MCBÜ Açıkerişim Sistemi :: Browsing by Author "Ozer, MS"

Browsing by Author "Ozer, MS"

Now showing 1 - 20 of 26

Can the stalks of Papaver somniferum L. be an alternative source of bioactive components?
Kirkan, B; Ozer, MS; Sarikurkcu, C; Copuroglu, M; Cengiz, M; Tepe, B
Alternative raw materials for designing novel products are gaining interest in the scientific area. The aim of this study is to investigate the antioxidant and enzyme inhibitory activities of the extracts obtained from Papaver somniferum L. stalks as well as the determination of chemical composition. Antioxidant capacity was tested by using different chemical methods including radical scavenging, reductive effect, phosphomolybdenum and metal chelating assays. Enzyme inhibitory effect was investigated against cholinesterase, tyrosinase, alpha-amylase and alpha-glucosidase. To detect chemical composition, phenolic profile was determined by high performance liquid chromatography (HPLC) as well as total contents of the phytochemicals. The aqueous extract was found to contain high amount of phenolics and flavonoids [9.87 mu mol gallic acid equivalents (GAEs)/g dry plant (dp) and 0.09 mu mol rutin equivalents (REs)/g dp, respectively]. The methanol and aqueous extracts were also found to contain considerable amounts of (-)-epicatechin and syringic acid. Antioxidant activity tests were resulted in the superiority of the methanol extract. The extracts did not show inhibitory activity on cholinesterases and tyrosinase. Inhibitory activity of the aqueous extract on alpha-amylase and alpha-glucosidase were found to be 9.85 and 78.64 mu mol acarbose equivalents (ACEs)/g dp, respectively. As a result, P. somniferum stalks, one of the major by-products of poppy cultivation, can be used as the alternative source of antioxidant and enzyme inhibitory compounds in food and medical industries.
Essential Oil Composition and Antioxidant Activities of Alkanet (Alkanna tinctoria subsp. tinctoria)
Ozer, MS; Sarikurkcu, C; Tepe, B; Can, S
This study is designed to examine the chemical composition and in vitro antioxidant activity of the hydrodistillated essential oil and the various extracts of alkanet (Alkanna tinctoria subsp. tinctoria). Gas chromatography (GC) and GC-mass spectrometry (GC-MS) analysis of the essential oil were resulted in the determination of 27 different compounds, representing 93.32% of the total oil. The major compounds detected in the oil, were pulegone (22.27%), 1,8-cineole (13.03%), alpha-terpinyl acetate (6.87%), and isophytol (6.83%), respectively. Antioxidant activities of thy samples were determined by 4 different test systems namely beta-carotene/linoleic acid, 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power, and chelating effect. In all systems, essential oil showed the weakest activity profile. On the other hand, ethyl acetate and water extracts exhibited excellent antioxidant activities. As well as the antioxidant activities of the extracts, they were evaluated in terms of their total phenolic and flavonoid contents. In parallel to the experiments, ethyl acetate and water extracts were found to be rich-in these phytochemicals.
Comparison of methanolic extracts of Doronicum orientale and Echium angustifolium in terms of chemical composition and antioxidant activities
Arumugam, R; Sarikurkcu, C; Ozer, MS
Medicinal plants have been a priceless source of medication in nearly all cultures and societies worldwide due to the presence of phytochemicals and their wide range of therapeutic potentials including antioxidants. In this study, the methanolic leaf extracts of Doronicum orientale and Echium angustifolium were screened for antioxidant activity and quantitative phytochemical analysis using LC-ESI-MS/MS. The study revealed that the D. orientale exhibited significantly highest total phenolic content as well as highest antioxidant activity in all tests. The quantitative phytochemical analysis using chromatographic analysis revealed significantly high content of hesperidin, chlorogenic acid, hyperoside, protocatechuic acid, gallic acid and quercetin in D. orientale, while E. angustifolium had rosmarinic acid, 4-hydroxybenzoic acid, p-coumaric acid, caffeic acid, vanillic acid, pinoresinol, syringic acid and luteolin-7-glucoside in higher contents. The results showed that the methanol extract of D. orientale could be an alternative source of natural agents in the management of the oxidative stress.
