Browsing by Author "Ozturk S."
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Item Comparative in vitro cytotoxic effects of ornidazole, metronidazole and ciprofloxacin against Trichomonas vaginalis trophozoites(E.S.I.F.T. srl, 2004) Inceboz T.; Inceboz U.; Ozturk S.A strain of Trichomonas vaginalis, isolated from a patient complaining of vaginal discharge, was incubated and cultivated to compare the in vitro effects of ornidazole, metronidazole and ciprofloxacin on T. vaginalis trophozoites in terms of minimal inhibition concentrations (MICs) and minimal lethal concentrations (MLCs). MIC levels at 24 hours for ornidazole, metronidazole and ciprofloxacin were 50 mg/mL, 50 mg/mL, and 750 mg/mL. Corresponding MLC levels were the same. In this in vitro study, ornidazole was found to be the most effective drug among 3 drugs tested against T. vaginalis trophozoites in terms of MIC and MLC levels. It is interesting to note that ciprofloxacin, although not as effective as the others, also had some cytotoxic effect on T. vaginalis trophozoites.Item Cutting-tool recycling process with the zinc-melt method for obtaining thermal-spray feedstock powder (WC-Co)(2013) Altuncu E.; Ustel F.; Turk A.; Ozturk S.; Erdogan G.Various recycling processes for WC-Co cermets from cutting tools, such as chemical modification, thermal modification, the cold-stream method and the electrochemical method have been investigated and some of them are actually employed in industry. However, these conventional methods have many problems to be solved and they are not always established technologies. Therefore, a more economical and high-efficiency recycling procedure needs to be developed. In this study we investigated the applicability of the zinc-melt method (ZMM) for recycling WC-Co as a powder from cutting-tool scraps. It was proven that ZMM is an available technique for recovering the WC powder from the cutting tools. WC-Co powders are recovered and then spray dried, sintered and obtained as a feedstock material for thermal-spray coating processes.Item Effect of olive mill waste addition on the properties of porous fired clay bricks using Taguchi method(Academic Press, 2016) Sutcu M.; Ozturk S.; Yalamac E.; Gencel O.Production of porous clay bricks lightened by adding olive mill waste as a pore making additive was investigated. Factors influencing the brick manufacturing process were analyzed by an experimental design, Taguchi method, to find out the most favorable conditions for the production of bricks. The optimum process conditions for brick preparation were investigated by studying the effects of mixture ratios (0, 5 and 10 wt%) and firing temperatures (850, 950 and 1050 °C) on the physical, thermal and mechanical properties of the bricks. Apparent density, bulk density, apparent porosity, water absorption, compressive strength, thermal conductivity, microstructure and crystalline phase formations of the fired brick samples were measured. It was found that the use of 10% waste addition reduced the bulk density of the samples up to 1.45 g/cm3. As the porosities increased from 30.8 to 47.0%, the compressive strengths decreased from 36.9 to 10.26 MPa at firing temperature of 950 °C. The thermal conductivities of samples fired at the same temperature showed a decrease of 31% from 0.638 to 0.436 W/mK, which is hopeful for heat insulation in the buildings. Increasing of the firing temperature also affected their mechanical and physical properties. This study showed that the olive mill waste could be used as a pore maker in brick production. © 2016 Elsevier LtdItem Synergistic role of three dimensional niche and hypoxia on conservation of cancer stem cell phenotype(Elsevier B.V., 2016) Gorgun C.; Ozturk S.; Gokalp S.; Vatansever S.; Gurhan S.I.D.; Urkmez A.S.Hypoxia is a pathalogical condition in which tissues are deprived of adequate oxygen supply. The hypoxia effect on tumors has a critically important role on maintenance of cancer stem cell phenotype. The aim of this study is to investigate the effects of hypoxia on cancer stem cells on three dimensional (3D) in vitro culture models. Osteosarcoma stem cells characterized by CD133 surface protein were isolated from osteosarcoma cell line (SaOS-2) by magnetic-activated cell sorting (MACS) technique. Isolated CD133+ and CD133− cells were cultivated under hypoxic (1% O2) and normoxic conditions (21% O2) for 3 days. For the 3D model, bacterial cellulose scaffold was used as the culture substrate. 