Browsing by Author "Sonmez P.K."

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    The effect of electro magnetic fields on cell behaviour; [Elektro Manyetik Alanlarin Hücre Davranisina Etkisi]
    (Institute of Electrical and Electronics Engineers Inc., 2017) Tuglu M.I.; Gulbagca F.; Sal D.H.; Saygili S.; Sonmez P.K.; Aydemir I.; Ozkut M.; Ozturk S.; Gumusay M.; Kaya A.; Keskin N.
    The biologic effects of electomagnetic fields application on medical field has been increased. Their effect on medical treatment depends on cell behaviour which can be observed by in vivo and in vitro studies. Their effect on the stem cells and the cell lines and the organs of experimental animals with morphological alterations are helpful to understand cell behaviour. The behaviour of the cells can be analyse at ultrastructural level by scaning electron microscopy. Application of electomagnetic fields cause increase of proliferation, migration and improvement of wound healing with beneficial effects while they cause oxidative stress and cell death including apoptosis for cancer cells in different types of cell line. The minimal side effect with maximum beneficial treatment of these products suggest that they could be very useful for clinical trials. © 2016 IEEE.
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    Effects of electromagnetic fields application on experimental varicocele model in rats; [Sicanlarda Deneysel Varikosel Modelinde Elektromanyetik Alan Uygulamalarinin Etki]
    (Institute of Electrical and Electronics Engineers Inc., 2017) Gulbagca F.; Sal D.H.; Saygili S.; Sonmez P.K.; Aydemir I.; Ozkut M.; Ozturk S.; Gumusay M.; Kaya A.; Tuglu M.I.
    Varicocele is the most clinical condition which can be treated by surgery. The frequency in male is 20%. The half of infertile patient has this pathology which there is dilatation in plexus pampiniformis veins of testes. Varicocele causes increase of heat or increse of oxidative stres in seminifer tubules where they produce pathology for spermatogenesis. Low frequency magnetic fields are useful for the treatment of many area where they become important role. In this study the effect of low frequency magnetic fields on the experimental varicocele model of rat testes was investigated. The parameters for oxidative stres suggest eNOS and iNOS with apoptosis were evaluated by immunohistochemical staining. There was increaese in eNOS, iNOS and apoptosis by the varicocele experiment where there was testicular damage and low frequency magnetic fields caused decrease in oxidative stress and apoptosis. Application of magnetic fields can help the clinical varicocele pathologies either with treatment or supportive effect. © 2016 IEEE.
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    Biocompatibility of vertically aligned multi-walled carbon nanotube scaffolds for human breast cancer cell line MDA-MB-231
    (SpringerOpen, 2017) Akinoglu E.M.; Ozbilgin K.; Sonmez P.K.; Ozkut M.M.; Giersig M.; Inan S.; Gumustepe E.; Kurtman C.
    The aim of the current study was to determine whether the MWCNT-based scaffold has a suitable structure for cell growth and provides a biocompatible environment for human MDA-MB-231 cell lines. MWCNT-based nanostructured scaffolds were produced by plasma-enhanced chemical vapor deposition (PECVD) technique. MDA-MB-231 cells were seeded on MWCNTs-textured silicon scaffolds and on pristine silicon surfaces. After 1 week of culturing, the scaffolds were prepared for SEM analysis and immunocytochemical staining was performed for the two groups (MWCNT scaffold and pristine silicon surface), using MMP-2, MMP-9, PI3K, AKT and NF-κB primary antibodies. SEM analyses showed that the MDA-MB-231 cells better adhered to the MWCNT-based nanostructured scaffold than the pristine silicon surface. Immunohistochemical activity of the MDA-MB-231 cells on both materials has similar staining with anti-AKT MMP-2, MMP-9 and NF-κB primary antibodies. Therefore, the results of the present study suggest that the MWCNT-based scaffolds enhanced cell adhesion to the scaffold and exhibited more biomimetic properties and physiological adaptation with the potential to be used for in vitro metastasis studies of BrCa cell lines. © 2017, The Author(s).