A comprehensive study on chemical composition, antioxidant and enzyme inhibition activities of the essential oils of Chenopodium botrys collected from three different parts of Turkey
Ozer, MS; Sarikurkcu, C; Ceylan, O; Akdeniz, I; Tepe, B
In this study, we aimed to evaluate the chemical composition of the essential oil of Chenopodium botrys L. collected from three different parts of Turkey. Additionally, we analyzed the antioxidant activities of the oil samples by using free radical scavenging, phosphomolybdenum, ferrous ion chelating, and reducing power assays as well as their inhibitory activities on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase. According to the results of GC-FID and GC MS analyses, twenty-seven, twenty-four, and sixteen compounds were identified representing 94.45%, 96.96%, and 94.41% of the oils, respectively. In Afyonkarahisar and Isparta samples, ledol (25.11% and 22.73%), elemol (15.25% and 24.86%), and germacrene D-4-ol (14.83% and 11.27%) were determined as the major compounds, whereas ledol (31.83%), elemol (23.70%), and eudesm-7(11)-en-4-ol (8.94%) were found as the main compounds of Konya sample. The oil of Isparta sample showed the maximum radical scavenging activity in all test systems, except superoxide anion radical scavenging assay. Phosphomolybdenum, ferrous ion chelating, and reducing power assays were resulted in the superiority of Afyonkarahisar sample. In AChE and tyrosinase inhibition assays, the essential oil of C. botrys collected from Konya showed the maximum activity (0.87 mg GALAEs/g oil and 0.82 mg KAEs/g oil, respectively). On the other hand, BChE inhibition assay was resulted in the superiority of Afyonkarahisar sample (1.02 mg GALAEs/g oil). In order to determine the contribution of oil components to the biological activity, correlation coefficients between the compounds and assays were also presented.
Comparison of the Influence of the Solvent on the Extraction of the Bioactive Compounds from Marrubium lutescens Using Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS/MS)
Sarikurkcu, C; Ozer, MS; Tlili, N
To our knowledge, this is the first study on the influence of water, ethyl acetate and methanol as the extraction solvent upon the bioactive compound composition, antioxidant capacities and enzyme inhibitory activities of Marrubium lutescens isolates. The contents of the total phenolic compounds varied between 9.94 and 54.80 mg gallic acid equivalent/g extract and the level of total flavonoids were between 4.45 and 27.20 mg quercetin equivalent/g extract. Twenty-four compounds were identified using liquid chromatography - electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Rosmarinic acid, luteolin 7-glucoside, chlorogenic acid, caffeic acid and hyperoside were the primary compounds present for all of the used solvents. The most compounds (23) were detected in the methanol extract. Six antioxidant assays revealed that M. lutescens extracts showed interesting antioxidant activities with a significant variation between the extracts. The results revealed that the highest tyrosinase and alpha-amylase inhibitory activities was detected in the ethyl acetate extract. This work confirmed that the nature of solvent influenced the levels and the types of the extracted biomolecules. Moreover, the obtained results suggest that M. lutescens may be a source of bioantioxidants for industrial additives.
Chemical characterization and biological activity of Onosma gigantea extracts
Sarikurkcu, C; Kirkan, B; Ozer, MS; Ceylan, O; Atilgan, N; Cengiz, M; Tepe, B
Onosma species have traditionally been used as laxative and anthelmintic agents as well as their uses in eye, blood diseases, bronchitis, abdominal pain, strangury, thirst, itch, leucoderma, fever, wounds, burns, piles, and urinary calculi. This study examines the investigation of biological activities of ethyl acetate, methanolic, and aqueous extracts from Onosma gigantea Lam. The extracts were evaluated for the antioxidant and enzyme inhibitory activities. Phenolic compounds in the extracts were also detected by RP-HPLC-DAD. The aqueous extract showed remarkable antioxidant activity in phosphomolybdenum [134.31 mu mol trolox equivalents (TEs)/g air dry matter (adm)], chelating effect [32.97 mu mol ethylenediaminetetraacetic acid (disodium salt) equivalents (EDTAEs)/g adm], radical scavenging [on DPPH (32.14 mu mol TEs/g adm) and ABTS (58.68 mu mol TEs/g adm)), and reducing power [CUPRAC (50.23 mu mol TEs/g adm) and FRAP (40.96 mu mol TEs/g adm)] assays. The aqueous extract also exhibited considerable inhibitory activity of tyrosinase (10.48 mu mol kojic acid equivalents/g adm) and alpha-glucosidase [1320.53 mu mol acarbose equivalents (ACES)/g adm]. It has been determined that the methanolic extract showed significant AChE (31.57 mu mol galanthamine equivalents (GALAEs)/g adm) and alpha-amylase (6.75 mu mol ACEs/g adm) inhibitory activity. The ethyl acetate, which contains low polarity compounds, showed the highest BChE inhibitory activity (6.87 mu mol GALAEs/g adm). In general, it is found that there is a positive correlation between the phytochemical contents of the extracts and their biological activities. The results indicate that O. gigantea extracts could be considered as a novel source of bioactive agents with functional connections.
