Browsing by Author "Soykut G."

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    Apoptotic effects of Corchorus olitorius L. Leaf extracts in colon adenocarcinoma cell lines
    (Mattioli 1885, 2018) Soykut G.; Becer E.; Calis I.; Yucecan S.; Vatansever S.
    Corchorus olitorius L. is a highly consumed plant in Cyprus and other Eastern Mediterranean countries and known as a medicinal food in many cultures. The aim of the study is to determine in vitro anticancer and apoptotic induction effects of dichloromethane (DCM) and aqueous Corchorus olitorius L. leaf extracts in primary (Colo-320) and metastatic (Colo-741) colon adenocarcinoma cell lines.. Cell growth and cytotoxicity were measured with MTT assay with five different concentrations of extracts in Colo-320 and Colo-741 cell lines. Apoptotic activities of Corchorus olitorius L. were investigated by immunocytochemistry using antibodies directed against to caspase-3, cytochrome-c and FasLigand (FasL). TUNEL assay was used to detect DNA fragmentation in both cell lines. Both DCM and aqueous phase of extracts at 50 µg/ml concentration were more effective at inhibiting Colo-320 and Colo-741 cell growth when compared with other dilutions. The number of TUNEL positive cells was significantly higher in Colo-741 cells when compared with both control group and Colo-320 cell line. DCM phase extract significantly increased caspase-3 immunoreactivity while aqueous phase extract significantly increased cytochrome-c and FasLigand (FasL) immunoreactivities in Colo-320 cell lines. Both extracts were able to significantly increase caspase-3, cytochrome-c and FasL immunoreactivity in Colo-741 cells showing that both apoptotic pathways were triggered. Overall, Corchorus olitorius L. extracts induced apoptosis in both cancer cell lines while being more effective in metastatic colon adenocarcinoma cell lines suggesting that the extracts might have potential anticancer effects and possibility to be used as precursor to phytomedicinal colon cancer treatment as oppose to chemotherapy. © Mattioli 1885.
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    Obtaining stem cell spheroids from foreskin tissue and the effect of corchorus olitorius l. On spheroid proliferation; [Sünnet derisinden kök hücre sferoidlerinin elde edilmesi ve corchorus olitorius l.’nin sferoid proliferasyonuna etkisi]
    (Turkish Pharmacists Association, 2020) Becer E.; Soykut G.; Kabadayi H.; Mammadov E.; Çaliş İ.; Vatansever S.
    Objectives: Mesenchymal stem cells are self-renewing stem cells. The human foreskin has potential to be used as a source of stem cells. The aim of the study was to obtain spheroid formation of human foreskin cells (hnFSSCs) isolated from newborn human foreskin tissue. In addition, the apoptotic and proliferative effects of a traditional plant, Corchorus olitorius L. (C. olitorius), on hnFSSC spheroids were investigated. Materials and Methods: After a routine circumcision procedure the cells were isolated and cultured in suitable medium. The plant leaves was extracted with ethanol and their composition was analyzed by liquid chromatography coupled with mass spectrometry (LC-MS/MS). The foreskin stem cells were characterized immunocytochemically by CD45, CD34, and CD90 antibodies. hnFSSC spheroids were formed using the hanging drop technique. Immunofluorescence staining was used on the obtained spheroids to determine the distribution of caspase-3 and Ki-67 after being treated with C. olitorius extract for 48 h. Results: Immunostaining analysis showed that hnFSSCs were positive for CD45 and CD34 and negative for CD90. According to LC-MS/MS C. olitorius was rich in flavanols and hydrocinnamic acid derivatives. Although the spheroids obtained were loose and floating, the cells interacted with each other. Caspase-3 activity was higher in the control group than in the extract-treated group and Ki-67 was higher in the extract-treated group than in the control group, suggesting that the plant might have the capacity to increase stem cell proliferation due to its rich polyphenolic content. Conclusion: The results suggest that hnFSSCs and spheroids might be used in stem cell generation, tissue repair and renewal as human foreskin tissue has potential to be used as a stem cell source. C. olitorius also increased proliferation of hnFSSCs, showing that polyphenols might increase proliferation of stem cells. © Turk J Pharm Sci, Published by Galenos Publishing House.
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    Comparison of the Rate of Induced Intrinsic Pathway of Apoptosis on COLO-320 and COLO-741
    (Springer, 2021) Hincal E.; Soykut G.; Tijjani Saad F.; Vatansever S.; Abdullahi Baba I.; Çalış İ.; Kaymakamzade B.; Becer E.
    Mathematical modeling is a key tool in understanding complicated behavior of large signal transduction networks. In this paper a mathematical model is developed to understand the complex signaling behavior of induced apoptosis in Colo-320 and Colo-741 cell lines. The model studies the intrinsic pathway of apoptosis induced by Apaf-1, Cyt-c, and Caspase 3. Experimentally these tests were carried out five times for three groups; namely Control, DCM and Aqueous groups. The main aim is to compare the rate of the occurrence of apoptosis in the two cells Colo-320 and Colo-741 using experimental data. Numerical results reveal that apoptosis takes place at the same time in DCM for Colo-320 and Colo-741, but slightly faster in Aqueous group for Colo-320. It was observed that it took place between the 5–7th h in all the cases. © 2021, Springer Nature Switzerland AG.