Browsing by Author "Ozkul, Y"

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    Effects of oral intake of cetirizine HCl and desloratadine molecules on the middle ear mucosa: an experimental animal study
    Songu, M; Ozkul, Y; Kirtay, S; Arslanoglu, S; Ozkut, M; Inan, S; Onal, K
    We have planned to demonstrate histopathologic effects of mid- or long-term oral use of desloratadine and cetirizine HCl molecules on middle ear mucosa of rats. Thirty-six rats were randomized equally into six groups. Desloratadine groups received once daily doses of 1 mg/ml desloratadine for 30 (D30 Group) or 60 (D60 Group) days. The Cetirizine study groups were given once daily doses of 1 mg/ml cetirizine for 30 (S30 Group) or 60 (S60 Group) days. Control groups were given 2 cc physiologic saline using orogastric gavage method through a 12 G gavage catheter for 30 (K30 Group) or 60 (K60) days. At the end of 30 days, D30, S30 and K30 Groups were sacrificed. Tissue samples harvested from groups were evaluated between 1 and 4 Grades for histological characteristics of middle ear canal, eardrum, middle ear epithelium and connective tissue, edema, vascular congestion and inflammatory cells. In the control group no pathological finding was encountered in rats sacrificed on 30 and 60 days. No statistical difference was observed when groups were compared on external ear epithelial tissue, external ear sebaceous gland, middle ear inflammation, and middle ear capillary dilatation both on 30 and 60 days. Tympanic membrane collagen was more evident in D30 and D60 groups when compared with C30 and C60 groups. Comparison of histopathological grading results between 30 and 60 days revealed no significant changes. In conclusion, oral intake of cetirizine and desloratadine preparations has effects of tympanic membrane collagen, degrees of edema and vascular congestion being more prominent with desloratadine molecule.
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    Efficiency of Bioflavonoids in the Prevention of Experimental Myringosclerosis
    Ilknur, AE; Dundar, R; Basoglu, S; Inan, S; Aktas, S; Aslan, H; Ozkul, Y; Ozturkcan, S; Katilmis, H
    Objectives/Hypothesis: It has been noted that some materials with anti-inflammatory and antioxidant effects decrease sclerotic lesions in experimental myringosclerosis. Our purpose in this study is to investigate the effect of micronized purified flavonoid fraction (MPFF), an antioxidant with anti-inflammatory effects, in experimental myringosclerosis in guinea pigs. Materials and Methods: Two study groups were formed. The first group was administered 100mg/kg/day MPFF by catheter for five days before myringotomy and 10 days after myringotomy, while the second group was administered distilled water by the same method, before and after myringotomy. On the 15th day of the study, after the tympanic membranes were examined otomicroscopically for myringosclerosis, they were removed by dissection together with the bone annulus, for histochemical and immunohistochemical examinations. Results: In the MPFF group, the otomicroscopical sclerosis score, inflammation score and tympanic membrane thickness were significantly less than those in the untreated group (p<0.05). It was also determined that the immunoactivity of the anti-VEGF, anti-TGF-beta, anti-eNOS, anti-iNOS, and anti-IL1-beta primary antibodies, which are known to have an important role in angiogenesis and inflammation, significantly decreased in the MPFF group (p<0.05). Conclusion: In conclusion, this study shows that orally administered MPFF can be efficient in the prevention of experimental myringosclerosis in guinea pigs.