3D morphologies of cells were examined by scanning electron microscopy (SEM); RT-PCR and immunocytochemistry staining were used to demonstrate conservation of the cancer stem cell phenotype in 3D environment under hypoxic conditions. Cell viability was shown by MTT assay on 3. and 7. culture days. This study is seen as an introduction to develop a 3D hypoxic cancer stem cell based tumor model to study CSC behavior and tumor genesis in vitro. © 2016Item The effect of electro magnetic fields on cell behaviour; [Elektro Manyetik Alanlarin Hücre Davranisina Etkisi](Institute of Electrical and Electronics Engineers Inc., 2017) Tuglu M.I.; Gulbagca F.; Sal D.H.; Saygili S.; Sonmez P.K.; Aydemir I.; Ozkut M.; Ozturk S.; Gumusay M.; Kaya A.; Keskin N.The biologic effects of electomagnetic fields application on medical field has been increased. Their effect on medical treatment depends on cell behaviour which can be observed by in vivo and in vitro studies. Their effect on the stem cells and the cell lines and the organs of experimental animals with morphological alterations are helpful to understand cell behaviour. The behaviour of the cells can be analyse at ultrastructural level by scaning electron microscopy. Application of electomagnetic fields cause increase of proliferation, migration and improvement of wound healing with beneficial effects while they cause oxidative stress and cell death including apoptosis for cancer cells in different types of cell line. The minimal side effect with maximum beneficial treatment of these products suggest that they could be very useful for clinical trials. © 2016 IEEE.Item Effects of electromagnetic fields application on experimental varicocele model in rats; [Sicanlarda Deneysel Varikosel Modelinde Elektromanyetik Alan Uygulamalarinin Etki](Institute of Electrical and Electronics Engineers Inc., 2017) Gulbagca F.; Sal D.H.; Saygili S.; Sonmez P.K.; Aydemir I.; Ozkut M.; Ozturk S.; Gumusay M.; Kaya A.; Tuglu M.I.Varicocele is the most clinical condition which can be treated by surgery. The frequency in male is 20%. The half of infertile patient has this pathology which there is dilatation in plexus pampiniformis veins of testes. Varicocele causes increase of heat or increse of oxidative stres in seminifer tubules where they produce pathology for spermatogenesis. Low frequency magnetic fields are useful for the treatment of many area where they become important role. In this study the effect of low frequency magnetic fields on the experimental varicocele model of rat testes was investigated. The parameters for oxidative stres suggest eNOS and iNOS with apoptosis were evaluated by immunohistochemical staining. There was increaese in eNOS, iNOS and apoptosis by the varicocele experiment where there was testicular damage and low frequency magnetic fields caused decrease in oxidative stress and apoptosis. Application of magnetic fields can help the clinical varicocele pathologies either with treatment or supportive effect. © 2016 IEEE.Item Prediction of nano etching parameters of silicon wafer for a better energy absorption with the aid of an artificial neural network(Elsevier B.V., 2018) Kayabasi E.; Ozturk S.; Celik E.; Kurt H.; Arcaklioğlu E.To enhance energy absorption of photovoltaics, several etching experiments with various parameters were performed. In addition, an Artificial Neural Network (ANN) simulation was utilized to predict chemical nano etching parameters such as masking and etching durations for Silicon (Si) solar cell applications to reach minimum surface reflectance in an optimum etching duration. Experiments were performed with different masking and etching durations to determine the characteristics of surface reflectance of micro textured n-type single crystalline Si wafers in 25mmx25mm width and 300 µm thickness to provide training data for ANN. For this purpose, solutions with identic properties including Ag nanoparticles were applied with different application durations on the surfaces of n-type single crystalline Si wafers to cover partially the Si surfaces with Ag nano-particles at masking step. After, partially masked Si surfaces were exposed to chemical nano etching to develop nano-sized porous structures under different etching durations in an identic acidic etching solution. For the etching of Si wafers, 32 masking and etching processes were performed. Reflectance measurements and SEM images were evaluated to determine the optimum etching duration resulting the best surface quality with minimum reflectance. In addition, reflectance values were utilized as input data for training, testing and validation steps of developed ANN. In the ANN simulation, 70% of reflectance values were used as training, 15% of reflectance values were used as validation and 15% of reflectance values were used to test data in the ANN. Consequently, surface reflectance values under different masking and etching durations were predicted with the new parameter set by using the trained ANN with a success level above 99%. © 2018 Elsevier B.V.Item A database for screening and registering