Browsing by Author "Saygili S."

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    Compression of the effect of pulsed electromagnetic field and pulsed radio frequency energy on wound healing in rats; [Darbeli elektromanyetik alan ve darbeli radyofrekans uygulamalarinin siçanlarda yara iyileşme etkilerinin karşilaştirmasi]
    (Institute of Electrical and Electronics Engineers Inc., 2017) Gumusay M.; Gulbagca F.; Saygili S.; Aydemir I.; Kaya A.; Tuglu M.I.
    Delayed or not sufficiently good wound healing is a significant problem in medical treatment is needed to support in order to improve healing. Low frequency electromagnetic field (EMF) applications are seems to give this support. Disturbed vascularization, connective tissue formation, matrix exchange, and inflammation process in the wound environment are modified after EMF applications compared with control groups. Deteriorations in these factors which can be returned with the electrical impulses, DNA synthesis, transcription and transforms of protein synthesis, increasing of fibroblasts and endothelial cell proliferation indicate the presence of EMF mechanisms. Since the application and ambient conditions are kept constant but the type of the application has been changed in our study, it was provided that pulsed radio frequency energy (PRFE) is more effective and has more long-term improvement compared to pulsed electromagnetic fields (PEMF). The effects of these applications on cells and understanding of the mechanisms of the behavior will be much more significant and meaningful contribute to the treatment. © 2016 IEEE.
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    Development of electromagnetic field system for providing wound healing on created wound model rat skin and sensor application; [Sicąn Derisinde Olusturulan Yara Modeli Üzerinde Iyilesme Saglanmasi için Elektromanyetik Alan Sistemi Gelistirilmesi ve Sensör Uygulamasi]
    (Institute of Electrical and Electronics Engineers Inc., 2017) Gumusay M.; Gulbagca F.; Aydemir I.; Saygili S.; Kaya A.; Tuglu M.I.
    Electromagnetic field (EMF) radiation are formed by the acceleration of electric charge. Electric charges produce electric and magnetic energy in space when they move. These waves have different frequencies and wavelengths and when they interact with an object they can be refracted, reflected or absorbed. When a suitable frequency and waveform EM signal is applied to the target tissue with the help of a coil or applicator physiological effects will occur at the cellular size. Today studies revealed significant molecular mechanisms occurring during wound healing. Non-ionizing part of the EM spectrum is low-frequency electromagnetic fields are used as adjunct therapy in soft tissue injuries. In this study, the main goal is development of systems which will give better results compared with conventional treatment methods taking into account the effect on rat skin. To achieve this, some improvements in output power, increased gain, versatility, easy adjustments are provided for the spiral and planar antenna-applicator design. © 2016 The Chamber of Turkish Electrical Engineers.
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    Design of coils driven by pulsed electromagnetic field generator and the effect of wound healing in rats; [Darbeli elektromanyetik alan jeneratörü ile sürülen bobin tasarimlari ve siçanlarda yara iyileşme etkileri]
    (Institute of Electrical and Electronics Engineers Inc., 2017) Gumusay M.; Gulbagca F.; Saygili S.; Aydemir I.; Kaya A.; Tuglu M.I.
    Chronic wound healing is an important problem especially in diabetic and the elderly which is needed development of the current treatment methods. Electromagnetic fields (EMF) has a significant impact on our body. These fields are being used for bone problems, cartilage regeneration, and pain removal for physiotherapy purposes. Lately, EMFs are often used in in vitro and in vivo assays. In this study, in vivo test results of the applied pulsed electromagnetic fields (PEMF) with our designed Helmholtz coil array system are given. The skin wound healing conducted in rats as an experimental model provides important information for evaluating the efficacy and mechanism of these kind of treatments. For this purpose, effectiveness of PEMF compared to control groups on experimental skin healing effect was examined macroscopically. Compared with the control groups a faster wound healing process was followed by PEMF application at 75 Hz frequency. © 2016 IEEE.
