Browsing by Author "Ozkale E."

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    Indoor airborne fungal pollution in newborn units in Turkey
    (Springer International Publishing, 2017) Demirel R.; Sen B.; Kadaifciler D.; Yoltas A.; Okten S.; Ozkale E.; Berikten D.; Samson R.A.; Haliki Uztan A.; Yilmaz N.; Abaci Gunyar O.; Aydogdu H.; Asan A.; Kivanc M.; Ozdil S.; Sakartepe E.
    Pathogenic and/or opportunistic fungal species are major causes of nosocomial infections, especially in controlled environments where immunocompromised patients are hospitalized. Indoor fungal contamination in hospital air is associated with a wide range of adverse health effects. Regular determination of fungal spore counts in controlled hospital environments may help reduce the risk of fungal infections. Because infants have inchoate immune systems, they are given immunocompromised patient status. The aim of the present study was to evaluate culturable airborne fungi in the air of hospital newborn units in the Thrace, Marmara, Aegean, and Central Anatolia regions of Turkey. A total of 108 air samples were collected seasonally from newborn units in July 2012, October 2012, January 2013, and April 2013 by using an air sampler and dichloran 18% glycerol agar (DG18) as isolation media. We obtained 2593 fungal colonies comprising 370 fungal isolates representing 109 species of 28 genera, which were identified through multi-loci gene sequencing. Penicillium, Aspergillus, Cladosporium, Talaromyces, and Alternaria were the most abundant genera identified (35.14, 25.40, 17.57, 2.70, and 6.22% of the total, respectively). © 2017, Springer International Publishing AG.
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    Anti-acetylcholinesterase, antiprotozoal and cytotoxic activities of some Turkish marine algae
    (Parlar Scientific Publications, 2019) Cinar E.; Taskin E.; Tasdemir D.; Ozkale E.; Grienke U.; Firsova D.
    The crude (MeOH:CHCl3) and n-hexane-soluble extracts from four brown algae (Phaeophyceae) [Petalonia fascia, Cystoseira crinita, Cystoseira foeniculacea, and Halopteris scoparia], one red alga (Rhodophyta) [Jania rubens] and three green algae (Chlorophyta) [Chaetomorpha aerea, Codium fragile subsp. fragile, and Ulva compressa] from Turkish coasts (Izmir Bay, Ayvalik and Çanakkale) were assessed in vitro for their acetylcholinesterase (AChE) inhibitory activities at 200, 150, 50, 20 μg/mL test concentration with Ellman's method. The crude extract of P. fascia possessed the highest inhibition (IC5 0 value of 19,22±10,47 μg/mL) against AChE. Galanthamine HBr was used as standard drug that gave against AChE enzyme IC5 0 of 3.44±1.14 μM. In the second stage, the crude, hexane-soluble, chloroform-soluble and water-methanol soluble extracts of the marine algae were observed in vitro against parasitic protozoa (Plasmodium falciparum, Trypanosoma brucei brucei, Trypanosoma cruzi ve Leishmania infantum). According to results, the most potent protozoal activities were shown by the Khex of C. crinita (IC5 0 value of 10,62 μg/ml), followed by the Khex of C. fragile subsp. fragile (IC50 value of 11,89 μg/ml). The hekzan-soluble (Khex) and chloroform-soluble (KCH) extracts gave the best results. The marine algae were also tested on MRC-5 cells (human fibroblasts) for by controlling tamoxifen. The extracts of H. scoparia, C. aerea ve C. fragile subsp. fragile showed toxicity. © by PSP
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    Determination of antifungal efficiency of some fungicides and secondary metabolites of Trichoderma species against Botrytis cinerea
    (Triveni Enterprises, 2021) Savas N.G.; Yildiz M.; Eltem R.; Ozkale E.
    Aim: The present study aimed to investigate the antifungal effects of fenhexamid, cyprodinil+fludioxanil, Bacillus subtilis QST 713, Trichoderma secondary metabolites (EGE-K-38 and EGE-K-71) on 6 isolates of necrotrophic fungi (Botrytis cinerea) under in- vitro conditions. Methodology: A dual culture technique was used to evaluate the effects of volatile compounds produced by Trichoderma in culture media against Botrytis cinerea isolates. For non-volatile and volatile metabolites, measurements for growth inhibition were made with the mixture of filter sterilized liquid medium of Trichoderma and potato dextrose agar. The fungicidal effects of these non-volatile metabolites and fungicides were first evaluated according to the effective concentration (EC ) data of 50 growing mycelium inhibition of disease agents. Results: According to the results obtained, the drug/agent with the lowest fungicidal activity was determined as fenhexamide, which seems to have the lowest EC value of 0.05 ug m l-1. Both 50 non-volatile metabolites and volatile metabolites of Trichoderma strains showed strong inhibition against B. cinerea isolates under experimental conditions. The highest growth inhibition percentage was determined with volatile metabolites of Trihoderma atroviride EGE-K-71 strain as 71.8%. Interpretation: The study suggest that filtrates that contain secondary metabolites and volatile compounds of Trichoderma strains produced in both liquid culture and dual culture conditions can be used as an effective fungal control agents against pre and postharvest contamination of grapes with Botrytis cinerea. © 2021 Triveni Enterprises. All rights reserved.