Browsing by Author "Ozdal Kurt F."

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Inhibitory effects of propolis on human osteogenic sarcoma cell proliferation mediated by caspase patway; [Kaspaz yolaǧı ile yönlendirilen i̇nsan osteojenik sarcoma hücre çoǧalmasına propolisin baskılayıcı etkisi]
    (KAFKAS UNIVERSITY, 2010) Ozdal Kurt F.; Vatansever H.S.; Sorkun K.; Deliloǧlu Gurhan S.I.; Turkoz E.; Gencay O.; Salih B.
    A natural product Propolis, is a resinous material gathered by honeybees from the buds and bark of certain trees and plants. Propolis contains various chemical components of biological activities, including antimutagenic, antioxidant, antibacterial, antiviral and anticarsinogenic. Therefore, the aim of this study is to investigate the antiapoptotic effect of propolis extracts (PE) using caspase pathway in the human osteogenic sarcoma cell line SAOS-2 in culture. The extracts which produced in ecologic environment were taken from the Hacettepe University, Beytepe Campus area-Ankara were used. Seven different PE at 0.5, 0.25, 0.125 and 0.063 mg/ml were added to SAOS-2 cell line for two days incubation. For cell proliferation and cytotoxicty analyses MTT, for apoptotic cell death determination TUNEL method, for distribution of caspase 6, caspase 8 and caspase 9 indirect immunocytochemistry analyses were used. After MTT analyses, the most effect was observed PE 7 at the 0.125 mg/ml dilution. The number of TUNEL positive cells was more detectable at PE 4 and 5 at the 0.063 mg/ml, and PE 7 at the 0.125 mg/ml dilutions. The immunoreactivity of caspase 6 was stronger than caspase 8 and 9. Moreover, density of caspase 6 staining was much better especially in PE 7 at the 0.125 mg/ml dilution. In conclusion, the mechanisms of apoptosis induction by PE may appear via caspase pathway because of its anticanserogenic effect. PE may be usefull in the cancer treatment protocol.
  • No Thumbnail Available
    Item
    Apoptosis of colon cancer cells under the effect of geldanamycin derivate
    (Comenius University, 2017) Kosova F.; Kasar Z.; Tuglu I.; Ozdal Kurt F.; Gok S.; Ari Z.; Imren T.
    AIM: The apoptotic effect of geldanamycin derivative may be important for the colorectal cancer therapy. The mechanisms of apoptosis require understanding of the behavior of colon cancer cell line Colo-205 which mimics colon adenocarcinoma. Therefore, the effect of IC50 dose of 17-allylamino-17-demethoxygeldanamycin (17- AAG) on the colon cancer cells in vitro was studied for its anti-apoptotic activity. METHOD: Apoptotic ratio of the Colo-205 cells was determined after 17-AAG application with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and apoptosis related genes. Apoptosis signal path related key mitochondrial proteins, cytochrome c, bcl-2, caspase 9 and Apaf-1 expression were examined with RT-PCR method. RESULTS: 17-AAG caused induction of cell death. Apoptotic related genes such as cytochrome-c, Apaf-1 and caspase-9 protein expressions were increased signifi cantly (p < 0.05) and anti-apoptotic bcl-2 expression was decreased signifi cantly (p < 0.05). Our results indicated that the application of 17-AAG on Colo-205 cells showed anticancer effect by the apoptosis due to alteration of apoptotic genes. CONCLUSION: The apoptotic effect of 17-AAG as an natural product for alternative medicine would be very important for the success and quality of life during the treatment of colon carcinoma with the combination of anticancer drugs.