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    The effect of electro magnetic fields on cell behaviour; [Elektro Manyetik Alanlarin Hücre Davranisina Etkisi]
    (Institute of Electrical and Electronics Engineers Inc., 2017) Tuglu M.I.; Gulbagca F.; Sal D.H.; Saygili S.; Sonmez P.K.; Aydemir I.; Ozkut M.; Ozturk S.; Gumusay M.; Kaya A.; Keskin N.
    The biologic effects of electomagnetic fields application on medical field has been increased. Their effect on medical treatment depends on cell behaviour which can be observed by in vivo and in vitro studies. Their effect on the stem cells and the cell lines and the organs of experimental animals with morphological alterations are helpful to understand cell behaviour. The behaviour of the cells can be analyse at ultrastructural level by scaning electron microscopy. Application of electomagnetic fields cause increase of proliferation, migration and improvement of wound healing with beneficial effects while they cause oxidative stress and cell death including apoptosis for cancer cells in different types of cell line. The minimal side effect with maximum beneficial treatment of these products suggest that they could be very useful for clinical trials. © 2016 IEEE.
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    Design of antenna-applicator for electromagnetic field applications and excitation model for rat and heat transfer analysis; [Elektromanyetik Alan Uygulamalari için Anten-Aplikatör Tasarimi, Sicąn için Uyartim Modeli ve Isi Daglimi Analizi]
    (Institute of Electrical and Electronics Engineers Inc., 2017) Gumusay M.; Gulbagca F.; Aydemir I.; Saygili S.; Kaya A.; Tuglu M.I.
    There has been only a few technical progress which contribute to wound healing in the last century. Despite these advances wound healing is very hard issue to study because of its subjectivity, diversity and complexity of the wound healing process and diversity of patients. Molecules are insufficient in the chronic wound and unlike other cells multiplying endothelial cells interrupt the healing process. To restart this process, cells can be excitated by pulsed electromagnetic fields stimulation method. When a suitable frequency and waveform electromagnetic field (EMF) signal is applied to the target tissue with the help of a coil or applicator physiological effects occurs at the cellular size. Low-frequency electromagnetic fields are non-ionizing part of the EM spectrum and they are used as adjunct therapy in soft tissue injuries. In this study, an antenna-applicator design was performed and the effect of non-thermal pulsed radio frequency energy (PRFE) system to the wound created rat skin was examined. © 2016 The Chamber of Turkish Electrical Engineers.
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    Effects of electromagnetic fields application on experimental varicocele model in rats; [Sicanlarda Deneysel Varikosel Modelinde Elektromanyetik Alan Uygulamalarinin Etki]
    (Institute of Electrical and Electronics Engineers Inc., 2017) Gulbagca F.; Sal D.H.; Saygili S.; Sonmez P.K.; Aydemir I.; Ozkut M.; Ozturk S.; Gumusay M.; Kaya A.; Tuglu M.I.
    Varicocele is the most clinical condition which can be treated by surgery. The frequency in male is 20%. The half of infertile patient has this pathology which there is dilatation in plexus pampiniformis veins of testes. Varicocele causes increase of heat or increse of oxidative stres in seminifer tubules where they produce pathology for spermatogenesis. Low frequency magnetic fields are useful for the treatment of many area where they become important role. In this study the effect of low frequency magnetic fields on the experimental varicocele model of rat testes was investigated. The parameters for oxidative stres suggest eNOS and iNOS with apoptosis were evaluated by immunohistochemical staining. There was increaese in eNOS, iNOS and apoptosis by the varicocele experiment where there was testicular damage and low frequency magnetic fields caused decrease in oxidative stress and apoptosis. Application of magnetic fields can help the clinical varicocele pathologies either with treatment or supportive effect. © 2016 IEEE.
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    In vitro cytotoxicity of guttaflow bioseal, guttaflow 2, AH-Plus and MTA fillapex
    (Iranian Association of Endodontics, 2017) Saygili G.; Saygili S.; Tuglu I.; Capar I.D.
