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Browsing by Author "Sesli M."

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Olive existence in Akhisar district in Manisa Province in Turkey
(2006) Sesli M.; Tokmakoǧlu A.
In Turkey which is one of the main olive producing countries in the world, in the Aegean Region some provinces are coming forth in respect of olive existence. Since Akhisar district constitutes half of the olive existence in Manisa province it was found to be worth research. In order to detect olive existence and sector improvement in Akhisar and in selected villages a face to face survey has been held among farmers. Survey results were evaluated by the SPSS programme. At the end of the evaluation it was determined that; there was an increase in lands reserved for olive growing, olive is being preffered against tobacco which is an important agricultural product, farmers are leaning towards the Domat type whose origin is in Akhisar, intensive agricultural techniques are implemented, diseases and harmful activities are at a minimum level in olive groves. © 2006 Asian Network for Scientific Information.
RAPD-PCR analysis of cultured type olives in Turkey
(2009) Sesli M.; Yeǧenoǧlu E.D.
The aim of this study was to detect genetic similarities and distances among cultured type olive trees by RAPD-PCR technique. Olives are raised in a high range from the Aegean, Mediterranean, Marmara and Black Sea to Southeast Anatolia regions of Turkey. Olive breeding had a rapid increase in Turkey during recent years, among the agricultural products. Finding the genetic relationships between cultured type olives may help to improve genetic resources and our knowledge of their evolutionary background and to determine genetic relationships with wild type relatives. In this study, samples were obtained from the Olive Production Research Institute (Manzanilla, Domat, Gemlik and Memecik) and sapling producers in Manisa, Akhisar (Uslu, Edremit). Genomic DNA's were extracted from young leaves and PCR was used generate RAPD bands. Sixty random primers obtained from Operon Tech. were tested by RAPD-PCR (OP-A, OP-I, OP-Q). A total of 36 primers among 60 Operon random primers (Kit OP-A, OP-I, OP-Q) yielded clear and firm bands. The electrophoretic patterns of olive samples showed that 291 highly polymorphic loci. Averages of 8.08 scorable bands per primer were determined from RAPD-PCR analysis. © 2009 Academic Journals.
Genetic analysis on wild olives by using RAPD markers
(2009) Sesli M.; Yeǧenoǧlu E.D.
The aim of this study was to detect genetic similarities and distances among wild olive trees by RAPDPCR technique. Olives are raised at a high range from Artvin on the north, up to Hatay on the south and up to Mardin on the Southeast part of Turkey. The Aegean, Mediterranean, Marmara and Black Sea regions of Turkey have suitable climatic conditions for olive breeding. Olive breeding had a rapid increase in the Aegean Region during recent years, among the agricultural products. Therefore, it is required to know the genetic relationships between wild olives and their cultural relatives in order to improve genetic resources and our knowledge of their evolutionary background. In this study, samples were mainly collected from Manisa province and additionally, samples were taken from Izmir and Mugla provinces for comparison. Genomic DNA's were extracted from young leaves and PCR was used to generate RAPD bands. 60 random primers obtained from Operon Tech. were tested by RAPD-PCR. Total of 38 primers were given highly polymorphic and continuous scorable bands. 167 bands were found and each primer was screened at an average of 4.4 polymorphic bands. © 2009 Academic Journals.
Standardization of RAPD assay for genetic analysis of olive
(2009) Sesli M.; Yeǧenoǧlu E.D.
This study aimed at the standardization of PCR conditions for the purpose of determining the genetic similarities and distances in wild and cultivated olives collected from Izmir and Manisa provinces in Aegean Region. Three different PCR mix and three different PCR cycle as well as 20 primers from OP-I series were tried in 13 wild and 12 cultivated samples. A suitable PCR protocol was developed and evaluable bands were derived for further studies. Required result could not be obtained from PCR Mixes I and II and also PCR cycles I and II; however, favorable results were attained by PCR Mix III and PCR cycle III. Evaluable bands were obtained for defining the olive samples by using primers from OP-I. Thus, it was concluded that RAPD profiles are effective in the study of genetic similarities and distances of wild and cultivated olives. © 2009 Academic Journals.