Chromatographic profile and antioxidant and enzyme inhibitory activity of Sideritis leptoclada: An endemic plant from Turkey
Sarikurkcu, C; Ozer, MS; Istifli, ES; Sahinler, SS; Tepe, B
Sideritis species have been used for centuries for their antimicrobial, digestive, anti-ulcerogenic and anti-inflammatory properties. Therefore, in this study, it was aimed to evaluate the chemical composition, antioxidant and enzyme inhibitory activities of solvent extracts of S. leptoclada O. Schwarz & P.H. Davis, an endemic species. It was determined that methanol (MeOH) and water extracts contained high amounts of chlorogenic acid and verbascoside, while the ethyl acetate (EtOAc) extract contained high amounts of 4-hydroxybenzoic and vanilic acids. The water extract showed the highest antioxidant activity in almost all parameters. Inhibitory activity of the MeOH extract on tyrosinase was 1.04 mg/ml, which was the highest value in this experiment. On the other hand, in alpha-amylase inhibitory assay, the EtOAc extract showed an activity of 2.21 mg/ml. Docking analysis showed that verbascoside can be an effective tyrosinase and alpha-amylase inhibitory agent. (C) 2021 SAAB. Published by Elsevier B.V. All rights reserved.
Phenolic composition, antioxidant and enzyme inhibitory activities of acetone, methanol and water extracts of Clinopodium vulgare L. subsp vulgare L.
Sarikurkcu, C; Ozer, MS; Tepe, B; Dilek, E; Ceylan, O
The aim of this study is to evaluate the antioxidant and enzyme inhibitory activities of acetone, methanol and water extracts of Clinopodium vulgare L. subsp. vulgare L. as well as its phytochemical composition. Methanol extract was found rich in of phenolic compounds (44.42 mg GAEs/g extract) whereas the highest flavonoid content was determined in the water extract (40.20 mg REs/g extract). Methanol extract was also found rich in protocatechuic acid, (+)-catechin, chlorogenic acid, caffeic acid, ferulic acid, rosmarinic acid and apigenin. Data obtained from the phosphomolybdenum assay was found in correlation with those obtained from total phenolic assay. Methanol extract exhibited the highest activity (374.86 mmol TEs/g extract). In the case of chelating effect assay, acetone extract exhibited the highest activity. As observed in many previously reported studies, water extract exhibited the highest DPPH free radical scavenging activity (81.72 mg TEs/g extract). ABTS cation scavenging assay was resulted in the superiority of methanol extract (51.45 mg TEs/g extract). It also showed the strongest reducing power in CUPRAC and FRAP assays. Acetone extract exhibited remarkable inhibitory activity on acetylcholinesterase, butyrylcholinesterase and tyrosinase, while the highest a-amylase and a-glucosidase inhibitory activities were exhibited by methanol and water extracts. Total amounts of unsaturated fatty acids were determined as 51.15%. C18:1 omega 9, C18:2 omega 6 and C18:3 omega 3 were determined in considerable quantities (23.93, 6.18 and 5.78%, respectively). (C) 2015 Elsevier B.V. All rights reserved.
