Browsing by Author "Ozel N."

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    Exploring deep eutectic solvents for enhanced extraction of bio-active compounds from microalgae biomass
    (Elsevier B.V., 2024) Ozel N.; Inam A.; Elibol M.
    Microalgae biomass contains proteins, polysaccharides, carotenoids, phenolic compounds, vitamins, minerals, and other valuable compounds. In the extraction process of useful intracellular products from biomass, deep eutectic solvents (DESs) offer several advantages as an alternative to organic solvents. This study used deep eutectic solvents as extraction media to obtain bio-active compounds from freeze-dried microalgae biomass. This study aimed to evaluate the effect of two different DESs (Choline chloride: acetic acid (1:2) (CCA) and Choline chloride: urea (1:2) (CCU)) on the extraction of lipids, carotenoids, proteins, phenolic compounds, and carbohydrates from different microalgae biomass including Schizochytrium sp., Chlorella vulgaris, Scenedesmus protuberans and Neochloris texensis. The best results were obtained as follows: proteins from CCA-N. texensis (28.99 mg/g DW), carotenoids from CCA-S. protuberans (33.55 mg/g DW), phenolics from CCA-S. protuberans (33.44 mg GAE/g DW), carbohydrates from CCA-S. protuberans (18.32 mg/g DW), and lipids (the treated biomass pellets) from CCU-N. texensis (18.3 %). UV-spectrophotometry, LC–MS/MS, and GC–MS were used to analyse the carotenoid, phenolic, and lipid content, respectively. © 2024 Elsevier B.V.
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    Silver nanoparticles produced from Agropyron repens: A green synthesis approach and antimicrobial analysis
    (Field Crops Central Research Institute, 2025) Inam A.; Ozel N.; Gunay Z.; Elibol M.
    The plant Agropyron repens is invasively distributed both in natural grassland areas and in agricultural areas. It contains valuable metabolites such as carbohydrates, phenolic compounds, flavonoids, saponins, essential oils, and minerals. A. repens plant extract has the potential as an alternative source for nanoparticle synthesis. The total phenolic and carbohydrate contents of the aqueous extract were analyzed using spectrophotometric methods. Silver nanoparticles (AgNPs) were synthesized at room temperature using the aqueous extract of A. repens without any chemical stabilizer or reducing agent. The formation of AgNPs was confirmed by the observed color change. UV-Vis spectroscopy, dynamic light scattering (DLS), Fourier transform infrared spectroscopy, and X-ray diffraction were used for characterization. An absorption was obtained in the UV-Vis spectrum. In DLS measurements, the size of AgNPs was determined to be approximately 75 nm. The obtained AgNPs and fabrics treated with these nanoparticles showed antibacterial activity against both gram positive (Staphylococcus aureus) and gram negative (Escherichia coli) bacteria. AgNPs and fabric samples exhibited antifungal activity against Candida albicans. In addition, the antioxidant activities of aqueous extracts and AgNPs were evaluated. This study highlights that A. repens can be used in the production of AgNPs by green synthesis methods. © 2025, Field Crops Central Research Institute. All rights reserved.