MCBÜ Açıkerişim Sistemi :: Browsing by Author "Ozcakir, HT"

Browsing by Author "Ozcakir, HT"

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Mode of delivery and pelvic floor muscle strength and sexual function after childbirth
Baytur, YB; Deveci, A; Uyar, Y; Ozcakir, HT; Kizilkaya, S; Caglar, H
Objective: To investigate the respective roles of the mode of delivery and strength of pelvic floor muscles in the sexual function of women. Method: Thirty-two women who were delivered vaginally and 21 women who underwent cesarean delivery at the Celal Bayar University School of Medicine Obstetrics Department were enrolled in the study, and 15 nulliparas were recruited as controls. Sexual function was assessed in all women by a validated questionnaire (the Female Sexual Function Index). Desire, arousal, lubrication, orgasm, satisfaction, and pain were measured separately, and pelvic floor muscle strength was assessed by a perineometer. Sexual function was compared among the 3 groups. The correlation between pelvic floor muscle strength and sexual function was also investigated. Results: Pelvic floor muscle strength was significantly lower in the group vaginally delivered compared with the group delivered by cesarean section and the nulliparous group (P < 0.05). There was no difference between the groups regarding sexual function (P > 0.05), and there was also no correlation between sexual function and pelvic muscle strength. Conclusion: Pelvic floor muscle strength and mode of delivery did not affect sexual function in our study participants. The muscular component of female sexual function should be further investigated. (c) 2005 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.
Hormone receptor expressions and proliferation markers in postmenopausal endometrial polyps
Inceboz, US; Nese, N; Uyar, Y; Ozcakir, HT; Kurtul, O; Baytur, YB; Kandiloglu, AR; Caglar, H; Fraser, IS
Background/Aims: Endometrial polyps are quite common in the general population, they have a significant role in postmenopausal bleeding, and the pathogenesis is unclear. The aim of this study was to investigate proliferation markers and expression of estrogen and progesterone receptors in endometrial polyps in postmenopausal women. Methods: Endometrial polyps were removed by hysteroscopy from 36 women who presented with postmenopausal bleeding. None were using hormonal therapy. The control group consisted of 16 inactive-atrophic postmenopausal endometrial specimens removed at hysterectomy. Immunohistochemistry was used to demonstrate expression of estrogen and progesterone receptors and the cell growth and apoptosis markers, Ki67, bcl-2, c-erbB-2. Results: In both the glandular epithelium and stroma of endometrial polyps, estrogen and progesterone receptors, Ki67 and bcl-2 showed significantly more positive staining than the inactive endometrium from the control group. There was no difference in expression of c-erbB-2 between the two groups. Conclusions: Estrogen may have a role in the development of postmenopausal endometrial polyps, either by direct stimulation of localized proliferation or by stimulation of proliferation via other pathways, such as activation of Ki67 or through inhibition of apoptosis via bcl-2. c-erbB-2 is unlikely to play any role in development of these lesions. Copyright (C) 2006 S. Karger AG, Basel.
Evaluation of female athlete triad and gynecological complaints in young Turkish female athletes
Hasdemir, PS; Oral, O; Calik, E; Ulusoy, M; Varol, R; Ozcakir, HT
Purpose: Female athlete triad (FAT) is a serious healthcare concern in the young female population. The aim of this study was to determine the prevalence and clinical characteristics of FAT and its relationship with gynecologic complaints of young Turkish female athletes. Materials and Methods: This was a cross-sectional survey and included 87 female athletes involved in a variety of sports as a case group and 85 sedentary female university students from Ege and Celal Bayar Universities. All subjects completed a questionnaire consisting of 32 separate questions assessing eating behavior, menstrual status, gynecologic and systemic complaints, psychological problems, and sexual history. SPSS 21.0 was used for statistical analysis. Results: This survey involved 172 females in between to 30 years of age. The age of menarche was found to be significantly lower in the sedentary group (p = 0.00). Late menstruation and oligo-amenorrhea were notable complaints among the athletes, and body mass index (BMI) was found to be statistically lower in the athletic group (p = 0.00). Cold hands and hair loss were seen more often in the sedentary group compared to the athletic group (p < 0.05). According to a logistic regression analysis, independent variables (BMI, menstrual status, and bone fractures) were found to be statistically insignificant (p > 0.05). Conclusions: Excessive sports activity can be hazardous in young female population. Lower BMI might be related to menstrual irregularity. Young female population should be informed about this relationship, especially athletes who are particularly at risk. Certain precautions should be taken into consideration in this population in order to get benefits of sports activity.