late onset Pompe disease in Turkey(Elsevier Ltd, 2018) Gokyigit M.C.; Ekmekci H.; Durmus H.; Karlı N.; Koseoglu E.; Aysal F.; Kotan D.; Ali A.; Koytak P.K.; Karasoy H.; Yaman A.; Sengun İ.S.; Sayin R.; Tiftikcioglu B.I.; Soysal A.; Tutkavul K.; Bayrak A.O.; Kısabay A.; Elci M.A.; Yayla V.; Yılmaz İ.A.; Ozdamar S.E.; Erdogan C.; Tasdemir N.; Serdaroglu Oflazer P.; Deymeer F.; Parman Y.; Kendirci M.; Sayan S.; Celebi L.G.; Uluç K.; Tanrıdağ T.; Yuceyar N.; Ekmekci O.; Colakoglu B.D.; Ozturk S.; Tireli H.; Selcuki D.; Neyal A.M.; Kayran Y.; Aluclu M.U.; Koyuncuoglu H.R.; Tokucoglu F.; Secil Y.; Guney F.; Gozke E.; Balaban H.; Akalın M.A.; Koc A.F.; Mulayim S.; Turgut N.The aim of this study was to search for the frequency of late onset Pompe disease (LOPD) among patients who had a myopathy with unknown diagnosis registered in the pre-diagnostic part of a novel registry for LOPD within a collaborative study of neurologists working throughout Turkey. Included in the study were 350 patients older than 18 years who have a myopathic syndrome without a proven diagnosis by serum creatine kinase (CK) levels, electrodiagnostic studies, and/or muscle pathology, and/or genetic tests for myopathies other than LOPD. Acid alpha glucosidase (GAA) in dried blood spot was measured in each patient at two different university laboratories. LOPD was confirmed by mutation analysis in patients with decreased GAA levels from either both or one of the laboratories. Pre-diagnostic data, recorded by 45 investigators from 32 centers on 350 patients revealed low GAA levels in a total of 21 patients; from both laboratories in 6 and from either one of the laboratories in 15. Among them, genetic testing proved LOPD in 3 of 6 patients and 1 of 15 patients with decreased GAA levels from both or one of the laboratories respectively. Registry was transferred to Turkish Neurological Association after completion of the study for possible future use and development. Our collaborative study enabled collection of a considerable amount of data on the registry in a short time. GAA levels by dried blood spot even from two different laboratories in the same patient may not prove LOPD. LOPD seemed to be rarer in Turkey than in Europe. © 2017 Elsevier B.V.Item The effect of personal, familial, and environmental characteristics on acne vulgaris: A prospective, multicenter, case controlled study(Edizioni Minerva Medica, 2019) Karadag A.S.; Balta I.; Saricaoglu H.; Kiliç S.; Kelekçi K.H.; Yildirim M.; Arica D.A.; Ozturk S.; Karaman G.; Çerman A.A.; Bilgili S.G.; Turan E.; Demirci M.M.; Uzunçakmak T.K.; Guvenç S.C.; Ataseven A.; Ferahbaş A.; Aksoy B.; Çolgeçen E.; Ekiz O.; Demir F.T.; Bilgiç O.; Çakmak S.; Uçmak D.; Ozuguz P.; Konkuralp Y.K.; Ermertcan A.T.; Gokdemir G.; Başkan E.B.; Alyamaç G.; Şanli H.BACKGROUND: There are only a few studies about epidemiological features of acne vulgaris in the literature. The aim of this study was to analyze demographic, clinical, familial and environmental characteristics of acne, the role of diet and aggravating factors and association of these factors with acne severity. METHODS: Patients with a diagnosis of mild-moderate to severe acne were consecutively interviewed at the participating centers during the study period. RESULTS: A total of 3826 patients and 759 control patients were involved in this study. Mild acne was the most common type of acne, and most of the lesions were localized on face followed by the trunk. The severity of acne was worse in patients who had a positive family history of acne. The most common triggering factor was psychological stress. We found a positive correlation with chocolate, bread, green tea, milk, white sugar, ripe banana, ice cream, apple, orange, and red meat consumption. As we compare the acne severity according to geographical features we detected mild- moderate acne was more common in Mediterranean region and severe acne was more common in East Anatolian region. Family history positivity was more common in Aegean region and least common in Central Anatolian region. There was statistically significant relationship as we compare acne severity and dietary factors such as chocolate, dairy products such as milk, sunflower seed consumption within the geographical regions. CONCLUSIONS: This study presents the demographic and clinical characteristics of acne patients in Asian and the European parts of Turkey. We believe that this study will provide a useful overview of acne in Turkey. © 2017 EDIZIONI MINERVA MEDICA.Item Influence of tea waste concentration in the physical, mechanical and thermal properties of brick clay mixtures(Elsevier Ltd, 2019) Ozturk S.; Sutcu M.; Erdogmus E.; Gencel O.The sustainability of raw materials used in construction industry consumed large quantities of material