Enhanced optical oxygen sensing using a newly synthesized ruthenium complex together with oxygen carriers
Ertekin, K; Kocak, S; Ozer, MS; Aycan, S; Cetinkaya, B
In this article, an emission based, simple and fast method is proposed for the determination of gaseous oxygen. A newly synthesized fluorophore dichloro-{2,6-bis[1-(4-dimethylamino-phenylimino) ethyl]pyridine} ruthenium(II) has been used for oxygen sensing together with oxygen carrier perfluorochemicals (PFCs) in silicon matrix. It should be noted that the solubility of oxygen in fluorocarbons is about three to ten times large as that observed in the parent hydrocarbons or in water. respectively. Employed PFCs are chemically and biochemically inert, have high dissolution capacities for oxygen. and, once doped into sensing film, considerably enhance the response of sensing agent. (C) 2003 Elsevier B.V. All rights reserved.
Essential oil composition and antioxidant activity of endemic Marrubium parviflorum subsp oligodon
Sarikurkcu, C; Ozer, MS; Calli, N; Popovic-Djordjevic, J
In this study, chemical composition and antioxidant activity of the essential oil and solvent extracts (hexane, dichloromethane, ethyl acetate, methanol and water) obtained from aerial parts of the endemic plant Marrubium parviflorum subsp. oligodon collected from Afyonkarahisar province in Turkey were examined. The antioxidant ability of solvent extracts and essential oil was evaluated using various methods (beta-carotene-linoleic acid, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, and reducing power assays) and the content of total phenolics and flavonoids were determined. In the essential oil, thirty-one compounds were identified by GC/MS analysis. The major components were (Z,Z)-farnesyl acetone (19.28%), caryophyllene oxide (15.85%) and pulegone (7.15%). In all assays, water extract showed the strongest antioxidant properties due to the highest content of total phenolics. The significantly high correlations were observed between the content of total phenolics and DPPH radical (0.894) and reducing power (0.983) assays. Methanol extract was the richest in total flavonoids. The present results support the traditional and possible use of the essential oil and extracts from M. parviflorum subsp. oligodon in food, pharmaceutical, and cosmetic industries.
Valeriana dioscoridis aerial parts' extracts - A new source of phytochemicals with antioxidant and enzyme inhibitory activities
Sarikurkcu, C; Jeszka-Skowron, M; Ozer, MS
Valeriana root extracts are used in herbal medicine for treating sleep disorders. There is a lack of information of phytochemical composition of Valeriana dioscoridis aerial parts' extracts. The aim of the study was to measure antioxidant activity, determine the phenolic compounds from different groups such as phenolic acids and polyphenolic compounds: verbascoside or eriodictyol, and enzyme inhibitory activities in ethyl acetate, methanol and water extracts. The methanol extract showed the highest antioxidant activity almost in all assays (excluding ferrous ion chelating activity). Moreover, the concentrations of the determined phytochemicals mainly chlorogenic acid content (21.4 +/- 0.7 mg/g), hesperidin (2.10 +/- 0.05 mg/g) and protocatechuic acid (0.94 +/- 0.02 mg/g) were the greatest for this extract. It was also the richest in all determined compounds - 21 phytochemicals were identified and water extract was the second most abundant in analytes (20 phytochemicals). The predominant compounds in water extract were the following: chlorogenic acid (20.3 +/- 0.12 mg/g), protocatechuic acid (0.83 +/- 0.02 mg/g) and caffeic acid (0.72 +/- 0.01 mg/g). Protocatechuic acid was prevailing in ethyl acetate extract (0.59 +/- 0.01 mg/g). In addition, in this extract the highest inhibitory activity of aamylase was found and the highest amount of verbascoside was determined in comparison to other extracts (0.53 +/- 0.01 mg/g). The tyrosinase inhibitory effect especially of methanol extract was also shown. Pearson correlation confirmed that the strong biological activity of methanol extract from V. dioscoridis resulted mainly from its high content of chlorogenic acid.