    Introduction: The aim of the present in vitro study was to evaluate the cytotoxicity of different sealers including GuttaFlow Bioseal, GuttaFlow 2, AH-Plus and MTA Fillapex on L929 murine fibroblasts. Methods and Materials: Samples of GuttaFlow Bioseal, GuttaFlow 2, AH-Plus and MTA Fillapex were fabricated in Teflon disks of 5 mm diameter and 3 mm thickness. L929 fibroblasts were exposed to the extracts of these materials for 3, 24, 72 and 168 h at 37° C with 5% CO2. Cell viability was evaluated by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Apoptosis was evaluated by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. The data were analysed by ANOVA. Results: GuttaFlow Bioseal was nontoxic at all experimental time points (P>0.05), whereas MTA Fillapex and AH-Plus were toxic (P<0.001). At 7 days, there were more viable cells in the GuttaFlow 2 group than in the control group, and MTA Fillapex was more cytotoxic than AH-Plus. There were more apoptotic cells in the MTA Fillapex and AH-Plus groups than in the other groups at 3 h (P<0.001). Conclusion: GuttaFlow sealers are less cytotoxic than MTA Fillapex and AH-Plus. At all experimental time points, there was no significant difference in the cell viability between the GuttaFlow Bioseal group and the control group. © 2017, Iranian Association of Endodontics. All rights reserved.
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    Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study
    (Springer Science and Business Media Deutschland GmbH, 2023) Yilmaz A.; Afonso A.C.; Akil I.; Aksu B.; Alpay H.; Atmis B.; Aydog O.; Bayazıt A.K.; Bayram M.T.; Bilge I.; Bulut I.K.; Buyukkaragoz B.; Comak E.; Demir B.K.; Dincel N.; Donmez O.; Durmus M.A.; Dursun H.; Dusunsel R.; Duzova A.; Ertan P.; Gedikbasi A.; Goknar N.; Guven S.; Hacihamdioglu D.; Jankauskiene A.; Kalyoncu M.; Kavukcu S.; Kenan B.U.; Kucuk N.; Kural B.; Litwin M.; Montini G.; Morello W.; Nayir A.; Obrycki L.; Omer B.; Ozdemir E.M.; Ozkayin N.; Paripovic D.; Pehlivanoglu C.; Saygili S.; Schaefer S.; Sonmez F.; Tabel Y.; Tas N.; Tasdemir M.; Teixeira A.; Tekcan D.; Tulpar S.; Turkkan O.N.; Uysal B.; Uysalol M.; Vaiciuniene D.; Yavuz S.; Yel S.; Yildirim T.; Yildirim Z.Y.; Yildiz N.; Yuksel S.; Yurtseven E.; Schaefer F.; Topaloglu R.
    Background: The accuracy of conventional urinalysis in diagnosing urinary tract infection (UTI) in children is limited, leading to unnecessary antibiotic exposure in a large fraction of patients. Urinary heat shock protein 70 (uHSP70) is a novel marker of acute urinary tract inflammation. We explored the added value of uHSP70 in discriminating UTI from other infections and conditions confused with UTI. Methods: A total of 802 children from 37 pediatric centers in seven countries participated in the study. Patients diagnosed with UTI (n = 191), non-UTI infections (n = 178), contaminated urine samples (n = 50), asymptomatic bacteriuria (n = 26), and healthy controls (n = 75) were enrolled. Urine and serum levels of HSP70 were measured at presentation in all patients and after resolution of the infection in patients with confirmed UTI. Results: Urinary (u)HSP70 was selectively elevated in children with UTI as compared to all other conditions (p < 0.0001). uHSP70 predicted UTI with 89% sensitivity and 82% specificity (AUC = 0.934). Among the 265 patients with suspected UTI, the uHSP70 > 48 ng/mL criterion identified the 172 children with subsequently confirmed UTI with 90% sensitivity and 82% specificity (AUC = 0.862), exceeding the individual diagnostic accuracy of leukocyturia, nitrite, and leukocyte esterase positivity. uHSP70 had completely normalized by the end of antibiotic therapy in the UTI patients. Serum HSP70 was not predictive. Conclusions: Urine HSP70 is a novel non-invasive marker of UTI that improves the diagnostic accuracy of conventional urinalysis. We estimate that rapid urine HSP70 screening could spare empiric antibiotic administration in up to 80% of children with suspected UTI. Graphical abstract: A higher resolution version of the Graphical abstract is available as Supplementary information [Figure not available: see fulltext.] © 2022, The Author(s), under exclusive licence to International Pediatric Nephrology Association.