Endometriosis associated with relapsing ascites and pleural effusions
Hasdemir, PS; Ikiz, N; Ozcakir, HT; Kara, E; Guvenal, T
Evaluation of VEGF in placental bed biopsies from preeclamptic women by immunohistochemistry
Cirpan, T; Akercan, F; Terek, MC; Kazandi, M; Ozcakir, HT; Giray, G; Sagol, S
Objective: The aim of the study was to determine VEGF protein with immunohistochemical staining in placental bed biopsies of preeclamptic pregnancies in comparison to normal controls. Design: Prospective cohort Study. Methods: The placental bed biopsies were obtained from 12 patients with preeclapmsia and ten patients for a control group at the time of cesarean delivery. Tissue samples of the placental bed were examined for VEGF protein distribution with avidin-biotin-peroxidase immunohistochemistry. Two blinded histopathologists were asked to score each sample for the intensity of staining and the number of cells stained in a randomly selected HPF of each sample. The resulting H-score was computed as a product of intensity and percent of cells stained. Results: VEGF expression was significantly lower in both the myometrium and stroma of the preeclamptic group compared to the control group (77.2 +/- 25.4 vs 134 +/- 44.3, p = 0.007; 194.1 +/- 20.7 vs 170.2 +/- 17, p = 0.017, respectively). Conclusion: VEGF expression is significantly lower in placental bed biopsies of preeclamptic pregnancies.
The immunohistochemical evaluation of VEGF in placenta biopsies of pregnancies complicated by preeclampsia
Akercan, F; Cirpan, T; Terek, MC; Ozcakir, HT; Giray, G; Sagol, S; Karadadas, N
Objective The study was designed to determine the protein levels of vascular endothelial growth factor (VEGF) in the placenta biopsies of patients with preeclampsia and compare with normal controls. Design Prospective cohort study. Methods The placental biopsies were obtained from ten patients with preeclampsia and ten patients of control group at the time of delivery. Avidin-biotin-peroxidase immunohistochemistry was then performed to identify levels of VEGF protein within the tissue and a semi-quantitative method was devised to score the amount of staining present in the sample. Two histopathologists who were blinded to the groups were asked to score each sample for the intensity of staining and the number of cells stained in a randomly selected per high-power fields of each sample. The resulting H-score was computed as a product of intensity and percent of cells stained. Results The VEGF expression was significantly higher in placenta biopsies of preeclamptic patients compared to that of controls (271.2 +/- 22.65 vs. 201.9 +/- 12.33, P = 0.000). Conclusion Immunostaining of VEGF is significantly higher in placenta biopsies of patients with preeclampsia.
Assessment of fetal cerebral arterial and venous blood flow before and after vaginal delivery or Cesarean section
Baytur, YB; Tarhan, S; Uyar, Y; Ozcakir, HT; Lacin, S; Coban, B; Inceboz, U; Caglar, H
Objectives To compare perinatal intracranial arterial and venous blood velocity changes between healthy term neonates delivered vaginally or by Cesarean section and to correlate these changes with cord blood gases and pH values at birth. Methods The study involved 43 healthy term neonates who were delivered vaginally (n = 20) or by Cesarean section (n = 23). All fetuses/neonates were examined by Doppler ultrasound to obtain middle cerebral artery (MCA) and cerebral transverse sinus (Tsin) Doppler waveforms on three occasions (before delivery, and 1 h and 24 h after birth). Pulsatility index (PI) and peak systolic velocity (PSV) for MCA and Tsin were measured and compared between neonates who were delivered vaginally or by Cesarean section. Umbilical cord blood samples were analyzed for umbilical artery and vein pH, pO(2) and pCO(2) and values were correlated with MCA and Tsin Doppler indices. Results MCA-PI increased and MCA-PSV decreased at 1 h after birth, and Doppler measurements returned to predelivery values at 24 h after birth. Tsin Doppler measurements remained unchanged at 1 h and 24 h when compared to predelivery values in both the Cesarean and vaginal delivery groups. There was a negative correlation between Tsin-PI before birth and umbilical venous pH. There was a positive correlation between Tsin-PSV at 1 h after birth and umbilical vein PCO2. Conclusions Cerebral arterial blood velocity decreases immediately after birth and increases within 24 h, probably as part of neonatal adaptation. Cerebral venous blood velocity remains constant during the perinatal period and is likely to be regulated in a different and more complex manner than that of arterial blood velocity. Mode of delivery does not affect cerebral blood velocity. Copyright (C) 2004 ISUOG. Published by John Wiley Sons, Ltd.