Onosma heterophyllum: Phenolic composition, enzyme inhibitory and antioxidant activities
Ozer, MS; Kirkan, B; Sarikurkcu, C; Cengiz, M; Ceylan, O; Atilgan, N; Tepe, B
Onosma species have frequently been used for their colouring and dyeing properties in foods and medicinal preparations. The antioxidant and enzyme inhibitory activities of the ethyl acetate, methanol and water extracts of Onosma heterophyllum (Griseb.) are described. Phytochemical compositions of these extracts were also determined. The water extract showed not only remarkable antioxidant activity in all assays but also considerable inhibitory activity on tyrosinase and a-glucosidase (112.44 mu mol KAEs/g dry plant and 984.36 mu mol ACEs/g dry plant, respectively). The methanol extract exhibited the highest inhibitory activity on acetylcholinesterase (AChE) and cc-amylase (79.18 mu mol GALAEs/g dry plant and 10.42 mu mol ACEs/g dry plant, respectively). Chromatographic analyses revealed that the water extract was found to be rich in phenolic and flavonoid contents. On the basis of the correlation coefficients calculated separately for all experimental parameter pairs, protocatechuic acid, p-hydroxybenzoic acid, syringic acid, p-coumaric acid and luteolin were found to be highly in correlation with the antioxidant and enzyme inhibitory activities. This study demonstrates that O. heterophyllum, contained in food preparations with various purposes for many years, could be used for the treatment of diabetes as well as its skin whitening effect.
Essential oil composition and antioxidant activity of Thymus longicaulis C. Presl subsp longicaulis var. longicaulis
Sarikurkcu, C; Ozer, MS; Eskici, M; Tepe, B; Can, S; Mete, E
This study is designed to examine the chemical composition and in vitro antioxidant activity of the hydro-distillated essential oil and various extracts obtained from Thymus longicaulis subsp. longicaulis var. Iongicaulis. GC and GC MS analysis of the essential oil were resulted in determination 22 different compounds, representing 99.61% of total oil. gamma-terpinene, thymol and p-cymene were determined as the major compounds of the oil (27.80, 27.65 and 19.38%. respectively). Antioxidant activities of the samples were determined by four different test systems namely beta-carotene/linoleic acid, DPPH, reducing power and chelating effect. Essential oil showed the highest antioxidant activity in beta-carotene/linoleic acid system among the experiments examined. In the case of other test systems, in general, methanol and water extracts exhibited the strongest activity profiles. Especially, reducing power of water extract was found superior than those of synthetic antioxidants. As well as the antioxidant activities of the extracts, they were evaluated in terms of their total phenolic and flavonoid contents. Hexane and water extracts were found to be rich-in phenolics. However, flavonoids were determined in the highest level in methanol extract. (C) 2010 Elsevier Ltd. All rights reserved.
Calamintha incana: Essential oil composition and biological activity
Popovic-Djordjevic, J; Cengiz, M; Ozer, MS; Sarikurkcu, C
The chemical composition of the essential oils (EO) isolated aerial parts of Calamintha incana (Sm.) Boiss. from Turkey was characterized by GC-FID and GC-MS analysis. The oxygenated monoterpenes trans-piperitone oxide (41.37%), piperitenone oxide (34.47%), piperitenone (6.67%), and monoterpene phenol thymol (3.37%) were found to be the major constituents of the essential oils of C. incana. The results of the antioxidant activity in phosphomolybdenum, radical scavenging (DPPH and ABTS) and reducing power activity (CUPRAC and FRAP) as well as metal chelating effects (ferrous ion chelating) showed that EO was the most potent in ABTS (129.58 +/- 2.21 mg TEs/g oil) and CUPRAC and FRAP (51.14 +/- 0.05 and 53.63 +/- 0.10 mg TEs/g, respectively) assays. In enzymes inhibitory activity assays of EO, the best result was achieved for tyrosinase (2.10 +/- 0.30 mg KAEs/g oil). The results suggest that EO might be considered as a potential source of bioactive agents to be used in food and pharmacological industries.
Determination of pKa Values of Clinically Important Perfluorochemicals in Nonaqueous Media
Hakli, O; Ertekin, K; Ozer, MS; Aycan, S
Perfluorochemicals (PFCs) are clinically and biotechnologically important species. In this work, the potentiometric titration method in nonaqueous media is proposed for the determination of acidity constant values of six different perfluorinated compounds. The saturated and nonsaturated perfluoroacids, perfluorocarnosine, and perfluoroalkyl-beta-alanine were potentiometrically titrated in acetonitrile, N,N-dimethylfornamide, acetone, ethanol, methanol, and pyridine with tetrabutylammonium hydroxide. The half-neutralization potentials and acidity constants of PFCs have been calculated from the titration curves by using the computerized derivative method. Except for R-F-Carnosine, all of the potentiometric fitration curves of the PFCs exhibited one stoichiometric and well-defined endpoints in all of the solvents employed. The reproducibility and sensitivity of the method were evaluated.
