Browsing by Author "Parlak Y."
Now showing 1 - 20 of 32
Results Per Page
Sort Options
Item Assessment of dose rates around Manisa (Turkey)(2006) Ereeş F.S.; Aközcan S.; Parlak Y.; Çam S.Natural radionuclide activity concentration of surface soils at 64 locations in central Manisa were measured using gamma spectrometry (ORTEC). The natural gamma radioactivity of the terrestrial radionuclides in soil samples and the gamma-absorbed dose rates of these radionuclides in air were calculated. In this study, track etch film (CR-39) was used to determine the distribution of radon levels in dwellings. The average annual effective dose equivalents from the calculated outdoor terrestrial gamma radiation for a person in Manisa is 66 μ Sv, whilst the annual effective dose equivalent from 222Rn is calculated to be 4.83 mSv/yr. © 2006 Elsevier Ltd. All rights reserved.Item Effects of 93m93m and 33m33mTc-MDP administration on dual-energy X-ray absorptiometry bone mineral density measurements(2009) Gumuser G.; Parlak Y.; Topal G.; Aras F.; Ruksen E.; Sayit E.Objective Nuclear medicine procedures are often performed in close-time proximity to bone densitometry studies. The purpose of this study was to determine the effects of 99m99mTc-hexakis-2-methoxyisobutyl isonitrile (MIBI) and 99m99mTc-methylene diphosphonate (MDP) on the accuracy of bone mineral density (BMD) measurements performed using dual-energy X-ray density. Methods The effect of a diagnostic dose of 99m 99mTc-MIBI on BMD estimations in the lumbar spine and the left total hip was assessed in 30 patients (19 female, 11 male; mean age: 55.5 ±±10.5 years) by using a Lunar DPX-NT scanner. Thirty patients, admitted to the nuclear medicine department for bone scintigraphy (15 female, 15 male; mean age: 56 ± 15.92 years), were included into the study. Each patient underwent dual-energy X-ray density assessment for which a Lunar DPX-NT scanner was used before and 2 h after intravenous injection of 99m 99mTc-MDP (925MBq) and 99m99mTc-MIBI (1110MBq). BMD measurements were calculated from lumbar spine (including L2-4) and left hip (including femoral neck, trochanter, and total hip). For statistical analysis, the Wilcoxon test was used and a P value of less than 0.05 was accepted as statistically significant. Results According to Wilcoxon's statistical test, we found extremely significant changes on the measured BMD, T-score, before and 2h after the injection of 99m99mTc-MIBI for lumbar spine and left hip in 30 patients. We found statistically significant decrement on measured BMD from lumbar spine and trochanter before and 2 h after the injection of 99m 99mTc-MDP. Although MDP BMD values in femoral neck and total hip were decreased after the injection of Tc-99m, they did not reach a statistically significant value. The comparison of pre-T-score and post-T-score values showed a statistically significant decrease after the injection for only L2-4 lumbar spine (P= 0.002), but left hip of pre-T-score and post-T-score values did not reach a statistically significant value. © 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins.Item Synthesis, radiolabeling and in vivo biodistribution of diethylstilbestrol phosphate derivative (DES-P)(2012) Ünak P.; Biber Müftüler F.Z.; Içhedef Ç.; Medine E.I.; Özmen K.; Ünak T.; Kilçar A.Y.; Gümüşer F.G.; Parlak Y.; Bilgin E.S.Diethylstilbestrol (DES) is a well known, nonsteroidal estrogen with high affinity for the estrogen receptor (ER). Today DES is used to treat breast and prostate cancers. A phosphate derivative of DES [Diethylstilbestrol diphosphate (DES-P)] which is specific to tumor cells consisting alkaline phosphatase enzyme was synthesized and labeled with 99mTc using tin chloride as reducing agent. In vivo biological activity of 99mTc labeled diethylstilbestrol phosphate compound ( 99mTc-DES-P) was examined by biodistribution studies on Wistar Albino rats. Statistical evaluation was performed using SPSS 13 program. The percentage (%) radiolabeling yield of 99mTc-DES-P and quality control studies were done by Thin Layer Radio Chromatography (TLRC). Results showed that, 99mTc-DES-P may be proposed as an imaging agent for ER enriched tumors such as uterus and prostate and their metastases in bones. © 2012 