Browsing by Author "Salmanoglu, DS"

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    Melatonin and L-carnitin improves endothelial disfunction and oxidative stress in Type 2 diabetic rats
    Salmanoglu, DS; Gurpinar, T; Vural, K; Ekerbicer, N; Dariverenli, E; Var, A
    Vascular dysfunction is thought to play a major role in the development of diabetic cardiovascular disease. The roles of endothelial dysfunction, oxidative stress, and dyslipidemia will be considered. Melatonin as well as L-carnitine were shown to possess strong antioxidant properties. Diabetes induced with high fat diet (for 8 weeks) and multipl low doses intraperitoneal injection of STZ (twice, 30 mg/kg/d i.p). The diabetic animals were randomly assigned to one of the experimental groups as follows: Control group (C), high fat diet (HFD), STZ-induced diabetic group (HFD+STZ), HFD+STZ diabetic group received melatonin (10 mg/kg/d i.p), HFD+STZ diabetic group received L-carnitine (0.6 g/kg/d i.p), and HFD+STZ diabetic group received glibenclamide (5 mg/kg/d, oral). The serum fasting blood glucose, insulin, total cholesterol, HDL-cholesterol, LDL-cholesterol, triglyceride and malondialdehyde (MDA) levels were tested. Acetylcholine induced endothelium-dependent relaxation and sodium nitroprusside induced endothelium-independent relaxation were measured in aortas for estimating endothelial function. Also, glutathione peroxidase (GPx), superoxide dismutase (SOD) and nitric oxide (NO) levels activities were determined in rat liver. According to our results melatonin and L-carnitine treatment decreased fasting blood glucose, total cholesterol, and LDL levels. MDA levels significantly decreased with the melatonin treatment whereas SOD levels were not significantly changed between the groups. The results suggest that especially melatonin restores the vascular responses and endothelial dysfunction in diabetes. (C) 2015 The Authors. Published by Elsevier B.V.
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    Melatonin and L-carnitine ameliorates endothelial dysfunction in type 2 diabetic rats
    Salmanoglu, DS; Gurpinar, T; Vural, K; Ekerbicer, N; Dariverenli, E; Var, A
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    Doxorubicin functionalized metallic nanoparticles for anticancer and antibacterial applications
    Yesil, I; Atmaca, H; Degirmenci, S; Çamli-Pulat, C; Ari, M; Tepe, M; Küçük, A; Salmanoglu, DS; Sancak, Z; Kilinç, C; Ilhan, S; Yazgan, I
    In this study, we used Doxorubicin, an FDA approved drug possessing anticancer and antibacterial activity, as a model drug to functionalize sugar ligand synthesized silver/gold bimetallic nanoparticles (Ag/AuNPs) to test simultaneous antibacterial and anticancer activities under in vitro conditions. DU-145 prostate cancer cells were used as the main target while T98G glioblastoma and MDA-MB-231 breast cancer cell lines were used for selectivity test, and HEK-293 epithelial cell line was used as non-cancerous cells to test toxicity of these formulations. Three Ag/AuNP and corresponding Doxorubicin (D) functionalized ones and free Doxorubicin were tested for these four cell lines. Plain Ag/AuNP showed the highest toxicities on HEK-293 cell lines while DU-145 cell line showed the greatest vulnerability for Ag/AuNP-D formulations. However, DU-145 cells showed the lowest susceptibility for free Doxorubicin at all the tested concentrations. The antiproliferative activity was not dose dependent while an inverse relationship was obtained for a certain concentration range. Two Doxorubicin functionalized gold nanoparticles (AuNP-D) were then synthesized and applied on DU-145 cells. Interestingly, a better anticancer activity was obtained even at the minimum applied concentration (1.8 x 10-5 mu g/mL D). Antibacterial activities of these formulations were also tested for multidrug-resistant gram (-) and gram (+) bacterial species. Depending on the sugar ligand chemistry, antibacterial activity of Doxorubicin functionalized Ag/AuNPs showed a better performance in comparison to the plain Ag/AuNPs and vice-versa. Based on the results, it can be claimed that selective or semi-selective formulations targeting cancer cells and bacterial species as anti-neutropenia formulations can be developed using carbohydrate derivatives synthesized metallic nanoparticles as drug delivery agents.