has great importance. The use of pore-makers in the brick production is reducing the consumption of clean clay resources as well as lightness of the baked brick body. Many organic or inorganic additives have been used as pore-making in brick production for improving thermal performance. In this study, tea waste (TW) were used at different concentrations in the brick clay mixtures to examine its effects on baked brick properties. In addition to micro-structure investigations, physical, mechanical and thermal properties of bricks produced were investigated. It is concluded that tea waste additive up to 10% in brick body can be used for structural application and isolation while ratios more 10% tea waste additive for only isolation purposes. Tea wastes can be used as a pore-making additive in the brick production. © 2019 Elsevier LtdItem Development and characterization of cancer stem cell-based tumoroids as an osteosarcoma model(John Wiley and Sons Inc., 2020) Ozturk S.; Gorgun C.; Gokalp S.; Vatansever S.; Sendemir A.Three-dimensional (3D) cancer tumor models are becoming vital approaches for high-throughput drug screening, drug targeting, development of novel theranostic systems, and personalized medicine. Yet, it is becoming more evident that the tumor progression and metastasis is fueled by a subpopulation of stem-like cells within the tumor that are also called cancer stem cells (CSCs). This study aimed to develop a tumoroid model using CSCs. For this purpose CD133+ cells were isolated from SaOS-2 osteosarcoma cell line with magnetic-activated cell sorting. To evaluate tumoroid formation ability, the cells were incubated in different cell numbers in agar gels produced by 3D Petri Dish® method. Subsequently, CD133+ cells and CD133− cells were co-cultured to investigate CD133+ cell localization in tumoroids. The characterization of tumoroids was performed using Live&Dead staining, immunohistochemistry, and quantitative polymerase chain reaction analysis. The results showed that, CD133+, CD133− and SaOS-2 cells were all able to form 3D tumoroids regardless of the initial cell number, but, while 72 hr were needed for CD133+ cells to self-assemble, 24 hr were enough for CD133− and SaOS-2 cells. CD133+ cells were located within tumoroids randomly with high cell viability. Finally, when compared to two-dimensional (2D) cultures, there were 5.88, 4.14, 6.95, and 1.68-fold higher messenger RNA expressions for Sox2, OCT3/4, Nanog, and Nestin, respectively, in CD133+ cells that were cultured within 3D tumoroids, showing longer maintenance of stem cell phenotype in 3D, that can allow more relevant screening and targeting efficiency in pharmaceutical testing. It was concluded that CSC-based tumoroids are propitious as 3D tumor models to fill the gap between conventional 2D in vitro culture and in vivo animal experiments for cancer research. © 2020 Wiley Periodicals LLCItem The Impact of the COVID-19 Lockdown on the Quality of Life in Chronic Neurological Diseases: The Results of a COVQoL-CND Study(S. Karger AG, 2021) Sahin S.; Karsidag S.; Cinar N.; Ates M.F.; Demir S.; Eren F.; Neyal A.; Kisabay Ak A.; Bora Tokcaer A.; Erkoc Ataoglu E.; Akkaya S.N.; Aciman Demirel E.; Koc F.; Ozturk S.; Ekmekyapar Firat Y.; Okluoglu T.; Togrol E.; Erdemoglu A.K.; Ergin N.; Sayin R.; Demir A.; Yilmaz S.E.Background: Coronavirus disease 2019 (COVID-19) pandemic and lockdown period may induce an impairment in quality of life (QoL), disruption in treatment (DIT), and posttraumatic stress disorder (PTSD) in chronic neurological diseases (CNDs). To reach this information, a multicenter, cross-sectional study (COVQoL-CND) was planned. Parkinson's disease (PD), headache (HA), multiple sclerosis (MS), epilepsy (EP), polyneuropathy (PNP), and cerebrovascular disease (CVD) were selected as the CND. Methods: The COVQoL-CND study includes demographic data, the World Health Organization Quality of Life short form (WHOQOL-BREF), and Impact of Event Scale-Revised (IES-R) forms. Results: The mean age of a total of 577 patients was 49 ± 17 (19-87 years), and the ratio of female/male was 352/225. The mean age of patients with PD, HA, MS, EP, PNP, and CVD were 65 ± 11, 39 ± 12, 38 ± 10, 47 ± 17, 61 ± 12, and 60 ± 15 years, respectively. The IES-R scores were found to be higher in the younger group, those with comorbid disease, contacted with CO-VID-19 patients, or diagnosed with COVID-19. In the group with a high IES-R score, the rate of DIT was found to be high. IES-R scores were negatively correlated with QoL. IES-R total scores were found highest in the CVD group and lowest in the PD group. The ratio of DIT was found highest in the PNP group and the lowest in the EP group. Contact with CO-VID-19 patients was high in the EP and HA group. Conclusions: The results