Akadémiai Kiadó.Item Assessment of lung toxicity caused by bleomycin and amiodarone by Tc-99m HMPAO lung scintigraphy in rats(2013) Gumuser G.; Vural K.; Varol T.; Parlak Y.; Tuglu I.; Topal G.; Sayit E.Aim: The purpose of the study was to determine the lung toxicity caused by amiodarone (AD) and bleomycin (BLM) in rats, by means of Tc-99m HMPAO lung scintigraphy. Methods: Thirty albino rats were randomly divided into five groups. After AD or BLM was dissolved with isotonic saline (SF), a 0.5 ml solution was applied to the right bronchus via a catheter. Group 1 (n = 5 rats) received a single dose of AD, group 2 (n = 5) received two doses of AD, group 3 (n = 9) received BLM, group 4 (n = 3) received hydrochloric acid (HCl), and group 5 (n = 8) received SF. Rats in groups 1, 2, 3 and 5 were given 37 MBq Tc-99m HMPAO from the tail vein on days 7, 14, 21 and 28, and at 4 and 24 h in group 4. Static images of 10 min duration were obtained at 30 and 60 min by a double-headed gamma camera (Infinia, GE, Tirat Hacermel, Israel) on 256 × 256 matrix. Regular regions of interests were drawn over the right lung (RL), left lung (LL) and the liver (Li), and lung/liver (L/Li) ratios were calculated. After the scintigraphic imaging procedures were completed, rats were killed. Lung tissues were evaluated on a scale of (+) to (+++++) for edema, alveolar structural integrity and infiltration by inflammatory cells. Results: Groups 2 and 3 showed statistically significant differences in RL/Li and LL/Li ratios, whereby RL/Li was higher than LL/Li (p < 0.05). There were no significant differences in RL/Li and LL/Li ratios in group 5 (p > 0.05). In histopathological examination, minimal damage or artifacts were observed in group 5. In group 4, almost all pathological findings were present in the right lung. Statistically significant (p < 0.01) histological differences were found when groups 1 and 5 were compared. More significant (p < 0.001) pathological effects were noted when groups 2 and 3 were compared to both groups 5 and 1. Injury was more prominent in the lung tissues of the control rats that were given HCl. Increased RL/Li ratios and histopathological findings were consistent. Conclusion: Tc-99m HMPAO lung scan are found to be useful in the identification of patients with lung toxicity. The simplicity of the procedure and lower radiation exposure are the advantages of Tc-99m HMPAO lung scan. © 2013 The Author(s).Item The evaluation of urine activity and external dose rate from patients receiving radioiodine therapy for thyroid cancer(2013) Demir M.; Parlak Y.; Çavdar I.; Yeyin N.; Tanyildizi H.; Gümüşer G.; Sayit E.; Erees S.; Sayman H.The aim of this study was to determine the external dose rate of iodine retention as a function of time in the bodies of thyroid cancer patients during their isolation period in the hospital. Urine samples were collected at 6th, 12th, 18th, 24th h and 2nd, 3rd, 4th, 5th d from 83 patients after oral administration of 131I and counted. The external dose rates were also simultaneously determined at the same time points. Then, it was expressed as retained radioiodine body activity versus dose rate. Effective half life calculated from urine sample measurements was found as 18.4±1.8 h within the first 24 h and 64±2.7 h between 48 and 120 h. According to this results, the external dose rate (<20 μSv h-1), which patients could be discharged, was achieved after 48 h for 3700 and 5550 MBq, and after 72 h for 7400 MBq of131I treatments. © The Author 2013. Published by Oxford University Press. All rights reserved.Item Samarium-153 therapy for prostate cancer: The evaluation of urine activity, staff exposure and dose rate from patients(Oxford University Press, 2015) Parlak Y.; Gumuser G.; Sayit E.The aim of this study was to determine the excretion of Samarium-153-ethylenediaminetetramethylphosphonic acid (153Sm- EDTMP) in urine and to calculate the dose rate of its retention in the body as a function of time and the dose received by the skin of laboratory staff 's finger. Urine samples were collected from 11 patients after intravenous injection of 153Sm-EDTMP. The measurements of dose rate were performed. Thermoluminescent dosemeters were used for absorbed dose measurements. Effective