Endometrial thickness and the Doppler sonographic parameters of the uterine arteries as discriminators of endometrial status in postmenopausal women receiving anti-hypertensive treatment
Ozcakir, HT; Inceboz, US; Utuk, O; Baytur, YB; Caglar, H
Aim: The aim of the present study was to establish the Doppler sonographic parameters of the uterine arteries in postmenopausal patients with or without hypertension and to determine the value of their measurement in the prediction of endometrial pathology. Methods: Healthy postmenopausal women (n = 23) and the ones receiving anti-hypertensive medication (n = 34) examined for both endometrial thickness and Doppler velocimetry of the uterine arteries by transvaginal sonography. Results: There was no sigificant difference between endometrial thickness and uterine artery Doppler sonographic parameters in hypertensive postmenopausal women compared to normotensive controls. Conclusion: The role of Doppler examination in the differential diagnosis of endometrial pathology in patients with or without hypertension seemed not to be effective.
Effect of GnRH antagonist therapy on the expression of MUC-1 and heparin binding growth factor expression in the endometrium of hyperstimulated rats
Ozcakir, HT; Taman, AG; Kose, C; Ozbilgin, K; Inan, S; Caglar, H
Purpose: To determine the effect of GnRH-antagonist therapy on the expression of heparin binding-epidermal growth factor (HB-EGF) and MUC-1 glycoprotein in hyperstimulated rat ovaries. Methods: 30 female Wistar rats were divided into three groups (control, FSH and FSH+cetrorelix). Control rats were given 0.2 ml oil/saline mixture for four days beginning from the day of estrus. In the second group, 30 IU/ml purified hFSH was injected SC for four days beginning from the day of estrus. The rats of the third group were injected 30 IU FSH for four days and 10 IU cetrorelix SC for three or four days. The rats were sacrificed and the staining intensity of HB-EGF and MUC-1 of the epithelial cells and stromal cells of the endometrium of the rats was calculated by H-score. Results: Slight MUC-1 immunoreactivity was seen in the epithelial and decidual cells of the control and FSH groups. In the FSH+cetrorelix group, moderate MUC-1 immunostaining appeared in the epithelial and desidual cells. In rats in the control and FSH+cetrorelix groups, HB-EGF immunoreactivity in the epithelial cells and decidual cells was moderate. Strong immunoreactivity was seen in the FSH group. When the MUC-1 H-score values were compared statistically with the control and other groups, FSH+cetrorelix immunoreactivity in epithelial and decidual cells were significantly different from control and FSH groups. HB-EGF immunoreactivity of the epithelium and decidua was similar in the control and FSH+cetrorelix groups, but epithelial and decidual immunoreactivity of the FSH group was different from the other two groups. Conclusion: Our findings suggest that GnRH antagonists exert direct effects on the expression of HB-EGF and MUC-1 expression in the rat endometrium.