of the COVQoL-CND study showed that lockdown causes posttraumatic stress and deterioration in the QoL in CND. © 2021Item Algal flora of an extremophile ecosystem: Kaklik Cave (Denizli, Turkey)(Maejo University, 2021) Ozturk S.Caves are specific ecosystems with both biotic and abiotic characteristics. Turkey has approximately 20,000 caves, and the biology of most of them has yet to be studied. Kaklik Cave is sinkhole-shaped and its entrance is quite large. A mineral-rich spring forms travertine as it enters the cave giving it unique characteristics. The study aims to determine the algal flora of Kaklik Cave. A total of eighty-six taxa were identified. Among them, twenty-one taxa were recorded for the first time as freshwater algal flora of Turkey. Also, the relationships among the most effective environmental parameters, the most frequently found algae taxa composition, and sampling sites were observed. © 2021 by Maejo University, San Sai, Chiang Mai, 50290 Thailand.Item Investigation of Stem Cell Applications on In Vitro Fertilization in Rats(Hellenic Veterinary Medical Society, 2021) Gumurdu A.; Ozturk S.; Aydemir I.; Tuglu M.I.We aimed to search the effects of bone marrow-derived mesenchymal stem cell-conditioned media on in vitro fertilization by investigation of lifetime of germ cells cleavage, degeneration rates and embryo quality. For this purpose, firstly MSCs were isolated from femurs and tibias of the rat, and cells were cultured until the fourth passage. Sperm and oocytes were collected from male and female rats. Oocytes were added in Human Tubal Fluid Media (HTFM), Single Step Media (SSM), Alpha-MEM Media (AMM) and Bone Marrow-Derived Mesenchymal Stem Cell-Conditioned Media (CM). Thousand sperm were added into the media which including oocytes. Embryos were allowed to produce by IVF. The development of the embryos was followed until the 11th day, and the arrest, degeneration rates and alive embryos were established. The embryos reached 2, 4, 8, 16 cells stages and morula stage in the CM. While AMM had a negative effect on fertilization and embryo development, the most favourable effect was shown to be caused by CM in comparison with the other medias. These results have shown that the beneficial effects of CM in IVF would be a significant increase in the rate of fertility and development of embryos © 2021 A GUMURDU, S OZTURK, I AYDEMIR, MI TUGLUItem Synergic effect of recycled paper sludge and expanded perlite on the engineering properties of porous clay bricks: A new mathematical modelling approach(Elsevier Ltd, 2023) Sutcu M.; Ozturk S.; Gencel O.Lightweight bricks produced with addition of different additive materials such as expanded perlite (EP) and recycling paper sludge (RPS) waste have been accomplished by a pug mill. Porous fired bricks were produced by using organic and inorganic additives such as paper waste and expanded perlite as pore-makers. In the experimental study, the seventeen mixture sets were prepared with Box-Behnken experimental design procedure of two additive ratios and firing temperatures. The brick samples were produced with shaping by extrusion method and firing at 850, 950 and 1050 °C for two hours after drying. Physical characteristics such as bulk density, apparent porosity and water absorption, and also, compressive strength, thermal conductivity and microstructural properties of brick samples were studied. Influences of process parameters on brick properties were investigated with a detailed neuro-regression analysis. In conclusion, the best brick composition (10 % EP + 10 %RPS by weight) in terms of thermal conductivity corresponding to acceptable mechanical properties was optimized. Accordingly, the compressive strength values of all samples are around 10 to 27 MPa. Also, the samples with high additive content (especially 10 %RPS + 5 %EP sample) exhibited the lowest thermal conductivity value (0.432 W/mK) due to the low firing temperature. © 2023 Elsevier LtdItem Optimization of thermal conductivity and lightweight properties of clay bricks(Elsevier B.V., 2023) Ozturk S.As the demand for energy increases worldwide, the construction industry, one of the most energy-intensive sectors, requires lightweight and high-thermal-performance materials. To address this, a multi-objective optimization approach was used in this study to identify the most suitable solutions for producing strength-fired clay bricks with low thermal conductivity. Porous bricks were produced with organic waste additives to illustrate the relationship between compressive strength and thermal conductivity. A 3-factor and 3-stage Box-Behnken experimental design was utilized, with a pore-forming additive ratio (0–10% by weight of pine nut shells), firing temperature (850–1050 °C), and firing time (2–6 h) as