half-lives that were calculated from urine sample measurements were found as 7.1±3 h within the first 24 h. Whole body dose rates before collecting urine of patients were 60.0 ± 15.7 μSv h21 for within 1 h following 153Sm-EDTMP administration. The highest finger radiation dose is to the right-hand thumb (3.8 ± 2 mGy). The results of the study imply that patients who recieved 153Sm-EDTMP therapy should be kept a minumum of 8 h in an isolated room at hospital and that one staff should give therapy at most two patients per week. © The Author 2014.Item F-18 FDG PET/CT imaging artifacts; [F-18 FDG PET/BT görüntüleme artefaktları](Istanbul Tip Fakultesi, 2015) Parlak Y.; Gümüşer G.; Sayit Bilgin B.E.F-18 FDG PET/CT are being used in increasing numbers in the diagnosis of the primary lesion, disease staging and evaluation of response to therapy of various cancers due to its high accuracy. However, PET/CT have various artifacts which affected image quality including PET and CT AC (attenuation correction) artifacts. For accurate identification of malignant lesions, to characterize these artifacts is clinically important. Our aim is to identify, to characterize and to investigate the effect of PET/CT image quality of these artifacts. © 2015 Onkoloji Derneği.Item Cytogenetic effects of 99technetium on meristematic cells of root tips of Vicia faba L. andstatistical comparison(Taylor and Francis Ltd., 2015) Özdemir A.; Bozdağ B.; Sepet H.; Parlak Y.; Kocabaş O.; Ereeş F.S.; Özdemir C.In this study, cytogenetic effects of 99technetium (99Tc) on meristematic cells of root tips belonging to Vicia faba L. have been investigated. Seeds of the plant were prepared and kept in 99Tc standard for different time periods: 1/12, 1/4, 1/2, 1, 2, 3, 4, 5, 6 and 12 h. Seeds treated with 99Tc were sprouted and the root tips obtained were prepared for microscopic examination. Some abnormalities e.g. chromosome breaking, chromosome dispersion, bridge chromosomes, chromosome adherence and ring chromosomes were observed. Abnormalities seen for each treatment depended on the time period. The variety and number of abnormalities usually increased with increased treatment time. The results obtained were evaluated statistically. © 2015 Dipartimento di Biologia Evoluzionistica, Università di Firenze.Item Bone marrow radiation dosimetry of high dose 131I treatment in differentiated thyroid carcinoma patients(Novin Medical Radiation Institute, 2016) Parlak Y.; Demir M.; Cavdar I.; Ereees S.; Gumuser G.; Uysal B.; Kaya G.C.; Koç M.; Sayit E.Background: Radiation absorbed dose to the red bone marrow, a critical organ in the therapy of thyroid carcinoma, is generally kept below 2 Gy for non-myeloablative therapies. The aim of this study was to calculate bone marrow radiation dose by using MIRDOSE3 package program and to optimize the safe limit of acivity to be administered to the thyroid cancer paients. Materials and Methods: In this study, 83 thyroid cancer patients were divided into 3 groups based on the amount of acivity administered into the body. In the groups, 3700 MBq, 5550 MBq and 7400 MBq activities were used respecively. The curves of ime-acivity were drawn from blood samples counts and effective half-life and residence time were calculated. Correlations of bone marrow radiation dose and radioiodine effective half-life were determined as a function of administered activity via ANOVA test. Tg levels and tumour diameters were compared using Spearman's correlation. Results: The effective half-lives of 131I for three groups of whole-body, receiving 3700 MBq, 5550 MBq and 7400 MBq were calculated as 20.57±5.4, 17.8±5.8 and 18.7±3.9 hours, respectively. The average bone marrow doses for 3 groups of patients were 0.32±0.08 Gy, 0.42±0.14 Gy and 0.60±0.24 Gy, respectively. Conclusion: It was concluded that, the bone marrow dose to the patients still remains within the recommended level even after administering an activity of 7400 MBq of 131I to the patients.Item Synthesis, characterization and radiolabeling of folic acid modified nanostructured lipid carriers as a contrast agent and drug delivery system(Elsevier Ltd, 2017) Ucar E.; Teksoz S.; Ichedef C.; Kilcar A.Y.; Medine E.I.; Ari K.; Parlak Y.; Sayit Bilgin B.E.; Unak