RAPD assay of wild-type olives in Turkey
(Fundacao de Pesquisas Cientificas de Ribeirao Preto, 2010) Sesli M.; Yeǧenoǧlu E.D.
Genetic similarities and distances between wild-type olives in Turkey were studied using an RAPD-PCR assay. Seven wild olive tree samples were collected from villages in Manisa and Izmir provinces. Genomic DNA was extracted from young leaves and the RAPD-PCR assay was used to generate RAPD markers. Sixty-five random primers obtained from Operon Technologies were tested for the assay (OP-A 1-20, OP-I 1-20, OP-Q 1-20, and OP-J 1-5). Thirty-two of these primers yielded 115 highly polymorphic bands. The mean number of usable bands per primer for all the samples was 3.59. The genetic distance values ranged from 0.1498 to 0.6845, and genetic similarity values varied from 0.8609 to 0.5043. We found that the closest samples based on their genetic distance and similarity values were from Harlak and Sabancilar; the most distant samples were from Bornova and Bademli, © FUNPEC-RP.
Determination of the genetic relationships between wild olive (Olea europaea oleaster) varieties grown in the aegean region
(Fundacao de Pesquisas Cientificas de Ribeirao Preto, 2010) Sesli M.; Yeǧenoǧlu E.D.
The RAPD technique was used for determining genetic differences between 12 wild-olive varieties grown in the Aegean provinces of Izmir, Mugla, and Manisa in Turkey. Wild olives obtained from the same provinces were included in the same plot. Twenty of 25 operon primers (OP-I 4, OP-I 14, OP-I 15, OP-I 16, OP-I 17, OP-Q1, OP-Q2, OP-Q3, OP-Q4, OP-Q11, OP-Q12, OP-Q13, OP-Q14, OP-Q15, OP-Q16, OP-Q17, OP-Q18, OP-Q19, OP-Q20, OP-F1, OP-F2, OP-F3, OP-F6, OP-F7, OP-F8) yielded bands. The differences between the varieties were determined based on their genetic similarities, using principal coordinate analysis; genetic distances were determined using neighbor-joining analysis. The varieties wild 7 and wild 12 had the lowest genetic similarity (0.97, Jaccard similarity index); they also had the greatest genetic distance between them (0.3606, Nei's genetic distance). It was concluded that the RAPD technique is adequate for the evaluation of genetic relationships among wild olives. Principal coordinate analysis and neighbor-joining analysis gave results that support the Genetic relationships between wild olive varietie use of this type of analysis to help understand the genetic background of olives and for further genetic studies. ©FUNPEC-RP.
Application of the RAPD technique and morphological characteristics in cultivated olives (Olea europaea sativa)
(2010) Sesli M.; Yegenoglu E.D.; Gevrekci Y.
Five different morphological characteristics (width, length, width/length, stem length, stem thickness) were considered in 18 cultivated olives and discriminant analysis was applied. Using primers OP-I (1- 20) and OP-Z (7, 8, 9, 10 and 11) in the same olives, evaluable bands were obtained from 12 primers out of 25. PCR analysis was repeated twice and those providing stable bands in the end of both analyses were evaluated. Genetic distances of cultivated olives were determined using neighbor-joining (Mega 4.1 program) method. SPSS 15.0 package program was used in the evaluation of morphological data of plants with the discriminant analysis; and in accordance with the discriminant results, while accurate estimations were made among the groups in olives, there had been misclassified estimations in some of them. The purpose of study is to determine the genetic distances of cultivated olives which economically important by applying Neighbor-joining method with the data obtained using RAPD analysis; and to classify the some morphological characteristics of same olives with the discriminant analysis. ©2010 Academic Journals.