the variables. The fired bricks' physical, mechanical, and thermal properties were determined using standard analysis methods. The bricks' compressive strength and thermal conductivity functions were generated using neuro-regression systematics. Multiple targets were defined, including minimizing the thermal conductivity and maximizing the compressive strength of the bricks. The genetic algorithm was employed to identify Pareto-optimal solutions, and the final sets of low thermal conductive-strength brick production were chosen based on these solutions. Two sets were proposed to achieve the lowest thermal conductivity, and the results confirmed the validity and feasibility of the optimization study. © 2023 Karabuk UniversityItem Evaluation and follow-up of patients diagnosed with hypophysitis: A cohort study(Oxford University Press, 2024) Hacioglu A.; Karaca Z.; Uysal S.; Ozkaya H.M.; Kadioglu P.; Selcukbiricik O.S.; Gul N.; Yarman S.; Koksalan D.; Selek A.; Canturk Z.; Cetinarslan B.; Corapcioglu D.; Sahin M.; Sah Unal F.T.; Babayeva A.; Akturk M.; Ciftci S.; Piskinpasa H.; Dokmetas H.S.; Dokmetas M.; Sahin O.; Eraydln A.; Fenkci S.; Ozturk S.; Akarsu E.; Omma T.; Erkan B.; Burhan S.; Pehlivan Koroglu E.; Saygili F.; Kilic Kan E.; Atmaca A.; Elbuken G.; Alphan Uc Z.; Gorar S.; Hekimsoy Z.; Pekkolay Z.; Bostan H.; Bayram F.; Yorulmaz G.; Sener S.Y.; Turan K.; Celik O.; Dogruel H.; Ertorer E.; Turhan Iyidir O.; Topaloglu O.; Cansu G.B.; Unluhizarci K.; Kelestimur F.Objective: Primary hypophysitis might be challenging to diagnose, and there is a lack of evidence regarding optimal treatment strategies due to rarity of the disease. We aim to investigate the clinical features and compare the outcomes of different management strategies of primary hypophysitis in a large group of patients recruited on a nationwide basis. Design: A retrospective observational study. Methods: The demographic, clinical, and radiologic features and follow-up data were collected in study protocol templates and analyzed. Results: One hundred and thirteen patients (78.8% female, median age: 36 years) were included. Lymphocytic (46.7%) and granulomatous hypophysitis (35.6%) were the prevailing subtypes out of 45 patients diagnosed after pathologic investigations. Headache (75.8%) was the most common symptom, and central hypogonadism (49.5%) was the most common hormone insufficiency. Of the patients, 52.2% were clinically observed without interventions, 18.6% were started on glucocorticoid therapy, and 29.2% underwent surgery at presentation. Headache, suprasellar extension, and chiasmal compression were more common among glucocorticoid-treated patients than who were observed. Cox regression analysis revealed higher hormonal and radiologic improvement rates in the glucocorticoid-treated group than observation group (hazard ratio, 4.60; 95% CI, 1.62-12.84 and HR, 3.1; 95% CI, 1.40-6.68, respectively). The main indication for surgery was the inability to exclude a pituitary adenoma in the presence of compression symptoms, with a recurrence rate of 9%. Conclusion: The rate of spontaneous improvement might justify observation in mild cases. Glucocorticoids proved superior to observation in terms of hormonal and radiologic improvements. Surgery may not be curative and might be considered in indeterminate, treatment-resistant, or severe cases. © 2024 The Author(s). Published by Oxford University Press on behalf of European Society of Endocrinology. All rights reserved.Item Nationwide prospective audit for the evaluation of appendicitis risk prediction models in adults: Right iliac fossa treatment (RIFT) - Turkey(Oxford University Press, 2024) Yalcinkaya A.; Yalcinkaya A.; Balci B.; Keskin C.; Erkan I.; Yildiz A.; Kamer E.; Leventoglu S.; Caglikulekci M.; Zarbaliyev E.; Sevmis M.; Ulgen Y.; Altinel Y.; Meric S.; Akbas A.; Hacim N.A.; Vartanoglu Aktokmanyan T.; Aktimur Y.E.; Calikoglu F.; Gullu H.F.; Durma A.G.; Acar S.; Ciftci E.; Balik E.; Kulle C.B.; Ozata I.H.; Tufekci T.; Tatar C.; Sevinc M.M.; Sevik H.; Ertürk C.; Kiraz I.N.; Ozben V.; Aytac E.; Aliyeva Z.; Mutlu A.U.; Tanal M.; Celayir M.F.; Bozkurt E.; Yetkin S.G.; Ergin E.; Attaallah W.; Uprak T.K.; Omak A.; Simsek O.; Bozkurt M.A.; Kara Y.; Bozdag E.; Yirgin H.; Ozcan A.; Okkabaz N.; Ozdenkaya Y.; Haksal M.C.; Pekuz C.K.; Duru S.; Sivrikoz E.; Ozdemir Y.; Tan N.; Yarbug Karayali F.; Taghiyeva A.; Tirnova I.; Erenler Bayraktar I.; Bayraktar O.; Emsal E.Z.; Dalkilic M.I.; Yesiltas M.; Tok H.; Karakas D.O.; Pusane A.; Demirer A.I.; Sahin H.B.; Gok A.F.K.; Bozkurt H.A.; Yildirim M.; Uzunyolcu G.; Yanar H.T.; Ergun S.; Kutluk F.; Uludag S.S.; Zengin A.K.; Ozcelik M.F.; Sanli A.N.; Altuntas Y.E.; Memisoglu E.; Sari R.; Akdogan O.; Kucuk H.F.; Ozkan O.F.; Ulgur H.S.; Kirkan E.F.; Yuksekdag S.; Rencuzogullari