P.Nanostructured lipid carriers (NLCs) are the new generation of solid lipid drug delivery systems. Their suitability as contrast agents for gamma scintigraphy is an attracting major attention. The aim of current study was to prepare surface modified nanostructured lipid carrier system for paclitaxel (PTX) with active targeting and imaging functions. In accordance with the purpose of study, PTX loaded nanostructured lipid carriers (NLCs) prepared, modified with a folate derivative and radiolabeled with technetium-99 m tricarbonyl complex (99 mTc(CO)3 +). Cellular incorporation ratios of radiolabeled nanoparticles (99 mTc(CO)3-PTX-NLC) were investigated in vitro on three cancer cell lines. Additionally in vivo animal studies conducted to evaluate biological behavior of 99 mTc(CO)3-PTX-NLC on female Wistar Albino rats. Biodistribution results showed that the folate derivative modified 99 mTc(CO)3-PTX-NLC had considerably higher uptake in folate receptor positive organs. The data obtained from present study could be useful in the design of biodegradable drug carriers of PTX and folate receptor based tumor imaging agents. © 2016 Elsevier LtdItem Radiosynthesis and biodistribution of 99mTc-Sulfamethoxazole: a novel molecule for in-vivo infection imaging(Birkhauser Boston, 2017) Uyaroğlu Ö.; Demiroğlu H.; Topal G.; Parlak Y.; Gül Gümüşer F.; Uluer Türköz E.; Demir V.; Ateş B.; Ünak P.; Avcıbaşı U.The aim of this study was to prepare 99mTc-Sulfamethoxazole complex and evaluate its efficiency as an infection imaging agent. The Sulfamethoxazole was labeled with 99mTc and its biological efficacy as a potential radio tracer for Staphylococcus aureus infection was investigated in bacterially infected Albino Wistar rats. The radiolabeling yield was found to be 95 ± 3.07% and remained constant at more than 93 ± 0.1% even in serum for 240 min after radiolabeling. 99mTc-Sulfamethoxazole prepared with high yield localized well in the bacterially infected muscle of the rats. 99mTc-Sulfamethoxazole may be developed as a radiopharmaceutical agent to distinguish infection from inflammation by nuclear imaging. © 2017, Springer Science+Business Media, LLC.Item Radiosynthesis and biodistribution of 99mTc-trimethoprim: A novel radiolabeled antibiotic for bacterial infection imaging using experimental animals; [ 99mTc-Trimethoprim’in radyosentezi ve biyodağılımı: Deney hayvanları kullanılarak bakteriyel enfeksiyon görüntüleme için yeni bir radyoişaretli antibiyotik](Veteriner Fakultesi Dergisi, 2018) Demiroğlu H.; Topal G.; Parlak Y.; Gümüşer F.G.; Türköz E.U.; Tekin V.; Ateş B.; Ünak P.; Avcibaşi U.In the present article, we focused on the radiolabeling and evaluation of 99mTc–TMH complex as a potential candidate for infection imaging in vivo. For this; Trimethoprim (TMH) used to treat bacterial infections was investigated to label with99mTc. Labeling was performed using thin (II) chloride as a reducing agent at room temperature for 1 h and radiochemical analysis involved thin layer radiochromatography (TLRC) and high pressure liquid radiochromatograpy (HPLRC) methods. The stability of labeled antibiotic was checked in the presence of rat blood serum at 37.C up to 180 min. The maximum radiolabeling yield was found to be 96±2% and remained constant at more than 85±1% even in rat serum for 180 min after radiolabeling. Static image of 99mTc-TMH in male rats demonstrated that important radiation signals are present in the infected site at first glance in 30 min. After 30 min the uptake of the 99mTc-TMH as ID/g% in the infected muscle (INM) and normal muscle (NM) of the rats were 7.5±1.5% and 5.00±1.2%, respectively. In the INM/NM ratio a desirable behavior was observed as the values for the INM/ NM increased up to 10.6. 