Browsing by Author "Sen B.H."
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Item Mechanical and fluoride release properties of titanium tetrafluoride-added glass-ionomer cement(Japanese Society for Dental Materials and Devices, 2005) Pamir T.; Sen B.H.; Celik A.The aim of this study was to determine the fluoride-release and mechanical properties of a water-hardening glass-ionomer cement (GIC) (ChemFil Superior) when titanium tetrafluoride (TiF4) was added. Three experimental groups were prepared with TiF4 added to the liquid component of the material in concentrations of 0.5, 1, and 2 %. The control group was the original form of the cement and free of TiF4. After the specimens (4 mm in diameter × 6 mm in length) were prepared, their compressive strength, microhardness, modulus of elasticity, and fluoride release were measured. Data were analyzed using one-way analysis of variance (ANOVA) and post-hoc test (Bonferroni/Dunn correction). The addition of TiF4 into GIC significantly reduced fluoride release from the material with the exception of 1 % TiF4 (p< 0.0083). Compressive strengths of 0.5 and 1 % TiF4-added GICs were higher than that of the original GIC, but it was not statistically significant (p>0.05). The differences among modulus of elasticity values of experimental and control groups were not significant (p>0.05). Similarly, microhardness of GIC was not affected with TiF 4 addition (p>0.05).Item Enhanced biocompatibility of GPC by silver ion implantation(2005) Zimmerman R.; Gürhan I.; Muntele C.; Ila D.; Özdal-Kurt F.; Sen B.H.Biocompatible Glassy Polymeric Carbon (GPC) is used for artificial heart valves and in other biomedical applications. Although it is ideally suited for implants in the blood stream, tissue that normally forms around the moving parts of a GPC heart valve sometimes loses adhesion and creates embolisms downstream. Here we compare silver ion implantation and silver deposition, each of which strongly inhibits cell attachment on GPC. Inhibition of cell adhesion is a desirable improvement to current GPC cardiac implants. In vitro biocompatibility tests have been carried out with model cell lines to demonstrate that traces of silver can favorably influence the surface of GPC for biomedical applications. © 2006 Materials Research Society.Item Persistent inhibition of cell growth on silver implanted glassy polymeric carbon(Materials Research Society, 2006) Zimmerman R.L.; Gürhan I.; Ozdal-Kurt F.; Sen B.H.; Rodrigues M.; Ila D.[No abstract available]Item The influence of ion implantation on cell attachment to glassy polymeric carbon(American Institute of Physics Inc., 2006) Zimmerman R.; Gurhan I.; Ozdal-Kurt F.; Sen B.H.; Rodrigues M.; Ila D.In vitro biocompatibility tests have been carried out with model cell lines to demonstrate that near surface implantation of silver in Glassy Polymeric Carbon (GPC) can completely inhibit cell attachment on implanted areas while leaving adjacent areas unaffected. Patterned ion implantation permits precise control of tissue growth on medical applications of GPC. We have shown that silver ion implantation or argon ion assisted surface deposition of silver inhibits cell growth on GPC, a desirable improvement of current cardiac implants. © 2006 American Institute of Physics.Item Patterning of cell attachment to biocompatible glassy polymeric carbon by silver ion implantation(Materials Research Society, 2006) Zimmerman R.L.; Gurhan I.; Ila D.; Ozdal-Kurt F.; Sen B.H.; Rodrigues M.Although Glassy Polymeric Carbon (GPC) is ideally suited for implants in the blood stream, tissue that normally forms around the moving parts of a GPC heart valve. There is concern that the tissue lose adhesion and create the condition for embolisms downstream. We have shown that silver ion implantation or argon ion assisted surface deposition of silver inhibits cell growth on GPC, a desirable improvement of current cardiac implants. In vitro biocompatibility tests have been carried out with model cell lines to demonstrate that near surface implantation of silver in GPC can completely inhibit cell attachment on implanted areas while leaving adjacent areas unaffected. Patterned ion implantation permits precise control of tissue growth on medical applications of GPC. © 2006 Materials Research Society.Item The effect of osteogenic medium on the adhesion of rat bone marrow stromal cell to the hydroxyapatite(2006) Deliloglu-Gurhan I.; Tuglu I.; Vatansever H.S.; Özdal-Kurt F.; Ekren H.; Taylan M.; Sen B.H.Objective: To investigate the adhesive properties of bone marrow stromal cell (BMSC) on the hydroxyapatite (HA) particles and analyze their behavior. Methods: The study took place in the Department of the Histology and Embryology, Celal Bayar University, Manisa and in the Department of Bioengineering, Ege University, Izmir, Turkey between 2004 and 2005. We cultured BMSC from the mature rat tibia and differentiated to the osteoblasts by osteogenic medium. The BMSCs were subcultured and were taken to the HA substrate. We measured their proliferation capacity and viability with MTT assay using the spectrophotometric method. Furthermore, we identified the osteoblast-like cells by inummohistochemical staining of osteonectin and osteocalcin and we analyzed the behavior of the cells on different sized HA particles by SEM at the end of 3 days incubation. Results: Osteogenic medium caused the proliferation capacity of BMSC to speed up and