Browsing by Author "Sunar M."

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    An immunohistochemical study of the effects of various antioxidants on rat lung during chemotherapy
    (Taylor and Francis Ltd, 2020) Yazici G.N.; Erdoğan D.; Gürgen S.G.; Sunar M.; Elmas Ç.; Umur N.; Ilgaz C.
    We investigated using immunohistochemistry the possible protective effects of ascorbic acid, α-tocopherol and selenium during chemotherapy treatment with cyclophosphamide. Thirty female Wistar rats were divided into five groups of six: group 1, untreated control; group 2, 75 µg/kg cyclophosphamide; group 3, 75 µg/kg cyclophosphamide + 150 µg/kg/day α-tocopherol; group 4, 75 µg/kg cyclophosphamide + 200 µg/kg/day ascorbic acid and group 5, 75 µg/kg cyclophosphamide + 40 ppm/kg/day selenium. Proliferating cell nuclear antigen (PCNA) staining was used to detect cell proliferation and AT1 was used to evaluate structural damage. Caspase-8, caspase-9 and caspase-3 signal molecules were used to investigate apoptosis. In group 2, epithelium, alveolar macrophages, infiltrated lymphocytes and connective tissue were immunostained moderately to strongly with PCNA. Bronchus, alveolar wall and infiltrated lymphocytes were immunostained moderately to strongly with AT1 and diffuse strong caspase immunoreactions were observed throughout the lung tissue. AT1 and caspase immunoreactions in groups 4 and 5 were similar to group 2. In group 3, PCNA immunoreactivity was strong in the bronchiolus epithelium, endothelial cell nuclei and in stacks of infiltrated lymphocyte cell nuclei. In group 3, AT1 and caspase immunoreactions were identical to group 1. It appears that α-tocopherol inhibits lung tissue damage in rats during chemotherapy. © 2020, © 2020 The Biological Stain Commission.
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    Effects of various antioxidants on rat lung tissue during chemotherapy: Electron microscopic study; [Kemoterapi Uygulamasinda Çeşitli Antioksidanlarin Siçan Akciǧer Dokusu Üzerindeki Etkileri: Elektron Mikroskobik Çalişma]
    (Gazi Universitesi, 2021) Yazici G.N.; Gürgen S.G.; Sunar M.; Elmas C.
    Objectives: This study uses transmission electron microscopy technique to investigate the efficacy of different antioxidants (such as ascorbic acid, alpha-tocopherol and selenium) in repairing or reversing lung damage caused by the possible adverse effects of the chemotherapy (cyclophosphamide) application on the lung tissue of the subjects. Materials and Methods: Thirty female Wistar rats were divided into five groups of six rats each: (I) control, (II) cyclophosphamide only (75 μg/kg), (III) cyclophosphamide (75 μg/kg) + ascorbic acid (200 μg/kg/day), (IV) cyclophosphamide (75 μg/kg) + α-tocopherol (150 μg/kg/day) and (V) cyclophosphamide (75 μg/kg) + selenium (40 ppm/kg/day). At the end of the experimental period the rats were sacrificed and the left lung of the subjects was removed and placed in a 2.5% glutaraldehyde solution in a 1/15 μ phosphate buffer (pH 7.4). The tissues were then stained with uranyl acetate and lead citrate to enhance the contrast, and examined and photographed with an electron microscope (Carl Zeiss 900 EM). Results: Alveolar type II cells were found to have degenerated in the cyclophosphamide-treated lung tissues. Vacuolization and crystolisis of mitochondria, disruption of the lamellar order and indications of apoptosis were observed. In the α-tocopherol group, mitochondria were normal and fibrosis was reduced. In this group, damage to the cell membrane and defects of lamellar bodies were present. Other groups produced similar results to the cyclophosphamide group. Conclusion: The results of our study showed that from all the antioxidants administered to rats during chemotherapy, only α-tocopherol was efficient in healing the tissue damage caused by cyclophosphamide. © Copyright 2021 by Gazi University Medical Faculty.