterotopically transplanted ovary. Materials and Methods: Forty female Sprague-Dawley rats were divided into four groups. In groups 1 and 2 each ovary was transplanted to the corresponding inguinal region by heterotopic transplantation. In groups 3 and 4, ovaries were left intact without transplantation. Treatment was injected to left inguinal region. ADM was given in groups 1 and 3 and placebo was given in groups 2 and 4. Left ovaries showing local treatment effect in heterotopic transplantation was designated as A (1A, 2A, 3A, and 4A), right ovaries showing systemic treatment effect were designated as (1B, 2B,3B, and 4B). Main outcome measures were ischemia, follicle count, and CD 31 expression. Results: Ovaries treated with local ADM (group 1A) were in consonance with normal rat ovaries. The ovaries of rats in group 1B and placebo treated transplant group (groups 2A, 2B) exhibited varying effects of ischemia. The mean follicle numbers in groups 1A, 1B, 2A, 2B were 28 ± 3.3, 16.8 ± 2.5, 17.7 ± 2.1, 16.4 ± 2.9, respectively. The mean follicle number in groups 3A, 3B, 4A, and 4B were 27.7 ± 2.0, 28.3 ± 2.2, 28.3 ± 2.2, 27.8 ± 1.9, respectively. Corpus luteum number and CD31 expression was found to be significantly higher in group 1A. Conclusion: Subcutaneous injection of ADM to heterotopic ovarian graft site causes vasodilatation and increases angiogenesis and may protect ovarian graft against hypoxic damage.
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    The effects of melatonin and thymoquinone on doxorubicin- -induced cardiotoxicity in rats
    (Comenius University in Bratislava, 2020) Yildiz Pehlivan D.; Durdagi G.; Oz Oyar E.; Akyol S.; Ozbek M.
    OBJECTIVES: This study aims to investigate the protective effects of thymoquinone and melatonin on the heart against doxorubicin-induced cardiotoxicity in rats. BACKGROUND: Melatonin and thymoquinone may play an important role in cardiotoxicity. METHODS: The subjects were divided into four groups: Control (physiological serum on 5th day), Doxorubicin (DXR), Doxorubicin+Melatonin (DXR+MEL, 10 mg/kg melatonin, intraperitoneally), and Doxorubicin+Thymoquinone (DXR+TQ, 50 mg/kg thymoquinone, orally). On the 5th day of the experiment, all groups were injected with 45 mg/kg DXR into the tail vein. On the 8th day of the experiment, ECG recordings were performed under anaesthesia. RESULTS: Thymoquinone reduced the PR, QRS and QTc intervals, which were increased by DXR, while melatonin only reduced the QTc interval. Melatonin had a protective effect against the histopathological changes induced by DXR, while TQ did not demonstrate such an effect. DXR increased CK-MB, IL-6, MDA, IL-1, IL-18 levels and decreased SOD in the cardiac tissue. MEL reduced the levels of CK-MB, MDA, NO, SOD, IL-1, IL-6, IL-18. Meanwhile, TQ only reduced CK-MB, IL-1 and IL-18. CONCLUSION: Our study showed that DXR induces cardiac injury and that melatonin improves biochemical parameters and offers histological protection; while thymoquinone improves ECG parameters and causes partial recovery of biochemical parameters (Tab. 4, Fig. 2, Ref. 41). Text in PDF www.elis.sk. © 2020.