Resistance of olive cultivars to verticillium dahliae
(2010) Sesli M.; Onan E.; Oden S.; Yener H.; Yegenoglu E.D.
Seventeen important olive cultivars have been evaluated for resistance to Verticillium dahliae under controlled conditions. One-year-old nursery olive plants were inoculated with cotton defoliating (D) (Mn 16) isolate (VCG1) of V. dahliae. Resistance was evaluated by assessing symptom severity using a 0 - 4 rating scale and estimating the area under disease progress curves. Besides, additional parameters for including cultivars into a defined category were used such as the percentage of plants killed and those which recovered from the disease. All cultivars were susceptible to isolate of V. dahliae at different levels. 'Wild 6' and 'Gemlik 2' were moderately susceptible to isolate of V. dahliae. Their resistance was evident by the plant ability to recover from infection with isolate. © 2010 Academic Journals.
Compare various combinations of similarity coefficients and clustering methods for Olea europaea sativa
(2010) Sesli M.; Yegenoglu E.D.
The aim of the study was to compared the genetic similarity coefficients (Jaccard, Dice, Simple Matching) and different clustering methods (UPGMA, WPGMA, Single Linkage and Complete Linkage) combinations for cultivated olives. A total of 12 samples, Gemlik, Manzanilla, Edremit, Domat, Uslu and Memecik cultivars were screened with RAPD-PCR analysis by Operon random primers OP-Q kit. The closest samples based on their genetic similarity values have been found as 'Edremit and Gemlik 5' and the most distant ones have been found as 'Manzanilla 1 and Gemlik 3' in both of Dice and Jaccard coefficients; whereas, in simple-matching coefficient, the closest samples based on their genetic similarities have been determined as Edremit and Gemlik 5 and the most distant ones have been determined as Manzanilla 1 and Gemlik 3 and also Manzanilla 1 and Gemlik 4. The results from Mantel test of original matrices show that the correlation between Jaccard and Dice similarity matrices was high and significant (0.9971). UPGMA clustering for Dice coefficient was given a highest cophenetic correlation as 0.9571 and Complete linkage clustering for Simple Matching was yielded a lowest correlation value as 0.8992. The results obtained from consensus indices shown that Consensus fork index was found (CIC =0, 9000) in Jaccard and Dice coefficients. Simple Matching coefficient had very low values with the Dice and Jaccard coefficients (CIC =0.1000). PCO analysis provided results matching up one-to-one with the data obtained from Dice and Jaccard coefficient UPGMAs. © 2010 Academic Journals.
Genetic dissimilarities between wild olives by random amplified polymorphic DNA (RAPD) assay
(2010) Sesli M.; Yegenoglu E.D.
Olive, being a fruit of subtropical climate and recognized to have significant importance in terms of human health, is being investigated with different aspects. Wild olives are valuable in the sense that they contain the sources of stability and they can be separated by using random amplified polymorphic DNA (RAPD) technique. In this study, a total of 59 primers were used for the purpose of determining the DNA fingerprints of 12 wild olives obtained from different provinces of Aegean region, and 92 polymorphic bands were yielded. Mean number of polymorphic bands per individual was calculated as 7.67 and number of polymorphic bands per scorable primer was calculated as 4.6. Specific DNA markers are important for determining the genetic relation among wild subspecies in olives. The purpose of this study is to identify the wild olives by using RAPD analysis. In conclusion, the genetic relation between wild olives were determined through Jaccard, Sorensen Dice, Simple Matching coefficients, (unweighted pair group method with arithmetic averages) UPGMA and neighbor joining method by using the data obtained with RAPD. © 2010 Academic Journals.
Comparison of similarity coefficients used for cluster analysis based on RAPD markers in wild olives.
(2010) Sesli M.; Yegenoglu E.D.