A.; Aktas M.K.; Aba M.; Demirel A.O.; Eray I.C.; Aydogan B.; Cetinkunar S.; Yener K.; Sozutek A.; Irkorucu O.; Bayrak M.; Altintas Y.; Alabaz O.; Atasever A.; Erdogrul G.; Kupeli A.H.; Muhammedoglu B.; Kokdas S.; Kaya M.; Uysal E.; Yildirim A.C.; Zeren S.; Ekici M.F.; Algin M.C.; Kucuk G.O.; Eraslan H.; Aybar E.; Polat S.; Ceylan A.; Isik O.; Kural S.; Aktas A.; Bakar B.; Uzunoglu M.Y.; Gulcu B.; Ozturk E.; Devay A.O.; Taspinar E.; Balcin O.; Aksoy F.; Garip G.; Yalkin O.; Iflazoglu N.; Yigit D.; Kaya R.B.; Ugur M.; Kilic E.; Dedemoglu A.; Arslan R.E.; Temiz M.; Aydin C.; Demirli Atici S.; Kaya T.; Ozturk S.; Calik B.; Kilinc G.; Acar T.; Acar N.; Cengiz F.; Ureyen O.; Tan S.; Ilhan E.; Turk Y.; Durak A.T.; Yilmaz M.; Mercan M.; Atci R.; Sokmen S.; Bisgin T.; Egeli T.; Yildirim Y.; Safak T.; Celik K.; Yilmaz E.M.; Kirnap M.; Demirkiran A.E.; Sekerci U.U.; Karacan E.; Bilgic E.; Ozmen M.M.; Guldogan C.E.; Gundogdu E.; Moran M.; Erol T.; Dincer H.A.; Kirimtay B.; Yilmaz S.; Cennet O.; Yildiz A.; Sahin C.; Akyol C.; Koc M.A.; Ersoz S.; Turhan A.; Konca C.; Tezcaner T.; Erkent M.; Aydin O.; Avci T.; Altiner S.; Osmanov I.; Emral A.C.; Cetinkaya G.; Lapsekili E.; Sakca M.; Cimen S.; Ozen D.; Kozan E.B.; Dogan L.; Haberal E.; Kayhan O.; Aksel B.; Karabacak H.; Azili C.; Yazici F.; Apaydin M.; Kaya I.O.; Cetinkaya E.; Akin T.; Gunes G.; Turap H.; Aslan D.; Demirbag A.E.; Bolukbasi B.; Karaca B.E.; Ozturk E.; Ozeller E.; Kayacan G.S.; Borcek A.O.; Ece I.; Yormaz S.; Colak B.; Calisir A.; Sahin M.; Arslan K.; Hasirci I.; Ulutas M.E.; Metin S.H.; Gultekin F.A.; Ozkan Z.; Ilhan O.; Gundogdu T.; Liman R.K.; Kanat B.H.; Aydin A.; Sungurtekin U.; Ozgen U.; Aykota M.R.; Altintoprak F.; Gonullu E.; Cakmak G.; Dulger U.C.; Mantoglu B.; Demir H.; Akin E.; Eroz E.; Nazli O.; Dere O.; Dadasoglu M.A.; Kara E.; Tutcu S.; Solak I.; Gencer I.; Dalkiran A.; Sevinc B.; Karahan O.; Damburaci N.; Sari E.; Akay T.; Calta A.F.; Ozdemir A.; Ohri N.; Ermis I.; Bozbiyik O.; Ozdemir M.; Goktepe B.; Demir B.; Kilincarslan O.; Gunduz U.R.; Olcum M.; Dincer O.I.; Cakir R.C.; Dinc B.; Sahin E.; Uludag E.; Arslan Y.; Posteki G.; Oktay A.; Tatar O.C.; Guler S.A.; Utkan N.Z.; Tayar S.; Copelci Y.; Kartal M.; Kalayci T.; Yeni M.; Buyukkasap A.C.; Vural S.; Kesicioglu T.; Aydin I.; Gulmez M.; Saracoglu C.; Topcu O.; Kurt A.; Soylu S.; Kurt B.; Serin M.; Basceken S.I.; Gundes E.; Savda M.; Balkan A.Z.A.; Yildiz M.N.; Uzunkoy A.; Karaca E.; Berkan A.; Isik A.; Yildiz Y.A.; Ergul Z.; Yasar N.F.; Badak B.; Ozen A.; Velipasaoglu M.; Ure I.Background: Appendicitis is the most prevalent surgical emergency. The negative appendicectomy rate and diagnostic uncertainty are important concerns. This study aimed to assess the effectiveness of current appendicitis risk prediction models in patients with acute right iliac fossa pain. Methods: A nationwide prospective observational study was conducted, including all consecutive adult patients who presented with right iliac fossa pain. Diagnostic, clinical and negative appendicectomy rate data were recorded. The Alvarado score, Appendicitis Inflammatory Response (AIR), Raja Isteri Pengiran Anak Saleha Appendicitis (RIPASA) and Adult Appendicitis Score systems were calculated with collected data to classify patients into risk categories. Diagnostic value and categorization performance were evaluated, with use of risk category-based metrics including 'true positive rate' (percentage of appendicitis patients in the highest risk category), 'failure rate' (percentage of patients with appendicitis in the lowest risk category) and 'categorization resolution' (true positive rate/failure rate). Results: A total of 3358 patients from 84 centres were included. Female patients were less likely to undergo surgery than men (71.5% versus 82.5% respectively; relative risk 0.866, 95% c.i. 0.834 to 0.901, P < 0.001); with a three-fold higher negative appendicectomy rate (11.3% versus 4.1% respectively; relative risk 2.744, 95% c.i. 2.047 to 3.677, P < 0.001). Ultrasonography was utilized in 56.8% and computed tomography in 75.2% of all patients. The Adult Appendicitis Score had the best diagnostic performance for the whole population; however, only RIPASA was significant in men. All scoring systems were successful in females patients, but Adult Appendicitis Score had the highest area under the receiver operating characteristic curve value. The RIPASA and the Adult Appendicitis Score had the best categorization resolution values, complemented by their exceedingly low failure rates in both male and female patients. Alvarado and AIR had extremely high failure rates in men. Conclusion: The negative appendicectomy rate was low overall, but women had an almost three-fold higher negative appendicectomy rate despite lower likelihood to undergo