the effects appeared earlier. We confirmed the osteoblastic differentiation by staining of most cells with osteoblastic markers. Subcultured cells were similarly adhesive to the HA particles and the osteogenic medium did not alter this behavior. They spread on the substrate similarly. Most of the cells demonstrated the cytoplasmic protrusion. Morphology of the cells did not change much with or without osteogenic medium. Different sizes of HA particles did not affect the adhesive properties of these cells except HA gel. The spreading and attachment ratios of the cells on HA gel were more than the others. Conclusion: We found that there was heterogeneity in BMSC on differentiation capacity to the osteoblast, which was a sign of a subpopulation. Adhesive cells showed similar morphology and behavior under the effect of osteogenic medium. The only difference was the spreading capacity on the HA gel where cell used this substrate more effectively for adhesion.Item Cell adhesion study of the titanium alloys exposed to glow discharge(2007) Abidzina V.; Deliloglu-Gürhan I.; Özdal-Kurt F.; Sen B.H.; Tereshko I.; Elkin I.; Budak S.; Muntele C.; Ila D.Titanium for biomedical application stems mainly from its advantageous bulk mechanical properties in combination with a high degree of biocompatibility that is largely attributable to their surface properties. This work is focused on the investigation of surface properties of treated titanium and cell adhesion to titanium treated in glow-discharge plasma. Pure titanium samples (grade 4) were exposed to low-energy ion irradiation in a specially constructed plasma generator, where materials were irradiated by ions of residual gases in vacuum. The ion energy was 1-10 keV. The irradiation dose was maintained at 1017 ions cm-2. The irradiation time varied from 5 to 60 min. Rutherford backscattering spectrometry (RBS) was used for surface studies. RBS showed the presence of iron on the titanium surface that occurred from the cathode of plasma generator. In vitro biocompatibility test have been carried out with model cell lines (L929 mouse fibroblasts) to demonstrate that low-energy ion irradiation can favorably influence the surface of titanium for biomedical application. Scanning electron microscopy (SEM) was the main tool to demonstrate the cell attachment properties. © 2007 Elsevier B.V. All rights reserved.Item Enhanced biocompatibility of GPC by ion implantation and deposition(2007) Zimmerman R.; Gürhan I.; Muntele C.; Ila D.; Rodrigues M.; Özdal-Kurt F.; Sen B.H.Biocompatible Glassy Polymeric Carbon (GPC) is used for artificial heart valves and in other biomedical applications. Although it is ideally suited for implants in the blood stream, tissue that normally forms around the moving parts of a GPC heart valve sometimes loses adhesion and creates embolisms downstream. We have previously shown that oxygen ion implantation slightly enhances cell adhesion to GPC. Here we compare silver ion implantation and silver deposition, each of which strongly inhibits cell attachment on GPC. Inhibition of cell adhesion is the more desirable improvement to current GPC cardiac implants. In vitro biocompatibility tests have been carried out with model cell lines to demonstrate that traces of silver can favorably influence the surface of GPC for biomedical applications. © 2007.Item Ion implantation inhibits cell attachment to glassy polymeric carbon(2007) Zimmerman R.; Deliloglu-Gurhan I.; Ozdal-Kurt F.; Sen B.H.; Rodrigues M.; Ila D.Implantation of MeV gold, oxygen, carbon ions into GPC alters the surface topography of GPC and enhances the already strong tendency for cells to attach to GPC. We have shown that implantation of silver ions near the surface strongly inhibits cell growth on GPC. Both enhanced adhesion of and inhibition of cell growth are desirable improvements on cardiac implants that have long been successfully fabricated from biocompatible glassy polymeric carbon (GPC). In vitro biocompatibility tests have been carried out with model cell lines to demonstrate that ion beam assisted deposition (IBAD) of silver, as well as silver ion bombardment, can favorably influence the surface of GPC for biomedical applications. © 2007 Elsevier B.V. All rights reserved.Item Investigation of cell growth on ion beam patterns on GPC surface(2009) Zimmerman R.; Muntele C.; Gurhan I.; Ozdal-Kurt F.; Sen B.H.; Rodrigues M.; Ila D.We have used implanted silver ions near the surface of Glassy Polymeric Carbon (GPC) to completely inhibit cell attachment and adhesion to GPC. The effect improves the safety and function of the GPC heart valve exposed to the blood stream. The strength, durability and low density make GPC a favored material for in vivo medical applications, including transcutaneous electrodes and replacement heart valves. However, the possible release of endothelial tissue that forms on the smooth surfaces of the GPC heart valve has the potential of creating an embolism. We have shown that L929 endothelial cells avoid silver implanted areas of GPC but attach and strongly adhere to areas close to silver implanted surfaces. Patterned ion implantation permits precise control of tissue growth on GPC and other biocompatible substrates. Cell growth inhibited by silver ion implanted patterns on an otherwise biocompatible substrate may be useful for in vitro studies of the way that cells sense and move away from inhospitable environments. © 2009.