99mTc-TMH prepared with high yield is able to localize well in the bacterially infected muscle of the rats. As a result,99mTc-TMH may be developed as a radiopharmaceutical agent to distinguish infection from inflammation by nuclear imaging. © 2018, Veteriner Fakultesi Dergisi. All rights reserved.Item One-step conjugation of glycylglycine with [18F]FDG and a pilot PET imaging study(Springer Netherlands, 2018) Şenk A.M.; İçhedef Ç.; Kılçar A.Y.; Uçar E.; Arı K.; Göksoy D.; Parlak Y.; Sayıt Bilgin B.E.; Teksöz S.This study describes a single step conjugation of Glycylglycine (GlyGly) which is a small peptide, with [18F]FDG via oxime formation. Amiooxy-functionalization of GlyGly (AO-GlyGly) was accomplished through the reaction of Boc-aminooxy succinimide ester. Conjugation reaction was performed at 100 °C for 30 min in a vial containing AO-GlyGly and [18F]FDG solution. The radiolabeled product ([18F]FDG-GlyGly) was obtained with 98.65 ± 0.35% yield without any purification step which makes this method more attractive for 18F radiolabeling. The present study is concluded with an in vivo pilot animal PET study to assess biodistribution and kinetics of chemoselectively [18F]FDG tagged GlyGly in vivo. © 2018, Akadémiai Kiadó, Budapest, Hungary.Item Evaluation of new 99mTc(CO)3 + radiolabeled glycylglycine In Vivo(Bentham Science Publishers, 2019) Şenışık A.M.; İçhedef Ç.; Kılçar A.Y.; Uçar E.; Arı K.; Parlak Y.; Bilgin E.S.; Teksöz S.Background: Peptide-based agents are used in molecular imaging due to their unique properties, such as rapid clearance from the circulation, high affinity and target selectivity. Many of the radiolabeled peptides have been clinically experienced with diagnostic accuracy. The aim of this study was to investigate in vivo biological behavior of [99mTc(CO)3(H2O)3]+ radiolabeled glycylglycine (GlyGly). Methods: Glycylglycine was radiolabeled with a high radiolabeling yield of 94.69±2%, and quality control of the radiolabeling process was performed by thin layer radiochromatography (TLRC) and High-Performance Liquid Radiochromatography (HPLRC). Lipophilicity study for radiolabeled complex (99mTc(CO)3-Gly-Gly) was carried out using solvent extraction. The in vivo evaluation was performed by both biodistribution and SPECT imaging. Results: The high radiolabelling yield of 99mTc(CO)3-GlyGly was obtained and verified by TLRC and HPLRC as well. According to the in vivo results, SPECT images and biodistribution data are in good accordance. The excretion route from the body was both hepatobiliary and renal. Conclusion: This study shows that 99mTc(CO)3-GlyGly has the potential to be used as a peptide-based imaging agent. Further studies, 99mTc(CO)3-GlyGly can be performed on tumor-bearing animals. © 2019 Bentham Science Publishers.Item 99mTc(I) carbonyl-radiolabeled lipid based drug carriers for temozolomide delivery and bioevaluation by in vitro and in vivo(Walter de Gruyter GmbH, 2019) Arı K.; Uçar E.; İçhedef Ç.; Kılçar A.Y.; Medine E.İ.; Parlak Y.; Bilgin B.E.S.; Aydın B.; Gümüşer F.G.; Teksöz S.In preclinical research radiolabeled nanoparticles have been attracting interest as a new class of imaging probes. Assuming good stability of solid lipid nanoparticles (SLNs) under physiological conditions, radiolabeled SLNs can be used for imaging and measuring uptake in target tissue. Present study was performed to evaluate biological behavior of temozolomide (TMZ) loaded solid lipid nanoparticles (SLN-TMZ) in vivo and in vitro. Lipid nanoparticles were prepared by emulsification and low-temperature solidification method. ζ potential, morphology and particle size of nanoparticles were determined. Biological behavior of 99mTc(CO)3+ radiolabeled SLN-TMZ were investigated in vitro on U87/Daoy cell lines and in vivo on female Wistar Albino rats. Obtained results of in vitro incorporation, in vivo biodistribution and gamma imaging studies on radiolabeled SLN-TMZ show that the radiolabeled solid lipid nanoparticles could have potential as a drug delivery system for TMZ. © 2019 Walter de Gruyter GmbH. All rights reserved.Item Acceptance Tests and Quality Control of the Positron Emission Tomography (PET) Systems; [Pozitron Emisyon Tomografi (PET) sistemlerinin kalite kontrolü ve kabul Testleri](Galenos Publishing House, 2020) Hacıosmanoğlu T.; Demir M.; Toklu T.; Suna Kıraç F.; İnce M.; Parlak Y.; Kovan B.; Dönmez S.; Ayan A.; Özaslan İ.A.; Hiçürkmez M.; Yeyin N.; Poyraz L.Quality control (QC) applications are important for the reliability and accuracy of the results obtained from positron emission tomography (PET) imaging systems in clinical trials. With these applications, exposure of patients and employees to more radiation will be prevented, and it will be possible