Browsing by Author "Senisik, AM"

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    One-step conjugation of glycylglycine with [18F]FDG and a pilot PET imaging study
    Senisik, AM; Içhedef, Ç; Kilçar, AY; Uçar, E; Ari, K; Göksoy, D; Parlak, Y; Bilgin, BES; Teksöz, S
    This study describes a single step conjugation of Glycylglycine (GlyGly) which is a small peptide, with [F-18]FDG via oxime formation. Amiooxy-functionalization of GlyGly (AO-GlyGly) was accomplished through the reaction of Boc-aminooxy succinimide ester. Conjugation reaction was performed at 100 A degrees C for 30 min in a vial containing AO-GlyGly and [F-18]FDG solution. The radiolabeled product ([F-18]FDG-GlyGly) was obtained with 98.65 +/- 0.35% yield without any purification step which makes this method more attractive for F-18 radiolabeling. The present study is concluded with an in vivo pilot animal PET study to assess biodistribution and kinetics of chemoselectively [F-18]FDG tagged GlyGly in vivo.
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    Evaluation of New 99mTc(CO)3+ Radiolabeled Glycylglycine In Vivo
    Senisik, AM; Içhedef, C; Kilçar, AY; Uçar, E; Ari, K; Parlak, Y; Bilgin, ES; Teksöz, S
    Background: Peptide-based agents are used in molecular imaging due to their unique properties, such as rapid clearance from the circulation, high affinity and target selectivity. Many of the radiolabeled peptides have been clinically experienced with diagnostic accuracy. The aim of this study was to investigate in vivo biological behavior of [Tc-99m(CO)(3)(H2O)(3)](+) radiolabeled glycylglycine (GlyGly). Methods: Glycylglycine was radiolabeled with a high radiolabeling yield of 94.69 +/- 2%, and quality control of the radiolabeling process was performed by thin layer radiochromatography (TLRC) and High-Performance Liquid Radiochromatography (iIPLRC). Lipophilicity study for radiolabeled complex (Tc-99m(CO)(3)-Gly-Gly) was carried out using solvent extraction. The in vivo evaluation was performed by both biodistribution and SPECT imaging. Results: The high mdiolabelling yield of Tc-99m(CO)(3)-GlyGly was obtained and verified by TLRC and HPLRC as well. According to the in vivo results, SPECT images and biodistribution data are in good accordance. The excretion route from the body was both hepatobiliary and renal. Conclusion: This study shows that Tc-99m(CO)(3)-GlyGly has the potential to be used as a peptide-based imaging agent. Further studies, Tc-99m(CO)(3)-GlyGly can be performed on tumor-bearing animals.