A Comparison of Success Rates of Embryo Transfer on Weekdays and Weekends
Hasdemir, PS; Kamali, MB; Calik, E; Ozcakir, HT
Background: The aim of this study is to examine the effect of the embryo transfer (ET) day on clinical pregnancy success rates in in vitro fertilization-ET (IVF-ET) cycles. Materials and Methods: In this retrospective study, we divided patients with infertility who underwent IVF-ET with fresh embryos into two groups depending on whether the ET was performed on weekdays or weekends. The main outcome measure was to compare the clinical pregnancy rates of patients with similar demographic and clinical characteristics who underwent ET on weekdays or weekends. Results: A total of 188 patients underwent IVF-ET on weekdays (n=156) or weekends (n=32). Both groups had similar demographic and cycle characteristics. The overall pregnancy rate was 42.8%. Among the study groups, the weekday group had a 40.2% ET success rate and the weekend group had a 54.8% success rate (P=0.517). Although no statistically significant difference existed between the two groups, we observed an absolute 14.6% increase in pregnancy rate for ETs performed during weekends compared to those performed on weekdays, with a 35% statistical power. Conclusion: ETs performed during weekends were more successful than ETs performed during weekdays with an absolute 14.6% increase in clinical pregnancy rate. This finding should be confirmed by conducting further studies with larger groups of patients.
Effect of angiotensin-converting enzyme-inhibiting therapy on the expression of vascular endothelial growth factor in hyperstimulated rat ovary
Ozcakir, HT; Giray, SG; Ozbilgin, MK; Inceboz, US; Caglar, H
Objective: To determine the effect of angiotensin-converting enzyme-inhibiting therapy on the expression of vascular endothelial growth factor (VEGF) in the hyperstimulated rat ovary. Design: Experimental study. Setting: University animal research laboratory. Animal(s): Thirty Wistar albino adult female rats were studied; 20 rats were stimulated with gonadotropins (groups 1 and 2), and 10 were controls (group 3). Ten of the stimulated rats received additional treatment with enalapril (group 2). Intervention(s): At the end of the treatment period, rat ovaries were subjected to immunohistochemical staining with anti-VEGF antibodies. Main Outcome Measure(s): VEGF staining intensity was graded semi quantitatively, and the H-score was calculated by light microscopic examination of the groups. Result(s): VEGF expression was found to be significantly higher in the endothelium and stroma in groups 1 and 2 compared with group 3. Although VEGF immunoreactivity was lower in the stimulation regimen plus enalapril group compared with the stimulation regimen-only group, the difference was insignificant. Conclusion(s): Enalapril does not seem to have a significant effect on VEGF expression in the hyperstimulated rat ovary. Because angiotensin 11 exerts its multiple actions via specific receptors, there may be other factors, such as a receptor blockade, that contribute to the VEGF expression. (C) 2004 by American Society for Reproductive Medicine.
Immunohistochemical detection of transforming growth factor-α, epidermal growth factor, and vascular endothelial growth factor expression in hyperstimulated rat ovary
Ozcakir, HT; Giray, SG; Ozbilgin, MK; Uyar, Y; Lacin, S; Caglar, H
Objective. The aim of the present study is to figure out the immunohistochemical expression of transforming growth factor-alpha (TGF-alpha), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF) in hyperstimulated rat ovaries. Methods. Twenty Wistar-Albino adult female rats (250-300 g) were taken into the study. The animals were randomly divided into two groups, each containing 10 rats: (i) stimulation group and (ii) control group. In the stimulation group, a stimulation regimen was administered to induce follicular maturity and ovarian hyperstimulation syndrome (OHSS) at the end using a 30-IU follicle-stimulating hormone that was administered subcutaneously for 4 consecutive days, followed by a 30-IU human chorionic gonadotropin on day 5 to induce ovulation. The rats, in the control group, received 0.2 ml of 0.9% NaCl for 5 consecutive days to mimic the conditions of the study animals. At the end of the treatment period, all rats underwent ovariectomy and the sections of ovaries were stained for the TGF-alpha, EGF, and VEGF. Results. The expression of TGF-alpha, EGF, and VEGF in the endothelium, the stroma, the granulosa cells, and the corpus luteum was found to be significantly higher in the stimulated group, compared to that in the control group ( p < 0.05). Conclusion. TGF-alpha, EGF, and VEGF are found to have increased in the hyperstimulated ovaries and this finding seems to be involved in the OHSS pathogenesis.

Browsing by Author "Ozcakir, HT"

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