Browsing by Author "Sidal, U"

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    Investigation of Poly-β-Hydroxybutyrate (PHB) Production by Bacillus subtilis ATCC 6633 Under Different Conditions
    Tamdogan, N; Sidal, U
    The aim of our study was to assess the PHB productivity of Bacillus subtilis ATCC 6633 strain under different conditions. PHB amount was measured by UV-VIS spectrophotometer at 235 nm. The effects of the incubation time, temperature, pH, carbon sources, nitrogen sources and the rate of carbon/nitrogen ratio on PHB production rate were investigated. The results demonstrated that the highest PHB production was obtained after 24 h of incubation (10.4981 mu g/ml), at 7.0 of pH (10.4981 mu g/ml), with D-mannitole (23.6623 mu g/ml) as carbon source, L-glycine (14.6217 mu g/ml) as nitrogen source, C/N ratio of 2.5 (3.2481 mu g/ml) and with a temperature of 30 degrees C in culture media (10.4981 mu g/ml).
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    A Native Mixed Plasmodium falciparum and Plasmodium vivax Malaria Case Molecularly Proven After 22 Years in Manisa, Turkey
    Ok, ÜZ; Çavus, I; Sidal, U; Lmoncu, E; Özbilgin, A
    Plasmodium falciparum malaria causes about 450.000 deaths every year, mostly in children around the world. The infection is seen in cases coming from abroad and may lead to deaths in Turkey. Many native P.falciparum malaria cases and deaths due to this infection were observed in Turkey during mid 1900's when malaria was epidemic. But only two native cases were reported in the last 50 years, both from Manisa. First case was a one-year old baby who has come to Manisa from Urfa with his family and has never been abroad. He has diagnosed with Plasmodium vivax malaria and treated with chloroquine and primaquine. A previously obtained thin blood film was examined and characteristic P.falciparum rings in red blood cells were observed and the case was published together with photographs as probable P.falciparum and P.vivax mixed infection. After this case, microscopists working in Malaria Control Unit of Manisa were informed about the differentiation of malaria species in thin blood samples. Soon afterward, another case who have never been abroad before were also diagnosed with P.falciparum and P.vivax mixed infection and this case was also published with photographs taken from thin blood samples. As molecular diagnostic methods were not improved and widespread in those years, it could not be applied in both cases. A Giemsa stained thin blood sample of the baby case was incidentally found 22 years afterwards and with the aim of molecular diagnosis, the blood sample on the slide previously processed for DNA isolation, then analysed with FTD Malaria Differentiation (Fast Track Diagnostics, Luxembourg) multiplex kit with real-time polymerase chain reaction by using probes special for P.falciparum, P.ovale, P.malariae, P.vivax species. DNA's belonging to P.falciparum and P.vivax were found to be positive, the case is molecularly proved to have P.falciparum and P.vivax mixed infection. This case indicated that Turkey is convenient for the expansion of P.falciparum malaria in terms of the climate and vectors and suggested that the potential danger may increase with the effects of global warming, wars and migrations and may jump to Europe over Turkey. The case which molecularly proved the existence of native P.falciparum malaria in the near future in Turkey, was presented to draw attention to the danger of this infection for Turkey and Europe.
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    Rhamnolipid production from olive oil mill effluent (OOME) using the rotating biodisc reactor
    Sidal, U; Özkale-Taskin, E
    This paper presents a new process for rhamnolipid production from olive oil mill effluent (OOME) by a bacterial strain Pseudomonas sp. A01 isolated from olive oil mill contaminated soil. A biodisc reactor traditionally exploited for waste treatment was used for the production of rhamnolipid. The employed medium contained 10% OOME plus NaNO(3). After optimal physiological conditions were reached using batch culture techniques, they were applied for biodisc reactor. The yield and rhamnolipid production determined at 72 hours of incubation in the biodisc reactor were 0.69 x 10(-9) g/total cell and 0.115 g/L, respectively.
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    Investigation of antimicrobial effects of a Pseudomonas-originated biosurfactant
    Yilmaz, ES; Sidal, U
    The aim of this work was to investigate the antimicrobial effects of biosurfactant (rhamnolipid) produced from Pseudomonas sp. Eight clinical test microorganisms were chosen, which were different groups, for the antimicrobial assays. Antimicrobial activity was evaluated according to the minimal inhibitor concentration (MIC) and disc-diffusion method. The highest activity for the rhamnolipid discs was obtained for beta-hemolytic Streptococcus sp., whereas the lowest activity was found for Pseudomonas aeruginosa. The biosurfactant (rhamnolipid) showed very strong antimicrobial activity against the microorganisms tested.
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    Production of Rhamnolipid (A Biosurfactant) Using Free and Immobilized Cells of Pseudomonas sp.
    Sidal, U; Yilmaz, ES
    This study presents a method for the production of rhamnolipid, a biosurfactant, by Pseudomonas sp. Pseudomonas sp. cells that were grown in nutrient agar were inoculated into sterile liquid medium. Following an incubation period of 24 h, 2 ml of cells were inoculated into a different liquid medium and the results were obtained at the end of 26 hours incubation time. In our study, the effects of temperature, pH, and glucose concentration on rhamnolipid production were also investigated. Later, the same procedure was applied to immobilized cells that were kept away from the free microorganisms. The production of rhamnolipid by free cells was found to be much higher than that of immobilized cells. Free cells could be used for rhamnolipid production effectively.