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    The relationship between urine heat shock protein 70 and congenital anomalies of the kidney and urinary tract: UTILISE study
    (Frontiers Media SA, 2023) Aksu B.; Afonso A.C.; Akil I.; Alpay H.; Atmis B.; Aydog O.; Bakkaloglu S.; Bayazıt A.K.; Bayram M.T.; Bilge I.; Bulut I.K.; Cetinkaya A.P.G.; Comak E.; Demir B.K.; Dincel N.; Donmez O.; Durmus M.A.; Dursun H.; Dusunsel R.; Duzova A.; Ertan P.; Gedikbasi A.; Goknar N.; Guven S.; Hacihamdioglu D.; Jankauskiene A.; Kalyoncu M.; Kavukcu S.; Kenan B.U.; Kucuk N.; Kural B.; Litwin M.; Montini G.; Morello W.; Obrycki L.; Omer B.; Misirli Ozdemir E.; Ozkayin N.; Paripovic D.; Pehlivanoglu C.; Saygili S.; Schaefer F.; Schaefer S.; Sonmez F.; Tabel Y.; Tas N.; Tasdemir M.; Teixeira A.; Tekcan D.; Topaloglu R.; Tulpar S.; Turkkan O.N.; Uysal B.; Uysalol M.; Vitkevic R.; Yavuz S.; Yel S.; Yildirim T.; Yildirim Z.Y.; Yildiz N.; Yuksel S.; Yurtseven E.; Yilmaz A.
    Background: Congenital anomalies of the kidney and urinary tract (CAKUT) are defined as structural malformations of the kidney and/or urinary tract. Heat shock proteins (HSPs) are expressed in the kidney in response to cellular changes, such as thermal, hemodynamic, osmotic, inflammatory, and mechanical stresses. This study aimed to assess uHSP70 levels during acute urinary tract infections (UTI) and non-infection periods in patients with CAKUT, and to evaluate whether uHSP70 is elevated in CAKUT subtypes. Methods: Among patients with CAKUT, 89 patients with UTI (CAKUT-A), 111 without UTI (CAKUT-B), and 74 healthy children were included in the study. uHSP70 levels were measured using enzyme-linked immunosorbent assay (ELISA). Results: uHSP70 level was significantly higher in the CAKUT-A group than in the CAKUT-B and healthy control groups (p < 0.0001). Moreover, the level of uHSP70 was significantly higher in the CAKUT-B group than in the control group (p < 0.0001), but was not different between the CAKUT subtypes (p > 0.05). Conclusion: Urine HSP70 can also be used to predict UTI in patients with CAKUT. Moreover, uHSP70 levels were higher in children with CAKUT during the non-infectious period than in healthy controls. This suggests that children with CAKUT are at risk of chronic non-infectious damage. Copyright © 2024 Aksu, Afonso, Akil, Alpay, Atmis, Aydog, Bakkaloglu, Bayazıt, Bayram, Bilge, Bulut, Cetinkaya, Comak, Demir, Dincel, Donmez, Durmus, Dursun, Dusunsel, Duzova, Ertan, Gedikbasi, Goknar, Guven, Hacihamdioglu, Jankauskiene, Kalyoncu, Kavukcu, Kenan, Kucuk, Kural, Litwin, Montini, Morello, Obrycki, Omer, Misirli Ozdemir, Ozkayin, Paripovic, Pehlivanoglu, Saygili, Schaefer, Schaefer, Sonmez, Tabel, Tas, Tasdemir, Teixeira, Tekcan, Topaloglu, Tulpar, Turkkan, Uysal, Uysalol, Vitkevic, Yavuz, Yel, Yildirim, Yildirim, Yildiz, Yuksel, Yurtseven and Yilmaz.