surgery. The overuse of imaging tests, best exemplified by the 75.2% frequency of patients undergoing computed tomography, may lead to increased costs. Risk-scoring systems such as RIPASA and Adult Appendicitis Score appear to be superior to Alvarado and AIR. © 2024 The Author(s). Published by Oxford University Press on behalf of BJS Foundation Ltd.Item Electrophysiological detection of exam stress in health schools' students(Akademiai Kiado ZRt., 2024) Kazdagli H.; Ozel H.F.; Ozturk S.; Ceylan D.; Erdeniz B.; Ozbek M.; Semin M.I.Anxiety is a common issue among university students, many of them experience anxiety, depression, and stress during their school life. This study aimed to compare the acute physiological stress responses of students divided into two groups according to their perceived anxiety levels (positive test anxiety, PTA+, and negative test anxiety, PTA-). Heart rate variability (HRV) and electrodermal activity (EDA) were used to assess stress. Thirty-one healthy volunteers participated in the study. Participants completed anxiety assessments, including the Westside Test Anxiety Scale (WTAS), the State-Trait Anxiety Inventory (STAI), and the Test State Anxiety Inventory (TSAI). Based on their scores, participants were categorized into PTA+ and PTA-groups. All participants underwent 24-h continuous recordings of pulse and electrodermal activity (EDA) on two separate occasions: one day prior to a written exam and during a designated exam-free day serving as a baseline control. We compared the HRV and EDA data obtained on a regular day and on an exam day between the two groups. Results showed that the PTA+ group had significantly higher heart rate, stress index, low frequency, and short-Term detrended fluctuation analysis (DFAα1) on the exam day. The tonic EDA component was also higher in the PTA+ group. Stress-related HRV and EDA parameters were negatively correlated with exam scores. In conclusion, the study found that physiological stress indicators obtained from HRV and EDA are associated with perceived exam anxiety in students. © 2024 Akadémiai Kiadó, Budapest.Item Therapeutic implications of etiology-specific diagnosis of early-onset developmental and epileptic encephalopathies (EO-DEEs): A nationwide Turkish cohort study(W.B. Saunders Ltd, 2024) Kanmaz S.; Tekgul H.; Kayilioglu H.; Atas Y.; Kart P.O.; Yildiz N.; Gumus H.; Aydin K.; Olculu C.B.; Dogan D.E.T.; Per H.; Canpolat M.; Gulec A.; Yildirim N.; Turk E.; Celik N.; Ozturk S.; Kumandas S.; Kilic B.; Topcu Y.; Ozpinar E.; Coskun A.; Arslan M.; Akkoyunlu D.S.; Cine N.; Uzan G.S.; Gunay C.; Akyol D.; Ersoy O.; Direk M.C.; Komur M.; Kirkgoz H.; Karaoğlu P.; Ibis I.B.P.; Cerci C.; Orak A.; Oktay S.; Ayanoglu M.; Yildirim M.; Bektas O.; Serdaroglu E.; Yilmaz S.B.; Cankurt I.; Hirfanoglu T.; Arhan E.; Gencpinar P.; Dundar N.O.; Teber S.; Serin H.M.; Yilmaz S.; Tosun A.; Polat M.; Yilmaz U.; Unalp A.; Kara B.; Okuyaz C.; Yis U.; Hiz S.; Aktan G.; Gokben S.; Unay B.; Serdaroglu A.; Cansu A.Objective: To evaluate the etiology-specific diagnosis of early-onset developmental epileptic encephalopathies (EO-DEEs) in a nationwide Turkish cohort to determine the implications for therapeutic management. Methods: The cohort comprised 1450 patients who underwent EO-DEE. The utility of genetic testing was assessed with respect to the initial phases of next generation sequencing (NGS) (2005–2013) and the current NGS era (2014–2022). A predefined four-stepwise diagnostic model was evaluated using cost-effectiveness analysis. The diagnostic and potential therapeutic yields of the genetic tests were subsequently determined. Results: Gene-related EO-DEEs were identified in 48.3 % (n = 701) of the cohort: non-structural genetic (62.6 %), metabolic genetic (15.1 %), and structural genetic (14.1 %). The most common nonstructural genetic variants were SCN1A (n = 132, 18.8 %), CDKL5 (n = 30, 4.2 %), STXBP1 (n = 21, 2.9 %), KCNQ2 (n = 21, 2.9 %), and PCDH19 (n = 17, 2.4 %). The rate of ultra-rare variants (< 0.5 %) was higher in the NGS era (52 %) than that in the initial phase (36 %). The potential therapeutic yields with precision therapy and antiseizure drug modification were defined in 34.5 % and 56.2 % in genetic-EO-DEEs, respectively. The diagnostic model provided an etiology-specific diagnosis at a rate of 78.7 %: structural (nongenetic) (31.4 %), genetic (38.5 %), metabolic (6.1 %), and immune-infectious (2.8 %). Based on a cost-effectiveness analysis, the presented diagnostic model indicated the early implementation of whole-exome sequencing for EO-DEEs. Significance: In the present cohort, the higher rate (48.3 %) of gene-related EO-DEE diagnoses in the NGS era provides a potential therapeutic management plan for more patients. © 2024 British Epilepsy Association