to detect the systems that lost their performance compared to their installation time. QC applications are grouped as acceptance and reference tests, routine periodic QC tests. PET performance tests standardized by National Electrical Manufacturers Association (NEMA) should be used as acceptance and reference tests. In addition, the performance of the device should be monitored by following these tests at six-month intervals. PET/computerized tomography (CT) manufacturers often recommend routine QC procedures for their equipment and require users to follow these recommendations to a minimum. If standards are not defined for routine QC procedures, it is recommended to comply with the general minimum standards for routine QC that all PET/CT centers must perform. Routine QC protocols, the image quality of the PET system; CT image quality and CT dose of the patient; the accuracy of CT-based attenuation corrections; the accuracy of the CT and PET co-registration should ensure that problems that can interact any of the performance characteristics are identified. Since performing PET/CT scans by equipment that does not comply with their specifications may compromise image quality, interpretation of images and reliability of results, daily QC parameters must be evaluated and necessary corrective procedures must be taken prior to scanning. The tests are carried out using the protocol provided by the manufacturer. © Telif Hakkı 2020 Türkiye Nükleer Tıp Derneği / Nükleer Tıp Seminerleri, Galenos Yayınevi tarafından yayınlanmıştır.Item Radiation Safety Guide: General Definitions and Radiation Protection Rules in Nuclear Medicine Applications(Galenos Publishing House, 2020) Parlak Y.; Uysal B.; Kıraç F.S.; Kovan B.; Demir M.; Ayan A.; Poyraz L.; Özaslan İ.A.; Köseoğlu K.; Yeyin N.; Dönmez S.Ionizing radiation which is widely used in the field of medicine and industry, and in scientific studies leaves some or all of its energy to the tissue as it passes through living tissue. It causes known possible harmful effects on living organisms, from low exposure doses to high doses. These are stochastic effects (cancer, mutations) and deterministic effects which are recently named as tissue reactions (such as dermatitis, cataracts). It is important to keep the radiation exposure of the radiation workers and the public as low as possible and to keep within the acceptable dose limits in order to avoid unknown harmful effects. Radiation risks to health are assessed by radiation absorbed dose, dose equivalent and effective doses. In routine daily practices, as low as reasonably achievable dose and as low as reasonably practible dose principles, and also, occupational and public permissible dose limits defined by international and national atomic energy agencies should be complied. Radiation protection rules should be followed to reduce an exposure dose as much as possible while working in the radiation areas. Radiation accidents, transportation of radioactive materials and radioactive waste disposal are important special issues for the protection of environmental health, and compliance with radiation protection rules are necessary. With the development of new targeted radiopharmaceuticals, radionuclide treatments are frequently applied in nuclear medicine clinics, especially in the treatment of malignant diseases. Therefore, radiation protection is an important issue especially for the hospital workers performing procedures to the patients who die shortly after administration of high-dose radionuclide therapy. In this guide, the terms related to radiation will be presented at first, and then, the national and international radiation protection rules in radiation works will be given. © Telif Hakkı 2020 Türkiye Nükleer Tıp Derneği / Nükleer Tıp Seminerleri, Galenos Yayınevi tarafından yayınlanmıştır.Item Acceptance and Quality Control Tests for the Single Photon Emission Computerized Tomography (SPECT) Gamma Cameras and SPECT/CT Systems; [Tek Foton Emisyon Kompüterize Tomografi (SPECT) Gama Kameralar ve SPECT/BT Sistemleri için Kabul ve Kalite Kontrol Testleri](Galenos Publishing House, 2020) Dönmez S.; Ayan A.; Parlak Y.; Kovan B.; Hiçürkmez M.; Kıraç F.S.; Demir M.; Toklu T.; Hacıosmanoğlu T.; Özaslan İ.A.; Poyraz