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    Pediatric kidney care experience after the 2023 Türkiye earthquake
    (Oxford University Press, 2024) Bakkaloǧlu S.A.; Delibaş A.; Döven S.S.; Taner S.; Yavuz S.; Erfidan G.; Vatansever E.D.; Aynaci F.; Yilmaz K.; Taşdemir M.; Akaci O.; Akinci N.; Güven S.; Çiçek N.; Dursun I.; Keleşoǧlu E.; Sancaktar M.; Alaygut D.; Saygili S.; Yavaşcan Ö.; Yilmaz A.; Gülleroǧlu K.; Ertan P.; Demir B.K.; Poyrazoǧlu H.; Pinarbaşi S.; Gençler A.; Baştuǧ F.; Günay N.; Çeleǧen K.; Noyan A.; Parmaksiz G.; Avci B.; Çayci F.Ş.; Bayrakçi U.; Özlü S.G.; Aksoy Ö.Y.; Yel S.; Inal G.A.; Köse S.; Bayazit A.K.; Atmiş B.; Saribaş E.; Çaǧli Ç.; Tabel Y.; Elmas A.T.; Selçuk Ş.Z.; Kiliç B.D.; Kara M.A.; Büyükçelik M.; Balat A.; Tiryaki B.D.; Erdoǧdu B.; Aksu B.; Mahmudova G.; Dursun H.; Candan C.; Göknar N.; Mutlubaş F.; Çamlar S.A.; Başaran C.; Akbulut B.B.; Düzova A.; Gülhan B.; Oruç Ç.; Peru H.; Alpay H.; Türkkan Ö.N.; Gülmez R.; Çelakil M.; Doǧan K.; Bilge I.; Pehlivanoǧlu C.; Büyükkaragöz B.; Leventoǧlu E.; Alpman N.; Zeybek C.; Tülpar S.; Gülşan R.Y.Ç.; Kara A.; Gürgöze M.K.; Önder E.N.A.; Atikel Y.Ö.; Pul S.; Sönmez F.; Yildiz G.; Akman S.; Elmaci M.; Küçük N.; Yüksel S.; Kavaz A.; Nalçacioǧlu H.; Alparslan C.; Dinçel N.; Elhan A.H.; Sever L.
    Background. Two earthquakes on 6 February 2023 destroyed 10 cities in Türkiye. We report our experience with pediatric victims during these catastrophes, with a focus on crush syndrome related-acute kidney injury (Crush-AKI) and death. Method. Web-based software was prepared. Patient demographics, time under rubble (TUR), admission laboratory data, dialysis, and kidney and overall outcomes were recorded. Results. A total of 903 injured children (median age 11.62 years) were evaluated. Mean TUR was 13 h (interquartile range 32.5, max 240 h). Thirty-one of 32 patients with a TUR of > 120 h survived. The patient who was rescued after 10 days survived. Two-thirds of the patients were given 50 mEq/L sodium bicarbonate in 0.45% sodium chloride solution on admission day. Fifty-eight percent of patients were given intravenous fluid (IVF) at a volume of 2000-3000 mL/m2 body surface area (BSA), 40% at 3000-4000 mL/m2 BSA and only 2% at > 4000 mL/m2 BSA. A total of 425 patients had surgeries, and 48 suffered from major bleeding. Amputations were recorded in 96 patients. Eighty-two and 66 patients required ventilator and inotropic support, respectively. Crush-AKI developed in 314 patients (36% of all patients). In all, 189 patients were dialyzed. Age > 15 years, creatine phosphokinase (CK) =20 950 U/L, TUR =10 h and the first-day IVF volume < 3000-4000 mL/m2 BSA were associated with Crush-AKI development. Twenty-two deaths were recorded, 20 of 22 occurring in patients with Crush-AKI and within the first 4 days of admission. All patients admitted after 7 days survived. Conclusions. These are the most extensive pediatric kidney disaster data obtained after an earthquake. Serum CK level was significantly associated with Crush-AKI at the levels of > 20 950 U/L, but not with death. Adolescent age and initial IVF of less than 3000-4000 mL/m2 BSA were also associated with Crush-AKI. Given that mildly injured victims can survive longer periods in the disaster field, we suggest uninterrupted rescue activity for at least 10 days. © The Author(s) 2024.