Five different similarity coefficients (Jaccard, Sorensen-Dice, simple matching, Rogers and Tanimoto, and Russel and Rao) were evaluated and 10 wild olives analyzed with RAPD markers. The influence of the similarity coefficients on wild olives clustering was investigated. Forty-five primers were used on samples from 10 wild olives (Wild 1 and 2 obtained from Mugla province; Wild 3, 4, 5, 6, 7, and 8 from Manisa province and Wild 9 and 10 from Izmir province of Turkey). The similarity matrices obtained from RAPD markers were compared by the Mantel test. Cluster analysis was made with UPGMA dendrograms, and the consensus fork indexes between all pairs of dendrograms were calculated. The Jaccard and Sorensen-Dice coefficients gave the same results, due to the fact that both exclude negative co-occurrences. The dendrograms using the simple matching and Rogers and Tanimoto coefficients were similar; Wild 4 (Akhisar, Manisa) and Wild 9 (Bornova, Izmir) olives had the closest genetic similarities. This occurred because these coefficients include negative co-occurrences. The Russel and Rao coefficients produced different results, because they include negative co-occurrences in the denominator. We concluded that the coefficients that do not include negative co-occurrences are more efficient for studies of wild olives clustering based on RAPD markers.
Genetic relationships among and within wild and cultivated olives based on RAPDs.
(2010) Sesli M.; Yeǧenoǧlu E.D.
We examined genetic relationships among wild and cultivated olives, which is a very important crop in the economy of the Aegean region. We used RAPD analysis to evaluate relationships among and within 22 olive subspecies from Manisa, Mugla and Izmir provinces in Turkey. Twelve of the subspecies were wild and 10 were cultivated olives. Fifty-two primers were used (OP-Q 1-20, OP-I 1-20, OP-F 14-15-16-17, and OP-K 1-8) and 49 polymorphic bands were selected and used for analysis. The dendrogram based on unweighted pair-group cluster analysis using the Sorensen-Dice coefficient of similarity index indicated two major groups, dividing wild olives from cultivated olives. The patterns of genetic relationships among and within the different olives were analyzed by means of analysis of molecular variance. We found significant differences between wild and cultivated olives (Phi(st) = 0.1507; P < 0.001). In order to determine the genetic relationship among wild and cultivated olives, principal coordinate analysis was used to examine the variation among subspecies. The wild and cultivated olives formed two main groups, one on the right side and the other on the left side of the principal coordinates graph, respectively. This was compatible with the results we obtained from analysis of molecular variance.
Comparison of Manzanilla and wild type olives by RAPD-PCR analysis
(Academic Journals, 2010) Sesli M.; Yeǧenoǧlu E.D.
The object of this study was to detect genetic similarities and distances between cultivated Manzanilla and wild type olives by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique. In this study, the cultivated type olive Manzanilla was supplied from Olive Research Institute of Turkey and the wild type olives from the villages of Manisa, Izmir and Mugla provinces. Genomic DNA's were extracted from young leaves and PCR was used to generate RAPD bands. Sixty random primers obtained from Operon technology were tested by RAPD-PCR (OP-A, OP-I, OP-Q). Forty one of 60 primers used in the study provided 346 considerably polymorphic bands and the average number of bands was defined as 8.43. Comparisons of cultivated and wild type olives are important for understanding genetic relation. It was determined that the genetic distance values varied between 0.0665 and 0.2863 and genetic similarity values varied between 0.9356 and 0.7511. It was observed that the samples most close to each other were wild 8 and wild 3 and the samples most distant from each other were wild 5 and Manzanilla, in accordance with such genetic distance and similarity values. © 2010 Academic Journals.
Genetic relationships in wild olives (Olea europaea ssp. oleaster) by ISSR and RAPD markers
(Taylor and Francis Ltd., 2017) Sesli M.; Yegenoglu E.D.