Datura innoxia and Dipsacus laciniatus: Biological activity and phenolic composition
Benabderrahim, MA; Sarikurkcu, C; Elfalleh, W; Ozer, MS
In the present study, we investigated the phenolic composition of the methanol extract from the aboveground of two plants of Turkish flora; Datura innoxia and Dipsacus laciniatus. In addition, the antioxidant (phosphomolybdenum, CUPRAC, FRAP, DPPH, ABTS) and enzyme inhibitory activities (a-amylase and tyrosinase), and total phenolic and flavonoids contents were determined in these plant extracts. The results showed high significant differences among plants (p <= 0.05) for the antioxidant capacities measured with the different methods, the enzyme inhibitory activities, and the all identified phenolic compounds except for hyperoside. From a total of 23 identified phenolic compounds, 19 were found in both plants. The main flavonoids identified were (+)-catechin and (-)-epicatechin and hyperoside. (+)-Catechin and (-)-epicatechin showed very high concentrations in D. innoxia (12937.39 +/- 108.86 and 24147.64 +/- 2512.35 mu g/g of dry plant, respectively) and in D. laciniatus (4947.99 +/- 14.18 and 13171.30 +/- 2410.76 mu g/g of dry plant, respectively). Hyperoside had interesting contents with comparable values (115.11 +/- 16.20 in D. innoxia, and 110.77 +/- 16.32 mu g/g of dry plant in D. laciniatus). The major phenolic acid was chlorogenic acid especially in D. laciniatus (12124.22 +/- 598.13 mu g/g of dry plant). These compounds were correlated to the high antioxidant and enzyme inhibitory activities for both species and confirm their medicinal traditional uses. Hence, further screening of bioactive compounds in different organs such as flowers and roots of these plants is crucial for the discovering of new source of natural antioxidants and enzyme inhibitors.
Phenolic profile, antioxidant and enzyme inhibitory potential of Onosma tauricum var. tauricum
Kirkan, B; Sarikurkcu, C; Ozer, MS; Cengiz, M; Atilgan, N; Ceylan, O; Tepe, B
This study focused on phytochemical characterization and biological evaluation, both antioxidant and enzymatic inhibition, of ethyl acetate (OTT-EtOAc), methanol (OTT-MeOH), and water (OTT-W) extracts from Onosma tauricum var. tauricum aerial parts. The OTT-W extract was found to be the richest one in terms of its phenolic and flavonoid contents. Amounts of the phenolic and flavonoid compounds were determined as 40.88 mu mol GAEs/g and 7.40 mu mol REs/g dry plant (dp), respectively. The OTT-MeOH and OTT-W extracts were also found to contain significant amounts of chlorogenic acid, rosmarinic acid, p-hydroxybenzoic acid, caffeic acid, rutin, p-coumaric acid, trans-cinnamic acid, luteolin, and apigenin. The OTT-W extract showed remarkable antioxidant activity in phosphomolybdenum, ferrous ion chelating, reducing power, and radical scavenging assays. The OTT-MeOH extract exhibited inhibitory activity on acetylcholinesterase (AChE) (54.62 mu mol GALAEs/g dp), while the extracts remained almost inactive on butyrylcholinesterase (BChE). The OTT-W extract also exhibited inhibitory activity on tyrosinase (90.66 mu mol KAEs/g dp) and alpha-glucosidase (2258.87 mu mol ACEs/g dp). The results suggest that especially the OTT-MeOH and OTT-W extracts can be used as a source of alternative natural products in the treatment of diseases caused by the enzymes evaluated here.
