Browsing by Author "Sivrikoz, ON"

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    Prophylactic vitamin D supplementation in ovarian hyperstimulation syndrome: an animal study (vol 292, pg 421, 2015)
    Turan, GA; Eskicioglu, F; Sivrikoz, ON; Cengiz, H; Gur, EB; Tatar, S; Sahin, N; Yilmaz, O
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    Prophylactic vitamin D supplementation in ovarian hyperstimulation syndrome: an animal study
    Turan, GA; Eskicioglu, F; Sivrikoz, ON; Cengiz, H; Gur, EB; Tatar, S; Sahin, N; Yilmaz, O
    To investigate the effect of vitamin D in ovarian hyperstimulation syndrome (OHSS). In this animal study, 28 immature female Wistar rats were divided into four groups: group 1 (control); group 2 (ovarian stimulation); group 3 (OHSS group); group 4 (OHSS + vitamin D group). All groups were killed 48 h after hCG administration and were compared in terms of vascular permeability, ovarian weight, ovarian diameter, vascular endothelial growth factor (VEGF) expression (immunohistochemistry) in ovarian tissue and pigment epithelium-derived factor (PEDF) level in the serum (ELISA test) with the Kruskal-Wallis and Mann-Whitney U tests. VEGF expression in the vitamin D group was similar to that in the OHSS group. However, the PEDF level was significantly higher in the vitamin D group (p = 0.013). Prophylactic vitamin D supplementation is not sufficiently effective in preventing OHSS. Vitamin D effectively increases PEDF, which has an opposing effect on VEGF, which plays a key role in OHSS. Thus, the protective effect of Vitamin D on OHSS should be investigated with a vitamin D deficient model in the study group.
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    Myo-inositol is a promising treatment for the prevention of ovarian hyperstimulation syndrome (OHSS): an animal study (vol 292, pg 1163, 2015)
    Turan, GA; Eskicioglu, F; Sivrikoz, ON; Cengiz, H; Adakan, S; Gur, EB; Tatar, S; Sahin, N; Yilmaz, O
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    The role of stem/progenitor cells and Wnt/β-catenin signaling pathway in the patients with prostate cancer
    Vatansever, HS; Gumus, B; Aydogdu, O; Sivrikoz, ON; Türköz-Uluer, E; Kivanç, M; Atesçi, YZ; Bugdayci, H
    Aim. The aim of this paper was to investigate the possible effect of cancer stem cells (CSCs) and relationship with Wnt/beta-catenin signaling pathway progressing of prostate cancer. Methods. Thirty men with a pathological diagnosis of benign prostate hyperplasia (BPH) (group 1, N.=10), prostate cancer with a gleason score of <= 6 (group 2, N.=10), and prostate cancer with a gleason score of >6 (group 3, N.=10) were included in the study. The patients' groups were compared in terms of immunoreactivity strength of prostatic stem/progenitor cell surface markers including CD133 and CD117. We also compared the immunoreactivity of Wnt7a, a part of Wnt signaling pathway which has a potential role in the progression of several cancers including prostate cancer. The immunoreactivity of Frizzled 6 (Fzd 6) which is the receptor of Wnt family was also evaluated in all groups. Results. Immunohistochemical analyses demonstrated that although CD133 immunoreactivity was positive in all groups, immunoreactivity was significantly stronger in group 3 when compared to other groups. While CD 117 irnmunoreactivity was negative in group 1 and 2, it was positive in group 3. Wnt7a immunoreactivity was weak in all groups and Fzd 6 immunoreactivity was stronger in group 1 and 3 when compared to group 2. Conclusion. Our findings demonstrated that CSCs and Wnt signaling pathway have a potential role in the development and progression of prostate cancer.
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    Montelukast is effective in preventing of ovarian hyperstimulation syndrome; an experimental study
    Eskicioglu, F; Turan, GA; Sivrikoz, ON; Cengiz, H; Akan, Z; Sahin, N; Yilmaz, O; Yesil, H; Vatansever, S
    Objectives: To determine the efficacy of montelukast in comparison with cabergoline in the prevention of ovarian hyperstimulation syndrome (OHSS) in rats. Material and methods: An experimental OHSS model was formed in 35 female Wistar rats. Rats (22 days old) were randomized into 5 groups, each containing 7 animals. The control group received no therapy; the mild OHSS group was administered pregnant mare serum gonadotropin (PMSG) 10 IU for 4 days, hCG 10 IU on the 5th day; the severe OHSS group received PMSG 10 IU for 4 days, hCG 30 IU on the 5th day. The montelukast group: received montelukast 10 mg/kg/day and the cabergoline group was administered cabergoline 100 mu g/kg/day via oral gavage for 6 days (days 22-27), in addition to those of severe OHSS. All groups were sacrificed on 28th day. Body weight, ovarian diameter and weight, vascular permeability, vascular endothelial growth factor (VEGF), semiquantitative VEGF receptor-1, and VEGF receptor-2 (VEGFR-2) immunohistochemistry were evaluated. Results: Ovarian diameter and VEGF expression were significantly lower in the montelukast and cabergoline groups than in the severe OHSS group. While montelukast was more effective in limiting vascular permeability in the severe OHSS, cabergoline was superior to montelukast with respect to the limiting effect on increased body weight and VEGFR-2 expression. Conclusions: The VEGF/VEGFR-2 interaction plays an important role in OHSS pathogenesis. Montelukast limits VEGF expression, and cabergoline reduces both VEGF and VEGFR-2 expressions; they are both effective therapies for the prevention of severe OHSS.
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    The Role of Transforming Growth Factor Beta and Smad Receptors in Determining Prognosis in High-Grade Primary Brain Tumors: Glioblastoma Multiforme
    Gürsoy, G; Barutçuoglu, M; Sivrikoz, ON; Gökalp, S; Vatansever, S
    Introduction High-grade primary brain tumors cause serious morbidity and mortality. This study aimed to investigate the role of transforming growth factor beta (TGF-beta) and suppressor of mothers against decapentaplegic (Smad) receptors in high-grade primary brain tumors. \Material and Method Thirteen patients with a pathological diagnosis of glioblastoma multiforme were included in the study. Pathological preparations of each patient were analyzed retrospectively in histochemistry and immunohistochemistry laboratories. Transforming growth factor beta 1, TGF-beta 2, TGF-beta 3, Smad 1/2/3, Smad 6, and Smad 7 stainings were evaluated, and the immunoreactivity densities were examined. Result We found out an increase in the expression of TGF-beta 1 and TGF-beta 3 protein. Regarding the inhibitin receptors, Smad 6 showed much more expression than Smad 7. Thus, we found that Smad 6 has a protective effect and role in the tissue. Immunhistochemically, TGF-beta family stains, which are activated by types I-and -II receptors, and the stainless staining of the Smad family might also be showing that the TGF-beta family is taking action with a secondary pathway other than the Smad family. Conclusion In addition to Smad family receptors, Shc-GBR2, SARA, and Ras-Erk1/2 receptors should be investigated in future research. After that, the prognosis, diagnosis, and patient-based chemotherapy strategies for the treatment of glioblastoma multiforme may take a more prominent role.