Wild olives or oleaster (Olea europaea ssp. oleaster) are found naturally in the Mediterranean basin. The changes in climate and environmental conditions have been gradually accelerating in the last decades. Since oleasters are more resistant to diseases, pests and unfavorable environmental conditions, they have become a very important source for resistance breeding studies that would be required in the future. Twelve inter simple sequence repeat (ISSR) and 12 random amplified polymorphic DNA (RAPD) markers were used; the effectiveness of these markers was studied and different parameters such as effective multiplex ratio, marker index, resolving power, and polymorphic information content, Shannon information index (I), effective allele number (ne), and Nei's genetic distance (h) were used in the determination of genetic varieties of 18 oleasters grown in Turkey. When both marker systems were analyzed in this study, it was observed that MI, RP and EMR parameters in ISSR gave higher values than RAPD while ne, I, and h were lower in ISSRs. Neighbour-joining dendrograms were developed by using Nei's genetic distance matrix, and principal coordinate analysis was performed. © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
Determining the genetic relationships between cultivated type olives using ISSR and morphological markers
(Triveni Enterprises, 2017) Yegenoglu E.D.; Sesli M.; Gevrekci Y.
Aim: The study aimed to compare morphological characters and Inter-simple sequence repeat data based trees, and examine the genetic relations in ten olive varieties among cultivated type olives grown commonly in different regions of Turkey. Methodology: Ten olive varieties were evaluated with some morphologic markers and ISSR marker. All analyses were conducted with Numerical Taxonomy System (NTSYS). The cluster analysis was performed with unweighted pair group method with arithmetic average (UPGMA) clustering algorithm. Results: The results showed that there was a moderate correlation between pairwise distances estimated from ISSR data and distances from morphological characters (0.511). The Euclidean Distance matrix represented that the lowest value was between Tavsan Yuregi and Cilli (1.62), while the highest value was between Manzanilla and Cekiste (7.91). According to Jaccard coefficient, the samples closest to each other were (Memecik and Gemlik); and the samples farthest to each other were (Halhali and Manzanilla). I n t e r p r e t a t i o n: Determining the genetic relations in agriculturally economic plants is valuable in terms of protecting the gene sources, determining t h e h o m o n y m s a n d synonyms, and developing b r e e d i n g p r o g r a m s. M o r p h o l o g i c a l a n d molecular markers may be used in the identification of genetic variability. Mutually complementary information can be obtained by using m o r p h o l o g i c a l a n d molecular markers together. © Triveni Enterprises, Lucknow (India).
Assessment of genetic relations among cultivated olives by ISSR markers
(Tarbiat Modares University, 2017) Dilsat Yegenoglu E.; Sesli M.
Olive grows in the mid-latitudes in the world and in areas with Mediterranean climate. Olive is one of the important agricultural products in Mediterranean Basin and Turkey. Its contribution to economy makes it valuable to determine the genetic relations among different olive varieties. In this study, genetic variations among 13 different olive cultivars were determined through 22 ISSR primers by obtaining valuable bands from 7 of them. The cultivars were mainly Turkish, namely, Edremit, Gemlik, Domat, Uslu, Çilli, Eşek, Kaba, Çekişte Nazilli, Memecik, Tavşan Yüreği, Halhali, Manzanilla, and Çekişte Bozdoğan, grown in different regions. Totally, 92 bands were obtained and it was observed that all of these were polymorphic. Polymorphic Information Content (PIC), Marker Index (MI), and Resolving Power (RP) averaged 0.19, 2.36 and 2.73 per primer, respectively, showing the high efficiency and reliability of the markers used. In conclusion to ISSR markers analyses as the number of polymorphic loci, genetic diversity and olive relationships through UPGMA and Neighbor Joining dendrogram based on Nei’s genetic distance and PCO, it was observed that Tavşan Yüreği and Halhali varieties formed one set and Çekişte Nazilli and Manzanilla varieties formed another set together. © 2017, Tarbiat Modares University. All rights reserved.