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    Urine soluble TLR4 levels may contribute to predict urinary tract infection in children: the UTILISE Study
    (Springer Science and Business Media Deutschland GmbH, 2024) Aksu B.; Afonso A.C.; Akil I.; Alpay H.; Atmis B.; Aydog O.; Bayazıt A.K.; Bayram M.T.; Bilge I.; Bulut I.K.; Buyukkaragoz B.; Comak E.; Demir B.K.; Dincel N.; Donmez O.; Durmus M.A.; Dursun H.; Dusunsel R.; Duzova A.; Ertan P.; Gedikbasi A.; Goknar N.; Guven S.; Hacihamdioglu D.; Jankauskiene A.; Kalyoncu M.; Kavukcu S.; Kenan B.U.; Kucuk N.; Kural B.; Litwin M.; Montini G.; Morello W.; Obrycki L.; Omer B.; Oner H.A.; Ozdemir E.M.; Ozkayin N.; Paripovic D.; Pehlivanoglu C.; Saygili S.; Schaefer F.; Schaefer S.; Sonmez F.; Tabel Y.; Tas N.; Tasdemir M.; Teixeira A.; Tekcan D.; Topaloglu R.; Tulpar S.; Turkkan O.N.; Uysal B.; Uysalol M.; Vitkevic R.; Yavuz S.; Yel S.; Yildirim T.; Yildirim Z.Y.; Yildiz N.; Yuksel S.; Yurtseven E.; Yilmaz A.
    Background: One of the most common bacterial infections in childhood is urinary tract infection (UTI). Toll-like receptors (TLRs) contribute to immune response against UTI recognizing specific pathogenic agents. Our aim was to determine whether soluble TLR4 (sTLR4), soluble TLR5 (sTLR5) and interleukin 8 (IL-8) can be used as biomarkers to diagnose UTI. We also aimed to reveal the relationship between urine Heat Shock Protein 70 (uHSP70) and those biomarkers investigated in this study. Methods: A total of 802 children from 37 centers participated in the study. The participants (n = 282) who did not meet the inclusion criteria were excluded from the study. The remaining 520 children, including 191 patients with UTI, 178 patients with non-UTI infections, 50 children with contaminated urine samples, 26 participants with asymptomatic bacteriuria and 75 healthy controls were included in the study. Urine and serum levels of sTLR4, sTLR5 and IL-8 were measured at presentation in all patients and after antibiotic treatment in patients with UTI. Results: Urine sTLR4 was higher in the UTI group than in the other groups. UTI may be predicted using 1.28 ng/mL as cut-off for urine sTLR4 with 68% sensitivity and 65% specificity (AUC = 0.682). In the UTI group, urine sTLR4 levels were significantly higher in pyelonephritis than in cystitis (p < 0.0001). Post-treatment urine sTLR4 levels in the UTI group were significantly lower than pre-treatment values (p < 0.0001). Conclusions: Urine sTLR4 may be used as a useful biomarker in predicting UTI and subsequent pyelonephritis in children with UTI. Graphical abstract: [Figure not available: see fulltext.]. © 2023, The Author(s), under exclusive licence to International Pediatric Nephrology Association.