Browsing by Author "Sönmez, PK"

Now showing 1 - 7 of 7
  • No Thumbnail Available
    Item
    The effect of Electro Magnetic Fields on Cell Behaviour
    Tuglu, MI; Gülbagça, F; Sal, DH; Saygili, S; Sönmez, PK; Aydemir, I; Özkut, M; Öztürk, S; Gümüsay, M; Kaya, A; Keskin, N
    The biologic effects of electomagnetic fields application on medical field has been increased. Their effect on medical treatment depends on cell behaviour which can be observed by in vivo and in vitro studies. Their effect on the stem cells and the cell lines and the organs of experimental animals with morphological alterations are helpful to understand cell behaviour. The behaviour of the cells can be analyse at ultrastructial level by scaning electron microscopy. Application of electomagnetic fields cause increase of proliferation, migrationand improvement of wound healing with beneficial effects while they cause oxidative stress and cell daeth including apoptosis for cancer cells in different types of cell line. The minimal side effect with maximum beneficial treatment of these products suggest that they could be very useful for clinical trials.
  • No Thumbnail Available
    Item
    Effects of Electromagnetic Fields Application on Experimental Varicocele Model in Rats
    Gülbagça, F; Sal, DH; Saygili, S; Sönmez, PK; Aydemir, I; Özkut, M; Öztürk, S; Gümüsay, M; Kaya, A; Tuglu, MI
    Varicocele is the most clinical condition which can be treated by surgery. The frequency in male is 20%. The half of infertile patient has this pathology which there is dilatation in plexus pampiniformis veins of testes. Varicocele causes increase of heat or increse of oxidative stres in seminifer tubules where they produce pathology for spermatogenesis. Low frequency magnetic fields are useful for the treatment of many area where they become important role. In this study the effect of low frequency magnetic fields on the experimental varicocele model of rat testes was investigated. The parameters for oxidative stres suggest eNOS and iNOS with apoptosis were evaluated by immunohistochemical staining. There was increaese in eNOS, iNOS and apoptosis by the varicocele experiment where there was testicular damage and low frequency magnetic fields caused decrease in oxidative stress and apoptosis. Application of magnetic fields can help the clinical varicocele pathologies either with treatment or supportive effect.
  • No Thumbnail Available
    Item
    Punicic Acid Inhibits Glioblastoma Migration and Proliferation via the PI3K/AKT1/mTOR Signaling Pathway
    Mete, M; Unsal, UU; Aydemir, I; Sönmez, PK; Tuglu, M
    Background: Punicic Acid (PA) is a polyunsaturated fatty acid that accounts for approximately 70%-80% of Pomegranate Seed Oil (PSO). PA possesses strong antioxidant, anti-inflammatory, anti-atherogenic effects, and anti-tumorigenic properties. Pomegranate extracts have been shown to have anticancer activity in many studies. However, there is no evidence for the effect of PSO on T98 glioblastoma cells. Therefore, the present study was the first to investigate the mechanisms induced by PA on T98 cells, which is one of the major compounds extracted from PSO. Methods: The effects of PA on cell viability; oxidative stress; and migration, proliferation, and apoptosis at the IC50 close were studied. Results: The proliferation and migration were inhibited in the treated group compared to the non-treated group by 9.85 mu l/ml PA. The difference was statistically significant (***p<0.001). Furthermore, PA-induced apoptosis in the T98 glioblastoma cells compared to non-treated group and the difference was statistically significant (***p<0.001). Apoptosis was determined via immunocytochemistry staining of caspase-3, caspase-9 and TUNEL methods. Apoptosis was checked by flow cytometry (using caspase 3 methods) and Scanning Electron Microscopy Analysis. We also investigated the potential signaling pathway underlying this apoptotic effect. The immunocytochemical stainings of PI3K/ Akt-1/ mTOR-1 demonstrated that Akt-1 staining was increased with PA treatment similar to mTOR-1 and PI3K staining (***p<0.001). These increases were statistically significant compared to the non-treated group. Conclusion: PA exhibited exceptional abilities as an anticancer agent against GBM cells. The use of punicic acid in combination with other drugs used in the treatment of glioblastoma may increase the efficacy of the treatment. This study provided a basis for future investigation of its use in preclinical and clinical studies.
  • No Thumbnail Available
    Item
    Antiapoptotic and proliferative effect of bone marrow-derived mesenchymal stem cells on experimental Asherman model
    Öztürk, S; Sönmez, PK; Özdemir, I; Topdagi, YE; Tuglu, MI
    Purpose: We investigated the effects of stem cell therapy as an alternative to surgical methods and medical treatments in endometrial injuries in Asherman syndrome (AS). Materials and Methods: In this study, AS model was created chemically in rats. The bone marrow-derived mesenchymal stem cells isolated from the tibia and femoral bone of male individuals of the same species (BMDSC) were given to female rats with asherman syndrome and the changes in the endometrium were evaluated by histopathological parameters. Asherman + medium, Asherman + niche, Asherman + BMDSCs, Asherman + BMDSCs + niche were formed in four groups. Results: It was observed that increased endometrial thickness, gland count and vascularization and decreased fibrous areas and apoptotic cell death with regeneration in epithelium and lamina propria in treatment groups. No histopathologic changes were observed in the right uterine horns, which were evaluated as control group.. Conclusion: BMDSCs and Niche applications can contribute to the clinic by reducing the formation of adhesion within the mechanisms causing infertility. These positive results are promising in terms of transporting Asherman studies to the clinic.It has been shown that BMDSCs and Niche may contribute to the clinic by treatment with adhesion molecules in mechanisms that cause infertility.
  • No Thumbnail Available
    Item
    Effects of verteporfin-mediated photodynamic therapy in breast cancer cells
    Sönmez, PK; Turhan, A; Öztatlici, M; Özbilgin, K
    Photodynamic therapy works with a photosensitizer that is stimulated when exposed to a light source of a specific wavelength and produces a form of oxygen that can be used in cancer treatments. In this study, we investigated the effect of laser on apoptosis on breast cancer cell lines (MDA-MB-231) treated with verteporfin in cell culture media. Verteporfin added MDA-MB-231 cells were incubated without light for 24 hours after applying laser light at a wavelength of 695 nm at an intensity of 50 J/cm2 at various times. Anti-proliferative effects were evaluated by immunoreactivity of anti-Bcl-2 and anti-Bax antibodies by immunocytochemical staining. When anti-Bax/Anti-Bcl-2 ratio are compared, the ratio of 1.5 in the control group cells decreases in short-term laser applications, while it approaches normal values in the 7th min after longterm laser application and reaches a very high value in the 9th min. Therefore, our results suggest that verteporfin-mediated PDT may be a potential combined therapy strategy against breast carcinoma by increasing apoptosis.
  • No Thumbnail Available
    Item
    Effects of stem cells applications on growth factors during implantation
    Aydemir, I; Sönmez, PK; Saygili, S; Sal, DH; Gülbagca, F; Özkut, M; Tuglu, MI
  • No Thumbnail Available
    Item
    The functional effect of stem cells on the reproductive organs
    Tuglu, I; Aydemir, I; Özkud, M; Firat, F; Öztürk, S; Sönmez, PK; Saygili, S; Sal, DH; Gülbagça, F