Enzyme and Biological Activities of the Water Extracts from the Plants Aesculus hippocastanum, Olea europaea and Hypericum perforatum That Are Used as Folk Remedies in Turkey
Sarikurkcu, C; Locatelli, M; Tartaglia, A; Ferrone, V; Juszczak, AM; Ozer, MS; Tepe, B; Tomczyk, M
Phenolic compounds are secondary metabolites that are found ubiquitously in plants, fruits, and vegetables. Many studies have shown that regular consumption of these compounds could have a positive effect on our health. The aim of this study was to compare the phytochemical contents of the water extracts from three different plants used as folk remedies in Turkey: Aesculus hippocastanum, Olea europaea, and Hypericum perforatum. A liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) analysis was performed to explore the phenolic profiles. The biological activities of these extracts were also evaluated in terms of their antioxidant activities (2,2-diphenyl-1-picrylhydrazyl DPPH, 2,2 '-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid ABTS, Ferric Reducing Antioxidant Power Assay FRAP, cupric ion reducing antioxidant capacity CUPRAC, beta-carotene, phosphomolybdenum, and metal chelating) and enzyme inhibitory properties (against acetylcholinesterase, butyrylcholinesterase, and tyrosinase). The aqueous extract of H. perforatum showed the highest levels of total phenolic, flavonoid, and saponin contents. Protocatechuic acid, vanillic acid, verbascoside, hesperidin, hyperoside, apigenin 7-hexosides, and quercetin were the most common compounds found in this species. The results confirm that A. hippocastanum, O. europaea, and H. perforatum represent a potential source of natural-derived molecules with positive properties that could be used as valid starting point for new food supplements, and drugs in the pharmaceutical, cosmetic, and food industries.
LC-MS/MS profiles and interrelationships between the enzyme inhibition activity, total phenolic content and antioxidant potential of Micromeria nervosa extracts
Sarikurkcu, C; Andrade, JC; Ozer, MS; Silva, JMFD; Ceylan, O; de Sousa, EO; Coutinho, HDM
The objective of this study was to quantify the phenolic compounds and to evaluate and compare the biological activities of the ethyl acetate (EtOAc), methanolic (MeOH) and aqueous extracts from the Micromeria nervosa aerial parts, based on their antioxidant activity and enzymatic inhibition. Total phenolic and flavonoid contents were calculated and individual compounds were detected using LC-ESI-MS/MS. The antioxidant activity was determined using six different assays while enzymatic activity was determined by alpha-amylase and tyrosinase enzyme inhibition. The main phenolic constituents detected in the extracts were rosmarinic acid. In the antioxidant assays the aqueous extract was shown to be more efficient than the others. The EtOAc and MeOH extracts presented higher inhibitory activity with respect to alpha-amylase and tyrosinase. Regardless of the solvent, the results suggest M. nervosa aerial extracts present a biological potential due to their antioxidant activity and enzymatic inhibition.
GC/MS Evaluation and In Vitro Antioxidant Activity of Essential Oil and Solvent Extracts of an Endemic Plant Used as Folk Remedy in Turkey: Phlomis bourgaei Boiss.
Sarikurkcu, C; Ozer, MS; Cakir, A; Eskici, M; Mete, E
This study was outlined to examine the chemical composition of hydrodistilled essential oil and in vitro antioxidant potentials of the essential oil and different solvent extracts of endemic Phlomis bourgaei Boiss. used as folk remedy in Turkey. The chemical composition of the oil was analyzed by GC and GC-MS, and the predominant components in the oil were found to be beta-caryophyllene (37.37%), (Z)-beta-farnesene (15.88%), and germacrene D (10.97%). Antioxidant potentials of the solvent extracts and the oil were determined by four testing systems including. beta-carotene/linoleic acid, DPPH, reducing power, and chelating effect. In. beta-carotene/linoleic acid assay, all extracts showed the inhibition of more than 50% at all concentrations. In DPPH, chelating effect, and reducing power test systems, the water extract with 88.68%, 77.45%, and 1.857 (absorbance at 700 nm), respectively, exhibited more excellent activity potential than other extracts (hexane, ethyl acetate and methanol) and the essential oil at 1.0 mg/mL concentration. The amount of the total phenolics and flavonoids was the highest in this extract (139.50 +/- 3.98. mu g gallic acid equivalents (GAEs)/mg extract and 22.71 +/- 0.05 mu g quercetin equivalents (QEs)/mg extract).

Browsing by Author "Ozer, MS"

Results Per Page

Sort Options