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    Do Wortmannin and Thalidomide induce apoptosis by autophagy inhibition in 4T1 breast cancer cells in vitro and in vivo?
    (E-Century Publishing Corporation, 2021) Uluer E.T.; Sonmez P.K.; Akogullari D.; Onal M.; Tanriover G.; Inan S.
    The aim of this study was to show the effects of autophagy inhibitor Wortmannin and antiangiogenic-proapoptotic Thalidomide on autophagy and apoptosis markers in 4T1 breast cancer cells in vitro and in vivo. The half-maximal inhibitory concentration (IC50) values of 4T1 cells for Wortmannin and Thalidomide were evaluated by Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. After cancer formation in 28 BALB/C female mice, drugs were administered for seven days. Cells and tissue sections were evaluated for anti-phosphoinositide 3-kinase (PI3K), anti- the microtubule-associated protein 1 light chain3 (MAPLC3β), anti-caspase 8, anti-caspase 9, and anti-caspase 3 immunoreactivities by immunohistochemical staining and apoptosis by Terminal Transferase dUTP Nick End Labeling (TUNEL) assay. Both PI3K and MAPLC3β immunoreactivities decreased in all treatments when compared to control group except Thalidomide treatment in primary cancer tissue. The caspase 3, 8, and 9 immunoreactivities were increased in all treatment groups and TUNEL positive cells were the highest in the Wortmannin and Thalidomide group. Our findings suggest that autophagy is an important mechanism for 4T1 cells and both Wortmannin and Thalidomide treatments inhibit autophagy and induce apoptosis. In primary cancer tissues, autophagy was not effective as in vitro. The treatment of Wortmannin and Thalidomide increased the apoptotic cells in vivo independent from autophagy inhibition. Different results may be because of microenvironment. Further studies must be done to elucidate the effect of microenvironment. © 2021 E-Century Publishing Corporation. All rights reserved.
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    In-Vitro Evaluation of Immunomodulation Effects of Mesenchymal Stem Cell-Derived Exosomes in Refractory Chronic Spontaneous Urticaria
    (Bilimsel Tip Yayinevi, 2023) Ozdemir A.T.; Kirmaz C.; Ozdemir R.B.O.; Oztatlici M.; Sonmez P.K.; Tuglu M.I.
    Objective: Approximately half of chronic spontaneous urticaria (CSU) patients are thought to have an autoimmune pathology, and they are resistant to current treatment approaches. Mesenchymal stem cells (MSCs) are adult cells that have been shown to be useful in many autoimmune pathologies due to their immunomodulation properties. This study aimed to investigate the immunomodulatory effects of MSCs, and exosomes isolated from refractory CSU patients. Materials and Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from 5 refractory CSU patients and 5 healthy volunteers. The effects of MSCs isolated from CSU patients and healthy MSCs were compared. Co-culture experiments were performed to evaluate the efficacy of Mesenchymal Stem Cells and exosomes on PBMCs of CSU patients and healthy volunteers. To compare the resulting effects, changes in IFN-γ, IL-4, IL-10, IL-17a, and TGF-β cytokines were detected by the ELISA method. Cell proliferations were detected with the CCK-8 kit. Results: The effects of autologous and allogeneic MSCs on IFN- γ expressions were similar, both providing significant suppression at all cell ratios. However, IL-4 and IL-10 expression of PBMCs co-cultured with allogeneic MSCs significantly decreased while IL-17a and TGF-β expression increased significantly. In addition, our findings indicated that exosomes were capable of significant suppression at low PBMC ratios, regardless of autologous or allogeneic origin, but MSCs were more effective as the number of PBMCs increased. Conclusion: These preliminary findings from in-vitro experiments suggested that allogeneic MSC, or high-dose exosome administration may be a potential approach for treatment in CSU patients, most of whom are regarded as suffering from an autoimmune disease and resistant to current treatments. However, our findings need to be supported by clinical studies. Copyright © 2023 The Author(s).