L.; ve Kalite Kontrol Çalışma Grubu Üyeleri T.R.G.Scintigraphic images play an important role in the diagnosis of diseases by providing accurate clinical information independent of the operator. However, the good quality image may not be obtained due to changes in the performance of the imaging system and/or external factors. A poor quality image will make difficult to interpretate clinical studies. Quality Control (QC) tests provide to reveal the conditions that will adversely affect the image quality before starting of scintigraphic imaging, and to make the necessary arrangements. Although QC tests show relative changes depending on the brand and model of gama camera, there are tests that must be done routinely. For gamma cameras [planar and single photon emission computerized tomography (SPECT)] to be newly installed in the Nuclear Medicine Clinic, “Acceptance Tests” should be carried out after the installation. “Checks before clinical studies” before. © Telif Hakkı 2020 Türkiye Nükleer Tıp Derneği / Nükleer Tıp Seminerleri, Galenos Yayınevi tarafından yayınlanmıştır.Item Preparation of a 99mTc-labeled graft polymer and its in vitro and in vivo evaluation(Springer Science and Business Media B.V., 2021) Avcıbaşı U.; Türkyarar T.; Karadağ A.; Bakan B.; Yavaşoğlu N.Ü.K.; Kuşat K.; Akgöl S.; Gülcemal D.; Tekin V.; Müftüler F.Z.B.; Topal G.; Parlak Y.; Gümüşer F.G.The aim of this study is the synthesis of a novel 99mTc-labeld graft polymer and the biological evaluation of its in vitro and in vivo properties. To this end, a L-proline-graft-poly(HEMA) was prepared and labeled with 99mTc. The radiochemical yield of approximately the 99mTc-labeled compound amounted to 97 ± 2.3%. The cytotoxicity test revealed no cytotoxic effect after a 24- and 48-h incubation. The results of the hemolysis test showed that hemolysis was non-toxic with an effect level of less than 2%. Subsequently, the biodistribution in healthy rats was determined. High accumulation of the polymer was observed in the pancreas, thyroid and prostate. © 2021, Akadémiai Kiadó, Budapest, Hungary.Item Thymoquinone glucuronide conjugated magnetic nanoparticle for bimo-dal imaging and treatment of cancer as a novel theranostic platform(Bentham Science Publishers, 2021) İnce İ.; Müftüler Z.B.; Medine E.İ.; Güldü Ö.K.; Takan G.; Ergönül A.; Parlak Y.; Yıldırım Y.; Çakar B.; Bilgin E.S.; Aras Ö.; Göker E.; Ünak P.Background: Theranostic oncology combines therapy and diagnosis and is a new field of medicine that specifically targets the disease by using targeted molecules to destroy the cancer-ous cells without damaging the surrounding healthy tissues. Objective: We aimed to develop a tool that exploits enzymatic TQ release from glucuronide (G) for the imaging and treatment of lung cancer. We added magnetic nanoparticles (MNP) to enable magnetic hyperthermia and MRI, as well as 131I to enable SPECT imaging and radionuclide thera-py. Methods: A glucuronide derivative of thymoquinone (TQG) was enzymatically synthesized and conjugated with the synthesized MNP and then radioiodinated with 131I. New Zealand white rab-bits were used in SPECT and MRI studies, while tumor modeling studies were performed on 6–7-week-old nude mice utilized with bioluminescence imaging. Results: Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) spectra confirmed the expected structures of TQG. The dimensions of nanoparticles were below 10 nm and they had rather polyhedral shapes. Nanoparticles were radioiodinated with 131I with over 95% yield. In imaging studies, in xenograft models, tumor volume was significantly reduced in TQGMNP-treated mice but not in non-treated mice. Among mice treated intravenously with TQGMNP, xenograft tumor models disappeared after 10 and 15 days, respectively. Conclusion: Our findings suggest that TQGMNP in solid, semi-solid and liquid formulations can be developed using different radiolabeling nuclides for applications in multimodality imaging (SPECT and MRI). By altering the characteristics of radionuclides, TQGMNP may ultimately be used not only for diagnosis but also for the treatment of various cancers as an in vitro diagnostic kit for the diagnosis of beta glucuronidase-rich cancers. © 2021 Bentham Science Publishers.