UPGMA and artificial neural networks applications on wild type olives
(Triveni Enterprises, 2017) Sesli M.; Yegenoglu E.D.; Altintas V.; Gevrekçi Y.
Aim: Plant genetic sources are important to study genetic variability and richness of hereditary knowledge of plant species in gene pool. Local varieties, rural populations, wild types and old varieties are the primary ones. In this respect, wild type olives (Olea europaea oleaster) are valuable in terms of olive breeding, cultivation and ecosystem. The aim of the study was to determine genetic distances between olive varieties. Methodology: Artificial Neural Networks intuitive algorithm application was performed on seven wild type olives grown in different regions of Turkey by using data obtained from twenty-two ISSR primers. Results: UPGMA dendrograms were developed through Jaccard, simple matching coefficients, and similarity matrices; and genetic similarities and dissimilarities were exhibited. Interpretation: It was concluded that Artificial Neural Networks would be beneficial for estimating olive types accurately based on the results obtained from earlier studies performed with genetic markers. © Triveni Enterprises, Lucknow (India).
A fuzzy logic application to predict egg production on laying hens; [Yumurtacı tavuklarda yumurta veriminin tahminlenmesinde bulanık mantık uygulaması]
(Veteriner Fakultesi Dergisi, 2019) Gevrekçi Y.; Altintaş V.; Yeğenoğlu E.D.; Takma Ç.; Atil H.; Sesli M.
Fuzzy logic has a great potential for researchers and it has been developed over the last two decades. In animal science, there are limited numbers of studies on fuzzy logic approach. This study was carried out to examine the Fuzzy logic applications for prediction of the egg production data. Egg production records were obtained from the commercial poultry farm in Izmir, Turkey. Egg production traits of brown laying hens at 22 to 40 weeks of age were analyzed with Fuzzy logic system. In this study, Fuzzy logic model was developed for the prediction of egg production values of three classes; top, middle and lower (bottom for the cage effect) production. For this purpose 120 data lines representing 4 inputs consisting of cage, age at sexual maturity (ASM), body weight at sexual maturity (BWSM), body weight at mature age (BW) and 1 output, egg production (EP) that collected daily and individually were used in a Fuzzy logic model. The similarities between predicted and original production records were investigated, the coefficient of determination (R 2 ) was found as 0.89 which was also shown the prediction’s success rate. The probability of egg production at ASM of 168 days, BWSM of 1500 g and BW of 1820 g was found 98.97% while egg production’s probability at ASM of 157 days, BWSM of 1720 g and BW of 1940 g was determined as 97.97%. These results were also concluded that layers reached at sexual maturity later have lower egg production. The results illustrated that Fuzzy model could provide an effective and accurate prediction for classifying egg production of laying hens. However, since the applications of fuzzy logic related to the prediction of egg production are limited, this work will be pioneered by future studies. © 2019, Veteriner Fakultesi Dergisi. All rights reserved.
Determination of olive cultivars by deep learning and ISSR markers
(Triveni Enterprises, 2020) Sesli M.; Yegenoglu E.D.; Altintas V.
Aim: The aim of the study was to make accurate estimation of olive varieties by using morphologic characters through deep learning and genetic characters through ISSR (Inter Simple Sequence Repeats) markers. Methodology: In this study, 800 leaf samples were collected from olive varieties and training and testing was performed; 600 samples were assessed for the training process and 200 samples were assessed for the testing process. Convolution of neural networks is a component of deep learning which is used frequently in image processing was used in this study. Results: Based on the results of such classification, the designed model was successful at a rate of 89.57% and it was also determined that this structure can be used in the area of problem. Interpretation: The success of convolution neural networks in terms of classification was exhibited. In ISSR method, the evaluation was performed on the basis of DNAs, i.e., genetic properties of varieties by means of ISSR markers. © Triveni Enterprises, Lucknow (India).

Browsing by Author "Sesli M."

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